LOCUS       NZ_CP101929             1919 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Escherichia coli strain Seattle 1946 plasmid pEcFELIX377.4, complete
            sequence.
ACCESSION   NZ_CP101929
VERSION     NZ_CP101929.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29939549
            Assembly: GCF_024508155.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W. and
            Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W. and
            Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.92x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/17/2023 13:23:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 99 of 226
            Pseudo Genes (incomplete)         :: 137 of 226
            Pseudo Genes (internal stop)      :: 51 of 226
            Pseudo Genes (multiple problems)  :: 53 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101929.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Seattle 1946"
                     /culture_collection="ATCC:25922"
                     /culture_collection="DSM:1103"
                     /culture_collection="NCIB:12210"
                     /db_xref="taxon:562"
                     /plasmid="pEcFELIX377.4"
     gene            join(1774..1919,1..91)
                     /locus_tag="NP429_RS25220"
                     /old_locus_tag="NP429_25230"
     CDS             join(1774..1919,1..91)
                     /locus_tag="NP429_RS25220"
                     /old_locus_tag="NP429_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001676031.1"
                     /translation="MNRLTNLTPAEKKFLDDAIAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSEFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            317..499
                     /locus_tag="NP429_RS25225"
                     /old_locus_tag="NP429_25235"
     CDS             317..499
                     /locus_tag="NP429_RS25225"
                     /old_locus_tag="NP429_25235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          1437..1610
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(949..1470)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.308"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcgttacgcc gaccgccagc gtgcattacg tgaggatgag cggcagcagt ccgagtttgc
       61 ctggtcacgt ccgcgcgcgc cgcgccgctg attgcgcctt ttggcggttt agtggtgctg
      121 acactccgaa atcgtctgcc attcacccgc gtgcatagcc atgcaggtac gcagaaatca
      181 tcaggggcgt tttttctcga tatgggcaca gaaaggtgta tgtttcaggc cggaggcctc
      241 agaaacgcgc tgagggcgtt tgaggcggtg cggccaacgg atgttatgtt taatgttgat
      301 aacaggagta agagtgatgc ttacatttat tggtgtagtg gtagtcgccg ttattgcatt
      361 taaattattt atgctttttt ttagttcatc cgaatctgcc gtcgaacgtg ctacccgtaa
      421 atatatggag aaccctacga gtgctaacta taaaataatg gtggctacac gcatgagaaa
      481 tgagaagaaa agagggtgat atatgtttga gtttattggt tttttatttt tgcttttggt
      541 gtgttatgtc tttatacaaa tggttgctgt cagggtgttt cctgaatatg gagtacggaa
      601 agcggaggaa cgataccgtc gaaatccatc agcagaaaat gaggatctgt tatggagagc
      661 tagaagtcgt ataactaaac gcaagtaatg tttcctaagt gtgttgatag gcaaacatgg
      721 tgggggttac tactctcgga gttaatgatc ttcttgtgat cctgtcgttt cagtcgtaat
      781 ctcttgctct gaaaacgaaa aaaccacctg gggaggtggt ttgatcgaag gttaagtcag
      841 ttggggaact gcttaaccag gtaactgggt atacgagacc gcagataccg aaactgttct
      901 ttcagtgtag ccgcagtcag gcccatactt caagacctct gaatctctct ctcgtatatc
      961 ccagttacca gtggctgctg ccagtggcgc tttgtcgtgc tttctcgggt tggactcaag
     1021 atgatagtta ccgggtaagg cgcagcagtc ggactgaacg gggggtttgt gcatacagtc
     1081 cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga gataaacgcg
     1141 gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg
     1201 agccgccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat
     1261 ttgagcgtca ggtttcgtga tgcttgtcag gggggcggag cctatggaaa aacggcttcg
     1321 ccgcggccct atcgcttccc tgttaagtct cttcctggca tcctccagga aatctccgcc
     1381 ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
     1441 cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcgccggtg ccgccttttt
     1501 tctcctgcca catgaagcac ttcactgaca ccctcatccg tgccaacata gtaagccagt
     1561 atacactccg ctagcgctgc gtgactggtt cagggctgcg ccccgaaacc cgctaaaacc
     1621 actgccgcgc ctgcggcttg cccaacgtct cacgtcaccg gattaaaatc cgctcccgtt
     1681 caccgtttct ggctgtcttt cagcaccgcc agcgcttcat cggttctgct gtgtctggca
     1741 gactgtgcgg agttattctg acgggagaat gctatgaaca gactgacaaa cctgacacct
     1801 gcagagaaaa aatttcttga tgatgctatt gccgcagctg agagagcgtc aggcaaaaag
     1861 ctaaatcagc cgaaccggca tatcgtcctg aacagagcgc gtgcgcagat tgagtcgca
//
