LOCUS       NZ_CP101928             3173 bp    DNA     circular CON 18-AUG-2023
DEFINITION  Escherichia coli strain Seattle 1946 plasmid pEcFELIX377.3, complete
            sequence.
ACCESSION   NZ_CP101928
VERSION     NZ_CP101928.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29939549
            Assembly: GCF_024508155.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W. and
            Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W. and
            Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 213.92x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/17/2023 13:23:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,064
            CDSs (total)                      :: 4,948
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 99 of 226
            Pseudo Genes (incomplete)         :: 137 of 226
            Pseudo Genes (internal stop)      :: 51 of 226
            Pseudo Genes (multiple problems)  :: 53 of 226
            Pseudo Genes (short protein)      :: 4 of 226
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101928.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Seattle 1946"
                     /culture_collection="ATCC:25922"
                     /culture_collection="DSM:1103"
                     /culture_collection="NCIB:12210"
                     /db_xref="taxon:562"
                     /plasmid="pEcFELIX377.3"
     gene            complement(477..905)
                     /locus_tag="NP429_RS25195"
                     /old_locus_tag="NP429_25205"
     CDS             complement(477..905)
                     /locus_tag="NP429_RS25195"
                     /old_locus_tag="NP429_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(869..1039)
                     /locus_tag="NP429_RS25200"
                     /old_locus_tag="NP429_25210"
     CDS             complement(869..1039)
                     /locus_tag="NP429_RS25200"
                     /old_locus_tag="NP429_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            1197..1625
                     /locus_tag="NP429_RS25205"
                     /old_locus_tag="NP429_25215"
     CDS             1197..1625
                     /locus_tag="NP429_RS25205"
                     /old_locus_tag="NP429_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2014..2337)
                     /gene="mobA"
                     /locus_tag="NP429_RS25210"
                     /old_locus_tag="NP429_25220"
     CDS             complement(2014..2337)
                     /gene="mobA"
                     /locus_tag="NP429_RS25210"
                     /old_locus_tag="NP429_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            2621..2815
                     /locus_tag="NP429_RS25215"
                     /old_locus_tag="NP429_25225"
     CDS             2621..2815
                     /locus_tag="NP429_RS25215"
                     /old_locus_tag="NP429_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALNADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(2347..2583)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.49e-121"
     Typing          2..202
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.01"
                     /coverage="98.0"
                     /evalue="1.89e-97"
     Typing          3005..3173
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.647"
                     /coverage="91.0"
                     /evalue="2.08e-76"
     Typing          complement(2018..2337)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.23e-62"
ORIGIN
        1 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
       61 gcgctttctc atagctcacg ctgttggtat ctcggttcgg tgtaggtcgt tcgctccaag
      121 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      181 cgtcttgagt ccaacccggt aagacacgac ttaacgccac tggcaacggc cactggtaag
      241 caggatgtgc gagagatgta acgagagttc ttgaagtggc ggcctaactg aggctacact
      301 gaaaggacag tatttggtga catgtctcgc acaagactgt taccacggtt agagaatctg
      361 ccaggattaa ctaaccttcg aaaaaccacc tgccagggtg gttttttcgt ttttacaata
      421 gtgagattac gacgagactg aatgaatctc aagatttcat ttagcctgcc gctcgatcat
      481 tcaaagtttt gaggtagctt cattgggtca tggtatccaa gctctaaaag ctttatatgg
      541 ttcggtgtca gccgctggaa atgaataacc cagtcagaaa ccttgtattt cccatgaaac
      601 gtatctttaa aaaccggatc gcctaaatga gcatgccata atttgttagc tctagcatac
      661 ttctcaatag cttctttgtt ttcgtaatga tcaggcactt tccacgatgg cgatactttg
      721 ccagtccaag ctggatatcc ttttaaagga tcgccaaaga gattgtattt ttcaaaatgc
      781 tcaatgaaat catcgatttt atcgagtgtt tttgcagaat atctggatgt gtaataaagg
      841 ttgaaggtct cagagaatga gacaatattc aattgtggat tttgcgcgcc tgctccgctg
      901 cccatgctct tctctgctcc cgacttagac cagatggcat tgtaacagac ttctcattcg
      961 ctctggaaat caacagagac aaggatacgc caaaaacctc aatatcttta acaagatcat
     1021 gacgttgttc ggttttcata tcgctaactc cgtatctaat tcaggcagga aaaaatcttt
     1081 tcctcgtttt cagagtagtc ttttttggtt gcacaataat ggcgccagtg tcaccctatt
     1141 catatgaata taccatataa tatatacttt aaatattttg gggcttagga aaatcaatga
     1201 cttactccgt taagaataaa tggaaagctg gcggtttgtc tcttggctgg acagcagtgc
     1261 ctaccgtatt gttttttata caaaacgaga aaaagctaac ttcagtggcc tttaacacac
     1321 tacttaacct gatcgttcac tggtggagtt tgcaagagtg gccgcatccg tcaatggaaa
     1381 gtctagccat acgtatgggg gtttctgtac ggactgttca gagagctatc aatgatcttg
     1441 aaaaagctaa tctcttggac aagaaaccaa catcgaagag tgatcgccga tatggtggtc
     1501 gcaatattta tgatctaaca aagctagttg actacctaga tacaatgggt ccatcagtag
     1561 ctgaacaagt taagaaacca cgccataaaa aaccggtata taccgtaaga aaaacgacag
     1621 cttaaaaaag cacaatggaa ctgagcgcga gcgatgcacg atagcaaaac gatatattac
     1681 ttattgcatt ggtcttggta gccctgcttc tggctaaacc actgtcagga gtaattggct
     1741 ttgcctgtaa tgggttcatc gcttcacagt taaagttatc gcatagctca tatatttacc
     1801 ataatccctt aattgtacgc accactgaaa cgcgctgaac gcgattctga cgcagacacc
     1861 gcaaaaagag atgttttacc ccggaaaaac aggaaggatg cgtcaggatc gttttcagct
     1921 cgctgcatag ctatgcatga aagcgaatgg tgatcacttt gggagcttac ggtgttcata
     1981 ccgtcagttt tcgacagttt tctctccggg aagctaatct gccataagcc tggataacag
     2041 ggcacggtga tactccgtaa tagcgatcag cacctccgca tactccctgt ccccgacacg
     2101 cttgccgtcg ataaagagtt ttttctgtaa cgcccccagt ctcataactt ctttcagtac
     2161 ccgatcatca gtcagtgagt taactttctt accgagagct gccgcccgta aaaaaccgga
     2221 aacggtcttg ccgcattcag cagccctttt tctgatttcc tggtcttctt ctgccgtcag
     2281 acgaacaggg cgactgatag cccgcctgcg cgtattactt ccacttcgtt ttgtcataaa
     2341 acatggctcc gtatctgaca tgggtgtcgg ggcaaagccc tgaccagggc aattgtaata
     2401 gcgtgcatgt atgcgcggta taacaattgc acatcctgtc ctgtcagcaa gctcgaatta
     2461 ccgacaaagg cacggcagtt ttttggcagt gacagtgttg tctgacagaa atgcccgtca
     2521 aaaagccaag cggcagaata gcggcagcaa aacagtcgaa aagtggtacg cgatataatc
     2581 acacaacgca gatgacagac agaacggaga aaacgaaaag atgaataagc agcagcaagc
     2641 cgtactcaac atggcgggat tcataaaaag tcagagcctg acgctgctcg aaaaactgga
     2701 cgcactcaat gccgacgaac aggccgccat gtgtgagaaa ctgcacgaac tcgcagaaga
     2761 actccagaac agcatacaga cacgctttga agcggaaaat cttaccggga tataacgcat
     2821 ccggacagga tgcagaacgg catagaggag cgtaaggaga aaatgtcaca taaggcgctc
     2881 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtgtc
     2941 agctcactca aaggcggtaa tacggttatc cacagaatcg ggggataaaa ccggaaagaa
     3001 catgtgagca aaagacaaag acccagaaga agggcgcgcg ggaggcgttt ttccataggc
     3061 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3121 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tct
//
