LOCUS       NZ_CP101924             3327 bp    DNA     circular CON 07-SEP-2023
DEFINITION  Escherichia coli strain JCC-EE 7 plasmid pEcFELIX526.2, complete
            sequence.
ACCESSION   NZ_CP101924
VERSION     NZ_CP101924.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29939562
            Assembly: GCF_024507875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C. and Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C. and Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 214.09x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 04:52:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,677
            CDSs (total)                      :: 4,553
            Genes (coding)                    :: 4,310
            CDSs (with protein)               :: 4,310
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 65 of 243
            Pseudo Genes (incomplete)         :: 173 of 243
            Pseudo Genes (internal stop)      :: 49 of 243
            Pseudo Genes (multiple problems)  :: 43 of 243
            Pseudo Genes (short protein)      :: 3 of 243
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101924.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JCC-EE 7"
                     /db_xref="taxon:562"
                     /plasmid="pEcFELIX526.2"
     gene            complement(join(2664..3327,1..170))
                     /locus_tag="NP442_RS23275"
                     /old_locus_tag="NP442_23275"
     CDS             complement(join(2664..3327,1..170))
                     /locus_tag="NP442_RS23275"
                     /old_locus_tag="NP442_23275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072775497.1"
                     /translation="MILLAGKKMKTNYFTHRLFPSDGAVICEPLKTSFDNMIATNRNTA
                     TLANEYHVIQHVSGDVYTFIKTNSRDVFRKLDTSSNICVDLSNILNQHEKIAFASFFIL
                     KGDVIGFSNTLYSPRVGKLAEIYDAAMYSRNGNHNINFEPITNIVTEQDVMNYAHVGKI
                     TMKIEKSQGIIGGLGTLFSGNVKYDDVDSFEIKIIPKRAKDIKDTFAGLMQARPQEVSS
                     VAVSAKEHIGDVATDLNVIMSNTVYDFVNPNDTTTIEVQMEKNYNNNTTLRSLGY"
                     /name="hypothetical protein"
     gene            complement(1080..1229)
                     /locus_tag="NP442_RS23280"
                     /old_locus_tag="NP442_23280"
     CDS             complement(1080..1229)
                     /locus_tag="NP442_RS23280"
                     /old_locus_tag="NP442_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_163396971.1"
                     /translation="MPSEYDEQRDATDALLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(2216..2653)
                     /locus_tag="NP442_RS23285"
                     /old_locus_tag="NP442_23285"
     CDS             complement(2216..2653)
                     /locus_tag="NP442_RS23285"
                     /old_locus_tag="NP442_23285"
                     /inference="COORDINATES: protein motif:HMM:NF027298.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046597188.1"
                     /translation="MKLKGFWLSAIVIAACNGMGFLFLYLSKTYFFPMVTFKDNLGNLG
                     SSLVTYSTAMMALLIAMVVIIFGLDGPRLHNFKKSGYLHVTYFIYMITFFELGLTMALS
                     LLCLSNISSIKVASYSLTFAFITFLLIGCLGIQLLNIKKNL"
                     /name="hypothetical protein"
     Typing          complement(1223..1437)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.364"
                     /coverage="100.0"
                     /evalue="1.6000000000000006e-59"
     Typing          1387..1899
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.827"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 acatgctgaa ttacatgata ctcattggct agcgttgcag tgtttctgtt agtagcgatc
       61 atattatcaa aactagtttt taatggttca caaataacag cgccatctga aggaaacaat
      121 ctgtgtgtga agtaatttgt tttcattttt ttcccagcta gtaaaatcat agaaacgcat
      181 tatattttct tctttttata ttctatttct tatggaagaa caatgcttct ttaaaattcc
      241 aagttttgca tattaaatga tctgtcaccg aaggtcgtaa agctaggcca ccaactctat
      301 ttaccataac atccgttgcg tgcaccgccg cagaggccct cagcgcgttt ctgaggcctc
      361 cggcctgaaa cacacacctt tctgtgccca tatcgagaaa aaacgcccct gatgattctg
      421 gcgcgactgc atggctatgc acggacatac ctggccggga ttttagcggc taaaatccga
      481 tgaacgccct gaaagccacc acgaaccacc ccgataacgg tgaacggatg cgcaatttat
      541 tgcgtggccg tgaggcgttg ccggcacagc cggaaaaacc attttcagca cgtgcaggag
      601 gcccaggaag aggggcggat gtacgaatag ttcagaggtc agatctgacg taaaaatgat
      661 gtaaaagtga cgtaaaagtg atgtaatttt gatgtacttt tgatgtaatt ctgttgtaca
      721 cccgcgatct gacgtaaaag tgatgtaatt ttgatgtact tttgatgtaa ttctgtagtc
      781 aggaagaata acccgttcat gcaggcatga acgatacagt gaggaacgct ttttttgtca
      841 cgatcttccc ttaacctgcg taaatcaccc tctatggttg attatttttc aaccttaaaa
      901 tggtcataca ccatacgcaa ccgcgatttt catcgcaatt gcatgaatta aaaggtaaaa
      961 actgaagttc ccgtttattt atgtaataca caacgtatta caaccacctg taatcacatg
     1021 aaaataaaga aataagatgt aataccggcg tattacacgt acgcgggtaa gcgaaaaagc
     1081 tataacgctt cacggccgac aggtggccca aaatccaccg gggcgtgcag gttctcttca
     1141 gtaacctgcg ccattaaatc aaaaaggtca tattcgccgg gaagcagagc atcagtcgca
     1201 tcccgttgtt catcatactc agatggcatg ttgtgatatc cagtagctga ggggtgttag
     1261 cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt acagtggctg
     1321 aatatgttac ggcggagtaa cacctaatga aagtgcttca tgtgacaggg gaaaatgcag
     1381 ctacagggcg taagggagaa aatgggagtt acggtgatat gacgcctcct cgctcactcg
     1441 gtcgctacgc tcctgccgtg agactgcggc gggcgttacc tgctcacgaa tgacaagata
     1501 aaaagatggc actcaagtca ggaaaggccg gaaaaaggat acggcgctgc cgtttttcca
     1561 taggctccgc ccccctgaca agcatcacaa aatctgacgc tcaaatcagt ggcggcgaaa
     1621 cccgacagga ctataaagat cccaggcgtt tccccctggt agctccctcg tgcgctctcc
     1681 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc
     1741 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     1801 cccccgttca gtccgactac tgcgcctttc cggtaactgt cttcttgagt ccaacccgga
     1861 aagacacgac aacgcgccat tggcagcagc cattggtaag aggattcacg gagcagagta
     1921 gtagagtatt tctatggagt tcttgaagtg gtggcctgac tacggctaca ctaaaaggac
     1981 agttttggta tctgcgctcc gtgaaagcca gttaccttcg ggagaaagag ttggtagctc
     2041 tcacttccgg caaacaaacc accgttggta gcggtggttt ttttgtttac agagcaagag
     2101 actacgctgc actctcaaaa atctcaagac gatccttaca cattctgcat tttatcaact
     2161 caccaaaaat ggtcacaaac cgtttcacct gtgatgaaca tcacagtttg gttgattaaa
     2221 gattcttttt tatatttaag agttgaatac ctaaacatcc tatcaacaaa aaagtaataa
     2281 atgcaaaagt caatgaataa gaagccacct taatacttga gatattggac aaacaaagta
     2341 acgaaagagc catggttaag ccgagctcga aaaacgttat catataaata aaatatgtaa
     2401 catggagata gccgcttttt ttaaagttat gcaagcgagg tccatccaag ccaaagatta
     2461 taacaaccat agcaataagc aacgccatca tcgctgtcga ataagtaacc aaggaagaac
     2521 caaggttacc taaattatcc ttaaacgtta ccatcgggaa gaaataggtt tttgaaaggt
     2581 atagaaataa aaagcccatg ccattacatg cagctataac tatagcgctc aaccaaaaac
     2641 ccttaagctt cacaatgcaa tcctcagtaa cctaaacttc ttaatgttgt attgttatta
     2701 taattttttt ccatctgaac ctcgatagta gtagtgtcat ttggatttac aaaatcatat
     2761 acggtattag acattataac attcaaatct gttgcaacgt caccaatgtg ttctttagct
     2821 gacacagcga ccgagctaac ctcttgaggc ctagcctgca tcaaacctgc aaaggtatcc
     2881 tttatgtctt tagcccgttt agggattatc tttatctcaa acgaatccac atcgtcatac
     2941 tttacgttcc cagagaaaag cgtaccaagc ccaccaataa tgccttgaga cttctctatt
     3001 ttcatcgtta ttttaccaac atgggcataa ttcataacat cttgctcggt aactatgttt
     3061 gttatcggct caaaatttat gttgtggttc ccatttcggg aatacattgc agcatcatat
     3121 atttctgcca acttcccaac acgaggagag tacaacgtat tactaaaccc tataacatca
     3181 cccttcaata tgaaaaaaga tgcaaaggct attttttcat gttgatttaa gatgtttgag
     3241 agatctacac atatattact actcgtatct agttttctaa aaacgtctct gctatttgtt
     3301 tttataaacg tatatacatc gccagac
//
