LOCUS       NZ_CP101919             2095 bp    DNA     circular CON 07-SEP-2023
DEFINITION  Escherichia coli strain Evo1 plasmid pEcFELIX705.4, complete
            sequence.
ACCESSION   NZ_CP101919
VERSION     NZ_CP101919.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29939563
            Assembly: GCF_024508055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C. and Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C. and Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.39x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 04:56:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,718
            CDSs (total)                      :: 4,598
            Genes (coding)                    :: 4,363
            CDSs (with protein)               :: 4,363
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 72 of 235
            Pseudo Genes (incomplete)         :: 146 of 235
            Pseudo Genes (internal stop)      :: 55 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            Pseudo Genes (short protein)      :: 4 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101919.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Evo1"
                     /db_xref="taxon:562"
                     /plasmid="pEcFELIX705.4"
     gene            complement(501..692)
                     /locus_tag="NP443_RS23465"
                     /old_locus_tag="NP443_23435"
     CDS             complement(501..692)
                     /locus_tag="NP443_RS23465"
                     /old_locus_tag="NP443_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1724..1840
                     /locus_tag="NP443_RS23470"
                     /old_locus_tag="NP443_23440"
     CDS             1724..1840
                     /locus_tag="NP443_RS23470"
                     /old_locus_tag="NP443_23440"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_142293809.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKAISRVFRSKR"
                     /name="small membrane protein"
     Typing          568..1187
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.013"
                     /coverage="99.0"
                     /evalue="2.5900000000000003e-161"
ORIGIN
        1 ccgtgaggcg ttgccggcgc agccggaaaa accgttttca gcacgttcag gaaggggggg
       61 atgtacaaat ggcacagagg atggaagtga cgtatgagct gaaggaatga tgtaaaagtg
      121 atgcaaaagt gttgtaaaag tgatgtacag agcgaaggag tgttgtaaaa gtgatgtatg
      181 acctgaagga gcgatgtaat tctgtagtca ggaagaataa cccgttcatg caggcatgaa
      241 cgatacagtg aggaacgctt ttttttgtca cgatctcccc ttaacctgcg taaatcaccc
      301 gccatggttg attatttttc aacctcaaaa tggtcataca cctaacgcag ctgcgatttt
      361 catcgcaact tcatgaaaaa taaataaaaa agaaaaaata cagggattct gggtattacc
      421 ggggtaataa tgttaagcgc taacactatg aaaattaagg cgatgccggt attactccgg
      481 taatactcag aacggagaca ttacgcaccg tcctgctgct cattcaggcg cgctgacagg
      541 cttcggtaaa ggcgttccgc atcctcatgg agtttctcgc aaaaatcagc ctcatgatcc
      601 agatccagct cattaagtct gtccagaaga agcagggact gatcctggat gaatttagcc
      661 atgttaacgg cagtcttttg ttgtttgttc atgaaggccc cggcgcgaaa gtgcttactg
      721 tacgttctga tggttttccg cttcctcgct cattcgctca ctccgttcgg tcatgagtct
      781 gcggcgagcg gagggaggat aagcagataa cgtaacggta agcataacga aagctaagga
      841 caagggcgaa aaggccacgc cccgccgggg cgtttttcca taggctccgc ccccctgaca
      901 agcatcacaa aatctgacgc tcaaatcagt ggcggcgaaa cccgacagga ctataaagat
      961 cccaggcgtt tccccctggt agctccctcg tgcgctctcc tgttcctgcc tttcggttta
     1021 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     1081 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     1141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaatgcgcca
     1201 ctggcagcag ccactggtaa caggatgtgc gagagatatt gtgagagttc ttgaagtggt
     1261 gcccttacta cggctacact agaaggacag cttgggtgac acgtctcgca taagactgtt
     1321 accacggttc agaagttccc caacttactg aaccttcgaa aaaccgcctt gcagggcggt
     1381 tttttcgttt acagagaaag agattacgac gaggctgaat gaatctcaag atttcattta
     1441 gtctgccacc cgatcattca aagttttggg gtagttctga cctgcacccc gtgatttgtt
     1501 atatacccac cacgggggca ttagtttttt ttatttttat tttgatgata tgtacaaaat
     1561 cagtgattaa gaataatctt aacggcctta acaaacacat acaaaaacag tggctatgtt
     1621 tcatctcacc acaataagta acgacagtat catgataaaa aaaagatgat attatcttta
     1681 tgaaatcata acaggctgtt tttattaaat taatcaggca tttatggact ggatttcact
     1741 tgctatcgcc attgctctac ttgttgtagc tgtctactca ggaatctcat acttcagaga
     1801 aaaaaaagca atcagtcgtg tatttaggag taagcgatag cgccctattt accataatcc
     1861 cttcttacgc gcaccgcgaa aacggcctca gggcgtttct gacgggaaac cggtaaaaca
     1921 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     1981 gagtatgcgc gcaagtgcat agcgcagaat ttcacaccgt gcagccagtg gaagtcctgt
     2041 tttcgacgtt cttctcatcc gaaaacggtg aacggggacg caatttattg cgtgg
//
