LOCUS       NZ_CP101918             4404 bp    DNA     circular CON 07-SEP-2023
DEFINITION  Escherichia coli strain Evo1 plasmid pEcFELIX705.3, complete
            sequence.
ACCESSION   NZ_CP101918
VERSION     NZ_CP101918.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29939563
            Assembly: GCF_024508055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C. and Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C. and Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.39x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 04:56:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,718
            CDSs (total)                      :: 4,598
            Genes (coding)                    :: 4,363
            CDSs (with protein)               :: 4,363
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 72 of 235
            Pseudo Genes (incomplete)         :: 146 of 235
            Pseudo Genes (internal stop)      :: 55 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            Pseudo Genes (short protein)      :: 4 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101918.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Evo1"
                     /db_xref="taxon:562"
                     /plasmid="pEcFELIX705.3"
     gene            114..317
                     /locus_tag="NP443_RS23445"
                     /old_locus_tag="NP443_23415"
     CDS             114..317
                     /locus_tag="NP443_RS23445"
                     /old_locus_tag="NP443_23415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_079948274.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAIMCERLHELA
                     EELQNSIQTHFEAQYGIGVEQP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            868..981
                     /locus_tag="NP443_RS23625"
     CDS             868..981
                     /locus_tag="NP443_RS23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001712890.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(1145..1927)
                     /locus_tag="NP443_RS23450"
                     /old_locus_tag="NP443_23420"
     CDS             complement(1145..1927)
                     /locus_tag="NP443_RS23450"
                     /old_locus_tag="NP443_23420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079948273.1"
                     /translation="MTERILFVFEGEKTEKTITDSLLKKFIENDKRVVLSSFKTDIYSL
                     YKLMSEDEDLDVFAVLKEKNPDLVDFDRDSFSQLFLFFDYDGHAPAASDEKIDKLLSFF
                     NEETENGKLFISYPMVESIKCISHIDAIEDFCRHTVKICDCSKFKGYVAEYAHKSLIHF
                     NLYSDEIWNDVVRMHCVKSNFIMKGNMIFPSNYFSQKDIFGMQKSKYIDPNGSVSTLSS
                     FPMLLLDFFGHQRLFVLVSGEQIEDGDVLSSEEAQRTI"
                     /name="hypothetical protein"
     gene            complement(1920..2993)
                     /locus_tag="NP443_RS23455"
                     /old_locus_tag="NP443_23425"
     CDS             complement(1920..2993)
                     /locus_tag="NP443_RS23455"
                     /old_locus_tag="NP443_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418401.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_106121029.1"
                     /translation="MFDLTHRKNYEFNQDCILDGVVKKSLVYGKNGIGKSNLGLALFDI
                     VAHLTEFNVNDGMYNNYINAVNNSGIARFKYDFKFGDNIVTYLYGKRSVKELVFEELFI
                     NGRLVTKFNRVLDSNAYFSLSGTETLNTALAESKISIINYIKNNAILNDGIEKDLFNQF
                     IVFVEKMLYFRSLDSNNYLGLEMGRHLITPDIVERGNVDDFERFLNKAGVKCKLCVMKI
                     QSNNGVVEDIGFDFGSKQIPFYGVASTGTKSLALFYYWLQRFKDEKCVSFIFVDEFDAF
                     YHHSLSMLIVKEMKKAGAQVIITTHNTNVMTNELLRPDCYFIMDDKGITPLFEKTDKEL
                     REAHNIEKMYKAGTFND"
                     /name="ATP-binding protein"
     gene            3305..3547
                     /locus_tag="NP443_RS23460"
                     /old_locus_tag="NP443_23430"
     CDS             3305..3547
                     /locus_tag="NP443_RS23460"
                     /old_locus_tag="NP443_23430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157915800.1"
                     /translation="MQSLPLNASLNDLADREEPSMRKSFVTCRAIPIYHNVSLRAPPKR
                     AQRDSGAKTVKRGIFPLAKPEKCVRIVFSALHDYA"
                     /name="hypothetical protein"
     Typing          complement(4316..4396)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.683"
                     /coverage="89.0"
                     /evalue="5.6799999999999995e-25"
     Typing          450..937
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.684"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 atgaacggat gaacatcaaa caaccggaaa aacgctcact gtccaggcaa agaacggaac
       61 acggcataat cacatgatgc tgaatacaga gacagaacgg agaacgacag aaggtgaaca
      121 aacagcagca aaccgcactt aacatggcgc gattcatcaa aagccagagt ctgacactgc
      181 ttgaaaagct ggatgccctc gatgccgacg aacaggcaat catgtgtgaa cggctgcacg
      241 aactggcgga agaactgcag aacagcatac agacgcattt tgaagcgcag tacggcattg
      301 gagtggaaca accataaaga cgctggcgaa agcggtcgcg gcttgtccgt gagcgttttc
      361 gccagcagac aagcccgcag ggcgcgtcag gacagtgacc tgagaaagac actggcgcgt
      421 aagtgctcaa agcaaacggg cggagaaatt ccgcttcctc gcccactcgc tcactacgtt
      481 cggtcgctgg ggctgcggcg agcggtggcg gctctctaat aacggcaggc agtttatcca
      541 cagaatcggg ggataaccag gacagaacat gtgtgcaaaa gagcacgacc gtaaaaggct
      601 gcgacgatgc cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
      661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      721 agctccctcg tgcgctctcc tgttccgacc ctgccgttta ccggatacct gtccgccttt
      781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gttggtatct cagttcggtg
      841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      901 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacacgactt aacgccactg
      961 gcagcagcca ttggtaactg atttagagga gttagtcttg atgttatgcg ccggttaagg
     1021 ctaaactgaa agaacaagtt ttggtgactg agctcctcta ttctattagc cagttacctc
     1081 ggttcagaaa cttgccggtt actgaacctt cgaaaaaacc accttgccgg gtggtttttt
     1141 cttttcaaat agtacgctgg gcttcctccg aactcaatac gtctccatcc tcaatttgtt
     1201 caccgctgac cagcacaaac aatctttggt gcccaaaaaa gtctaaaagt aacatcggga
     1261 atgaacttaa tgtagataca gagccatttg gatctatata ttttgatttc tgcataccga
     1321 atatatcttt ctgtgaaaaa taattagaag gaaaaatcat attccccttc attatgaaat
     1381 tagatttcac acaatgcatc ctaaccacat cattccaaat ctcatcagaa tacaaattaa
     1441 agtgaatcaa tgacttatga gcatattctg caacataccc cttaaactta gaacaatcac
     1501 atatttttac tgtatgccta caaaaatcct caatcgcatc aatatgcgag atacatttta
     1561 tagactcaac cataggataa ctgataaata atttaccgtt ttcagtttcc tcattaaaaa
     1621 aacttagtag cttatctatc ttttcgtcag aagcagctgg agcatgccca tcataatcaa
     1681 agaacagaaa aagctgagag aaagaatctc tatcaaaatc tactaagtca ggattttttt
     1741 ccttcaacac ggcaaataca tcaagatctt catcctcact catcaactta taaagtgaat
     1801 atatatctgt cttaaatgaa gaaagaacaa ccctcttatc attctcaatg aattttttta
     1861 aaagactatc agtgatagtc ttctccgttt tctccccctc aaaaacaaat aaaatccgct
     1921 cagtcattaa atgtacccgc tttatacatt ttctctatat tatgagcttc tcgcaactct
     1981 ttgtctgttt tctcgaaaag tggagtaatc cctttatcat ccataatgaa gtaacaatca
     2041 ggcctaagca actcattcgt catgacattt gtattatgcg ttgtaattat cacctgggcg
     2101 ccggcttttt tcatctcctt aacaataagc atggaaagcg agtgatgata aaaagcatca
     2161 aactcatcta caaagataaa tgaaacacat ttttcatctt taaatcgttg caaccagtaa
     2221 taaaaaagag ctaatgattt tgtacctgtg gacgcaactc catagaatgg aatttgctta
     2281 ctcccaaagt caaagccaat atcttcaaca actccattat tactttgaat tttcattacg
     2341 caaagtttac atttaacccc tgctttattc aaaaaccttt caaaatcatc aacattacct
     2401 ctctccacga tatccggagt gattaggtgt cgccccatct ctagtccaag ataattattt
     2461 gaatcaagag atctgaagta caacattttc tcaacaaaaa caataaactg attaaataga
     2521 tctttttcga taccatcatt aaggatggcg ttatttttta tataattaat gatcgatatt
     2581 ttagactccg caagagcagt gtttaaagtt tctgttcctg ataatgagaa ataggcattt
     2641 gaatcaagca ctctattaaa tttagttaca agcctgccat tgataaacaa ctcttcaaaa
     2701 acaagttctt tcacactcct ttttccatat aaataagtta caatattatc accaaattta
     2761 aaatcatact taaatctcgc aataccagag ttgttaactg catttatata gttattatac
     2821 atgccatcat taacattaaa ctcagttaag tgagcgacaa tatcaaaaag agcaagccct
     2881 aagttgcttt tccctatacc atttttaccg tataccaatg attttttgac aacaccatca
     2941 agtatacaat cttgattaaa ttcataattc ttcctatgag taagatcaaa cactaatttg
     3001 tctgaaaacc ccttaaaccc ctcaacttca aatctgtata tcataaaagc ctcacatcaa
     3061 agatgccgta actatatcat ggcaccgtaa aaaaattaca ccaaatatag atattgattt
     3121 ttcttatcgc tgcaattaaa gttgcaagcg aaggctctcg cagtcctatg aaactcagca
     3181 tgcttgctgt tttttatagg tgtcagttcc tacaactgac gaagacagga gagtacaaaa
     3241 tgaaacgtaa aagcgtattc gttcgtgcct accgtcgctt tcgctacggc gagtgggaag
     3301 acgtatgcag tcattaccgc tcaatgcctc gctaaacgac ctggctgacc gcgaagagcc
     3361 ttcaatgcgt aaaagttttg taacttgccg tgccataccg atttaccata acgtctcatt
     3421 acgcgcaccg ccaaaacgcg ctcagcgcga ttctggcgca aaaaccgtaa aaagaggtat
     3481 tttccccctg gctaaaccgg aaaaatgcgt caggattgtt ttcagcgcgt tgcatgacta
     3541 tgcatgaaac tgaatggcaa tcggtctggg cgcgtccgct gtccctgagg cttatttttg
     3601 gcagttttct ttctgtctgc gtcacattcc atgccagaac acatccgtca gcaacgacat
     3661 tcaacagccc accattagcc cacacacaaa ataccgatgg gcacaccgat gtatcataaa
     3721 aatttatccg ttatatttca tacaaataag aggccaccat cagcccactc tattagccca
     3781 caaaacgccc acatttttgc tatcactatg attatcaata taaaacccac aaaagtgggc
     3841 tgatggtggg ctaactgtgg gcgtagaagc ccatcatcag cccaccacat ccgacaaaat
     3901 agaatttttt gccagataaa cgccactata accaactgaa tttaaagtaa aatcacgcaa
     3961 aacgcagttt tgcgacaggt gtggtaaatg caggtcagag gagaggggaa tttcaggcgc
     4021 ggagccggtc ctggttcggg ctacaggact ctcagaaaaa gcgaataaat ttgcgctggc
     4081 cgtcagagca ttacgccggg gtatcggggt gaaaccctga gcaggaggta attgtaatag
     4141 cgtcacgggt gacggtatta caattacaca tcctgtcctg tgtcaggcag ccggatacgg
     4201 tcgtcctggt ggcaacagtc cggcaggggg tacaactgta ccccgtcaca tccgcaaaag
     4261 atttacggca cgaaacagga gcgacgcaac aactgccggt aacacgaccc catgaacggg
     4321 acaggatgtg taatcgtata accgcgcacg cacgttcata cgattacaac ctggtcaggg
     4381 ctttaccccg acacccacga tgtg
//
