LOCUS       NZ_CP101917             6268 bp    DNA     circular CON 07-SEP-2023
DEFINITION  Escherichia coli strain Evo1 plasmid pEcFELIX705.2, complete
            sequence.
ACCESSION   NZ_CP101917
VERSION     NZ_CP101917.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29939563
            Assembly: GCF_024508055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C. and Celniker,S.E.
  TITLE     FELIX
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Wan,K.H., Park,S., Lawrence,Q., Eichenberger,J.P., Booth,B.W.,
            Piaggio,A.J., Chandler,J.C. and Celniker,S.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) BDGP, Lawrence Berkeley National Laboratory,
            1 Cyclotron Road, Berkeley, CA 94720, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 86.39x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/06/2023 04:56:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,718
            CDSs (total)                      :: 4,598
            Genes (coding)                    :: 4,363
            CDSs (with protein)               :: 4,363
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 72 of 235
            Pseudo Genes (incomplete)         :: 146 of 235
            Pseudo Genes (internal stop)      :: 55 of 235
            Pseudo Genes (multiple problems)  :: 38 of 235
            Pseudo Genes (short protein)      :: 4 of 235
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101917.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Evo1"
                     /db_xref="taxon:562"
                     /plasmid="pEcFELIX705.2"
     gene            complement(join(6208..6268,1..263))
                     /locus_tag="NP443_RS23405"
                     /old_locus_tag="NP443_23375"
     CDS             complement(join(6208..6268,1..263))
                     /locus_tag="NP443_RS23405"
                     /old_locus_tag="NP443_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001749507.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            327..518
                     /locus_tag="NP443_RS23410"
                     /old_locus_tag="NP443_23380"
     CDS             327..518
                     /locus_tag="NP443_RS23410"
                     /old_locus_tag="NP443_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1188..1349
                     /locus_tag="NP443_RS23620"
     CDS             1188..1349
                     /locus_tag="NP443_RS23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596883.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRARFI
                     QMLTEFLK"
                     /name="replication initiation protein"
     gene            complement(1541..2497)
                     /locus_tag="NP443_RS23415"
                     /old_locus_tag="NP443_23385"
     CDS             complement(1541..2497)
                     /locus_tag="NP443_RS23415"
                     /old_locus_tag="NP443_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640333.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="WP_224985182.1"
                     /translation="MKKLSSRYKRLFFMQRLSPGEFKTLISKERKSHFITPFALVYKTF
                     CDLGYDQKNSDYFLNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPY
                     DAIRDFTMEYPTHIYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFF
                     QKNQIGKLVDLVMPGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATL
                     DDSFSEETINILYEANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYT
                     DSEKEEIQQSILKQIEKYSDFPYVVNYYRNRLSALVD"
                     /name="type II restriction endonuclease"
     gene            2568..3707
                     /gene="dcm"
                     /locus_tag="NP443_RS23420"
                     /old_locus_tag="NP443_23390"
     CDS             2568..3707
                     /gene="dcm"
                     /locus_tag="NP443_RS23420"
                     /old_locus_tag="NP443_23390"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131511.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="WP_000131512.1"
                     /translation="MTDNIAATIKGKRERLHMTQKEFADALGLSKYGDRTIRRWERGET
                     KPTGAELKAVMDFPDTPPYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDK
                     FAQKTYHANYGDFPDGDITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFF
                     DIARIIKEKKPHAFLLENVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQ
                     NRERIYIVGFNKEKVRNHEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRK
                     LVNAAAGKGFGYGLFNENSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGF
                     PSDFIIPVSDTQAYKQFGNSVAVPVINAIAEKIISTLDS"
                     /name="dcm"
     gene            complement(3846..4289)
                     /locus_tag="NP443_RS23425"
                     /old_locus_tag="NP443_23395"
     CDS             complement(3846..4289)
                     /locus_tag="NP443_RS23425"
                     /old_locus_tag="NP443_23395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001605388.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            complement(4772..5005)
                     /locus_tag="NP443_RS23430"
                     /old_locus_tag="NP443_23400"
     CDS             complement(4772..5005)
                     /locus_tag="NP443_RS23430"
                     /old_locus_tag="NP443_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_004026237.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5012..5530)
                     /locus_tag="NP443_RS23435"
                     /old_locus_tag="NP443_23405"
     CDS             complement(5012..5530)
                     /locus_tag="NP443_RS23435"
                     /old_locus_tag="NP443_23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5568..6218)
                     /locus_tag="NP443_RS23440"
                     /old_locus_tag="NP443_23410"
                     /pseudo=""
     CDS             complement(<5568..6218)
                     /locus_tag="NP443_RS23440"
                     /old_locus_tag="NP443_23410"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          complement(604..689)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="3.99e-36"
     Typing          711..1260
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.756"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..293)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.938"
                     /coverage="82.0"
                     /evalue="3.54e-56"
     Typing          complement(4669..6218)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.802"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgatggcca tcagtgcggc caccacctga acccggtcac cggaagacca ctgcccgctg
       61 ttcaccttcc tcgccgtctg attcaggtta ttcccgatgg cagccagctg acgcagtaac
      121 ggcggtgcca gtgtcggcag cttcccggaa cgggcaaccg gctcacccag gcagacccgc
      181 cgcatccata ccgccagctg tttaccctca cagcgttcca gtaacctggc atgttcatca
      241 tcagtaaccc gtatcgtgag catcctctcg cgtttcatcg gtatcattac cccatgaaca
      301 gaaatccccc ttacacggag gcatcagtga ctaaacagga aaaaacagcc cttaacatgg
      361 cgcgctttat cagaagccag acattaacgc tgctggaaaa gcttaacgaa ctggacgcgg
      421 atgaacaggc cgatatttgt gaatcgcttc acgaccacgc cgatgagctt taccgcagct
      481 gcctcgcacg tttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      541 tcacagcttg tctgtgagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcag
      601 gttttagggg gtgtcggggc gcagccctga cccagtcacg tagcgatagc ggagtgtata
      661 ctggcttaac catgcggcat cagtgcagat tgtactgaga gtgcaccata tgcggtgtga
      721 aataccgcac agatgcgtaa ggagaaaatg cagcttccgg cgctcttccg cttcctcgct
      781 cactgactcg ctgcgctcgg tcgttcggct gcggcaagcg gtgccggctc actcaaaggc
      841 ggaaatacgg ttatccacag aatcagggga taaggccgga aagaacatgt gagtaaaaag
      901 gcaaagggca acaaaaggcc gcgccgctgg cgttttccca taggctccgc ccccctgacg
      961 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1021 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1081 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1141 gttggtatct cggcccggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1201 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1261 gacacgcctt aacgccactg gcagcagcca ctggtaaccg gattagcaga gcgagattta
     1321 tccagatgct tacagagttc ttgaagtgat ggcctaacta cggctacact ggaaggacag
     1381 tatttggtat ctgtgctctg ctgaagccag attacctcgg ttagataatc tgccaggatt
     1441 agctaacctt cgaaaaaacc acctgccagg gtggtttttt cgtttacagg tccaagatta
     1501 cgcgcagaaa aaaaggatct caagagatcc ttttagtttt ttagtcaaca agagcactaa
     1561 gcctatttct ataataatta acaacataag ggaaatcact atatttttct atttgtttta
     1621 atattgactg ttgaatttcc tctttttcag agtctgtgta acttacatta ttccactttc
     1681 ttgataattc cattgctgac tgtagcatat cttcaaaggt tagcacccta ttattatttt
     1741 tatatttgaa atttttattt tctatcgtcg ttacaacgac cacattagcc tcgtataata
     1801 tatttattgt ctcctcgcta aaagaatcat ctagtgtagc tagatacatt tctctaattc
     1861 ctgttctatt aacctcttca ggtacttctt gccatcgctc cctcagcgtt gttttagcgg
     1921 aaatcaacat tgtatttctt ttgttagatg tgtactggac tacacctggc ataacaagat
     1981 ctacaagttt acctatttgg tttttttgaa aaaatgactt cccaatagcc ccctgcacat
     2041 caacaggaat tcctgccccc atcattaata gctcaagaat agactcaaac tcttttcctg
     2101 ccctgctacg tctggattga gtgttagaca gtgccaaatc atatatgtgt gtagggtatt
     2161 ccatcgtaaa atcacgaatg gcatcataag gactcatatc ttttattctt tcttcatcaa
     2221 taagatatga taacattctt gttgtgaatt ctttttcaaa tggttcaaat tctttccaac
     2281 agttttttct cattgcaatt atatattcac ttggattatt caaaaaataa tcactatttt
     2341 tttgatcata ccctaaatca caaaatgttt tataaaccaa agcaaatggc gtaataaaat
     2401 gcgactttct ctctttagaa attagagttt taaattcacc aggactcaat ctttgcataa
     2461 aaaataacct tttatatcta cttgaaagtt ttttcatgca cgttcataat tggaatcaaa
     2521 acaggacaaa ttgtcctaaa accaacactt aattctggta gacatgcatg actgataata
     2581 tcgcagcaac aatcaaagga aaaagagaaa gacttcatat gactcaaaaa gagtttgcag
     2641 acgcccttgg tctttcaaaa tatggagaca gaaccataag aagatgggag cgcggggaaa
     2701 caaaacctac tggggcggaa ctaaaagcag ttatggactt tccagacaca ccaccttacc
     2761 caaacaacga gaatggcaga tatcgcatga tcgatttatt tgctggaatc ggtggaacca
     2821 gacttggatt tcatcaaact aacgctgtaa atgttgtttt ttccagcgaa tgggataagt
     2881 ttgctcaaaa gacatatcat gcaaattatg gtgatttccc tgatggggac ataacaaaaa
     2941 tcgatgaaaa agatattcct gatcatgaaa ttcttgtcgg tgggtttcct tgcgtagcat
     3001 tctctcaagc agggctcaaa aaaggattta atgatacaag aggaactttg ttttttgata
     3061 tagcaaggat aataaaggaa aaaaaacctc atgctttttt acttgaaaat gtaaaaaact
     3121 tgctaggaca tgataaaggt agaacttttt caataataaa aaacacactt gaagaattga
     3181 attacactgt gtattacaat atcttcgctg caaaagactt tggtgttcca caaaatagag
     3241 aacgcatata cattgtaggc tttaataaag aaaaagtaag aaatcatgaa cattttactt
     3301 ttccaacccc attaaaaaca aaaacaagag ttggagatat tcttgaaaag agcgtagaca
     3361 ataaatatac gctatcagat gcgctctgga atggacacca acgcaggaaa cttgtaaatg
     3421 cagcagccgg taaaggcttt gggtatggtt tatttaatga aaatagccca tatactaata
     3481 ccataagtgc gaggtactat aaagatggta gcgaaatatt aatagagcaa aaaggaagta
     3541 acccaagaaa aataactccg cgggaagcgt caagacttca agggtttcct agtgatttta
     3601 ttatccctgt atcggataca caggcttata agcaatttgg gaattcagtc gcagtccctg
     3661 ttatcaatgc aatagcagaa aaaatcatat cgaccttaga ttcataaact aagtcataag
     3721 gacacaatgc gacccagaat ccaattcccc ccccatatgg atttaccata acgtctcact
     3781 acacgaacca atgaaaaaag cccgcacatg gcgggctcac agggaaagta tcacagcgag
     3841 aacaatcaaa agttcatagc tttcttgcag ttgcgatagg ttgttggatg tagcgttcca
     3901 tagtaatcat cacggtcatc atcccaagcc cagctaatca aataagcctc agaatcatca
     3961 cttaccgtgc gagttctgcg tgtaagccct gaccagacac ctgaaagctc agagacatca
     4021 aaaccaagct cctttgcgag gctcttgcac cagaatcctc gctcaccatc ctcgctttca
     4081 agaatagctt tcagcactgt tcgcgactta tcacttaaac cacgaacaag cctttctgcc
     4141 tgaagcgagg aaagatcagt aggcccatct tccgactcct cataaacacc agaaaccgaa
     4201 tccccagtag ccaatgccag cacttcttct tttgcttggt ctgataacaa catcaaatct
     4261 tcacgggata agaaaatacc actagtcatg aaacactcca ttgtcttgtt aagataaaag
     4321 gagaataaac atagacaaga tagttgtcaa caagacaagt gacaagtcaa tacaactaac
     4381 atcaattaac agctagttgt gtttgattta ccgcattaac tgggttgcga tagtgagagg
     4441 tcaacactca acagcccgga tttaccataa cgtcgcatta cacgcaccgc taaaacgcgt
     4501 tcagcgcgat cacggcagca gacaggtaaa aatggcaaca aatcacccga aaaactgccg
     4561 cgatcgcgcc tgataaattt taaccgcatg aatacctatg caaccagatg gtacaggcca
     4621 cattaacccc tcttaatcca ctgaagctgc catttttcat gatttcacca tcccagcgaa
     4681 ggaccatcca gcgtgcgttc ctgtatttcc ggctgacgct cccgttccag ggataacgca
     4741 tgttcgcgct cctgtatcag ccgtgcctct cttatctcca gttctcgctg tataactggc
     4801 tcaagcgttc tgtctgctcg ctcaagtgct gcacctgctg actcaactgc atgacccgct
     4861 cgttcagcat cgcgttgtcc cgttgcgtaa gcgaaaacat cttctgcaat tccacgaagg
     4921 cgctctccca ttcgctcagc cgctgcatat agtcctgttg cagctgctct aaggcgttca
     4981 gcaaatgtat ttccagctct gtcactctgt gtcactcctt cagatgtacc cactctttcc
     5041 cctgaaaggg aatcacctcc gctgatttcc cgtacggaag gacaaggaat ttcctgttcc
     5101 cgtcctgcac aaactccacg ccccatgtct tcgcgttcag tttctgcaat gtctcttcct
     5161 gcttcctgat ttcttccagg ttcgtctgta tccgtccgcc aagataccag agcgtcccgc
     5221 cactcgcggt aaacaggaga aagactatcc ccagtaacat catgcccgta ttccctgcca
     5281 gctttaatac gtccttccgg tgctgcatca tcgcctcttt caccccttcc cggtgttttt
     5341 ccagcgattt ctctgtcgag gctgtgaaca gggctatagc gtctctgatt ttcgtctcgt
     5401 ttgaggtcac agcctcgctg acagactcgc cgagcttcct gaactcgtcg ttcagcattt
     5461 tctctgtaga ttcggctctc tctttcagct ttttctcgaa ctccgcgccc gtctgtaaaa
     5521 gattgctcat aaaacgctcc tttcagtctg atattcctcc cgccgttcgg gtctgcaatg
     5581 ctgatactgc ttcgcgtcac cctgaccact tcaagccccg cctctgtgag cgcctgaatc
     5641 acatcctgac ggccttttat ctctccggca tggtaaagtg cgtctatacc gcgtgtaacg
     5701 ctctcagcaa gtgcctgttt cgtttccggc aggttatcag gaagggtcag cgtccggcgg
     5761 ttctccgggg cgttggggtc atgcagtccg taatggtgat tcaccagcgt ctgccaggca
     5821 tcaattctcg gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc ggtctgtagc
     5881 tccatgttcg gaatgacaaa attcagctca agccgtccct tgtcctggtg ctccacccac
     5941 aggatgctgt actgattctt ttcgagaccg ggcatcagta cacgctcaaa actcgccatc
     6001 accttttcac gccctcccgg cggcagctcc ttctccgcga acgacagaac acccgacgtg
     6061 tatttcttcg caaatggcgt ggcatcgatg agttcccgga cttcttccgg attaccctga
     6121 agcaccgttg caccttcccg gtttcgctcc cttcccagca ggtaatcaac cggaccactg
     6181 ccaccgcctt ttcccctggc atgaaattta acaatcatcc cgcgctccct gttccctgac
     6241 gacctgccgt aagctgcgca actctctc
//
