LOCUS       NZ_CP101901             7832 bp    DNA     circular CON 10-OCT-2023
DEFINITION  Klebsiella pneumoniae strain 4011 plasmid pCol440I_4011, complete
            sequence.
ACCESSION   NZ_CP101901
VERSION     NZ_CP101901.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29397003
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Arcari,G., Carattoli,A., Bruno,F., Polani,R., Capitani,V.,
            Menichincheri,G., Sacco,F. and Raponi,G.
  TITLE     Ceftazidime-avibactam resistance in Klebsiella pneumoniae Sequence
            Type 37: a decade of persistence and concealed evolution
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7832)
  AUTHORS   Arcari,G., Carattoli,A., Bruno,F., Polani,R., Capitani,V.,
            Menichincheri,G., Sacco,F. and Raponi,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2022) Molecular medicine, Sapienza University of
            Rome, Viale di Porta Tiburtina, Rome 00182, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.8.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 31x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 10/10/2023 02:04:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 9
            CDSs (total)                 :: 9
            Genes (coding)               :: 9
            CDSs (with protein)          :: 9
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101901.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="4011"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pCol440I_4011"
                     /geo_loc_name="Italy: Rome"
                     /lat_lon="41.53 N 12.30 E"
                     /collection_date="2011-01-01"
                     /collected_by="Policlinico Umberto I Hospital"
     gene            join(6246..7832,1..1311)
                     /locus_tag="NKY01_RS00005"
     CDS             join(6246..7832,1..1311)
                     /locus_tag="NKY01_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(1661..2062)
                     /locus_tag="NKY01_RS00010"
     CDS             complement(1661..2062)
                     /locus_tag="NKY01_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_039817125.1"
                     /translation="MARVSISEASRLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKSEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="transcriptional regulator"
     gene            complement(2062..2253)
                     /locus_tag="NKY01_RS00015"
     CDS             complement(2062..2253)
                     /locus_tag="NKY01_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2662..2775
                     /locus_tag="NKY01_RS00045"
     CDS             2662..2775
                     /locus_tag="NKY01_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072016307.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPVRHAKAPLAAAIGN"
                     /name="replication initiation protein"
     gene            3124..3444
                     /locus_tag="NKY01_RS00020"
     CDS             3124..3444
                     /locus_tag="NKY01_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017134632.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_077255465.1"
                     /translation="MKIMSNQNIFEILTSDPVSLNMWNLKSNLILAIHKTVRDNGWNQT
                     EAAAHLGISQPRVSNMLKGKLDKFSTDSLLEIMFKLGYKLDMQVHPSSEVILEMGMKKA
                     ML"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(3464..3799)
                     /locus_tag="NKY01_RS00025"
     CDS             complement(3464..3799)
                     /locus_tag="NKY01_RS00025"
                     /inference="COORDINATES: protein motif:HMM:NF017757.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_077255464.1"
                     /translation="MSASEMKEICLDVTGVDKELKSLPIDKALAFLHNLKLVAAGIEPE
                     LKVEHLKVVAPGVIELKINGSPAYRCVYTIKSPGKVIVLRVFKKTTNGPARKDLEVVKQ
                     RLKNLKI"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4277..4537
                     /locus_tag="NKY01_RS00030"
     CDS             4277..4537
                     /locus_tag="NKY01_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein L"
                     /protein_id="WP_072016306.1"
                     /translation="MAAYTEKTEQFLQRVTHTQTWWTSTYDPSDTVPVSGIYRCTVCGK
                     EITSNKNDPFPPQNHHQHSQKQNVKWQLIVRTDTNGDKFGN"
                     /name="protein L"
     gene            4628..4927
                     /locus_tag="NKY01_RS00035"
     CDS             4628..4927
                     /locus_tag="NKY01_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_125564845.1"
                     /translation="MSNPPFEFVIRYHDLSPEQVRSFIDDAFHRVDLVLEKQLSDRPGF
                     FIPTCKSLTTNPSLSEIAIAVYNTYAVTEMIKDQLLRQSSNITLIKDIREIFVS"
                     /name="hypothetical protein"
     gene            complement(5546..6151)
                     /locus_tag="NKY01_RS00040"
     CDS             complement(5546..6151)
                     /locus_tag="NKY01_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509965.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGCAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     Typing          2302..2775
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.627"
                     /coverage="92.0"
                     /evalue="9.83e-170"
ORIGIN
        1 attccgcacg gtagcataga agatggaacg ttgcggatcg acaggttgac agcagacgtg
       61 ccggatggtg ccgaggcact catactggat ctgtatcgcc gaatgccgtc cgttcggatt
      121 accgacatgc tgcttgaagt tgatgcagcc cttggtttca cagatgcgtt tacccatctg
      181 agaaccgggg ctccatgtcg cgaccggatc ggtctgctca acgtcctgct cgctgaaggg
      241 ctcaatctgg gcctgcgtaa gatggcggaa gctacaaaca cgcatgatta ctggcagctc
      301 tcacgccttg cccgctggca tgttgaaagc gaagccatga accaggcatt ggcaattgtg
      361 gtggccgcgc agggtaaact gccgatgtca cgcgtctggg ggatgggcac gtcagcatcg
      421 agcgatggtc agtttttccc gacagcgcgg catggcgaag ccatgaacat ggtcaatgcc
      481 aaatatggtt ctgttcccgg cctcaaagcg tatactcacg taagcgacca gttcgcgcca
      541 ttcgcttgtc agtcgatccc ggcgaccgtg agcgaggcac cgtatattct cgatggacta
      601 ctgatgaacg aggtcggtcg ccatgttcgc gaacagtatg ccgatacagc aggattcacc
      661 gaccatttgt tcggagccag tagcctgctc ggctacaatc tcgttctgcg aatcagggat
      721 ctgccatcga agcggttgta cgtatttaat cccgatacga cccccaggga gttacgcaag
      781 ttggtaggtg gaaaagcccg ggaggatctt atcgttgcga actggcctga tattttccgt
      841 tgtgccgcga cgatgaccgc tggcaaaatc aggcccagcc aactcctgcg caagctcgct
      901 tcttacccac gacaaaacaa ccttgcagtt gcgcttcgtg aagttggtcg tattgaacgg
      961 acccttttca ttattgagtg gatcctggat acggacatgc agcggcgtgc tcagatcggt
     1021 cttaacaagg gagaggccca ccatgcgctc aaaaatgcgc tccgtatcgg gaggcagggg
     1081 gaaattcgcg atcgcacgac agaggggcag cactaccgaa tcgctgggct caatttattg
     1141 actgcggtga tcatttactg gaataccgtc catcttggtc atgccgtcac ggagcggcgg
     1201 aacgaagggt tggatgttcc ccctgaattt cttccccaca tatccccatt gggctgggcg
     1261 cacattctac tgactggcga atatctttgg cccaaggaac cgaaagctta gggtgtcatt
     1321 tcgccctcag ccggaaccga ccccaaacgg ggagtcgggt ctagagtcgg gtcgttgtta
     1381 acttattgtt tttatagtcc tgagtcgtta ccgactctat gacgactcgc cggggactct
     1441 atgacgactc gtcatagagt cggatctgta taaataaaat taaaaaaacg aaatttatag
     1501 aacgtaagct gctgatataa aatacaaatc tgcaaaattt taattttgcg tggggtgtgg
     1561 atatttttag tggtgaggag cgttcagaga tctggcgatt tttgggaagg ttcaggaact
     1621 ccccgggatg gccggagagg aaaactgaag catgacgggg tcaggatttt ttccagaatt
     1681 tccaccatgg cgccggggat tcagttaatt ttgcctggtg attttcaaga aggagcatgg
     1741 cctgtttcag gctatcaatg tgcgaatcct tggacaaaag taaggtgttc aggtgttcaa
     1801 tctcctgttt cagatgacga atcacctgtt cactctggtc agcagaggct gtatcacgct
     1861 gttcagagac ctgtccagaa tggtgttcac tcttcggttg ctcaaaggga ccaaagacac
     1921 gtaaaagctc tgaagtatct accatttttg cattacgttc accgctacag gtggacaggt
     1981 cacctgcttt aattaacctg tgcagtgttg ttctactctt acccgtcagt cgggaagcct
     2041 cactaataga cactcgggcc atcagttacc atcctgtaat gcatccagtc tcgaggacag
     2101 tgtacggaaa agatgctccg catcatcgtg gagtttctca caaaggtcag cctcagcatc
     2161 gagatcaagc tcgttaagct tctccagcag gagcagggac tgattctgga tgaacttagc
     2221 catgttcagc gccgttttct gttgtttcgt cataaagggc ctcagagcgt cagtgctcaa
     2281 tgtaaaaccg gtctcttttc cgcttcctcg ctcactcagt cgctacgctc ctaccgttcg
     2341 gctgcggcga gcggtcgcaa cgcacacata agggcatagg acagacagct cggaaaggac
     2401 aacggaaggc cgcggcgttg ccgtttttcc ataggctccg cccccctgat aagcatcaca
     2461 aaaacagacg ctcaaatcag tggtggcgaa acccgacagg actctaaaga taccaggcgt
     2521 ttccccctga tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     2581 ttccgcagtt atggccgcgt ttatctcatt ccacgcctga cactcagttc cgggtaggca
     2641 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctta
     2701 tccggtaact atcatcttga gtccaacccg gtaagacatg caaaagcgcc actggcagca
     2761 gccattggta actgattaga ggagtgaagt cttgaaaaga cacgtactat acggctacac
     2821 tgataaaaca gatttggcga ctgtgctcct ctaagccagt tacctcggtt cataaacttg
     2881 ccggtttgat gaaccttgaa aaaccgcctc cccaggcggt ttttcttttt aaaacaaatg
     2941 aagactacgc tgacgaccaa gatccagtca acaagtgttg ttagaatgct ttaaatttac
     3001 aaataaaacc ccatctacat cttgtcatgc atactgtaac ttgtataatc cgcaaacttt
     3061 agtgtaagct aataatcgtt cctattgtct tgtgacaact aaatgctcgc attcagatag
     3121 gagatgaaaa ttatgagcaa tcaaaacata ttcgaaatac taacgagtga tccggtatcc
     3181 ctaaacatgt ggaacctgaa atcaaacctg atcctcgcca tacataagac ggtaagagat
     3241 aacggctgga accaaacaga ggcggccgca catctaggca taagccaacc tagggttagc
     3301 aacatgctga aagggaagct ggacaagttt tctacagatt cactactgga aatcatgttc
     3361 aagctgggtt acaaattaga catgcaagta cacccatcct ctgaagtaat tttagagatg
     3421 ggcatgaaaa aggcgatgct gtgaacatcg ccttgagtgg atactatatt ttgagatttt
     3481 tcagccgttg cttcacgact tctaagtctt tgcgtgcagg tccgttagtc gttttcttga
     3541 agacacgtaa cacgatcacc ttgccgggtg attttatcgt gtacacacaa cgataagcag
     3601 ggctaccatt tatcttcaac tcaatcacgc ctggagcaac tactttcagg tgttcaactt
     3661 tcagttctgg ttcaattcca gcggcaacca acttcaagtt gtgcaaaaaa gctaaagctt
     3721 tatcaatagg taaagacttt agctctttat caacaccagt tacgtctaga caaatctctt
     3781 tcatttcgct tgcggacata gtgtcctcct ttgttggggt ttagtggtcg aacaattaaa
     3841 tttctcattt gatactccga ttaaaaaaac ctcactaaaa aaccaaacct ctacgcctga
     3901 tgagattcac caggtagagg catttaattt cagtggttca ggacactgct ttaggcaata
     3961 tccattgcat caatatactc taagttgtat aaactaacaa tatgccgctt cgcttgcgca
     4021 tgccgttagc tcaataaata agctatcagc tgtttacgaa agtaatcctc catcgaagct
     4081 aaaagtctcc gtaaagcatc acaactgaaa gaaggagtta agagatggca gatagaagac
     4141 cgctaaacaa agatgtggta gtgccaaagc ctggtggcgg aacccagccc agagacagaa
     4201 acaatgacgg aacggtgcgc aaaaaacgct cagacgcagg aaaaccaaga aaataaacga
     4261 taagaaaggt aaaagaatgg ctgcatatac ggaaaaaaca gagcagttct tacagcgagt
     4321 tacacacaca cagacttggt ggacatcaac atacgatcca agcgatacag ttccagtatc
     4381 aggcatttat cgctgtaccg tatgcggtaa agagataact agtaataaaa acgatccttt
     4441 ccccccacaa aaccatcatc aacatagtca aaaacaaaat gttaaatggc agctaattgt
     4501 gaggacagac acaaacggtg ataaatttgg taactgagac acggaagccc ttcggggctt
     4561 tccctcagtg atttaccaga aagtctcatt actcactcgt accaaacaat ccacgatctg
     4621 aggtcttatg tctaatccac cttttgaatt tgtcattcgt tatcatgacc tgtctccaga
     4681 gcaagtgcgg agctttattg acgatgcttt tcatcgtgtt gatctcgttc tagaaaaaca
     4741 gttatccgat cgtccaggct tttttattcc tacatgtaag tcactaacta ctaatccgtc
     4801 tttatctgaa atagctatcg ctgtttacaa cacatatgcc gttactgaaa tgataaagga
     4861 ccaattgctt cgacaatctt ccaatatcac tctcattaaa gacataagag agatctttgt
     4921 cagctaggat cttctctacc acaccacaag tgcttatctc ataacagctt atttggccaa
     4981 acaagactgg cgcagaggga gtttccgaac aggtcgccgg gaggtggacc gcttcgcacc
     5041 tcagcaggaa atccatcaga ccaatcgggg ctttttacat ctatcattta acataaaggt
     5101 atgattgtac gcaccgctaa aacggcctca gggcgtttct gacgggaaac cggtaaaaca
     5161 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5221 gagtatgcac gcaagtgcat agcgcgaaaa ttcacgccat tcagccaggg taagtcctgt
     5281 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5341 aagcgttgcc ggcgcagccg gaaaaccggt tttcagcacg ttcaggaatc gggttcgtcg
     5401 acggccggca atcacgttat ggcaagtcgc cggacgactc accacacgat tcaccggcga
     5461 ctcttcacta gagtcgtacc cagagtcgtc acgtctaaaa aacaaccaaa cgggggtcgg
     5521 ttccggctga gggcgaaatg acaccctaag cgttagctct gtgtcgttgc acgatgtcag
     5581 cgacggtatt cttgctgata ccgagctcgc gtgcgatcca gcgatagctg cgtccctcgg
     5641 ccctcatcgc aaccacctta ggcaaaagtc ggtctgattt tggtcgcact ccggcctgac
     5701 gaccaagcct cttaccacgt gccttcgcaa cagcaaggcc tgacttgacc cgctcgctga
     5761 tgagatcccg ctcaaactcc gcaatgccgg aaagaaacgt cgccagcatt cgtccatacg
     5821 gcgacgaaag atcgaacgcc attccattca tggctatcac ggaaaccttc cagttctcca
     5881 gttcacgtag cgtattgagc agatcgagcg tcgagcgccc ccaccgggaa agctcagtga
     5941 ccaggattgc atcaatttgt ctggactggg caagcgccag gactttcttt cgctcggccc
     6001 ggtcgagttt agttcctgaa cctgtttcct taaatattcc caccacgtcg tagccggcac
     6061 agccggcgaa ggctcgcaga tcaaattcct ggcgttcaca agactgatcc gctgttgaaa
     6121 cccggcagta aatggcggca cgatgtccca attgaaccct cctggatttt tgtatcggaa
     6181 cgccctgatt tatatgggct ggctgttgtc caaaacagac tatacttcaa aagggacgaa
     6241 tttgtatgtc acgacgccat attttcaccg aacggcagcg agcagcgctg ttcgatctgc
     6301 ccacggacga actgtcgcta ctgaagttct acacgctggg cgatgatgac ctggaaaaca
     6361 ttaggcagcg ccgcagaccg gaaaacagga ttggctttgc cctgcaactt tgtgccttac
     6421 gatatccggg ccgtgcactg gctcctggtg agatgatccc gcgtgaagtc ctttccttcg
     6481 tcggtgctca gcttggagtt ccggctgatg cgcttctcac ttatgccaca cggcgccaaa
     6541 cccgtcagca gcacatggac acgctgcgcg aaatttacgg ctacaagacc ttcacgggcc
     6601 gtggtgcccg tgatctgcgg gagtggactt tcggccaggc cgaagatgcc agatcaaacg
     6661 aggatcttgc tcatcgtttt attgtgcggt gtcgggaaac ttccaccatt ctgcccgcag
     6721 tatcgacaat cgagcgcttg tgcgcggatg ctctggtcgc cgctgagcgg cggattgaaa
     6781 cgcggattgc ggaaaattta acagcggatg ttcgcgatca cctggacaaa cttctgagtg
     6841 aaatgctcgc cggcaatatc agtcgtttca tctggcttcg caacttcgag gttggtaaca
     6901 actcggctgc tgctaaccgt ttgctcgaca ggctcgaatt tctgcgtacc ctgaatatca
     6961 atcatagtgc tttggccagc atacctgccc atcgcattgc ccggctgcgt cggcagggtg
     7021 aacgctactt caccgacggt ttgcgtgaca tcacttcgga ccgccgctgg gcgatccttg
     7081 ccgtctgtgt tgtggagtgg gaagcggcga ttgctgatgc catagtcgaa acccatgaca
     7141 ggatcgtagg aaaaacctgg cgggaagcga agcgccagca tgacgaaaca atttccggct
     7201 ctaaagccac actcacggat acgatccgta ccttcaccgc gctgggagct tcgttgcttg
     7261 aggcccgcag tgacggaacc ccgctggaga tggctgtcgc cagttcggtt gcatgggacc
     7321 ggctcgctca actggtagcg acagggactc aactcagcaa cacgctagcc gatgagcctc
     7381 ttgcatatgt cgggcaggga taccatcgct ttcgtcgtta tgcgccccgc atgttgcgct
     7441 gtctgaagct cgaagccgcg ccggtcgccg gaccattggt agcagcagct ttgtcgatcg
     7501 gagagatgaa aggtgttgca tcgccagaaa ggcgtttcct gcggcccagc tccaaatgga
     7561 accgtcattt acgagctcag gaaaaaggag atacccgtct ttgggaagtg gcggtactct
     7621 ttcacctccg ggatgctttt cgttccggag atgtctggct cgctcattcg cgccgctatg
     7681 gtgacctcaa gcaggtactg gtgccgatga tcgcggcgca ggaaaatgca aaactggccg
     7741 tgccttccaa cccacaggat tggctggcag acagaaaggc gcgactcacg atcgctctta
     7801 agcggctggc ccgggctgcc cgtaacggca ct
//
