LOCUS       NZ_CP101900             9294 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Klebsiella pneumoniae strain 4011 plasmid pColRNAI, complete
            sequence.
ACCESSION   NZ_CP101900
VERSION     NZ_CP101900.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29397003
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Arcari,G., Carattoli,A., Bruno,F., Polani,R., Capitani,V.,
            Menichincheri,G., Sacco,F. and Raponi,G.
  TITLE     Ceftazidime-avibactam resistance in Klebsiella pneumoniae Sequence
            Type 37: a decade of persistence and concealed evolution
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Arcari,G., Carattoli,A., Bruno,F., Polani,R., Capitani,V.,
            Menichincheri,G., Sacco,F. and Raponi,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2022) Molecular medicine, Sapienza University of
            Rome, Viale di Porta Tiburtina, Rome 00182, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.8.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 31x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 10/09/2023 02:58:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101900.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="4011"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColRNAI"
                     /geo_loc_name="Italy: Rome"
                     /lat_lon="41.53 N 12.30 E"
                     /collection_date="2011-01-01"
                     /collected_by="Policlinico Umberto I Hospital"
     gene            complement(join(9216..9294,1..185))
                     /locus_tag="NKY01_RS00005"
     CDS             complement(join(9216..9294,1..185))
                     /locus_tag="NKY01_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(491..910)
                     /locus_tag="NKY01_RS00010"
     CDS             complement(491..910)
                     /locus_tag="NKY01_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1313..2998
                     /locus_tag="NKY01_RS00015"
     CDS             1313..2998
                     /locus_tag="NKY01_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3008..3265
                     /locus_tag="NKY01_RS00020"
     CDS             3008..3265
                     /locus_tag="NKY01_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            3350..3499
                     /locus_tag="NKY01_RS00025"
     CDS             3350..3499
                     /locus_tag="NKY01_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4980..6944
                     /locus_tag="NKY01_RS00030"
     CDS             4980..6944
                     /locus_tag="NKY01_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            6944..7675
                     /locus_tag="NKY01_RS00035"
     CDS             6944..7675
                     /locus_tag="NKY01_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            7682..8212
                     /locus_tag="NKY01_RS00040"
     CDS             7682..8212
                     /locus_tag="NKY01_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8240..8419)
                     /locus_tag="NKY01_RS00045"
     CDS             complement(8240..8419)
                     /locus_tag="NKY01_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8488..8865
                     /locus_tag="NKY01_RS00050"
     CDS             8488..8865
                     /locus_tag="NKY01_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(8927..9226)
                     /locus_tag="NKY01_RS00055"
     CDS             complement(8927..9226)
                     /locus_tag="NKY01_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     BGC             1..7998
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3603..4181)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6945..7672
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tcagcacggg cggcggtcac tttggcgcga acctgatctt catactctgc ctgggccagt
       61 tcttcgttag ccagtcgctg caacagcgca gctttgtccg ggccaccttc cggcagtatg
      121 cggggcaggc tttcagccag ttcccgaaga aagttggcgt tttcgtagtg tgtagtgttg
      181 ctcattgcgg agcctcccgg gttaatgcgg caatatgaag gaagtttagc agggggcggt
      241 ctgaaagaat atggtcgtgt cagtcaggct gatgaagcag aaagtcggta aaaaagggct
      301 tacggcatcc attttacgtc aaaacatatg ccatgcagtt gcgtgcatag gcatgcatta
      361 ggataaaatt tatcgggcgc gtttccggca gttttccggg ggggttgttg cctgttttat
      421 cccgtagccg ccggaaacgc cctgagcccg tctgagcggt gcgcgtaatg acgcgttatg
      481 gtaaatccac ttactgtctg ccttcatagc catcgagata aacagctgaa atggtgtcag
      541 ccagtagcct tgattttttc ggtgtgaggt ctttgctgcg gtaatcggta cccgcaaatt
      601 gtgttgtgcc ttggagtgag tttaaaaatt cctgactgtg aaactgctga atgcgttgct
      661 gcacatcact ccgtgatttt ccggtcgctt tatcctttgc gcccatatcc cagaactgcc
      721 ggaaaaaagg caccatcatt cgtgttgtgt cagctttctg ggtggaaaaa ttgggatcaa
      781 aaccgtcatc tgaagcatat acgtaatgcc gggtatgctg ttccacgctg ccctttggag
      841 cacaggcagt gagagttgct gtcgctgtta tcatggcgat aagacacagt ttgctgccgg
      901 aacgtctcat gagcgtctcc ttacctttaa ttcttttttc tcattgagtt atatatccgc
      961 aggctatctt atcatcagtt tctgaccgtg ttcagtgacc gaacaacccg aaccagaccc
     1021 cccgtttctt ttcctgtccc ggagggggct ttctggtatc tcatataatg atttcagtaa
     1081 aacatactga ggccggagaa cggcgccgtt tgtgctggtt tttgttgcat ctggagggga
     1141 ttgcgcatgg tgtgagtggc tgtttttttg atcattttca caaaaacgca aatttacaga
     1201 cttgacatga aaaacaaggc agcgtaattt ttgtactgta tataaaacca gtggttatat
     1261 gtacagtatt ttattttagt ttattgtttt aaaagtcaaa gaggaaaagt atatgagtgg
     1321 cggagacgga cgaggtccgg gtaattcagg tctgggacac aatggtggtc aggccagtgg
     1381 gaatgtgaac ggtacatcag gtaaaggtgg cccttcatca ggtggtggta cggatccaaa
     1441 cagcgggccg ggctggggta cgacgcatac acctaacggg gatattcata actataaccc
     1501 gggggagttt ggtaacggcg ggagtaaacc cggtggtaat ggcggtaaca gcggcaatca
     1561 tagcgggagc tctggtggcg gacagtcttc ggccaccgcg atggccttcg gtctgcctgc
     1621 cctggccact ccgggggctg aaggactggc tttatccgtt tccggcgatg cgttgtcggc
     1681 cgctgttgct gatgtgctgg ctgccctgaa aggtccgttt aagtttggtc tgtgggggat
     1741 tgcgatttac ggagtgctgc cttctgagat agcaaaagat gatccgaaaa tgatgtcaaa
     1801 aatcgtgacg tcattgccgg cagacacggt aacggagacg ccggtcagct ccctgccgct
     1861 ggaccaggcg acggtcagcg tcactaaacg tgtggcggat attgtgaagg acgagcggca
     1921 gcatattgcg gttgtcaccg gccggccaat gagtgttcct gtggtggatg cgaaaccgac
     1981 aaaacgtccg ggggtattca gtgtgtcgat tccgggtctc ccgtctctgc aggtgagcgt
     2041 acctaaaggt gttccggccg cgaaagcccc gccaaaaggc attattgctg aaaaaggtga
     2101 ttcacgtccg gctggtttta cggccggtgg taactcccgt gaggccgtta ttcgtttccc
     2161 gaaagagagc ggacagaaac cggtctatgt gtcggtgacg gatgttctta ctccggcaca
     2221 ggtaaaacag cgtctggagg aagaaaagcg tcgccagcag gcatgggacg ctgctcaccc
     2281 ggaagagggg ctgaaaagag agtatgataa agcgaaagct gagctggatg ctgaagataa
     2341 aaatattgcg accttaaaca gtcgcattgc atcgacagag aaggcgatcc ccggtgcaag
     2401 ggctgctgtt caggaagccg ataaaaaggt aaaagaggca gaggcaaata aagatgattt
     2461 tgtgacttat aacccgcctc atgaatatgg ctccgggtgg caggatcagg ttcgctatct
     2521 tgataaggat attcagaatc agaatgagaa attaaaagct gcccagacat ctttaaacga
     2581 aatgaatgaa tccttatcca gggataaggc tgcgctttcc ggggcgatgg agagccggaa
     2641 acaaaaggag aaaaaagcga aggatgcaga aaataaatta aatgaggaaa agaaaaaacc
     2701 tcgtaaggga actaaagatt acggccatga ttattttcct gatcccaaga ctgaagatat
     2761 taaggggttg ggagagttga aagagggtaa acctaaaacc cctaaacaag gtggtggtgg
     2821 taagcgggcc cgatggtatg gtgataaaaa gcgtaaaatt tatgaatggg attcccagca
     2881 cggtgagctt gaagggtacc gcgccagtga tggcgaacac ctcggggcat tcgatccaaa
     2941 aacgggtaag caggttaaag ggccggatcc aaaacgaaac attaaaaaat atctttaaga
     3001 ggtaaatatg gggcttaaat tacatattca ttggtttgat aagaaaaccg aagagtttaa
     3061 aggtggtgaa tactcaaaag acttcggtga tgatggttct gtcattgaaa gtctggggat
     3121 gcctttaaag gataatatta ataatggttg gtttgatgtt gaaaaatcat gggtttcgat
     3181 attacagcca cactttaaaa atgtaatcga tattagtaaa tttgattact ttgtatcctt
     3241 tgtttaccgg gatggtaact ggtaagcaaa agttatcaag gatgatttga aatacagact
     3301 tccggtgttc agggatgaat accggggttt ttaaatacag ggaaggtata tgaaaaaggt
     3361 aaaggttgtt tttcttttta tattgatttt ttcaggcttt cttctggtcg catgtcaggc
     3421 aaactatatc cgggatgttc agggtggaac ggtggcacca tcgtcctcct ctgaactgac
     3481 ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3541 gtaatctttt gccctgtaaa cgaaaaaacc acctggggag gtggtttgat cgaaggttaa
     3601 gtcagttggg gaactgctta accgtggtaa ctggctttcg cagagcacag caaccaaatc
     3661 tgtccttcca gtgtagccgg actttggcgc acacttcaag agcaaccgcg tgtttagcta
     3721 aacaaatcct ctgcgaactc ccagttacca atggctgctg ccagtggcgt tttgcgtgct
     3781 tttccgggtt ggactcaagt gaacagttac cggataaggc gcagcagtcg ggctgaacgg
     3841 ggggttcttg cttacagccc agcttggagc gaacgaccta caccgagccg agataccaga
     3901 gtgtgagcta tgagaaagcg ccacacttcc cgcaagggag aaaggcggaa caggtatccg
     3961 ggaaacggca gggttggaac aggagagcgc aagagggagc gattcatcgg aaacggtgat
     4021 gatctttaag tcctgtcggg tttcgccact cctgactgat tcatggttga gccaccggct
     4081 cccacagatg caccgaaaaa gcgtctgttt atgtgaactc agtcaggagg gcggagccta
     4141 tggaaaaacg ccacctgcgt ggccttgccg ttttgcctca catgttagtc ccctgcttat
     4201 tcacggaatc tgtgggtaac tttgcatgtg tccgcagcgc ccgccgcagt ctcacggccg
     4261 gagcgtagcg accgagtgag cgaggaggcg caatttcccc ggcacctaca agacggacac
     4321 cggacgcgct gatgtggcat attgtcccct gattaagtct ttatattccc tgataataaa
     4381 ttaccaggat aggtgaatca tatcggtttt tcgctgaaca ttttacgtta acctatctct
     4441 gttgcttttt tctccttacg gagaaataca ggtcatcccc gcgagggcgt ggctgacctc
     4501 agggggatat cccctgctta cgctggctgg acgccaccga cgcacggtct ctccccctct
     4561 ttctggctcc gtccggctgg acgccggcca cgccagaaat tcacccggcc cccgtcaggc
     4621 tcacgcctga ccacccacaa aaatccacgg tgctggacgc acctggcgaa gcagcaaagc
     4681 tgctcctgat tttgcgggtg gtcagccgct cgctatggcg aactctgacg ggggccggaa
     4741 caccggaaaa gaccgcactg gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt
     4801 tgttcctccg gggctgcgcc ttttccgcat ggggcggaaa gaggctggcc tccgtggtga
     4861 aagctgaacg caatcaccac ggacgtgacc gccgccgcca tgccttgggg catgtccgcg
     4921 gcttgtgcag cgggggctgc acatcacgcc ggcaaaccat cgtctgaagg agagagaaca
     4981 tgtttaatac ggattcgctt gcctggaagt ggcacattcc ggagctggcc cggcagattt
     5041 atccgtgggt cagctggctg taccaggagt attacggtct ggccggggct ttcctagtta
     5101 tcggcctgtt tttcggtctg gctgaacttt gggccggacg gatgcgtttt gtggggctga
     5161 gtcataagga gcgacgggag tatgccgatc gctataagcg cgataataaa cggccagcgc
     5221 cattttcggt gctggcctcg ctggattcgc ccgggcggat ggtcagggca cgtcgacatg
     5281 tctggaacta cgcgtactgg ctgaccggcg cggccggtct ggcccttctg ggggggctgg
     5341 ttgtcgccgt gtttatcgag agctggggag aaaccgtcct gcgtccggac ctctacgcaa
     5401 acgcagatta ttacctgctg cagccggcta acctgttcgg gattgcctgg tgcgcgggcg
     5461 ggctggctgt cgggattgtg ttggcgcggc tgctggcgtt cctgctgctt gacggtcatt
     5521 ttgcggctgc tgacgaggcg gtgaacgcga aactgaatca ggagagccgg cgcagcagcc
     5581 agcggacagg agagatgacc gacgtgcgcc acctgcattt cggggagccg gtaccggtta
     5641 atgcgctggc cgatttttcc tcggagcagg cccggcagga gcaggcggtg tttctgggta
     5701 aggatgagca gggcaagccg gtgctggttc cgcgtgatac ctggcgcaag accaacattc
     5761 agatcctcgg cctgcccggg agcggcaaga gcgtgatggc caccaacgcg ctcattcgct
     5821 gcgtacgcga tttcggggac gcggtggtgt acttcgaccc caaaggggac gcatgggcgc
     5881 cgcacgtctt ccgggcgcac tgtccggact tcaccctgct ggatctgcgg ccggggaaac
     5941 cggcgcagct gaatctgttc cgcgatctcg accagtacgc gctgaaaaac ctgctgattg
     6001 ccgggcttaa cctgggcgag accggcgacg tggcggacca ctaccgcatc agcgagcaga
     6061 aagcggcgaa gctgattgcg gagcagtttc cgcagggggc aaacattcag caggtgctgg
     6121 cggcggcgta cgcgctgccg gaggccctga aaaaggacgt gaaggggttg atcactaggc
     6181 tggaaaacgt cgctgacctg agcgtcctgc agacggacag tggcatcgat gtggcgggga
     6241 ttatcaacgg cggcggctgc ctgtacgtta tcggctcgat ggacgacgag gcggtgatcc
     6301 gtgtgcaaaa aatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg gacgagttcc
     6361 gggagtggcc acacgtcagc gtgatgctcg atgaaattaa atatctgctg tcgaagtatg
     6421 tactgaacgc actggggacg ctgcgcagcc gggactgcaa tctgttgctc gcgcaccagt
     6481 cgctgggcga tttcgggcag tgcgggcagg atctgcccgc tgaatttgtg aaaaccacgg
     6541 tactggataa cacgccgatc cgctggtttt accgggcggc cagcctggag tccgcgcagt
     6601 gggcggcggg gcagaccggg gaaatcaggg tggacgtgga gcgccgccgt gccagccggg
     6661 aggcggggaa cgtggagcat atcagcgggg acagcttcat ccagaaggag gcgcggccgc
     6721 tgtttgacgt gaacaccctg caacacctgc cggacgggtt cgcggtcatg accgggctgg
     6781 gtgtggcccg gatggcgttc agcagtccgc tgcgggttga gcgccgggaa atcccgttgc
     6841 agcgtttccc ggtgctggcg aaaaccgatc ccctggcgga gttccggccc ccggccgacg
     6901 gcggcgggcc gaaagcggat gatgattttg cggggattta ctgatggcgc tggagctgta
     6961 caacgtcagc cacgcgaaac gacaggcgcg gaacgcggag aaaacccggc tgaccctgcg
     7021 ctggttgcgg gaagagctgt gttccacggc ggagctggtt gcgcggcggc tggggattgc
     7081 ggcggtgcag ccggtctacc gcttcctcga cagcctggtg gcgaaagggc tgctggtccg
     7141 gaccaaatat ccggtggacg gccgtcaggt cagcgtgtgg gggctgacgc cgcatggcgt
     7201 ggcgttctcg ttcgacgagg acgaaccgct ggcggatgtt attccgtttc agccgtcaag
     7261 ggtgtcggca gcacatctgc cgcaccggct ggccgtgcaa tccctgcgcc ttgatatgga
     7321 agccagaggg gccaccggct ggcgcattct gcaccgtctg agtcttaaag gcatgaaagt
     7381 gccggacgcc ctggcggagc tggacggtaa aacggtggcg tttgaggtgg agcgcaccgt
     7441 caagagccgt cgccggtatc aggaagtggt gtccgggtat ctgttcaacc gcaaagctaa
     7501 cggcatcgat gaaatctggt acatctgccc ggaccgggcg acgcaggtca gggtgcagcg
     7561 ggcaatcctg tcggtggatg aaatcgtgaa cccacagacc ggcgaggccc gcaaaacggc
     7621 agagctggat cgtgaacggt tgtttgcgtg ctttaaattt atgacaacgg agtgagggat
     7681 tatggcgctg tacatggtat ttattgaaga cgcggaggac cggaacggcg agcgctgcat
     7741 catgccgacc agcgagcatc tggaagatat ggtggtgctc gcctggcaga ccggggcgta
     7801 tgttttctgt tttgtcgacc gggcccagca tctggcgatg atgaaatcca tcgggatgta
     7861 tgacgagcgg ctcaacagcg acgaaaaagc cacgtacttt ttcactggta aaaatccgca
     7921 atgggaacgg aacaaaccca caggaatgaa gctgtgtgat tacctctatc gcaaggtgga
     7981 gcggttcgcc atcgagaacg gtgtgcaggt ggttgatgcc gggaagtgct caccggagca
     8041 gattaacgcg atgattgacg tggtgagtga gcggcacggg gaaagcgcgg tgaacgagaa
     8101 gctgaaagcg gagctgatgc gggaatataa cagccgctat tttaacgtgc cggtacctga
     8161 actgccggag gacaaacctg cccgccggtg gtggccgttc ggcaaaaaat aaatgccagc
     8221 accggccatg ccggtgctgt tattcctccc cgagcttatc ccgcatcacc tgccagagtg
     8281 tttccgcatc ctcgtgtagc cgctcgcaca tgtcggccgg ggtatccata tccagctcat
     8341 tcagtttatc gagcaatagc agcgactgct ccttaataaa tttagccata ttgagggctg
     8401 ttttttcctg ttttgtcatg attctttcct tccgggcatg gtgtgcgcct gtcaggtata
     8461 ctgtctcaaa aatagcgggg tgagacaatg gcgtgggtga cggtgagaca ggcgacggaa
     8521 ctgacgggta aagcccgaag ttccctgtat cgggatatgg cgaaaggccg cgtatcatac
     8581 cgtacggaag ctgatggcgg ccgggtggtg gataccagcg agcttatccg ggtgtacggt
     8641 gagttaagac aggatgagac tcacgagcgg gacgagttga gactgtcggg tgagactgaa
     8701 aaaatggcct ccgacgcgtt gatcgcggag ataaaagcac tcagggagga ggtggccggg
     8761 ctacggcagg aaatgcagtc catgagactg ctggaacaca aaccggagac agcagagacc
     8821 ccggccggaa caccgcgccg gtggtggccg tggggaaaac gctgacgcgc tgtgcttgtc
     8881 caaccggcga cggccacgca atgaattgcg tccccgtcac cggttattag agaacaatat
     8941 ggggattctg gcgctggccg accacggcca gaacccggac ttttctggtc tggtcatcaa
     9001 catcatagag gacatgctgt cccatcagac tgatccggcg aacgtcctcg ccatagcgtg
     9061 agtccacttc atagagtcgc gggaagtcgg ccagtttgtc cgccgcagcg agaacccggt
     9121 cggtaacgtt ctgcgcatcc cacagggatg aattttccgc gatatagtca aacagcgtcg
     9181 ttacatcctc gatggctccc tgcttccatt ccacgtcata cggcatcgcg cagttcctga
     9241 taacggccat gcaggcgctg gcggagttgt tccgtggtca tacccggacg cgtg
//
