LOCUS       NZ_CP101894             9294 bp    DNA     circular CON 09-OCT-2023
DEFINITION  Klebsiella pneumoniae strain 9362 plasmid pColRNAI_9362, complete
            sequence.
ACCESSION   NZ_CP101894
VERSION     NZ_CP101894.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29397004
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Arcari,G., Carattoli,A., Bruno,F., Polani,R., Capitani,V.,
            Menichincheri,G., Sacco,F. and Raponi,G.
  TITLE     Ceftazidime-avibactam resistance in Klebsiella pneumoniae Sequence
            Type 37: a decade of persistence and concealed evolution
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Arcari,G., Carattoli,A., Bruno,F., Polani,R., Capitani,V.,
            Menichincheri,G., Sacco,F. and Raponi,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2022) Molecular medicine, Sapienza University of
            Rome, Viale di Porta Tiburtina, Rome 00182, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.4.8.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 34.5x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 10/09/2023 02:57:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101894.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="9362"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColRNAI_9362"
                     /geo_loc_name="Italy: Rome"
                     /lat_lon="41.53 N 12.30 E"
                     /collection_date="2011-03-28"
                     /collected_by="Policlinico Umberto I Hospital"
     gene            join(7538..9294,1..208)
                     /locus_tag="NKY00_RS00005"
                     /old_locus_tag="NKY00_26895"
     CDS             join(7538..9294,1..208)
                     /locus_tag="NKY00_RS00005"
                     /old_locus_tag="NKY00_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            208..939
                     /locus_tag="NKY00_RS00010"
                     /old_locus_tag="NKY00_26900"
     CDS             208..939
                     /locus_tag="NKY00_RS00010"
                     /old_locus_tag="NKY00_26900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            946..1476
                     /locus_tag="NKY00_RS00015"
                     /old_locus_tag="NKY00_26905"
     CDS             946..1476
                     /locus_tag="NKY00_RS00015"
                     /old_locus_tag="NKY00_26905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1504..1683)
                     /locus_tag="NKY00_RS00020"
                     /old_locus_tag="NKY00_26910"
     CDS             complement(1504..1683)
                     /locus_tag="NKY00_RS00020"
                     /old_locus_tag="NKY00_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1752..2129
                     /locus_tag="NKY00_RS00025"
                     /old_locus_tag="NKY00_26915"
     CDS             1752..2129
                     /locus_tag="NKY00_RS00025"
                     /old_locus_tag="NKY00_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(2191..2490)
                     /locus_tag="NKY00_RS00030"
                     /old_locus_tag="NKY00_26920"
     CDS             complement(2191..2490)
                     /locus_tag="NKY00_RS00030"
                     /old_locus_tag="NKY00_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2480..2743)
                     /locus_tag="NKY00_RS00035"
                     /old_locus_tag="NKY00_26925"
     CDS             complement(2480..2743)
                     /locus_tag="NKY00_RS00035"
                     /old_locus_tag="NKY00_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(3049..3468)
                     /locus_tag="NKY00_RS00040"
                     /old_locus_tag="NKY00_26930"
     CDS             complement(3049..3468)
                     /locus_tag="NKY00_RS00040"
                     /old_locus_tag="NKY00_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3871..5556
                     /locus_tag="NKY00_RS00045"
                     /old_locus_tag="NKY00_26935"
     CDS             3871..5556
                     /locus_tag="NKY00_RS00045"
                     /old_locus_tag="NKY00_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5566..5823
                     /locus_tag="NKY00_RS00050"
                     /old_locus_tag="NKY00_26940"
     CDS             5566..5823
                     /locus_tag="NKY00_RS00050"
                     /old_locus_tag="NKY00_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            5908..6057
                     /locus_tag="NKY00_RS00055"
                     /old_locus_tag="NKY00_26945"
     CDS             5908..6057
                     /locus_tag="NKY00_RS00055"
                     /old_locus_tag="NKY00_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6161..6739)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          209..936
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 cctgcaacac ctgccggacg ggttcgcggt catgaccggg ctgggtgtgg cccggatggc
       61 gttcagcagt ccgctgcggg ttgagcgccg ggaaatcccg ttgcagcgtt tcccggtgct
      121 ggcgaaaacc gatcccctgg cggagttccg gcccccggcc gacggcggcg ggccgaaagc
      181 ggatgatgat tttgcgggga tttactgatg gcgctggagc tgtacaacgt cagccacgcg
      241 aaacgacagg cgcggaacgc ggagaaaacc cggctgaccc tgcgctggtt gcgggaagag
      301 ctgtgttcca cggcggagct ggttgcgcgg cggctgggga ttgcggcggt gcagccggtc
      361 taccgcttcc tcgacagcct ggtggcgaaa gggctgctgg tccggaccaa atatccggtg
      421 gacggccgtc aggtcagcgt gtgggggctg acgccgcatg gcgtggcgtt ctcgttcgac
      481 gaggacgaac cgctggcgga tgttattccg tttcagccgt caagggtgtc ggcagcacat
      541 ctgccgcacc ggctggccgt gcaatccctg cgccttgata tggaagccag aggggccacc
      601 ggctggcgca ttctgcaccg tctgagtctt aaaggcatga aagtgccgga cgccctggcg
      661 gagctggacg gtaaaacggt ggcgtttgag gtggagcgca ccgtcaagag ccgtcgccgg
      721 tatcaggaag tggtgtccgg gtatctgttc aaccgcaaag ctaacggcat cgatgaaatc
      781 tggtacatct gcccggaccg ggcgacgcag gtcagggtgc agcgggcaat cctgtcggtg
      841 gatgaaatcg tgaacccaca gaccggcgag gcccgcaaaa cggcagagct ggatcgtgaa
      901 cggttgtttg cgtgctttaa atttatgaca acggagtgag ggattatggc gctgtacatg
      961 gtatttattg aagacgcgga ggaccggaac ggcgagcgct gcatcatgcc gaccagcgag
     1021 catctggaag atatggtggt gctcgcctgg cagaccgggg cgtatgtttt ctgttttgtc
     1081 gaccgggccc agcatctggc gatgatgaaa tccatcggga tgtatgacga gcggctcaac
     1141 agcgacgaaa aagccacgta ctttttcact ggtaaaaatc cgcaatggga acggaacaaa
     1201 cccacaggaa tgaagctgtg tgattacctc tatcgcaagg tggagcggtt cgccatcgag
     1261 aacggtgtgc aggtggttga tgccgggaag tgctcaccgg agcagattaa cgcgatgatt
     1321 gacgtggtga gtgagcggca cggggaaagc gcggtgaacg agaagctgaa agcggagctg
     1381 atgcgggaat ataacagccg ctattttaac gtgccggtac ctgaactgcc ggaggacaaa
     1441 cctgcccgcc ggtggtggcc gttcggcaaa aaataaatgc cagcaccggc catgccggtg
     1501 ctgttattcc tccccgagct tatcccgcat cacctgccag agtgtttccg catcctcgtg
     1561 tagccgctcg cacatgtcgg ccggggtatc catatccagc tcattcagtt tatcgagcaa
     1621 tagcagcgac tgctccttaa taaatttagc catattgagg gctgtttttt cctgttttgt
     1681 catgattctt tccttccggg catggtgtgc gcctgtcagg tatactgtct caaaaatagc
     1741 ggggtgagac aatggcgtgg gtgacggtga gacaggcgac ggaactgacg ggtaaagccc
     1801 gaagttccct gtatcgggat atggcgaaag gccgcgtatc ataccgtacg gaagctgatg
     1861 gcggccgggt ggtggatacc agcgagctta tccgggtgta cggtgagtta agacaggatg
     1921 agactcacga gcgggacgag ttgagactgt cgggtgagac tgaaaaaatg gcctccgacg
     1981 cgttgatcgc ggagataaaa gcactcaggg aggaggtggc cgggctacgg caggaaatgc
     2041 agtccatgag actgctggaa cacaaaccgg agacagcaga gaccccggcc ggaacaccgc
     2101 gccggtggtg gccgtgggga aaacgctgac gcgctgtgct tgtccaaccg gcgacggcca
     2161 cgcaatgaat tgcgtccccg tcaccggtta ttagagaaca atatggggat tctggcgctg
     2221 gccgaccacg gccagaaccc ggacttttct ggtctggtca tcaacatcat agaggacatg
     2281 ctgtcccatc agactgatcc ggcgaacgtc ctcgccatag cgtgagtcca cttcatagag
     2341 tcgcgggaag tcggccagtt tgtccgccgc agcgagaacc cggtcggtaa cgttctgcgc
     2401 atcccacagg gatgaatttt ccgcgatata gtcaaacagc gtcgttacat cctcgatggc
     2461 tccctgcttc cattccacgt catacggcat cgcgcagttc ctgataacgg ccatgcaggc
     2521 gctggcggag ttgttccgtg gtcatacccg gacgcgtgtc agcacgggcg gcggtcactt
     2581 tggcgcgaac ctgatcttca tactctgcct gggccagttc ttcgttagcc agtcgctgca
     2641 acagcgcagc tttgtccggg ccaccttccg gcagtatgcg gggcaggctt tcagccagtt
     2701 cccgaagaaa gttggcgttt tcgtagtgtg tagtgttgct cattgcggag cctcccgggt
     2761 taatgcggca atatgaagga agtttagcag ggggcggtct gaaagaatat ggtcgtgtca
     2821 gtcaggctga tgaagcagaa agtcggtaaa aaagggctta cggcatccat tttacgtcaa
     2881 aacatatgcc atgcagttgc gtgcataggc atgcattagg ataaaattta tcgggcgcgt
     2941 ttccggcagt tttccggggg ggttgttgcc tgttttatcc cgtagccgcc ggaaacgccc
     3001 tgagcccgtc tgagcggtgc gcgtaatgac gcgttatggt aaatccactt actgtctgcc
     3061 ttcatagcca tcgagataaa cagctgaaat ggtgtcagcc agtagccttg attttttcgg
     3121 tgtgaggtct ttgctgcggt aatcggtacc cgcaaattgt gttgtgcctt ggagtgagtt
     3181 taaaaattcc tgactgtgaa actgctgaat gcgttgctgc acatcactcc gtgattttcc
     3241 ggtcgcttta tcctttgcgc ccatatccca gaactgccgg aaaaaaggca ccatcattcg
     3301 tgttgtgtca gctttctggg tggaaaaatt gggatcaaaa ccgtcatctg aagcatatac
     3361 gtaatgccgg gtatgctgtt ccacgctgcc ctttggagca caggcagtga gagttgctgt
     3421 cgctgttatc atggcgataa gacacagttt gctgccggaa cgtctcatga gcgtctcctt
     3481 acctttaatt cttttttctc attgagttat atatccgcag gctatcttat catcagtttc
     3541 tgaccgtgtt cagtgaccga acaacccgaa ccagaccccc cgtttctttt cctgtcccgg
     3601 agggggcttt ctggtatctc atataatgat ttcagtaaaa catactgagg ccggagaacg
     3661 gcgccgtttg tgctggtttt tgttgcatct ggaggggatt gcgcatggtg tgagtggctg
     3721 tttttttgat cattttcaca aaaacgcaaa tttacagact tgacatgaaa aacaaggcag
     3781 cgtaattttt gtactgtata taaaaccagt ggttatatgt acagtatttt attttagttt
     3841 attgttttaa aagtcaaaga ggaaaagtat atgagtggcg gagacggacg aggtccgggt
     3901 aattcaggtc tgggacacaa tggtggtcag gccagtggga atgtgaacgg tacatcaggt
     3961 aaaggtggcc cttcatcagg tggtggtacg gatccaaaca gcgggccggg ctggggtacg
     4021 acgcatacac ctaacgggga tattcataac tataacccgg gggagtttgg taacggcggg
     4081 agtaaacccg gtggtaatgg cggtaacagc ggcaatcata gcgggagctc tggtggcgga
     4141 cagtcttcgg ccaccgcgat ggccttcggt ctgcctgccc tggccactcc gggggctgaa
     4201 ggactggctt tatccgtttc cggcgatgcg ttgtcggccg ctgttgctga tgtgctggct
     4261 gccctgaaag gtccgtttaa gtttggtctg tgggggattg cgatttacgg agtgctgcct
     4321 tctgagatag caaaagatga tccgaaaatg atgtcaaaaa tcgtgacgtc attgccggca
     4381 gacacggtaa cggagacgcc ggtcagctcc ctgccgctgg accaggcgac ggtcagcgtc
     4441 actaaacgtg tggcggatat tgtgaaggac gagcggcagc atattgcggt tgtcaccggc
     4501 cggccaatga gtgttcctgt ggtggatgcg aaaccgacaa aacgtccggg ggtattcagt
     4561 gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac ctaaaggtgt tccggccgcg
     4621 aaagccccgc caaaaggcat tattgctgaa aaaggtgatt cacgtccggc tggttttacg
     4681 gccggtggta actcccgtga ggccgttatt cgtttcccga aagagagcgg acagaaaccg
     4741 gtctatgtgt cggtgacgga tgttcttact ccggcacagg taaaacagcg tctggaggaa
     4801 gaaaagcgtc gccagcaggc atgggacgct gctcacccgg aagaggggct gaaaagagag
     4861 tatgataaag cgaaagctga gctggatgct gaagataaaa atattgcgac cttaaacagt
     4921 cgcattgcat cgacagagaa ggcgatcccc ggtgcaaggg ctgctgttca ggaagccgat
     4981 aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg tgacttataa cccgcctcat
     5041 gaatatggct ccgggtggca ggatcaggtt cgctatcttg ataaggatat tcagaatcag
     5101 aatgagaaat taaaagctgc ccagacatct ttaaacgaaa tgaatgaatc cttatccagg
     5161 gataaggctg cgctttccgg ggcgatggag agccggaaac aaaaggagaa aaaagcgaag
     5221 gatgcagaaa ataaattaaa tgaggaaaag aaaaaacctc gtaagggaac taaagattac
     5281 ggccatgatt attttcctga tcccaagact gaagatatta aggggttggg agagttgaaa
     5341 gagggtaaac ctaaaacccc taaacaaggt ggtggtggta agcgggcccg atggtatggt
     5401 gataaaaagc gtaaaattta tgaatgggat tcccagcacg gtgagcttga agggtaccgc
     5461 gccagtgatg gcgaacacct cggggcattc gatccaaaaa cgggtaagca ggttaaaggg
     5521 ccggatccaa aacgaaacat taaaaaatat ctttaagagg taaatatggg gcttaaatta
     5581 catattcatt ggtttgataa gaaaaccgaa gagtttaaag gtggtgaata ctcaaaagac
     5641 ttcggtgatg atggttctgt cattgaaagt ctggggatgc ctttaaagga taatattaat
     5701 aatggttggt ttgatgttga aaaatcatgg gtttcgatat tacagccaca ctttaaaaat
     5761 gtaatcgata ttagtaaatt tgattacttt gtatcctttg tttaccggga tggtaactgg
     5821 taagcaaaag ttatcaagga tgatttgaaa tacagacttc cggtgttcag ggatgaatac
     5881 cggggttttt aaatacaggg aaggtatatg aaaaaggtaa aggttgtttt tctttttata
     5941 ttgatttttt caggctttct tctggtcgca tgtcaggcaa actatatccg ggatgttcag
     6001 ggtggaacgg tggcaccatc gtcctcctct gaactgacgg ggatcgcggt tcagtagaaa
     6061 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg
     6121 aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac
     6181 cgtggtaact ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac
     6241 tttggcgcac acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc
     6301 agttaccaat ggctgctgcc agtggcgttt tgcgtgcttt tccgggttgg actcaagtga
     6361 acagttaccg gataaggcgc agcagtcggg ctgaacgggg ggttcttgct tacagcccag
     6421 cttggagcga acgacctaca ccgagccgag ataccagagt gtgagctatg agaaagcgcc
     6481 acacttcccg caagggagaa aggcggaaca ggtatccggg aaacggcagg gttggaacag
     6541 gagagcgcaa gagggagcga ttcatcggaa acggtgatga tctttaagtc ctgtcgggtt
     6601 tcgccactcc tgactgattc atggttgagc caccggctcc cacagatgca ccgaaaaagc
     6661 gtctgtttat gtgaactcag tcaggagggc ggagcctatg gaaaaacgcc acctgcgtgg
     6721 ccttgccgtt ttgcctcaca tgttagtccc ctgcttattc acggaatctg tgggtaactt
     6781 tgcatgtgtc cgcagcgccc gccgcagtct cacggccgga gcgtagcgac cgagtgagcg
     6841 aggaggcgca atttccccgg cacctacaag acggacaccg gacgcgctga tgtggcatat
     6901 tgtcccctga ttaagtcttt atattccctg ataataaatt accaggatag gtgaatcata
     6961 tcggtttttc gctgaacatt ttacgttaac ctatctctgt tgcttttttc tccttacgga
     7021 gaaatacagg tcatccccgc gagggcgtgg ctgacctcag ggggatatcc cctgcttacg
     7081 ctggctggac gccaccgacg cacggtctct ccccctcttt ctggctccgt ccggctggac
     7141 gccggccacg ccagaaattc acccggcccc cgtcaggctc acgcctgacc acccacaaaa
     7201 atccacggtg ctggacgcac ctggcgaagc agcaaagctg ctcctgattt tgcgggtggt
     7261 cagccgctcg ctatggcgaa ctctgacggg ggccggaaca ccggaaaaga ccgcactggg
     7321 gtgccgcctt ttcctctcac acgctggggc gtgctcgttg ttcctccggg gctgcgcctt
     7381 ttccgcatgg ggcggaaaga ggctggcctc cgtggtgaaa gctgaacgca atcaccacgg
     7441 acgtgaccgc cgccgccatg ccttggggca tgtccgcggc ttgtgcagcg ggggctgcac
     7501 atcacgccgg caaaccatcg tctgaaggag agagaacatg tttaatacgg attcgcttgc
     7561 ctggaagtgg cacattccgg agctggcccg gcagatttat ccgtgggtca gctggctgta
     7621 ccaggagtat tacggtctgg ccggggcttt cctagttatc ggcctgtttt tcggtctggc
     7681 tgaactttgg gccggacgga tgcgttttgt ggggctgagt cataaggagc gacgggagta
     7741 tgccgatcgc tataagcgcg ataataaacg gccagcgcca ttttcggtgc tggcctcgct
     7801 ggattcgccc gggcggatgg tcagggcacg tcgacatgtc tggaactacg cgtactggct
     7861 gaccggcgcg gccggtctgg cccttctggg ggggctggtt gtcgccgtgt ttatcgagag
     7921 ctggggagaa accgtcctgc gtccggacct ctacgcaaac gcagattatt acctgctgca
     7981 gccggctaac ctgttcggga ttgcctggtg cgcgggcggg ctggctgtcg ggattgtgtt
     8041 ggcgcggctg ctggcgttcc tgctgcttga cggtcatttt gcggctgctg acgaggcggt
     8101 gaacgcgaaa ctgaatcagg agagccggcg cagcagccag cggacaggag agatgaccga
     8161 cgtgcgccac ctgcatttcg gggagccggt accggttaat gcgctggccg atttttcctc
     8221 ggagcaggcc cggcaggagc aggcggtgtt tctgggtaag gatgagcagg gcaagccggt
     8281 gctggttccg cgtgatacct ggcgcaagac caacattcag atcctcggcc tgcccgggag
     8341 cggcaagagc gtgatggcca ccaacgcgct cattcgctgc gtacgcgatt tcggggacgc
     8401 ggtggtgtac ttcgacccca aaggggacgc atgggcgccg cacgtcttcc gggcgcactg
     8461 tccggacttc accctgctgg atctgcggcc ggggaaaccg gcgcagctga atctgttccg
     8521 cgatctcgac cagtacgcgc tgaaaaacct gctgattgcc gggcttaacc tgggcgagac
     8581 cggcgacgtg gcggaccact accgcatcag cgagcagaaa gcggcgaagc tgattgcgga
     8641 gcagtttccg cagggggcaa acattcagca ggtgctggcg gcggcgtacg cgctgccgga
     8701 ggccctgaaa aaggacgtga aggggttgat cactaggctg gaaaacgtcg ctgacctgag
     8761 cgtcctgcag acggacagtg gcatcgatgt ggcggggatt atcaacggcg gcggctgcct
     8821 gtacgttatc ggctcgatgg acgacgaggc ggtgatccgt gtgcaaaaaa tgctgtttgc
     8881 ccgctgcgcg caaatcatca ttgcgcggga cgagttccgg gagtggccac acgtcagcgt
     8941 gatgctcgat gaaattaaat atctgctgtc gaagtatgta ctgaacgcac tggggacgct
     9001 gcgcagccgg gactgcaatc tgttgctcgc gcaccagtcg ctgggcgatt tcgggcagtg
     9061 cgggcaggat ctgcccgctg aatttgtgaa aaccacggta ctggataaca cgccgatccg
     9121 ctggttttac cgggcggcca gcctggagtc cgcgcagtgg gcggcggggc agaccgggga
     9181 aatcagggtg gacgtggagc gccgccgtgc cagccgggag gcggggaacg tggagcatat
     9241 cagcggggac agcttcatcc agaaggaggc gcggccgctg tttgacgtga acac
//
