LOCUS       NZ_CP101883             4678 bp    DNA     circular CON 20-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KPA1 plasmid p6, complete sequence.
ACCESSION   NZ_CP101883
VERSION     NZ_CP101883.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29785854
            Assembly: GCF_024505665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Genomic analysis of Klebsiella pneumoniae strain KPA1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Veterinary Microbiology, Chulalongkorn
            University, Henri-Dunant Rd, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/17/2023 13:10:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,379
            Genes (coding)                    :: 5,234
            CDSs (with protein)               :: 5,234
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 76 of 145
            Pseudo Genes (incomplete)         :: 66 of 145
            Pseudo Genes (internal stop)      :: 19 of 145
            Pseudo Genes (multiple problems)  :: 16 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101883.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPA1"
                     /host="dog"
                     /db_xref="taxon:573"
                     /plasmid="p6"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-07-18"
                     /collected_by="Small Animal Hospital, Chulalongkorn
                     University"
     gene            complement(398..799)
                     /locus_tag="NNX43_RS27435"
                     /old_locus_tag="NNX43_27435"
     CDS             complement(398..799)
                     /locus_tag="NNX43_RS27435"
                     /old_locus_tag="NNX43_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(799..990)
                     /locus_tag="NNX43_RS27440"
                     /old_locus_tag="NNX43_27440"
     CDS             complement(799..990)
                     /locus_tag="NNX43_RS27440"
                     /old_locus_tag="NNX43_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1406..1519
                     /locus_tag="NNX43_RS27540"
     CDS             1406..1519
                     /locus_tag="NNX43_RS27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_080882241.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(1723..3441)
                     /locus_tag="NNX43_RS27445"
                     /old_locus_tag="NNX43_27445"
     CDS             complement(1723..3441)
                     /locus_tag="NNX43_RS27445"
                     /old_locus_tag="NNX43_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697690.1"
                     /GO_function="GO:0019239 - deaminase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage dCTP deaminase"
                     /protein_id="WP_228262914.1"
                     /translation="MSLARKLREKRSNPIHSANEDSSGGDSINQITSRQSMDLVIGFCG
                     AVGSGIKSIVDVTEEELDDLGYDVYRIRVSELMQKLFSEWRDDIVTPFDRYHKLQDFGD
                     ALRNKYYPHILSEAVIHEIKIEKGIDTSSKKNKKAFLVDQLKHQDEIELLRMVYQHNFY
                     LLGVIRSEVERKRNLRDEGLSKSEVDIVIHHDRKSESDYGQQTAKAILDSDYFVKNNQG
                     QRAHLKAKVKRFIGLIHGLNGLTPTTYEKGMYAAHSASLQSACLSRQVGAAILDVNGNL
                     ISVGRNDVPKFNGGLYSADDGVNDHRCVYKGGKCYNEVRKLKIKSKISQILMSESACEL
                     GVKLTEDQAEQLADVIYKKTPVSSIIEYSRSIHAEMDAITSLARLGNGGFSDKVLYTTT
                     FPCHNCARHIVAVGITKVVYIEPYEKSLALELHDDAITEVNEHGKVIFESFEGVSPRRY
                     QKFFFSTDERKDSFGNAEKYSTKYKNHIDIQFIDSYLDFENRVVDIFRTNTNIDSGNHV
                     GRTDSTINLSSQPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPVPPDSK"
                     /name="anti-phage dCTP deaminase"
     gene            complement(3454..3636)
                     /locus_tag="NNX43_RS27450"
                     /old_locus_tag="NNX43_27450"
     CDS             complement(3454..3636)
                     /locus_tag="NNX43_RS27450"
                     /old_locus_tag="NNX43_27450"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_080882243.1"
                     /translation="MGKELSLNKIYDKNSRYINDSRGHELGKEKKVFDFEVEMIKKRRE
                     DAIRKIIEDSKSLKW"
                     /name="hypothetical protein"
     gene            4024..4233
                     /locus_tag="NNX43_RS27455"
                     /old_locus_tag="NNX43_27455"
     CDS             4024..4233
                     /locus_tag="NNX43_RS27455"
                     /old_locus_tag="NNX43_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_009310047.1"
                     /translation="MKSIKTFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     Typing          1112..1519
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tcgccggacg actcaccaca cgattcaccg gcgactcttc actagagtcg tacccagagt
       61 cgccgcgtct aaaaaacaac caaacgggga gtcgtgtcta gagtcgggtc gttattaact
      121 tattgttttt atagtcctga gtcgttaccg actctatgac gactcgccgg ggactctatg
      181 acgagtcgtc atagagtcgg atctatataa ataaaattta aaaaacgaaa tttcaggaaa
      241 gtaacccact gatattaaat acaaatctgc aaaatattaa ttttgcgtgg ggtgtgggta
      301 tttttagtgg tgaggagcgt tcagagatct gacgattttt gggaaggttc aggaactccc
      361 cgggatggcc ggagaggaaa actgaagcat gacggggtca ggattttttc cagaatttcc
      421 accatggcgc cggggattca gttaattttg cctggtgatt ttcgagaagg agcatggcct
      481 gtttcaggct gtcaatgtgc gaatccttgg acaaaagtaa ggtattcagg tgttcaatct
      541 cctgtttcag atgacgaatc acctgttcac tctggtcagc agaggctgta tcacgctgtt
      601 cagagacctg tccagaatgg tgttcaccct tcggttgctc aaagggacca aagacacgta
      661 aaagctctga agtatctacc atttttgcat tgcgttcacc gctacaggtg gacaggtcac
      721 ctgctttaat caacctgtgc agtgttgttc tactcttacc cgtcagtcgg gcagcctcac
      781 taatagacac tctggccatc agttaccatc ctgtaattca tccagtctcg aggacagtgt
      841 acggaaaaga tgctccgcat catcgtggag tttctcacaa aggtcggcct caacatcgag
      901 atcaagctcg ttaagcttct ccagcaggag cagagactga ttctggatga atttagccat
      961 gttcagcgct gttttctgtt gtttcgtcat aagaagcctc agagcgtcag tgctcagtgt
     1021 aagaacagtc tcttttccgc ttcctcgctc actcagtcgc tacgctcctg ccgttcggct
     1081 gcggcgagcg gcaggagcat gcgtgtaaaa gctcgagaaa accgagcggt ctcgtgatgg
     1141 ggaaaaggaa aggccgcaca gcggccgttt ttccataggc tccgcccccc tgacgagcat
     1201 cacggaatct gacgctcaaa tcagtggcgg cgaaacccga caggactata aagataccag
     1261 gcgtttcccc ctgatggctc cctcgtgcgc tctcctgttc ctgccattcg gtttaccggt
     1321 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     1381 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg actgctgcgt
     1441 cttatccggt aactatcatc ttgagtccaa cccggtaaga catgcaaaag caccactggc
     1501 agcagccacc ggtaactgat ttagaggagt tagtcttgaa gttatgcgcc agttaaggct
     1561 aaactgaaag aacaagtttt ggtgactgcg ctcctctaag ccagttacct tggttcatag
     1621 agccgcaaac tctagaacct taaaaaaacc gccccgcaaa ggcggttttt ttctttctcg
     1681 tctcaaggtt aagctctgta taacatcaga gtcaacagat cttcatttcg agtctggtgg
     1741 tactggtggt actggtggta ctggtggtac tggcggtacg ggcggtacgg gcggtacggg
     1801 cggtacgggc ggtacgggcg gtacgggcgg tacgggcggt acgggcggta cgggctggga
     1861 agataaatta atagtcgaat cagttctccc gacatgattg ccagaatcaa tatttgtgtt
     1921 agttctaaaa atatcaacga ctcgattttc gaaatctaaa tagctatcaa taaactgtat
     1981 atcaatgtga tttttatatt tagtggagta cttttctgcg ttaccgaaac tatctttcct
     2041 ttcatctgta gaaaagaaga atttttgata cctacgaggt gatacacctt caaaggattc
     2101 aaatataacc ttcccatgct cattcacctc ggttatagca tcatcatgca attctaaagc
     2161 gaggcttttt tcatatggct ctatataaac aacttttgtt attccaacag cgacaatatg
     2221 tctcgcgcag ttatgacaag gaaatgtggt tgtatataaa actttatcag aaaaacctcc
     2281 attacctagt ctagctaatg atgtaatagc atccatctct gcatgaattg agcgagagta
     2341 ctctataata gaagatactg gtgttttttt atatattaca tcagccagtt gctctgcttg
     2401 atcttctgtt aatttaacac caagttcaca agcgctttcc gacataagta tttgtgaaat
     2461 tttgctttta atctttagtt ttctaacttc attataacat ttaccacctt tgtatacaca
     2521 acgatgatca ttgacaccat catccgcact ataaagccca ccattgaatt taggtacatc
     2581 attacgccca acggaaatca gattcccatt aacatccaat atagcagcac caacttgacg
     2641 agaaaggcat gcagattgca gagatgctga atgagctgca tacatacctt tttcatacgt
     2701 ggttggagtt aaaccattta gcccatgtat caagccaata aaacgcttaa ccttagcctt
     2761 aaggtgcgcc ctttgtcctt gattattctt aacaaaataa tcagagtcaa gtatagcttt
     2821 cgcagtctgc tgaccataat cgctctctga ttttctatcg tgatgaataa ctatatcgac
     2881 ttctgactta ctcagccctt catctctaag attacgcttt ctttctactt cacttctaat
     2941 tacacctagt aaataaaaat tatgttggta gaccatcctt aaaagttcaa tttcatcctg
     3001 atgcttcaac tgatcgacca aaaaagcctt tttgtttttt ttacttgagg tgtcaatgcc
     3061 tttttctatt tttatttcat ggattacagc ttcagatagt atatgtgggt aatatttatt
     3121 tcttaacgca tcaccaaaat cttgcagctt atgatatcta tcaaacggag taacaatatc
     3181 atcacgccat tctgaaaaaa gtttttgcat aagctcgcta actcttatgc gataaacatc
     3241 atatcctaga tcatctagtt cttcttcagt cacatcaaca attgacttta ttcctgagcc
     3301 aacagcaccg caaaatccta taacaagatc catagactgt ctggaggtta tttggtttat
     3361 gctatcccct ccagaggagt cctcgttagc actatgaatc ggattacttc ttttttctct
     3421 taactttcta gccaggctca taaaagacct cacttaccat tttaatgatt ttgaatcctc
     3481 aattatcttc cttatagcgt cctctcttct ttttttaatc atttcaactt caaaatcgaa
     3541 aactttcttt tcctttccca gctcatgccc tctagaatca ttaatatatc tagaattctt
     3601 atcataaatt ttatttagag atagctcttt acccatcaca ccctcctgca agacgcatgg
     3661 attttactgt tatctttata acaggcaata gccaagaaac gtaatcgata gagatttttt
     3721 tttcttatcc taaacattag gaaattcggc aagagaaaag caagagagat tacgccaaac
     3781 taaccattat ttttcaattt gttaaaataa aatcatcaat gttaacattt ctatccacca
     3841 cctcatttgg tacctaaaat ccagagataa acctctggtc ttgatgcgct caattcttgt
     3901 tctttttttt tgaatgatat ccgcaaattg tttcgatttt aatcaactaa tggtcgtcct
     3961 atcatttatc acatcagggc aacaacgccc catacagaac aaaatcactt aaggaatacg
     4021 accatgaaat ccatcaaaac tttcgttgca gttgccgctc tatccatgat gtctttcggt
     4081 gcttttgctc agagcgtcag cgctaccgcc tccaccctgg atcgtgctga agcaaaaatt
     4141 gccgcacagg ccgctgaaca aggtgcgtca tacaaaatca ccagcgctca gttcaataac
     4201 cgtgtccaca tgacggctga actgaccaaa taagatggtc gcaaagggag ctgccgaaca
     4261 ggccgccggg aggcggcccg tttcgtccct tagcaggaaa accttcaggc cccttctggg
     4321 gcttttttgc atctgttatt taacataaag gcatgattgt acgcaccgct aaaacggcct
     4381 cagggcgttt ctgacggaaa accggtaaaa caggcaacaa accacccgaa aacccgccag
     4441 aaacgcgcag gctctgtttt tacggcatgc atgagtatgc acgcaagtgc atagcgcgaa
     4501 aattcacgcc attcagccag agtaagtcct gtttccggcg ttctttccat ccgaaaacgg
     4561 tgaacgggga cgcaatttat tgcgtggccg tgaagcgttg ccggcgcagc cggaaaaccg
     4621 gttttcagca cgttcaggaa tcgggttcgt cgacggccgg caatcacgtt atggcaag
//
