LOCUS       NZ_CP101882             5251 bp    DNA     circular CON 20-AUG-2023
DEFINITION  Klebsiella pneumoniae strain KPA1 plasmid p5, complete sequence.
ACCESSION   NZ_CP101882
VERSION     NZ_CP101882.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29785854
            Assembly: GCF_024505665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Genomic analysis of Klebsiella pneumoniae strain KPA1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Pachanon,R., Khine,N.O., Phumthanakorn,N., Wongsurawat,T. and
            Prapasarakul,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Veterinary Microbiology, Chulalongkorn
            University, Henri-Dunant Rd, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-JUN-2022
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/17/2023 13:10:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,379
            Genes (coding)                    :: 5,234
            CDSs (with protein)               :: 5,234
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 76 of 145
            Pseudo Genes (incomplete)         :: 66 of 145
            Pseudo Genes (internal stop)      :: 19 of 145
            Pseudo Genes (multiple problems)  :: 16 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101882.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPA1"
                     /host="dog"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="Thailand: Bangkok"
                     /lat_lon="13.736717 N 100.523186 E"
                     /collection_date="2020-07-18"
                     /collected_by="Small Animal Hospital, Chulalongkorn
                     University"
     gene            complement(16..228)
                     /locus_tag="NNX43_RS27405"
                     /old_locus_tag="NNX43_27405"
     CDS             complement(16..228)
                     /locus_tag="NNX43_RS27405"
                     /old_locus_tag="NNX43_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(232..717)
                     /locus_tag="NNX43_RS27410"
                     /old_locus_tag="NNX43_27410"
     CDS             complement(232..717)
                     /locus_tag="NNX43_RS27410"
                     /old_locus_tag="NNX43_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_013631087.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<755..1432)
                     /locus_tag="NNX43_RS27530"
                     /pseudo=""
     CDS             complement(<755..1432)
                     /locus_tag="NNX43_RS27530"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1395..1715)
                     /locus_tag="NNX43_RS27420"
                     /old_locus_tag="NNX43_27420"
     CDS             complement(1395..1715)
                     /locus_tag="NNX43_RS27420"
                     /old_locus_tag="NNX43_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_014386475.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGDD
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2352..2513
                     /locus_tag="NNX43_RS27535"
     CDS             2352..2513
                     /locus_tag="NNX43_RS27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144844.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHGSSPLAAAIGNGLEEVSLE
                     VLRQLRLN"
                     /name="hypothetical protein"
     gene            complement(2666..3286)
                     /locus_tag="NNX43_RS27425"
                     /old_locus_tag="NNX43_27425"
     CDS             complement(2666..3286)
                     /locus_tag="NNX43_RS27425"
                     /old_locus_tag="NNX43_27425"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_023312615.1"
                     /translation="MSKKHGNNKKVPQVMYIYCEGEKTEPLYLEAYISDRSKRTLSVFK
                     IPKTRKNTPEQLVDEAIKKKNSSSSAEGDVFWVVYDQEHLTPSSISCHQKAWNKANRHG
                     INIAISCVCFELWLLLHFGFTTKDFSSYDNLMSSSPLKKLITNYDKGSKLTYLAIRERI
                     GTAKDNARKLDIYAKTNYKNTHEPYLFPCYTSFHELLKAIDDF"
                     /name="RloB family protein"
     gene            complement(3279..4634)
                     /locus_tag="NNX43_RS27430"
                     /old_locus_tag="NNX43_27430"
     CDS             complement(3279..4634)
                     /locus_tag="NNX43_RS27430"
                     /old_locus_tag="NNX43_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498067.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_014386473.1"
                     /translation="MFVYLTIENFRSVKEKSTLNLSASGSSNHLTTHIYKNDGMNVGTL
                     MSAGIYGANASGKSNVLMAFEAIKFIATESGDLKEGKRIPCYEPYALSSATNEAPVKFE
                     AEFYSVEGNRFSYEVAFIKNRILFESLDYYPSRVKANLFTRDESDTWETIKFGGHYKGG
                     IKKIPFFPNNSYLAKAGNNAASPDIIKEAYNFFRKGIRHIGLNEKIRISSFGEREDVVN
                     ETAKVLCMVDTGVSGISVREVEAEIPIKMDEEIPQEIKEIIEEDYKYRYMFSHPTDEGG
                     SVSFPMGRESEGTQKLFEIIPLIRSAFNDSMVVIVDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLVFSTHNMQLMAPEKMRRDQIWFCEKNKGASSLYSLDDFDKKKIKTTTPYAAW
                     YDEGRFGGVPDINYLKVASFISGDISLVMPDIDVKELSDGFFEEFDGDLSDE"
                     /name="ATP-binding protein"
     Typing          complement(1830..2002)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.659"
                     /coverage="100.0"
                     /evalue="9.74e-41"
     Typing          1949..2462
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.617"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..1405)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.94"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttcatgccg ctgcactact ggctcaaacg ctgtaactgc ctgctcaagt tcgccacctg
       61 ctgactcaag cgcgtgacgc gctcgttcag cgccgcgttc tcccgttgca taagaccaga
      121 catcttctgc cattccgcga aggcgctctc ccactcgtcc agcctttgcg agtagtcctg
      181 atgtagctgc tctaatgcgg tcagcaactg tttttccagt tccgtcatgg gctatttcct
      241 ctccagtacc atccagttcc cttcctcccc gtaagctccc gctttcgggt tcactttgac
      301 gcaggacagt ttcttctccc cgcagagcga cagctgcacg ccgtggttct tctccgacag
      361 catggcctgc gtccgctcct gcgcccggat agtctggtaa ttcccggtga tctgctgccc
      421 ctgccaccac agaatgctcc cgctcgtggc gatcagcagt accgagacca gggtgatggt
      481 cagccacgtc cggccgacca tacgcagcac gctcagccgg ttcgactgca tcgccgcggt
      541 cataccctgc tcgtgggcgc tgatggcgtc gctgattctc ttcgcgcttg cgctcagttc
      601 cgcttttaca gactgctcgt gctcgctgaa tgcggctttc agcatctcgc cggtactctg
      661 ctgctgcgct ttcgattgct gctccaagtc cttcgccagc gctaaaagac tgctcataaa
      721 tggctccctt cagtcggatg tttcgccccc cgtccgggtc ggcaatgctg atactgtttt
      781 ttgtggtgcg gaccacctca aaacctgccg ctgtgagcgc ccctgtgacg tcctcgcgcg
      841 ttttgagctc cccggatgag gctaaggcca gcaacccacg tgtaatcgcc tgagcggctt
      901 ccagcttcgt ttccggcagt gcggacggcg tgaccagtaa ccgtcggttc tccggggcgt
      961 ttggatcatg cagccccagc ctgccgttca ccacggtctg ccaggcatcg atgcgcggcc
     1021 ggtcggcgcg atcgtagtac ggctgcaggc gtttcccggt cagcagttca gtgttgggga
     1081 tcaggaaatt cagctccagc cgccccttgt cggtgtgctc cacccacaga atgctgtact
     1141 ggtctttatc gagcccgggc atcagaaccc gctcaaagct ggccatgatc tgctcccgct
     1201 gcccgggcgg cagttcagcc tccgcgaacg acagcacgcc ggaggtgtac tttttcgcat
     1261 acggcgaggc atcgataagc tcccggactt cctccggttt tccctgcagc acggtcgccc
     1321 cctcgcgctg ccggtctttt cccagcaggt aatccaccgg accggcacca ccaccgcgcc
     1381 cccgggggtg aaatttaacg atcatcatcc cccccttttt ccagcaccgc atgccgcagc
     1441 cgctccagtc ccgcgtcgat tgccatcagt gcggccacaa tctgcacccg gtcatgaccg
     1501 gcaccaccgg cattcacttt ccgggcgatc tggttcaggt tgttccccat accggcaagc
     1561 tggcgcagca gcgccggcga tatcgacggc agtctgccgg cacgcgccgg cttctcatcg
     1621 aggcaggtct gacgcatcca cgccgccagc tgcttgccgt cgcaccgctc gagcagccgc
     1681 cggtgttcgt cttccgtcac ccacatcgtg agcatcttgt tgcgtttgtc tgccagagta
     1741 gcctcctgat aacaccggga cgggcgggaa atgtttcccg gtcggcacgg tgttggacaa
     1801 gccgcaggcg cgtcagtcgc ttttagcggg tttcggggcg cagccctgaa ccagtcacgt
     1861 agcgctagcg gagtgtatac tggcttatta tgttggcatg agtgcggtaa tcaggaaagt
     1921 gcttcatgtg acagaggaaa atgcagctgc gggcgtaagg cagaaaatgg aagttcagag
     1981 gatatgacgc ctcctcgctc actcagtcgc tgccgctcct gccgttcggc tgcggcgggc
     2041 gttacctgca cacggagaag agcgaaggca tatggcatga caaacggggc agaaaaagga
     2101 tgcgccgctg ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     2161 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2221 tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     2281 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     2341 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgccttc tccggtaact
     2401 atcatcttga gtccaacccg gaaagacacg gttcatcgcc actggcagca gccattggta
     2461 acggattaga ggaagtgagt cttgaagttc tgcgccagtt aaggctaaac tgaaaggaca
     2521 atttttggtg actgcgctcc ctctaagcca gttacccggt acataagttg gtagctcatg
     2581 taccttccaa aaaccaccgt tggtagcggt ggtttttatt tttcatagat tacagcctgt
     2641 aataagccat ggtcaatgga ttacttcaaa agtcgtcaat cgccttaagt agctcatgga
     2701 atgatgtata acatggaaat agataaggtt cgtgcgtgtt tttataatta gttttagcgt
     2761 aaatatctag ctttcttgcg ttatctttcg cagtaccaat cctctctctt atagcaaggt
     2821 aagttagttt actaccttta tcatagttag ttattaattt ttttagcggg gagctagaca
     2881 tcaaattatc atacgacgaa aaatctttgg ttgtgaagcc gaaatgcaac aataaccata
     2941 attcaaaaca aacacaggat attgctatat ttatcccatg cctattagct ttattccatg
     3001 ccttttgatg acatgaaata gacgaaggcg tcagatgctc ttgatcataa acaacccaaa
     3061 agacgtcacc ttctgcggaa gatgatgaat tctttttctt tatcgcctca tccaccaact
     3121 gctcaggtgt atttttcctt gttttaggta ttttaaatac acttaaagtt cttttagatc
     3181 tgtctgaaat ataagcctca aggtataatg gttcggtttt ctcaccctca cagtatatat
     3241 acattacttg tgggactttc ttattatttc catgtttttt actcatcaga aagatccccg
     3301 tcaaactctt caaaaaaacc atcagacaat tctttgacgt caatatccgg cattaccaag
     3361 gatatatcac cagatataaa tgaggctacc tttaagtagt ttatgtcggg aaccccacca
     3421 aacctaccct catcatacca agctgcgtaa ggtgttgttg tttttatctt tttcttatca
     3481 aaatcatcta agctataaag agacgacgca cctttatttt tttcacaaaa ccatatctga
     3541 tctcttctca ttttctctgg agccattaac tgcatattat gggttgagaa tacaagttgt
     3601 gagccttttt tattgacatc aggatcatta aataacttaa caatcaagtc agcaatatgc
     3661 gggtgcaagc tattatctaa ctcatcaaca attacaacca tactatcatt aaacgcagat
     3721 ctaattagag gtatgatttc aaataatttt tgggtgcctt cagactctct ccccataggg
     3781 aaagaaacac tccccccttc atcagtagga tgagagaaca tataacgata tttataatct
     3841 tcttcaatta tctcctttat ttcttgaggg atttcctcat ccatttttat tggtatttcc
     3901 gcctcaacct cccgtacaga tataccggaa acaccagtgt caaccatgca taaaaccttg
     3961 gccgtttcat ttacgacgtc ctccctctcc ccaaaagaag atattcttat cttttcattt
     4021 aaacctatat gtctaattcc ctttctaaaa aagttataag cttctttaat tatgtctggt
     4081 gatgcggcat tattacctgc tttcgctaag tatgaattat tagggaagaa tggtattttt
     4141 ttaatacctc ctttataatg cccaccaaac ttgattgttt cccaggtgtc gctctcatct
     4201 ctagtaaata aatttgcttt aaccctagat ggataatagt ctaatgattc aaataaaatc
     4261 cgatttttaa taaaagcaac ttcataactg aaccgattcc cctcaacaga ataaaattca
     4321 gcctcaaact ttacaggagc ctcattcgta gccgaagaaa gagcatatgg ctcatagcag
     4381 ggaatccgtt taccttcttt caaatcaccg ctttctgttg cgataaattt tatagcttca
     4441 aaagccatga gaacgttaga tttaccagac gcattagcac cataaatccc agcactcatg
     4501 agcgttccaa cattcattcc atcattttta tagatatgag ttgttaaatg gttactagaa
     4561 ccggatgcgc tcaagtttaa tgtcgatttt tccttaaccg acctaaaatt ttcaatagtc
     4621 aaataaacaa acatgcatac ctcctaacgg ggatttttcg ccaaaaaagc gaaaaatcaa
     4681 tcataggaaa gatatcctat atgttttaat tttggaacaa catcttcctt aaaaaacgtt
     4741 gtgctattta taattacacg ctatttacca taacatctgt tgcccgcacc gctccggaca
     4801 ggttcgccac aaacggggcg gcagtcaggt aaaaccggca acaaaccacc cgatacaccg
     4861 ccccgttcgt gacagataaa ttttacccgg ctgcatggtc atgcagccag atgcatacgg
     4921 tctgatttca ggccagaatc cggatgaacg ccttaaaaac aggaaaatgc ttctccagcc
     4981 agtcccagat ctccagcgcc atgtacaggc aattcagcac cgtctcaatc acgttaaaca
     5041 gtctcataat ccctccgttg tatacgctgt gatggaaaca tcacgaccgt atttagcggg
     5101 aaatttatga gcccttcttc ttggggtttg tcactggatg cgcgcagtca gcccagcgtc
     5161 ggaccgtcat agctccgctg ccgggaacgg gtttccgcca gctctttttc atgctgtttc
     5221 tgctcgtatg cccgttcggc ggccaccgcc a
//
