LOCUS       NZ_CP101865             1597 bp    DNA     circular CON 16-AUG-2023
DEFINITION  Escherichia coli strain LP50-1 plasmid pLP50-1-1kb, complete
            sequence.
ACCESSION   NZ_CP101865
VERSION     NZ_CP101865.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29932434
            Assembly: GCF_024499265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Lu,X., Li,R. and Wang,Z.
  TITLE     Widespread prevalence of plasmid-mediated colistin resistance gene
            mcr-1 and transferability in Escherichia coli isolates from Pigeons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Lu,X., Li,R. and Wang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; QitanTech nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/16/2023 04:14:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,994
            CDSs (total)                      :: 4,877
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 88 of 221
            Pseudo Genes (incomplete)         :: 136 of 221
            Pseudo Genes (internal stop)      :: 54 of 221
            Pseudo Genes (multiple problems)  :: 54 of 221
            Pseudo Genes (short protein)      :: 2 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LP50-1"
                     /isolation_source="pigeon"
                     /db_xref="taxon:562"
                     /plasmid="pLP50-1-1kb"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.25 N 119.01 E"
                     /collection_date="2021-06"
                     /collected_by="Xiaoyu Lu"
     gene            1..441
                     /locus_tag="NPX80_RS24910"
                     /old_locus_tag="NPX80_24915"
     CDS             1..441
                     /locus_tag="NPX80_RS24910"
                     /old_locus_tag="NPX80_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     gene            complement(615..1154)
                     /locus_tag="NPX80_RS24915"
                     /old_locus_tag="NPX80_24920"
     CDS             complement(615..1154)
                     /locus_tag="NPX80_RS24915"
                     /old_locus_tag="NPX80_24920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /protein_id="WP_229997960.1"
                     /translation="MWFPVLFRFLAHSLTTFGRWGCGERCHLSHIACDSVVVLSGMSRQ
                     IALQTRSEALRRTKRVLNARQGMEGAKNAYRGSQEVRRADTPNQGLSGRQEPRQPPAKP
                     NCTEQAVTQLVGGRRPLILQQWGSGVLPRRFPVALKALPKPWQTMAKIRLSAFLALAAN
                     TNHPMVGLCQKRAEVF"
                     /name="rep protein"
     Typing          complement(825..1075)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.254"
                     /coverage="96.0"
                     /evalue="1.43e-100"
ORIGIN
        1 atgagtaagg atgtcgagag aatcactcag ataaatgacc agggtgagat tatcggcggc
       61 ttcgttgccg ttatccgccc taaacaaaaa tcagcatttc agaggcattt caccatgaat
      121 caagacgcat tacggatctt ggctaaggaa ttaaccgggg agcaattcaa ggtcttaatg
      181 ctgatgcttg cagatctgga ttacgaaaac ttcattcaga tcgcccaagc agatattgct
      241 gaaactttag ggatgcagaa aacgaatgtt agtcgcgccg ttcgcgcgct actggatgtt
      301 ggggtgatct ttgagggacc taaagttggc agatcaaaaa cataccggct gaatgaacaa
      361 tttggttgga aaggcaccgt aaccaaccac aaaaaggcac tcaaaaacgg ctttagtgtc
      421 attcagggcg gccgaattta aagagccagc ggcaggcgga caatcagggg ctacgtgtta
      481 acgttttgac catgattgtc tatcctgcat tgctcttttg ccgcctcaaa atcccttgca
      541 ttttttgcac ccccgttctc cagaactacg caattcgaac aggtcctgtg actagacgtt
      601 gatcgggcaa tacgctaaaa cacttccgcg cgtttctggc ataaaccaac catgggatgg
      661 tttgtgttag cagcaagcgc cagaaacgcg ctcaatctaa tcttcgccat cgtttgccac
      721 ggtttcggca gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc agacccccat
      781 tgctgtaata tcaacggtct acgaccacct accagctgag tgactgcctg ttccgtgcag
      841 ttgggctttg cagggggctg gcgcggttcc tggcgacctg aaagcccctg gttaggggta
      901 tcggcgcgcc tgacctcctg agagcctctg taagcgtttt tcgcgccttc catgccctga
      961 cgggcattta aaacccgttt cgtgcgtctg agagcttctg agcgcgtttg aagggctatc
     1021 tgacgactca tccccgaaag aacaacaacg gaatcacagg caatatgcga gaggtgacac
     1081 cgctcgccgc agccccagcg accgaacgta gtgagcgagt gggcgaggaa gcggaagaga
     1141 accggaaacc acattgagca cgtacgcact gatgcggggt gtcggggtga agccctgacc
     1201 agatggcaaa cggaatatcg tcgcgggtga cggtattaca tttgcacatc ctgtcccgat
     1261 ttctgcggag tttaacggtg aacggacggc aaaaaacgtg caggagctgt tgaacgtgaa
     1321 aaccacgaaa tctggcaaaa atcacagctg acgaggcttg cagaggagta agccagtata
     1381 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccca aagagcagct
     1441 aggataaata tctaatggaa tcaatgcgtt agatgcctaa tataaagata cagaaaggag
     1501 aataaaaaca ttaataaaat ctaagggtta cagttgacat agttatcaat aatgataaca
     1561 ctgttatcat tattgataac tcaaataggg ttcaaag
//
