LOCUS       NZ_CP101864             3898 bp    DNA     circular CON 16-AUG-2023
DEFINITION  Escherichia coli strain LP50-1 plasmid pLP50-1-3kb, complete
            sequence.
ACCESSION   NZ_CP101864
VERSION     NZ_CP101864.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29932434
            Assembly: GCF_024499265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Lu,X., Li,R. and Wang,Z.
  TITLE     Widespread prevalence of plasmid-mediated colistin resistance gene
            mcr-1 and transferability in Escherichia coli isolates from Pigeons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Lu,X., Li,R. and Wang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; QitanTech nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/16/2023 04:14:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,994
            CDSs (total)                      :: 4,877
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 88 of 221
            Pseudo Genes (incomplete)         :: 136 of 221
            Pseudo Genes (internal stop)      :: 54 of 221
            Pseudo Genes (multiple problems)  :: 54 of 221
            Pseudo Genes (short protein)      :: 2 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LP50-1"
                     /isolation_source="pigeon"
                     /db_xref="taxon:562"
                     /plasmid="pLP50-1-3kb"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.25 N 119.01 E"
                     /collection_date="2021-06"
                     /collected_by="Xiaoyu Lu"
     gene            complement(3..398)
                     /locus_tag="NPX80_RS24890"
                     /old_locus_tag="NPX80_24895"
     CDS             complement(3..398)
                     /locus_tag="NPX80_RS24890"
                     /old_locus_tag="NPX80_24895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222910.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            578..1531
                     /locus_tag="NPX80_RS24895"
                     /old_locus_tag="NPX80_24900"
     CDS             578..1531
                     /locus_tag="NPX80_RS24895"
                     /old_locus_tag="NPX80_24900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001360358.1"
                     /translation="MATYHLNVKFGGKGQAGNHADYIERKGEYEKRKDLEYSEHGNMPG
                     WARDNPSHFWQAADQFERANGSTYREIEIALPRELTPDQRIELVQDFVRQVAGERHAYS
                     FAIHNPKASIDGGEQPHAHIMMSQRINDGMERSPEQYFRRYNAKNPERGGAKKDSGNLT
                     PTQQKEQLRTLRASWSALQNEHLARHGHRVRVDHRSLEERGIKRTPERHFGFAASSRLD
                     HAQRREILQNRAQPLEHFLEKAHSVRHQFDAQVQQKELVKTLLQDVARERGHALTARIN
                     KTGEVEFERAPTRTRSNTRGHGMGIRDNSRGYDYER"
                     /name="MobA/MobL family protein"
     gene            1543..2073
                     /locus_tag="NPX80_RS24900"
                     /old_locus_tag="NPX80_24905"
     CDS             1543..2073
                     /locus_tag="NPX80_RS24900"
                     /old_locus_tag="NPX80_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168766.1"
                     /translation="MASTEEGLLTLLLSVEHLKNTAIEQQKAVADTLAVARETLRGYRD
                     ISDSVVKQVSDEVRKGVQKTDIAGLMGERMTQDFQRLEETVAKMRANAEALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     gene            2507..3538
                     /locus_tag="NPX80_RS24905"
                     /old_locus_tag="NPX80_24910"
     CDS             2507..3538
                     /locus_tag="NPX80_RS24905"
                     /old_locus_tag="NPX80_24910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_047611220.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSKNQADKGKVG
                     GKNSGVVRWTANDDKRRRALDMYILGASTEDIAVAVGVSRWTVRRWMDSSVEWLLSKQI
                     IKI"
                     /name="replication initiation protein"
     Typing          2508..3283
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.683"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          579..1528
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.685"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattaatgat gatctgggct tgtttcaaga tcgagcctga ttgccttaag tttctgtaat
       61 atattgtctt ttgggtatct cagttcgcct gtttttgtgt ccactagtcc ggcctcgatc
      121 agtaaccccg ccgcaagaaa caggcggtga ttgcgttccc gtcgttgtgt tttcccctcc
      181 atccgttttt ggttggcctg tttgcgagcg ttctcctgtg cctgtagctc gtttttttct
      241 gcggttgcca ggacgtggag tagtttgcgc tcctgttctg ttagtccggc ttcgttatcg
      301 agcaggtcaa tgatctgcct ctgtcgtgcg gtctgttctc cgagcttcct gataaagccg
      361 attcgtttct tcaaccagtc gatgttgctg atgcgcatgt ttgggatgct ccctggtgtt
      421 ttttgcggtg tgttggggtt gtgcagtata tcgcttgcgg tgggtgggtg caggggggta
      481 tcttccgtgc taattcgtaa aattagcagg aagcgcactt atatgtttct tcgccgttgg
      541 ctcagaaaca tgtgcgctcc tgcggagggc taacaccgtg gcaacctatc acctcaatgt
      601 aaaatttggc ggcaagggac aggccggaaa ccatgccgat tacatcgaac gcaaaggcga
      661 gtacgagaaa cgaaaagatc tggaatactc agagcacggg aatatgccag gctgggcgcg
      721 agataatccc tcccatttct ggcaagcggc tgatcagttt gaacgtgcga atggttcgac
      781 ttatcgggag atcgaaatcg ccttgccgcg tgaactgaca ccggatcagc gcattgaact
      841 ggtgcaggat tttgtgcgtc aggtagctgg agaacggcac gcctatagct ttgccattca
      901 caatccaaaa gcgagcattg acggagggga acagcctcat gcgcatatca tgatgagcca
      961 gcgtattaac gatggtatgg aacgttcgcc ggaacagtat ttccggcgtt ataacgccaa
     1021 gaatcccgaa cggggaggcg caaaaaaaga ttcaggcaat ctgacaccga ctcagcaaaa
     1081 agagcaattg cggacgttac gggcgagctg gtcagcgtta cagaacgagc atttagcgcg
     1141 tcatggtcac agggtgcgcg ttgaccatcg ctcactggaa gaacgcggaa ttaaacgcac
     1201 accagaacgg catttcggat ttgccgcctc atcacggctt gatcatgcgc agcgccgcga
     1261 aatcctgcaa aaccgagcgc agccgctcga acattttcta gagaaagcgc attcggttcg
     1321 tcatcaattt gatgcacaag ttcagcaaaa agagttagtt aaaacgcttt tacaggacgt
     1381 cgcacgggag cgagggcacg cgcttacggc aagaataaat aaaacaggtg aagttgaatt
     1441 tgaacgtgcg ccaacgcgga cgcggagtaa cacgcgaggg cacggcatgg gcattcgtga
     1501 caactcacgc gggtacgact acgaacgtta aaaggggact gtatggcgag tacagaagag
     1561 gggttgctca cgctcttgtt gtccgttgag catttgaaaa acactgcgat agaacaacag
     1621 aaagcggttg cagacacgct ggcggtcgca agggaaacac tgcgggggta tcgggatatt
     1681 tctgacagtg ttgtaaaaca ggttagcgat gaagtcagaa aaggggtaca gaaaacggac
     1741 attgcggggc tgatgggtga gcgcatgacc caggattttc agcgtcttga agagactgtg
     1801 gcaaagatga gagccaacgc ggaggcgctt aacaaggaca ttgcgacaat tcatcacaat
     1861 gttgttaaag aatacaacac gctcaataaa tacagtctga aatggtttat cggtgttacg
     1921 gtcagttttc tggtcatgtt ctcgttgctt ggctggttta ccaaaacgtt gatcgacagg
     1981 aactacaccg cgacggaggt cgtgtatcag aaaattgttg atattgaaaa gaaactggac
     2041 aaaccagcaa cagggaagag ccggaaaaac tgacgcgctt cgcttgtcca acgcctcacg
     2101 ggacgcgaaa aaattccgcg tcccgttcac cgttttctga tgagaaagtt tgctgttttg
     2161 acccgttcgc ccttctgaac gctcgaaaca gatcgcgcat tgctttcgtg aatagttatg
     2221 caggctcatg aaaacgattc tgacgcgttt ttctggcttt gcctggtgtt tccttgtctt
     2281 tttgcgtttt tttgcgccag aaagcgattg agggcgtttt aaggggtacg tacaacagga
     2341 gttatggtaa atggatcggg tttgcgggaa gactcgacag gttttgtcgt tgggggtagt
     2401 gtaagcgact gaaaaacaaa cgcccctgaa attcgtgtct atccggcaag actgtcacga
     2461 agatacaggg gcgtgtgggt cgtaacacac ggaggaatcc taacacatga gcgccgcgct
     2521 tcaatacttc gaagaaaatt tacctcaccg tccctatcac acggatgatc tcgctttcgg
     2581 ccttcgcatc tccggcaaag ggcgtgcgct tcttgcgcgg tacatccagc agaaccagcc
     2641 acatgcgcag ttctggctgg tttttgacgt tgaccgcgag ggggcagcga ttgactggag
     2701 cgaccggaac gcccccgcgc cgaacatcac cgttaaaaat cctgtgaacg gacacgctca
     2761 cctgctctat gcactcaata tcgccgtgag aaccgcgcct gatgcgtcgg ttaaggcgct
     2821 gaaatatgcc gcagcggttg aacgttcgct gtgtgagaaa ctttgtgcgg atgtgaacta
     2881 cagcggcttg atttgcaaaa atccgttcca cctggaatgg ctggtgatgg agtggcgcga
     2941 ggaagcctat accctcgatg aactggctga ttatctcgat ttgagcgcct cagcgcgtcg
     3001 tagcatcgat aaacattatg ggatggggcg aaactgccac ctgttcgaaa tgacgcgcaa
     3061 atgggcttac agggcgattc gtcagggctg gccagcattc tcacagtggc ttgatgccgt
     3121 gatccagcgt gtcgaaatgt ataacgcatc gcttcccgtt ccgctttcac cggctgaatg
     3181 tcgggctatt ggcaagagca ttgcgaaata cacgcacagg aacttcacgc cggaaacttt
     3241 cgcacagtat gtggctgata cgcacacgcc agaaattcag gccgcacgag gtcgcaaggg
     3301 cgggaaggcc aattcgtcaa aaaatcaggc ggacaaaggg aaagttggcg ggaaaaacag
     3361 cggtgttgtc cggtggacgg ctaacgatga taaacgtcgt agggcgcttg atatgtacat
     3421 cttgggagcg tcaacagaag atattgcagt ggcggttggt gttagccgtt ggactgttcg
     3481 acgctggatg gatagctctg tagaatggct actgagtaaa caaataatca aaatttaatt
     3541 aacaaaaaga atacataccc ccccctgttt tgttagtttt ttgttaattt tgggtgcacg
     3601 aagcaagcct atatcagata acagcgcctg aacgggggtt tggtgttttt ttgcgcggcg
     3661 tctccgaccc gctcccacac ccccaaacca aataccgaaa ccctgcaaac caagcgagtg
     3721 gtggctccgc tcgccgcagc cctagcgacc gagccgtcag gctcgcgagt gggcgaggaa
     3781 gcggaagata atcggaagtc acattgagca cttacgcacc tatgccagat ccgcgcgtag
     3841 cgcgtcgcga aatcgaaaac attgaacacc ggcaggcaaa cgcaccacca cgcagcgg
//
