LOCUS       NZ_CP101862             7983 bp    DNA     circular CON 16-AUG-2023
DEFINITION  Escherichia coli strain LP50-1 plasmid pLP50-1-8kb, complete
            sequence.
ACCESSION   NZ_CP101862
VERSION     NZ_CP101862.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29932434
            Assembly: GCF_024499265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Lu,X., Li,R. and Wang,Z.
  TITLE     Widespread prevalence of plasmid-mediated colistin resistance gene
            mcr-1 and transferability in Escherichia coli isolates from Pigeons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Lu,X., Li,R. and Wang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; QitanTech nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/16/2023 04:14:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,994
            CDSs (total)                      :: 4,877
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 88 of 221
            Pseudo Genes (incomplete)         :: 136 of 221
            Pseudo Genes (internal stop)      :: 54 of 221
            Pseudo Genes (multiple problems)  :: 54 of 221
            Pseudo Genes (short protein)      :: 2 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101862.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LP50-1"
                     /isolation_source="pigeon"
                     /db_xref="taxon:562"
                     /plasmid="pLP50-1-8kb"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.25 N 119.01 E"
                     /collection_date="2021-06"
                     /collected_by="Xiaoyu Lu"
     gene            247..384
                     /locus_tag="NPX80_RS24815"
                     /old_locus_tag="NPX80_24820"
     CDS             247..384
                     /locus_tag="NPX80_RS24815"
                     /old_locus_tag="NPX80_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001519492.1"
                     /translation="MRKRFFVGILAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(1409..1600)
                     /locus_tag="NPX80_RS24820"
                     /old_locus_tag="NPX80_24825"
     CDS             complement(1409..1600)
                     /locus_tag="NPX80_RS24820"
                     /old_locus_tag="NPX80_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_096939302.1"
                     /translation="MTKQEKTTLNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENH"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1665..1988
                     /locus_tag="NPX80_RS24825"
                     /old_locus_tag="NPX80_24830"
     CDS             1665..1988
                     /locus_tag="NPX80_RS24825"
                     /old_locus_tag="NPX80_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_096939303.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVSRSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2197..>2628
                     /locus_tag="NPX80_RS24830"
                     /old_locus_tag="NPX80_24835"
                     /pseudo=""
     CDS             2197..>2628
                     /locus_tag="NPX80_RS24830"
                     /old_locus_tag="NPX80_24835"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2666..3196
                     /locus_tag="NPX80_RS24835"
                     /old_locus_tag="NPX80_24840"
     CDS             2666..3196
                     /locus_tag="NPX80_RS24835"
                     /old_locus_tag="NPX80_24840"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_073534392.1"
                     /translation="MSNLLQTGAEFEKKLKERAASTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVSTMDTHWQTVLKMSVKRWLWLIIVSVLMFTTTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTE
                     DKGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            3203..3436
                     /locus_tag="NPX80_RS24840"
                     /old_locus_tag="NPX80_24845"
     CDS             3203..3436
                     /locus_tag="NPX80_RS24840"
                     /old_locus_tag="NPX80_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_073534391.1"
                     /translation="MTELEKHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRGNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5080..5499)
                     /locus_tag="NPX80_RS24845"
                     /old_locus_tag="NPX80_24850"
     CDS             complement(5080..5499)
                     /locus_tag="NPX80_RS24845"
                     /old_locus_tag="NPX80_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5583..6020)
                     /locus_tag="NPX80_RS24850"
                     /old_locus_tag="NPX80_24855"
     CDS             complement(5583..6020)
                     /locus_tag="NPX80_RS24850"
                     /old_locus_tag="NPX80_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024177063.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTGQDGRR
                     EFETSELIRAYGELKQDETAERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            6279..7844
                     /locus_tag="NPX80_RS24855"
                     /old_locus_tag="NPX80_24860"
     CDS             6279..7844
                     /locus_tag="NPX80_RS24855"
                     /old_locus_tag="NPX80_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_072643317.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAAQAKAAVEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEAEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     Typing          1237..1324
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(668..1214)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.384"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1979..3519
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.393"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctttatgaa cttaaaaaat atgtgttcaa tagcatattt tgaatatggg catagaatag
       61 tgcttatcac tattgcgtat agcatcttat ctgacgctag gaaataatgc ccttcgttat
      121 tttttgttat aaggtatata tatataagtg tgcaatatag gccaaacaaa atgtttttta
      181 tgtagtatct taaactcata aattaaacct cgttatatat tcttttcatt ttataaggat
      241 cgagttatga ggaaaagatt ttttgtggga atattagcta taaacctcct tgttggatgt
      301 caggctaact atatacgtga tgttcaggga gggactgtgg ctccatcatc ttcttctaaa
      361 ttgacgggga tctctgttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      421 tgcgcgtaat ctgctgcctg taaacgaaaa aaccgcctgg ggaggcggtt tgatcgaagg
      481 ttaagtcagt tggggaactg cttaaccgag gtaactggct tcagcagagc gcagatacca
      541 aatactgtcc ttccagtgta gccgtagtcg ggccactact tcaagaactc tgttgcacca
      601 tttgcgccat catcgctctg ctaaccaagt taccaacggc tgctgccagt ggcgctttgt
      661 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      721 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      781 accaacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      841 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      901 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt ctgtttttgt
      961 gatgctcgtc aggggggcgg agcctatgga aaaacgcctg cggcgcggcc ttttccctgg
     1021 ttttgctttt ttgctcacat gttctttccg gctttatccc ctgattctgt ggataacagt
     1081 atcgccgctt ttgagtgagc agacaccgct cgccgcagcc gaacgaccga gcgtagcgag
     1141 tcagtgagcg aggaagcgga agagcaccgg tacctgcatt ttcgccttac gcatctgtgc
     1201 ggcatttcac accgtacatg gtgcactttt catacaatcc gcactgatgc cgcatggcta
     1261 agccagtata cactccgcta tcgctacgtg actgggtcag ggctgcgccc cgacacccgc
     1321 taaaacctgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct cacagacaag
     1381 ctgtgaccgt ctccgggagc tgcatgtgtc agtggttttc accgtcatcc ccgaagcgtg
     1441 cgaggcagct gcggtaaagc tcatcggcgt ggtcgtgaag cgattcacag atatctgcct
     1501 gttcatccgc gtccagttcg ttgagcttct ccagcagcgt taatgtctgg cctctgatga
     1561 agcgggccat gttaagggtg gttttttcct gtttagtcac tgatgcctcc atgtaagggg
     1621 gatttctgtt catgggggta atgataccga tgaaacgcga gaggatgctc acgatacggg
     1681 ttactgatga tgaacatgcc cggttactgg aacgctgtga gggtaaacaa ctggcggtat
     1741 ggatgcggcg ggtctgcctg ggtgagccgg tttcccgttc cgggaagctg cctacactgg
     1801 caccgccgtt actgcgtcag ctggccgcca tcgggaataa cctgaatcag acagcgagga
     1861 aggtgaacag cgggcagtgg tcttccggtg accgggttca ggtagtggcc gcactgatgg
     1921 ccatcgggga tgagctgcgc cggctgcgtc tggccgtcag ggaacagggg acgcgggatg
     1981 atagttaaat ttcatgccag gggaaaaggt ggtggcagtg gtccggttga ttacctgctg
     2041 ggaagggagc gtaaccggga aggggcaacg gtgcttcagg gtaatccgga agaagtccgg
     2101 gaactcatcg atgccacgcc atttgcgaag aaatacacgt caggtgttct gtcgttcgcg
     2161 gagaaggagc tgccgccggg agggcgtgaa aaagtgatgg cgagctttga gcgtgtactg
     2221 atgcccggtc tcgaaaagaa tcagtacagc atcctgtggg tagagcacca ggacaaggga
     2281 cggcttgagc tgaattttgt cattccgaac atggagttac agaccggaaa acgcctccag
     2341 ccgtactacg accgggcaga caggccgaga attgatgcct ggcagacgct ggttaatcac
     2401 cattacgggc tgcatgaccc caacgccccg gagaaccgcc ggatactgac tctccctgat
     2461 aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca cacgaggtat agatgcactt
     2521 taccatgccg gagagataaa aggtcgtcag gatgtgattc aggcgctcac ggaagcgggg
     2581 cttgaagtgg tcagagtgac gcgaagcagc atcagcattg cagacccgaa cggcgggaag
     2641 aatatcaggc tgaaaggagc attctatgag caatctttta cagacgggcg cggagttcga
     2701 gaaaaagctg aaagagagag ccgcatctac agagaaaatg ctgaacgacg agttcaggaa
     2761 gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa tctttaatca ggaacgctat
     2821 caacgaccat acaacagccc tgaaagaact tctggagagg tatcagaaaa cgaccgtcag
     2881 tacgatggac acacactggc aaaccgtgct gaaaatgagc gtgaaacgct ggctgtggct
     2941 gattatcgtc tcagtgctga tgtttaccac gaccgggagt ctgctgtggt accagggcat
     3001 gaagataaac gcaaacatga atattctccg cgagcagaaa gagagtctgg agaagctgaa
     3061 cgcgaagaca tggggcgtgc gttatcacga ggacagcaac ggacgttttc tggtgttgcc
     3121 gaaggggatg aaagccgaaa cgaactggac ggaggacaaa ggcaaactga acgcagtgag
     3181 actggtacag gagtgacaga gcatgacgga gctggaaaaa catttgctga acgcattaga
     3241 gcaactgcaa caggactata tgcagcggct gaacgaatgg gagagcgcct tcgtggaatt
     3301 gcagaaaatg ttttcgctta cgcaacgggg caacgcgatg ctgaacgagc gggtcatgca
     3361 gttgagtcag caggtgcagc acttgagcga gcggacagag cgcttgagcc agttatacag
     3421 cgagaacagg agataaggga tgaacggctg atgcaggaac gcgaacatga gttatctctg
     3481 gaacgggagc ggacgctgga tggcccttcg ctgggatggt gactgaaagt ccggttgagt
     3541 cgtaaacaga atattttcca gaaatgtcag gctgcctatt ggtaatataa ccagtcgttc
     3601 ttgcggttta ttgatttttg tacaggttgt tgctgttttt tttgtaaaga ggacagggat
     3661 ttttctgaaa atgaacttgc gggaatgcgc tgattatgta aaaaatataa ttgcgtatct
     3721 ccgatatgtt tgtaataccg atgtggacac agcgaatctg gaaggggtcg gagatgcgcg
     3781 ttctctcatg tgttaatatt gtgttcttat attgtgatgt gaatattttt tattttttct
     3841 ttgcatcttg gaaacatgca aattatattc tgatatgcaa aagtcaggac tagacatact
     3901 atccacctat tcagttctta ccttacgccc tcactaagtt cctgccgtga gggctttttt
     3961 catgggtgtg aaaaaaggaa gccccacctg acggaggggc tgagtgatac agagcaaaaa
     4021 aatatcctgg tgttggtcac ctctggagag taataagatt aaccataaag cgaactataa
     4081 acaagaatat tgatgccagg aattttctgg gttaattgtt ttgtttttta caaaaaaaga
     4141 caaaataaca gaaaataaaa tctaatcatt tttgttgctc gttgttgctc ggtgccgtta
     4201 ttcttaagct gaaagtgaat tcttctaatt ttttcttata ttcagacgca attaacagtc
     4261 cctgctcctt gagggacttt tttttatctt tttctctctg ttttatactg ttttgagtag
     4321 taactataaa ggcagtgaag ttctgtctta tgagcgcatt tctggcggtg atgcgactaa
     4381 cccattctac atcaacgaac aattcaccag aaacgcgcct gtaaattttt agctgagcaa
     4441 gggctctgtg tcgagaggtg tggatgtctg gttcaccatt ttaaaacact tcttccggcg
     4501 gtttttagtt tcgatgcagg ccagggggcg ctgtaaagtt atggtccctg attactgtgc
     4561 cagaattctg agacagacag gaggtctacc ggttattttt cgaattgggc gcttccggaa
     4621 gagagagttt cattggacgt tggtcgcgga tggtgagcac gtttcgatgt tgtgtgacga
     4681 acattcagct cagttgatgg agactgtcag aaacgcgctc agaagtgttg taacgcgatt
     4741 tctggtatca ggcaattgtt tcctttttct ggatccttat acagtgtgtg tacggtgatg
     4801 agttctgtca cgcacagtaa gattcccccc cccagatgct tccatctggc aggaattgat
     4861 ccggctgaaa atatgaaaaa acacccgttt aagtaatctc aggcaggtaa ttcgttcgtg
     4921 attcgcctgg ctgcatagat atgcataaga ttaaaattta tcaggcgcga tcgcggcggt
     4981 ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gagatcgcgc tgaacgcatt
     5041 tcagtggtgc gtacaattaa gggattatgg taaatccact tactgtcttc cctcgtagcc
     5101 atcgagataa accgcagaaa tcgtctcagc cagcagcctg gattttttcg gggtaaggtc
     5161 tttgctgcgg taatcagtac ccgcaaattg agttgtgccc cggagtgagt ttaaaaattc
     5221 ctggctgtga aactgctgaa tgcgttgctg cacatcactc cgtgattttc ctgtcgcttt
     5281 atcctttgct cccatatccc agaactgccg gaaaaaaggc accatcattc gtgttgtgtc
     5341 ggccttctgg gtggaaaagt tgggatcaaa accatcgtct gatgcataaa cgtaatgccg
     5401 ggtatgttgt tccacactgc cctttggcgt gcaggctgtc aggaccgttg ccgctgtcag
     5461 cagggcgata agatacagtg tgccgccgga acgtctcatg agtgtctcct tacctttaaa
     5521 tctttgtact cagtgactta tatttaaccg ctgactatct tatcatcact tgcagccggt
     5581 gctcaccggc tgaacagtct ggcccagaat ccccgttttt tttcctgctc aagtgcctgc
     5641 ctgagctgct ctatctcttt atgtagctgt tcccgttcgg cttcctggcg ccggcgttcc
     5701 gtatcctgtg cctgtttgtc ctcaagcatc agtgtcaggc attgtttcag ctcattcagc
     5761 tcccggagaa tgcgttctgt ctgctgatca tgtggatttt ctgcctgtcc cgcactgtgc
     5821 ctttctgctg tctcatcctg ctttaattcg ccgtatgccc ggatcagttc actggtttca
     5881 aactcccgtc gtccgtcctg gccggtacgg taactgacaa ggcctgcgcg catatcacgg
     5941 taaaactgtg aacgcgatct gcctgtcatt ttcagtgcgt cctggagtgt gtgccatgcc
     6001 atgaagtgac agtgtcccat tggtgtctca tatcatggtt tcagtaaaac atactgaggc
     6061 cggagaacgg taatgtttgt gctggttttt gtggcatcgg gtgggaatag cacgtggtgt
     6121 gggtggcttt tttttgaacg ttttcacaaa aatgaaaatc cacagggttg acagggaaaa
     6181 tgcagcggcg taattttaaa ctgtatataa aaccagtggt tatatataca gtatattgct
     6241 tttaactatt tgtttttaat gtcaaagagg attttataat ggaaacagct gtagcgtact
     6301 ataaagatgg tgttccttat gatgataagg ggcaggtaat cattactctt ttgaatggta
     6361 atccagacgg gagtggctct ggcggcggtg gtggaactgg aggtagcaaa agtgaaagtt
     6421 ctgcagccat tcatgccaca gctaaatggt ctactgctca attgaagaaa acgcaggcag
     6481 aacaggctgc ccaggcaaaa gctgccgtag aagcacaggc taaagcaaag gcaaaccggg
     6541 atgcgctgac tcaacatctg aaggatattg tgaatgaggc gcttcgtcat aattccactc
     6601 atccggaggt tattgacctg gctcatgcca ataatgcagc gatgcaggca gaagcagagc
     6661 ggttgcgtct tgcaaaagca gaagaaaaag cccgtaaaga agcggaagct gcggaaaagg
     6721 cttttcagga ggcagaacaa cgacgtaaag agatagagaa ggagcaggct gaaacagaac
     6781 gccagttgaa actggctgaa gctgaagaga aacggctggc agcattgagt gaagaggccc
     6841 gggcagtgga ggtggcacaa aaaaatcttg ctgctgcaca atctgagctg gcgaaagtgg
     6901 atgaagagat taagactctc aatacccgtt taagctccag tattcatgcc cgtgatgcag
     6961 aaatgaatac gctgtccgga aaacgaaatg agctggctca ggcatctgct aaatataagg
     7021 aacttgatga actggtcaaa aaattgtcac caagagctaa tgatccgctt cagagtcgtc
     7081 cttttttcta tgccaccagt cgacggacgg gagccggtaa gattatggag gaaaaacaaa
     7141 aacaggtaac agcatcagaa acgcgtatta accaacttaa tgctgagata aatggaattc
     7201 agggggctat gtctcaggct aataataatc gtaatacagc tgttttacgt gttcatgaat
     7261 ctgaagaaaa tttgaaaaca gcgcagacta atctcctgaa ctcgcagatt aaggatgctg
     7321 tggatgcaac agttagcttt tatcaaacgc tatctgaaaa atatggtgaa aaatattcaa
     7381 aaatggcaca ggagcttgct gataagtcta aaggtaagaa aatcagcaat gtgaatgaag
     7441 ctctagctgc ttttgaaaaa tacaaggatg ttttaaataa gaaattcagc aaagcagacc
     7501 gtgatgcgat tttcaatgca ctggaatcgg ttaagtatga agactgggct aagcatttag
     7561 atcagtttgc taagtacttg aagattacgg gacatgtttc ttttggatat gatgtggtat
     7621 ctgatatcct aaaaattaag gatacaggtg actggaagcc actatttctt acattagaga
     7681 agaaaactgt agatgctgga gttagttatg ttgttgtttt actttttagt gtgcttgctg
     7741 gaactacatt aggtatctgg gggattgcta ttgttacagg cattctatgt gcctttattg
     7801 acaagaataa acttaatact ataaatgatg tgttgggtat ttaattattc tttattgata
     7861 taaacagtcc tagcaatcca aatgggattg ctaggaccaa acaaagtaga ttgtatagca
     7921 taaataggtt taattttgcc acgggggcat tatttagatt ttttcttttt ctgaaaaaac
     7981 ctt
//
