LOCUS       NZ_CP101861            33309 bp    DNA     circular CON 16-AUG-2023
DEFINITION  Escherichia coli strain LP50-1 plasmid pLP50-1-MCR1, complete
            sequence.
ACCESSION   NZ_CP101861
VERSION     NZ_CP101861.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29932434
            Assembly: GCF_024499265.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33309)
  AUTHORS   Lu,X., Li,R. and Wang,Z.
  TITLE     Widespread prevalence of plasmid-mediated colistin resistance gene
            mcr-1 and transferability in Escherichia coli isolates from Pigeons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33309)
  AUTHORS   Lu,X., Li,R. and Wang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; QitanTech nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/16/2023 04:14:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,994
            CDSs (total)                      :: 4,877
            Genes (coding)                    :: 4,656
            CDSs (with protein)               :: 4,656
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 88 of 221
            Pseudo Genes (incomplete)         :: 136 of 221
            Pseudo Genes (internal stop)      :: 54 of 221
            Pseudo Genes (multiple problems)  :: 54 of 221
            Pseudo Genes (short protein)      :: 2 of 221
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101861.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LP50-1"
                     /isolation_source="pigeon"
                     /db_xref="taxon:562"
                     /plasmid="pLP50-1-MCR1"
                     /geo_loc_name="China: Jiangsu"
                     /lat_lon="32.25 N 119.01 E"
                     /collection_date="2021-06"
                     /collected_by="Xiaoyu Lu"
     gene            join(32621..33309,1..220)
                     /gene="virB9"
                     /locus_tag="NPX80_RS24625"
                     /old_locus_tag="NPX80_24630"
     CDS             join(32621..33309,1..220)
                     /gene="virB9"
                     /locus_tag="NPX80_RS24625"
                     /old_locus_tag="NPX80_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            213..1331
                     /gene="virB10"
                     /locus_tag="NPX80_RS24630"
                     /old_locus_tag="NPX80_24635"
     CDS             213..1331
                     /gene="virB10"
                     /locus_tag="NPX80_RS24630"
                     /old_locus_tag="NPX80_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            1333..2361
                     /gene="virB11"
                     /locus_tag="NPX80_RS24635"
                     /old_locus_tag="NPX80_24640"
     CDS             1333..2361
                     /gene="virB11"
                     /locus_tag="NPX80_RS24635"
                     /old_locus_tag="NPX80_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            2348..4210
                     /gene="taxB"
                     /locus_tag="NPX80_RS24640"
                     /old_locus_tag="NPX80_24645"
     CDS             2348..4210
                     /gene="taxB"
                     /locus_tag="NPX80_RS24640"
                     /old_locus_tag="NPX80_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            4219..4641
                     /locus_tag="NPX80_RS24645"
                     /old_locus_tag="NPX80_24650"
     CDS             4219..4641
                     /locus_tag="NPX80_RS24645"
                     /old_locus_tag="NPX80_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            4625..4930
                     /locus_tag="NPX80_RS24650"
                     /old_locus_tag="NPX80_24655"
     CDS             4625..4930
                     /locus_tag="NPX80_RS24650"
                     /old_locus_tag="NPX80_24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <5043..5237
                     /locus_tag="NPX80_RS24655"
                     /old_locus_tag="NPX80_24660"
                     /pseudo=""
     CDS             <5043..5237
                     /locus_tag="NPX80_RS24655"
                     /old_locus_tag="NPX80_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            5241..5813
                     /locus_tag="NPX80_RS24660"
                     /old_locus_tag="NPX80_24665"
     CDS             5241..5813
                     /locus_tag="NPX80_RS24660"
                     /old_locus_tag="NPX80_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            5818..7971
                     /locus_tag="NPX80_RS24665"
                     /old_locus_tag="NPX80_24670"
     CDS             5818..7971
                     /locus_tag="NPX80_RS24665"
                     /old_locus_tag="NPX80_24670"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            7968..8174
                     /gene="hha"
                     /locus_tag="NPX80_RS24670"
                     /old_locus_tag="NPX80_24675"
     CDS             7968..8174
                     /gene="hha"
                     /locus_tag="NPX80_RS24670"
                     /old_locus_tag="NPX80_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            8192..8656
                     /locus_tag="NPX80_RS24675"
                     /old_locus_tag="NPX80_24680"
     CDS             8192..8656
                     /locus_tag="NPX80_RS24675"
                     /old_locus_tag="NPX80_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            8743..9447
                     /locus_tag="NPX80_RS24680"
                     /old_locus_tag="NPX80_24685"
     CDS             8743..9447
                     /locus_tag="NPX80_RS24680"
                     /old_locus_tag="NPX80_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            10230..10325
                     /gene="dqlB"
                     /locus_tag="NPX80_RS25020"
     CDS             10230..10325
                     /gene="dqlB"
                     /locus_tag="NPX80_RS25020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            10988..11635
                     /locus_tag="NPX80_RS24685"
                     /old_locus_tag="NPX80_24690"
     CDS             10988..11635
                     /locus_tag="NPX80_RS24685"
                     /old_locus_tag="NPX80_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            11710..11979
                     /locus_tag="NPX80_RS24690"
                     /old_locus_tag="NPX80_24695"
     CDS             11710..11979
                     /locus_tag="NPX80_RS24690"
                     /old_locus_tag="NPX80_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            12140..12769
                     /locus_tag="NPX80_RS24695"
                     /old_locus_tag="NPX80_24700"
     CDS             12140..12769
                     /locus_tag="NPX80_RS24695"
                     /old_locus_tag="NPX80_24700"
                     /inference="COORDINATES: protein motif:HMM:NF022440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_223424520.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLRYKL"
                     /name="DUF2806 domain-containing protein"
     gene            12943..14568
                     /locus_tag="NPX80_RS24700"
                     /old_locus_tag="NPX80_24705"
     CDS             12943..14568
                     /locus_tag="NPX80_RS24700"
                     /old_locus_tag="NPX80_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            14640..15362
                     /locus_tag="NPX80_RS24705"
                     /old_locus_tag="NPX80_24710"
     CDS             14640..15362
                     /locus_tag="NPX80_RS24705"
                     /old_locus_tag="NPX80_24710"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_039003037.1"
                     /translation="MPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVADWFYLGMG
                     HWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKWLVPVTKFR
                     VRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRNTIPDKCFP
                     AAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSHTVVSMMLA
                     WAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(15753..16280)
                     /locus_tag="NPX80_RS24710"
                     /old_locus_tag="NPX80_24715"
     CDS             complement(15753..16280)
                     /locus_tag="NPX80_RS24710"
                     /old_locus_tag="NPX80_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(16415..16819)
                     /locus_tag="NPX80_RS24715"
                     /old_locus_tag="NPX80_24720"
     CDS             complement(16415..16819)
                     /locus_tag="NPX80_RS24715"
                     /old_locus_tag="NPX80_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(16832..17698)
                     /locus_tag="NPX80_RS24720"
                     /old_locus_tag="NPX80_24725"
     CDS             complement(16832..17698)
                     /locus_tag="NPX80_RS24720"
                     /old_locus_tag="NPX80_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            19113..19628
                     /locus_tag="NPX80_RS24725"
                     /old_locus_tag="NPX80_24730"
     CDS             19113..19628
                     /locus_tag="NPX80_RS24725"
                     /old_locus_tag="NPX80_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            19733..20275
                     /locus_tag="NPX80_RS24730"
                     /old_locus_tag="NPX80_24735"
     CDS             19733..20275
                     /locus_tag="NPX80_RS24730"
                     /old_locus_tag="NPX80_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            20272..20523
                     /locus_tag="NPX80_RS24735"
                     /old_locus_tag="NPX80_24740"
     CDS             20272..20523
                     /locus_tag="NPX80_RS24735"
                     /old_locus_tag="NPX80_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            20510..20737
                     /locus_tag="NPX80_RS24740"
                     /old_locus_tag="NPX80_24745"
     CDS             20510..20737
                     /locus_tag="NPX80_RS24740"
                     /old_locus_tag="NPX80_24745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            21191..21490
                     /locus_tag="NPX80_RS24745"
                     /old_locus_tag="NPX80_24750"
     CDS             21191..21490
                     /locus_tag="NPX80_RS24745"
                     /old_locus_tag="NPX80_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256500690.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKGFIECLC"
                     /name="hypothetical protein"
     gene            21581..21931
                     /locus_tag="NPX80_RS24750"
                     /old_locus_tag="NPX80_24755"
     CDS             21581..21931
                     /locus_tag="NPX80_RS24750"
                     /old_locus_tag="NPX80_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            22236..22484
                     /locus_tag="NPX80_RS24755"
                     /old_locus_tag="NPX80_24760"
     CDS             22236..22484
                     /locus_tag="NPX80_RS24755"
                     /old_locus_tag="NPX80_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            22481..22828
                     /locus_tag="NPX80_RS24760"
                     /old_locus_tag="NPX80_24765"
     CDS             22481..22828
                     /locus_tag="NPX80_RS24760"
                     /old_locus_tag="NPX80_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            23189..23692
                     /gene="taxA"
                     /locus_tag="NPX80_RS24765"
                     /old_locus_tag="NPX80_24770"
     CDS             23189..23692
                     /gene="taxA"
                     /locus_tag="NPX80_RS24765"
                     /old_locus_tag="NPX80_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            23696..24925
                     /gene="taxC"
                     /locus_tag="NPX80_RS24770"
                     /old_locus_tag="NPX80_24775"
     CDS             23696..24925
                     /gene="taxC"
                     /locus_tag="NPX80_RS24770"
                     /old_locus_tag="NPX80_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            25002..25295
                     /locus_tag="NPX80_RS24775"
                     /old_locus_tag="NPX80_24780"
     CDS             25002..25295
                     /locus_tag="NPX80_RS24775"
                     /old_locus_tag="NPX80_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            25292..25507
                     /locus_tag="NPX80_RS24780"
                     /old_locus_tag="NPX80_24785"
     CDS             25292..25507
                     /locus_tag="NPX80_RS24780"
                     /old_locus_tag="NPX80_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            25863..26483
                     /locus_tag="NPX80_RS24785"
                     /old_locus_tag="NPX80_24790"
     CDS             25863..26483
                     /locus_tag="NPX80_RS24785"
                     /old_locus_tag="NPX80_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            26497..26823
                     /locus_tag="NPX80_RS24790"
                     /old_locus_tag="NPX80_24795"
     CDS             26497..26823
                     /locus_tag="NPX80_RS24790"
                     /old_locus_tag="NPX80_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            26842..29589
                     /locus_tag="NPX80_RS24795"
                     /old_locus_tag="NPX80_24800"
     CDS             26842..29589
                     /locus_tag="NPX80_RS24795"
                     /old_locus_tag="NPX80_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            29601..30317
                     /locus_tag="NPX80_RS24800"
                     /old_locus_tag="NPX80_24805"
     CDS             29601..30317
                     /locus_tag="NPX80_RS24800"
                     /old_locus_tag="NPX80_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            30329..31423
                     /locus_tag="NPX80_RS24805"
                     /old_locus_tag="NPX80_24810"
     CDS             30329..31423
                     /locus_tag="NPX80_RS24805"
                     /old_locus_tag="NPX80_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            31938..32624
                     /locus_tag="NPX80_RS24810"
                     /old_locus_tag="NPX80_24815"
     CDS             31938..32624
                     /locus_tag="NPX80_RS24810"
                     /old_locus_tag="NPX80_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     ARG             12944..14565
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(16833..17704)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          23697..24922
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2349..4207
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          30330..31396
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          31939..32621
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          32622..33307
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.563"
                     /coverage="99.0"
                     /evalue="1.8e-140"
ORIGIN
        1 atttgctgta agagatggtc aggaacagac acttgctttt aatgtggaaa caaaaggcaa
       61 gtataaaatt atggttatcc acaacgttaa tgacaaattt gttttacgct atggaaacag
      121 cgttgttggt gttgtgaata aatctttcgg aaaagtatta accgaccaga ggaatacatc
      181 ttctcctgcg gtagaacgtg ttgaggtgaa taatgactga tatgaaaaaa acagatgatt
      241 tagttgatga tgcgaatcag tcaaagctgg aaagtaaacc cgttcatgat atcggtgata
      301 tcaggaacaa aaataatcgt atgcgttctg gctttctgtt tggtctgatg gctgtattag
      361 ttattggtat ttttgcgctg aaaacatata aaaattattt tgcagatgac cagcagaaag
      421 catcagaatc aacaggcgat accagcattt ctcaggtaag taaaatccgt actggtcttg
      481 gtcagaattt tgaccctgtt gagaacaaag caattatcaa tccaggcgct actggttctg
      541 gtaatactgt ttctgacggt cataaagaag tgccacagga ggggttcagg aaatatttgt
      601 caatacctgt tgctggtcag ggagggagtc agactggcgc gagtggcagc agcaggacat
      661 cgcagacatc agagccacag gaagaaagga aagaatcaaa agaaaatgtg cctggtaaat
      721 caggaatgaa agtaactgcg attaatctcg atcctgatct ctatattgag gaaaacaggc
      781 ttataccgtg tgctttaacc acacgctttg tttctgatgt tgcaggtcgt atcagttgtg
      841 tttttacaga agatgtatgg agtgctaatc accacacaaa attactggaa cagggaacta
      901 aggcttttgg tcgttaccag accggaacgc tgaatcatgg acagggcaga atgttcgtta
      961 tgtgggagca gttgcggacg cctgataata aacgaatcga tatggtaaat acagcagcag
     1021 ctggtcccct gggagaagcg ggcattgatg gatggatcga ttctcacttc tgggagcgtt
     1081 ttggtggttc tcttatgctc agtatggttc aggatgtagc ggcggcggcg gcggataatg
     1141 cacctggtaa agatcgtaat gtggattata ccgaaaactc acgacaggca atggctgaaa
     1201 tggctaaagt cgcacttgag aattcgataa atattcctcc aacaatgtat aaaaaccagg
     1261 gtgacataat cactattatg gtcggggaag acattgattt ttctgatatc tatgagttaa
     1321 aggtaaaata gtatgagtgg cgttattcct gtcagcatta gcaataaatc gctcgatttt
     1381 tataaaaatc aagtattcca gtcttttctt gatattgaag ggctaacaga aattgctgtc
     1441 aaccgtcctg gtgaaatctg gacggaaatt aatggtgact ggacattcca taataatgac
     1501 agtgtgacct ttgatttttg taaacgtttt tctcatacgc tggcatcatt tcggggagat
     1561 gaaatcggcg agaccaagcc attactttca gcaacactgg aatcaggaga acgtgtacag
     1621 gtcgtttttc ctcctgcatg tgagagaaat actatttcaa tcactatacg taaaccatcg
     1681 acacggcaga taacacataa agaatatgtg gcgaacggtt tttatgatta tgtccagtca
     1741 ggaaaaaaac acaggacata cgatgatgag cttcttgatt tattcagagc gagaaatatc
     1801 gctgctttta tggagcttgc cgtagcagca gggaaaacca ttgtatttgc cggagcaacg
     1861 ggttccggta aaacaactta tatgaagagt cttatcgatt tcataccatt gaatacacgg
     1921 ctgattacga ttgaagatgc agaggaaatc aaatttttta tccataaaaa ttatgtgcat
     1981 cttttttacc cttcggaatc cggcagtgat tccggctcta tcataacagc agcgaaatta
     2041 atcaaatcct gtttgaggat gaaacctgac agaattttac tggcggaaat taaaggtggc
     2101 gatgcctggg attttgtgaa agttgctggt tctggtcaca gtggaagcat gacgtccatt
     2161 cacgcggcct cggcaaaaga tgccatcatt cagatggtga caaaatgtta tcagaacaga
     2221 gaatgtcaga atcttccttt tgatgttctt cgtaaaatca ttatggacag tattgatatt
     2281 gttgttcatg taggcaggga tggtgttgtc aggcatatga gcgacatcta ttacaaaggg
     2341 gctgaatgtg aaaactttta ataagtcgca atctgttttt atattcgctg ttatgcttgt
     2401 tgcggcatgg tttgcaggtt catttatttt ctttgtgctt tacttcagcc agataaagaa
     2461 tctgaaacca ttgaaggctg tgttcagatc cattgatgcg tacaggatgg atatttttac
     2521 agacagcctt actttatctg ttgtgactac ggatatcaga gttttagcct ttgttgcact
     2581 tggagcagga tttgtcgtgt ctcttatcat ccctgttgcg gcgttgataa aactgaacga
     2641 gaagaaagaa aatattttcg gtgatgcgcg atttgcaaca attaatgata ttcgtgagtc
     2701 taacagtttc acgcttgacg gtgacgaaaa agacgggatt atcgttggta taaaagacaa
     2761 gaaaatcata cgctatgtgg gagcagcatt cagtgctatg ggagccggaa caagggctgg
     2821 taaaggtgct ggtatagtca tcactaatct tatgaagtac tggtggagtg ttattattct
     2881 cgatccaaaa agggagtgtt ttaatatcac cagtcttatc aggaaggtga ttttaggtca
     2941 tgaggtttat aaatttgatc cctttagttc ggtaacgcac cgctttaacc ctttgtatta
     3001 tgtcagcatg ggaacgagtg aaggttttaa tcagcttgaa aatctttcgt tgattattta
     3061 cccctataag acagatggtg cggatgctgg tagctatctt aataagacgg cgggtggtgt
     3121 ttttaaatct tatgctgtcg ctttatggtt tatgattaag aatgataagg ctggactaaa
     3181 aacgcttgat attgagcctg tcttctcaat gtctaaaatt aatcagcttt ttgagcgtgc
     3241 agaacccgaa catcttcttt cattcatgaa tgatattcgt tcagaattaa gggggaaaga
     3301 caaaacgctt gctgatattg gtgtcgctgg ccttaaggca ttcattgaga tggaggataa
     3361 aacaaaagca caactcaaaa gcaactttct taatggatta tcaccgtttg ctaaccccaa
     3421 tgtggcaaat gctactgatg gaaatgattt tgatttgcgg caggtgcgga aaaagcggat
     3481 gacaatttat ttctgtattt ctggtgataa tgccaggttg gctgaaaaaa taacaaatat
     3541 tttctttcag cttgctatac aggtgaatct tgaaaagatg ccaacggatg atccggaaat
     3601 aaagcatgac tgcctttttc tactggatga attcccgtca attggtgctg tagattacat
     3661 caagagtaaa tctggtctca ttgctggtta caagctgaaa ttgttgattg tttaccaggt
     3721 cggctctcag cttgaggaaa tctacagtta tgcaggcagt aaaacattac tggcaagtgc
     3781 accctgcaaa attgtttatt ccgcttctga tgttaaagat gcgcgggagt tatcagaagc
     3841 gatggggaca cgaacagtaa ctatcggttc gaaaagcaaa agtcgtagtc gtggaggaac
     3901 atcgagatct gaatcagaaa gccttattga gcgtccactg gtaacaacaa atgaactact
     3961 tacactgaaa tttagtgaag aaatcctggc aatgaaggga gaaaacccga ttcgttgtga
     4021 aaaggcattc tactatctca atgagtattt ctttggtgac tttgtgaagg ttgctccgga
     4081 acttgcaaac attttcccca ggaagaaagg aaagctggtt atgcctccac agaaagtatt
     4141 tgaaaatgaa atcgtcgcaa aagggtatct ggtagtaaaa gatgttcctg atctcgataa
     4201 agcggcataa aggagaacat gatgaagaag ttagtgtttt tattgatttc cattcttgct
     4261 ggttgttcat caccgccaga gccaacaccc gtgcagtttg aaaaggcaaa tgaagtgatc
     4321 aatccctcat tgccatatgt ccccgatttt cacggtgtca taaagtcaga cgttagcggt
     4381 aaaggatggg tttatgagat cacttcctta tccggagtac aggccagaac gccgacgttt
     4441 tattacgctc tggctcatgc ggacaggatt gtagtgacaa cacatgatgc tgggctgtgg
     4501 tttcgaatca gagatctgtt gaagatggaa ggggcaacag cagtcgtgga atggcgaaac
     4561 gagaaaagtt ttttacctga acaggtaaaa atagttttca ttaaagcaca aaatgaggtg
     4621 aaaaatgact ggaaaaaata gagtggtttt accagccttg ctggcgttag gattaatatc
     4681 aacaaatgct aatgcatctg acccttgcgc ttcggtactt tgcttatatg gaaaagcagt
     4741 cgggcaaggt ggagggagtg aatgcagaag tgctgaaaag gattttttta atattctgaa
     4801 aaagaaaaaa ggaagcattc gctggagtaa aacttttgat gcccgtaaag catttctgaa
     4861 tcagtgttca acggctgatc ctgctgctat atcaaaaatc atgagtaagt ttggtcgtgt
     4921 cagaggttaa ttaaaacctg ataaccgcgc caacgggtat cagggtactg caagtatcac
     4981 atttcgcgcc aacgaaatgt gatgattagt tacaatcact gaggttagaa atgaaagtaa
     5041 ccaaatctat tatagctatt ttattgtgct tatctgctgg tagtgcataa gtggatgtaa
     5101 taaaaaatgc tcgtgccttt ttgaaaggaa ccctcgatct gagcgttcag gaagcagatg
     5161 aagatataga ggaaagggaa gaactttata aaaagaacgg ggcacaacct gactatttaa
     5221 gttacctgga ggattaacac atgcaatatg cattgtttga tggatttgaa cgaaagtttt
     5281 tgctggatgc tcttgaattc ggtgttctga aggactggaa agaaaatccg gtaaaagaac
     5341 ttcctgatat tgatgaatct gctcacccct tccatatctg ttatggtgga tatttattaa
     5401 accctggtgt ttcagattca gatattagca gaaaaataaa agaccaggca ggattctggc
     5461 tggcagctat tgatgatacc cgcatggatt gtcattcaat agcttattat gatattcaca
     5521 cactccctct gatttcgtgt ggtcatcaaa agatagttcc ttttgccgcg ttaataaaag
     5581 ctgatgaatg catcatttca aaaattgctt cgtattctgg ttttgctgta acagcctttt
     5641 tgagaattaa agagtgggat attgcaacta atatacttaa ccgtgaggga atttttgcct
     5701 ttaatggctg tgagcgcaga ttcagagtag taagtaaaga taactggcaa catacagtat
     5761 cggaagaacg cgctatccgt tgtgccaaaa gattaattca atgtaaagga taacaaaatg
     5821 agacttttta tcgcagaaaa acccgcagta gcaaatgata ttgttaaggc acttggtggc
     5881 aattttaccc gccatgatgg ctggtttgaa agtgataacg ccattgtgac taactgtttt
     5941 ggtcatatta tcgaatcaca accgccggaa aactataatc ctgaatacaa agcctggaag
     6001 gttgaaacgc ttcctttacg tctttatccc gtgaagtatc agcctgttga aagtgcagca
     6061 aaacaggtta aaacgattct cgaacttatc agacgtggag acgtgactga aattgttcac
     6121 gctggcgatc ctgatgatga gggacagcta ctggttgatg aagtcctgga atatgcaggc
     6181 aacacaaaac ccgtaaagcg cgttctgatt aacgacaaca cgcttccggc agtgaaaaag
     6241 gcactggcaa atcttaaaga caatcgtgat ttcaaaggac tttaccttaa ggcgttggcg
     6301 cggtcagttg ccgatgccgt ctatggcttc tctatgacac gtgcgtacac cattccggca
     6361 aaagccagag gatatcaggg cgttctgtct gtcgggcgcg tccagactcc cgttcttggc
     6421 ctgattgtga atcgtacccg tgctaaccag aaccataaat ccagttttta ctacaccatg
     6481 accggagtct ttcagcgtgg tgctgatgtt atcagggcga actggaagcc aggggaattt
     6541 gctccgctga cagaccgtaa attacttgat aaagcgtggg cagacggaac ggctgcatcc
     6601 cttgcaggaa aaccggctac agttgaagca gcagcaactg atgataaaaa aacggctgcg
     6661 ccgttgccgt ttaatctggt cagactccag caatacatga acaagaagtt taaaatgacg
     6721 gcacaaaaaa cgctggatat tacgcaacaa ctacgcgaaa aatataaagc aattacgtat
     6781 aaccgctctg actgctcata tctttcagat gaacaattca gcgaagcgcc gcaggttatc
     6841 gatgccctga aatcagtctt tcctcagtcg ctggatattg attctgcacg taaaagcaaa
     6901 gcgtttaaca gtgcaaaggt gactgcgcat actgcgataa tcccgaccgc cagtgtgcct
     6961 gatgttaacg cactcagcac cgacgagcgc aatgtttacc tggcgatcgc acaacactat
     7021 cttgttcagt tcatgcctga aaaagcatac caggaagtat cggttgccat tcagtgtggt
     7081 gatgagtcgt tctatgcccg tgccagaaaa acaactgaca gcggatttga ggcgtttctt
     7141 ggcgcggaaa ccacagacga aggtgaatca gaagataatg atgattccgc ttttgaactg
     7201 ctctgtaaaa ttcgcacagg agaaacactg acgacaaaag aagttattgt taatgagaag
     7261 aaaacaacac cgccgccgtt attcaccgaa gcctccttgc ttgctgcgct tgttcgtgtc
     7321 gcggattttg tcactgatcc aacaattaaa aaattgttga aggataagga taaagacaaa
     7381 aaagatgaac atggtggcat tggtacgcca gctacccgcg cagccattct ggaaacgctg
     7441 aagaaaagaa actatatcac gctggaaaaa gggaaactta ttcctactga taccggatat
     7501 gcgcttattg atgccctgcc tggtatagcg gttaatcctg atatgacagc attatggtct
     7561 gaaaagcagg ctgccataga aaatggcgac ctgacggttg aacagtttat taatgatctg
     7621 tacggtgaac tgacaggcat gatttctgat gttgacctgg gcgagatgaa gattgaaccc
     7681 gctgcgccag cagggcagtt tcaacgcctg gactctccct gcccttcctg tggtaaacat
     7741 attgttatca ggccgaaagg ttatttctgt accggatgtg aatttaaaat ctggagtgag
     7801 ttttctggta agaaaatcac ccaggcacag gccgaaaaac tggttaaatc agggaaaacc
     7861 gatttgatta agggatttaa aaagaaaagt ggtggaacgt atgatacagt tcttgtcctt
     7921 gaggataaga aaacagggaa gctgggtttt ccggcaaggg ctaagaagtg aaaacaaagc
     7981 aggaatggct ttttcagtta agaaaatgta catcaagaga tactcttgaa aaagttattg
     8041 agattaaccg ttacaaactg cctttatcag aatcagaggc attttattct gccgcagatc
     8101 atcgccgagc agaactggtg atgaataaac tttatgataa ggtgccttcc ggcgtctgga
     8161 agtacgtaca ctaaataaga ggattaatta tatgagcgaa ctgactaaag aagatgaata
     8221 cggcattatc agccgtacta tgatgaatat tcgttcattg cgtgtttttg cccgtgagat
     8281 tgattttgag cagttgctcg aaatgcagga aaagctcaac gttgttattg aagaacgtcg
     8341 tgaagatgct gaacgtgaag cggctgaacg ccaggaactt gaagccaaac gtcagcaggc
     8401 cattgaatac atcatatctt taggtcttga tcccgaatca ctccttgctc ctgtaacagc
     8461 agatacagta aaaaccaggc ggaaagccaa aggcggagta agaaaggcta aataccgctt
     8521 taaggatgaa aatggcgaga tcagagaatg gtctggtaat ggcaagcgtc cgcttgcttt
     8581 acagaagctg cttgatgagg ggcatttcat ggaagatttc ctcattgaga aaactaagcc
     8641 ggaacaggca gaataacccg ccaagtatac atcacacagg gcactgttgc aaatagtcgg
     8701 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
     8761 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
     8821 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgttga tcactccacg
     8881 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
     8941 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
     9001 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
     9061 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaacgtg
     9121 aagaagtggc agatcccgcg attcatcaac acggataaag cgcccgccta tggtcgcgcg
     9181 cttgctctgc tcaaacgcga aggccggtgc ccgtctgacg ttgaacaccg acagattaag
     9241 taccggaaca acgtgattga atgcgatcat ggcaaactga aacggataat cggcgccacg
     9301 ctgggattta aatccatgaa gacggcttac gccaccatca aaggtattga ggtgatgcgt
     9361 gcactacgca aaggccaggc ctcagcattt tattatggtg atcccctggg cgaaatgcgc
     9421 ctggtaagca gagtttttga aatgtaaggc ctttgaataa gacaaaaggc tgcctcatcg
     9481 ctaactttgc aacagtgcct cacacagaca tacacatagg tatgtctgtg tgacatacct
     9541 atgtacagtg ccagatggcg ttgctacgtg aaaaataacc taccctaccc ctgctttttt
     9601 cgcagatttt acgtagcaca ccgttgctat agcattcatc taaaaagctg aatgtccttt
     9661 tctgtcgctg ttgcatttta attcactgaa aaatattgag taatgaaaat gccggtaacg
     9721 cgtcaagctg ttggccaacg cgacaggcag tattggaagc aacaaaaaaa atgaaagaaa
     9781 aatccctcaa atgaaggaga aaaaacggaa ctacagccga acgacaggca caaaaaaacc
     9841 cgactggtaa tcgggctttt ttgcgttttc cagtcttgag ttggtggctc tgaccggaga
     9901 aggttactct acacctaacg tttacatttt attcactatg gtgctgtaaa gcaactaaat
     9961 gtggttaagg aaccacattt agtggtgtaa cgggtgtttt gtgtagcgta taatcagtca
    10021 gttagtcatg tagttggctt gcaatcctgc tgttcccgta tctgcgggta tgaacgattt
    10081 taaagtcctg gctggataaa caggtggtgc tgtattcggt gctctggcag gaggaggtca
    10141 ctctacacct aacaatgcga gagagccgtt gtgacaggca gtgtatgagc aagcaacggt
    10201 gatttcctcc agttttactg gagaaacgga tgattaatat gttaatcatc gttctcagag
    10261 ctgtggtcgc cgttgcaaac gcgctgattg cagttctgga actgatccgc caattcatcg
    10321 attgatgaca acggaaacgt aactaagggc aggaagtgaa ccgcttcctg ccctttaaca
    10381 aaaaaggagg atttatggat tttatgagca ttttggggcg ctgtttactt cttcctttcc
    10441 ctgttgcgat gtttgtcggt tgcctgtttc ctggcatcga tgatcgctta tccggaatgc
    10501 ttatgagtgt tgctgtggga tgtttatcgt ggtatgtcac cagaccgaag aaccgacaga
    10561 caaaggccgc gtaagtggcc tttaattggg atacccttta gttacaacat tctaatagct
    10621 cattccatcc tccgctcatc cagcaaacat aacctccctg cccctcccca ctacagaaag
    10681 tggcgcgccc cgcccgtacg ggcggaatct aatcgcttaa ttttgaatgt tgtaactaaa
    10741 catactcatt gctgtattgc cacaaggttt cgcagcaagg gaggataccg aaagtaacgc
    10801 tgtcactttt gctttcaagt gaccaaccaa cgggtacatc acactgacat acaataatgt
    10861 atttcttcat gatgtacatc attgtaattc attgtgacat acactatagt atgtccatgt
    10921 gacatacgca tgcgtacatc acatagaaat acacatgagt aaatcactgt gacatacaag
    10981 gggtactatg attatcgtaa tcggtggtga taaaggcggt acaggtaaat cacatctggc
    11041 aaccaactta actgtttgcc tctcacaaca gggaaagaga acggggcttg ttgaaacaga
    11101 cctgaatggc tcgaccaaaa aatggaataa acgcagagaa caggccggat taccgcccgt
    11161 cgcattaaat gaagcctatg gcgacatcag tgccaaaata acaaaaatgg cagatgtagc
    11221 agaaatcctc attatagata ctgccggata tgacagcacg gagttcagaa ctgcactaaa
    11281 agttgcagac attgtgattg tcccgatcga cccgcttgcc caggtggaag cagacagtct
    11341 tcagactgtg acaaaaattg tcagggatgc gcaaaaaata aatcccaggc tggctgcaca
    11401 tgtgctcctg tataaatgcc agccaaatac gttcagtgaa caacaagaat taagagactc
    11461 tctcaacagc catgattact ggcttaaacc aatgaaaagc acggtttctt ttctccgggc
    11521 ctttgtaaga gcaatgaatc agggaatggg ggttcacgag ttaaaaagta atgttagcgg
    11581 tgccagccag gcaaaagcac aaatcgaact cttgctgaaa gaactggaac tgtaaatcat
    11641 agtgacatac actaatgtac atcatcatga tatacattag tgtgaaacaa tcagattaga
    11701 aggtacatca tggcaaagct ggaactacca gaactagaac aaaaccagga agtgaacgcc
    11761 acaaaatttg ttcaggacgc aggaaaacgc cccacagaga acaaaacaaa actggcttca
    11821 ttccgcatac cgaatgaatt gcttgaattc attgataacg aatcaaaaag actgcatcgt
    11881 cccaagacaa aaataatcaa agcagcatta ctggcttatc aggatctgga cgaaaacgaa
    11941 ctcattaatt tgtggttcag agctgacaga aatgattaat catagcaagt ggcccaataa
    12001 actctttatc attttctttt agatacttat ctgaatgtac taacttcatc acttttaaat
    12061 gtagttgtga ttcattttta gttactcttg caataaaata cccttgtaca gttgttgcac
    12121 gggtatttag gagcactaga tgagccttat cgatttaagt ttatcaggat tatcagaacc
    12181 aggaacaaaa cttattgaaa aaataagtga tgctattggt gtgctttatg aacctacaag
    12241 aatcaggaaa aaagcgaagg ctgaagctga agctaaacgc acagagctaa tttctaggtt
    12301 agagcttgag ggaatagaaa aacgggcagt tgagcgtttt ctcaaacgtg aaacgaaacg
    12361 acaggaaaac attgaaaata taacaatgca agcagcacaa agtctatctg agagtgataa
    12421 tgtttctgat attgacgaag attggataga ggctttcttc agggagtgtg aggatattag
    12481 tgacgaacaa atgcaaatgc tttggggaag aattttatcc gaagaagcaa agtcaaaagg
    12541 ttcgtttagt cgtagaacct taaaactatt atccactata agcaaagaag aagcgaacct
    12601 tattacttat tttggaaagt ttgtttggca ggcaaacaaa ctcactccaa ttttatttac
    12661 cgatgagaat ggagatactg aaggtataac tttcgataaa ctttcggttt tagactcctt
    12721 aggcgttatt caacaaggca ttggttatag tttaagatac aaattataaa tactctcaag
    12781 tgtatattca gtatgggatt gcgcaatgat tgcctaataa aatttctgaa atatttctgt
    12841 atcgcataat tttttatatc agataaattg tactggattt cttaaaaaat tgcagtatga
    12901 ttgccgcaat tatcccaccg tttatttttt gagtagtttc tcatgatgca gcatacttct
    12961 gtgtggtacc gacgctcggt cagtccgttt gttcttgtgg cgagtgttgc cgttttcttg
    13021 accgcgaccg ccaatcttac cttttttgat aaaatcagcc aaacctatcc catcgcggac
    13081 aatctcggct ttgtgctgac gatcgctgtc gtgctctttg gcgcgatgct actgatcacc
    13141 acgctgttat catcgtatcg ctatgtgcta aagcctgtgt tgattttgct attaatcatg
    13201 ggcgcggtga ccagttattt tactgacact tatggcacgg tctatgatac gaccatgctc
    13261 caaaatgccc tacagaccga ccaagccgag accaaggatc tattaaacgc agcgtttatc
    13321 atgcgtatca ttggtttggg tgtgctacca agtttgcttg tggcttttgt taaggtggat
    13381 tatccgactt ggggcaaggg tttgatgcgc cgattgggct tgatcgtggc aagtcttgcg
    13441 ctgattttac tgcctgtggt ggcgttcagc agtcattatg ccagtttctt tcgcgtgcat
    13501 aagccgctgc gtagctatgt caatccgatc atgccaatct actcggtggg taagcttgcc
    13561 agtattgagt ataaaaaagc cagtgcgcca aaagatacca tttatcacgc caaagacgcg
    13621 gtacaagcaa ccaagcctga tatgcgtaag ccacgcctag tggtgttcgt cgtcggtgag
    13681 acggcacgcg ccgatcatgt cagcttcaat ggctatgagc gcgatacttt cccacagctt
    13741 gccaagatcg atggcgtgac caattttagc aatgtcacat cgtgcggcac atcgacggcg
    13801 tattctgtgc cgtgtatgtt cagctatctg ggcgcggatg agtatgatgt cgataccgcc
    13861 aaataccaag aaaatgtgct ggatacgctg gatcgcttgg gcgtaagtat cttgtggcgt
    13921 gataataatt cggactcaaa aggcgtgatg gataagctgc caaaagcgca atttgccgat
    13981 tataaatccg cgaccaacaa cgccatctgc aacaccaatc cttataacga atgccgcgat
    14041 gtcggtatgc tcgttggctt agatgacttt gtcgctgcca ataacggcaa agatatgctg
    14101 atcatgctgc accaaatggg caatcacggg cctgcgtatt ttaagcgata tgatgaaaag
    14161 tttgccaaat tcacgccagt gtgtgaaggt aatgagcttg ccaagtgcga acatcagtcc
    14221 ttgatcaatg cttatgacaa tgccttgctt gccaccgatg atttcatcgc tcaaagtatc
    14281 cagtggctgc agacgcacag caatgcctat gatgtctcaa tgctgtatgt cagcgatcat
    14341 ggcgaaagtc tgggtgagaa cggtgtctat ctacatggta tgccaaatgc ctttgcacca
    14401 aaagaacagc gcagtgtgcc tgcatttttc tggacggata agcaaactgg catcacgcca
    14461 atggcaaccg ataccgtcct gacccatgac gcgatcacgc cgacattatt aaagctgttt
    14521 gatgtcaccg cggacaaagt caaagaccgc accgcattca tccgctgatt tctccctgta
    14581 ttttttccaa acccaccgca cactccattc gtattatggg cggtggggtg gggtttgtta
    14641 tgccgtattt atcaaataaa cgcctacttg ctgagatgag tatcgctctt gtcatggcga
    14701 tcgttgccac gctgaccctt gagcacagtc agattgatct gatggtcgct gattggtttt
    14761 atctgggtat ggggcattgg atggttgcca agcaagcttt tttgccagat ttgctactgt
    14821 attctggact aaaaaagctg ctgatggcga tgctgatcta cttgctggtt gcgaccattt
    14881 gccgtgctta tcatgagaaa aagggcaatg ctatcactgc caagtggctt gtcccagtga
    14941 caaaatttcg cgtgcgtgag cttgcgtatc tggtgctgac tttgatccta gtgccgacag
    15001 ttgtcgcgtc attgaaggca tatactcatg tggtctgccc tgtgcatttg acgatttttg
    15061 atggtacgct gccgtatttg ccgatgcttg atagtatgcg taacaccatt cctgataagt
    15121 gctttcctgc ggcgcatgcc agtagcggat ttgcgctgtt tgcctttgcg tttgcgccaa
    15181 gtttgcgccg ccgtcgtggt gcgatcatca tcgtggtgat ggcattgggc tgggcgatgg
    15241 gctgctataa gatgattatt ggcgatcatt ttttgagcca tacggtggtg tcgatgatgc
    15301 ttgcgtgggc gatgtcggca gggcttgcgt gggtgttttt taagaagggt gaacaagttt
    15361 aatcggcttt aaaagtaaaa acggtcgtat cggttattac ggaacggttt tttcagttga
    15421 gtttcaaagc gataattcat caacctggaa actaccaata ggaacggctt tacttacgcc
    15481 tatcggtcaa gaattgatga aaatatgcgg ttccactcca gatattgcgt atttaaataa
    15541 attcctcaac aaaataaatg ttgaaggaag ccaggtaaaa ctatcaatta ttaatatgta
    15601 aagaggtcga gttacctgtc tgagttccac taaccataat taaaagcgac cgcaaaaact
    15661 gcccagtgaa aactgtgttg ccagtaatca caaatgcagc aagtactaat aaagagcctg
    15721 ttaagcccgc aaatatgtgg gcttattttt aattactctt catcatcccg caggaactga
    15781 caaaaatctt cgctccccat catatcgaca gtgtttttaa attcgaaaaa agaaattcct
    15841 gtcatagcac acacgcgctg gataatcagt tttttctttt gctgtctgac ggttgcggtt
    15901 tcaccaaact ggataacagc caggactgtt tcatcatatg atttcaggac aaaatcacag
    15961 gttatattct gcacttcacg gattaaatct tttacagctt cctcgctacc atgaggacga
    16021 acaaattcaa tgagccgtat tttgggaaaa acggaataat caggtataat attacgtgaa
    16081 agaatgccta aaaactctga ttcattattt gagagtaagg gtactttagt aaaacaatca
    16141 acctccccaa tggctgtaaa agcattggtt gtcaaagctg gattgttctt aataaaatca
    16201 gagaatacat tttcagctga tggcaaaaca acagcagaat tatgttgttc atctcgtttt
    16261 ttattacggt taaatatcat ttcatattcc ttttaaaaga gcctgcggca ggcacaggca
    16321 aaaacatcgt ttttggacaa tctgattcaa atgttaacat ttgacatcgg attgtctatc
    16381 ctgcattgtt ctttactgtc tgtaagatcg gcgctcattc ttccggcgtt ctcccgatac
    16441 caaaaagacg gaacgcaaaa cggctttcga tccaccattc aaaaatctgc tctgcgcaac
    16501 cacaaacagt taaggcgatc catgcagcaa gaacaacgta actactgaca tctgcaccca
    16561 tgtaccaggc gatagttaaa cccatcactg cgataccgcc taaaaactgc aacaagaaac
    16621 gaaatcccgc aagaatcccc aatggaataa caacaaccag ataaagggaa gcagccagaa
    16681 ggtatctgac aggtgtcagc agataaaaga acctggatat tttctttttg tcagaataaa
    16741 cggcatcatt tgcaacatcg gttgaatgat cattcatgct gtccctgatt ctttgaaaat
    16801 ctacgatatt atccttcata ttgttatgac ctcaattttc aggtgggtac attttatcaa
    16861 acatattagt aaactctgag tcttccctgg tatcgccctc ataaccaatt gtataagaga
    16921 actttaattt ataaactttt ctcccaattt tttctgaaac tacgaaactt aagttcttta
    16981 cttccgtatg tttcatgatt tcttttactg atttattaag aacatctctt ttaaaagcag
    17041 gataatctgg atacttataa tccttcaccc cccccgcccc tattgtataa agattcaatt
    17101 catctttaag ttcatctatt gtcatttcaa aactattcgc gcgagaatta ttactatata
    17161 ttttacgaat gagttgatat agtgatgcag cattaacact tgtcagtcga acaacagata
    17221 ataaaacttg cgttgtataa cgtttttctt caccaataag tttacataaa aatcttttcg
    17281 cttctttcgt aaacttgatc cccactctac cttcattcct gtaataagta gaaaaaacag
    17341 tcagactcaa attcattgaa tcaggtacat tacttttagt gaacttaaat cccaaatcac
    17401 tactcagttc taatatttca tcatgtttca gcttaagaag cgtcgtatct aatatattag
    17461 agccatccct caaagcaaga taagccgcat caggcttaac ctgaacccat ttaatgtaat
    17521 cggcaactgt cacataaaat atgtgatcat catcgaattc atttttagag tttatctggc
    17581 acatggctaa aaataagact tttttagcag ccataggcaa tgttgacagc gttgaattca
    17641 attcatttcg atgtctcact tttgttaagc gagaaaggga agtcttattt gtcgtcattc
    17701 tcattgttcc aacactcttg ataatcagac tttaacacca gaaacatgat attacaacac
    17761 aatcaatatc atgtttaacc gagtatcttc tcatgtttca tatctgtgat ttatctcaca
    17821 aaggtaatcc atgctcaaaa tccacactgg attcaaggca gattaacaac agattcaaaa
    17881 tagattcacc agaaagaaca acgaattagt attcttatga cacattttgt ggatatcacc
    17941 gaggaagctc tcatgtttaa ccgaggaagc tctcatgttt aaccgaggaa gctctcatgt
    18001 ttaaccgagg aagctctcat gtttaaccga ggaagttatc atgtttaacc gaggaagctc
    18061 tcatgtttag acaaaaaaca cgcttaaaat caataaatta gagctactct aagtttttaa
    18121 gtttttaaga aaaacaagaa aagataaggc ttttaatgcg tgcgaatatt caggaactgt
    18181 ggataactca acaaaaccag caacgatgat ggttccttac cataaatctg tcccctgatg
    18241 attattaccc gcaagctaaa acaaacatca tcaatcatta aaactcataa ctttcaataa
    18301 cataaaatca tcaaaaaaat atttgatgcc ttatgtatct caattttcaa gaccataaat
    18361 ttgtccctag ttaactttaa cttattgaaa taaatataat attcgtgcgt actgatccta
    18421 atgtcacatc gcaaaaattc cgcttcctcg ctcactcagt cgctacgctc ctgccgttcg
    18481 gctgcggcga gcggtatctc ctttctcaaa accagagaga ttcagatcat atgatccgtt
    18541 atttgatcct ttttggatct tccactaaga agccctgaat cgcgctctgc cgcttcgttc
    18601 gatacaacca tgaggaaaac catgcgaaat gaaaaaaatc gcttaaaatg cgattcagag
    18661 cgttttaggg aggttctgtt agttctcctg cattgcgccc gtagttcgct aacgctcaca
    18721 cgcgcagtac agcgccccgc catgctgcaa tgcaccaggg cggggtaaac tcgcttgcgc
    18781 tcgcgctaac tgcggcattc tgcggcgcaa atcagcataa tcatggcctg ctggtcagtt
    18841 gggctttgca gggtgaaggg atggcctgtg caccctggta cgttcgtcag cggctgcgcc
    18901 gcgcttcggg gcgatgtggc tcctgtgctg ccctggtatt ttcgttcgcc ccttccatcc
    18961 gcttacgcgg attaaaaaac gtgataacaa tcacttatcg agtaaaaaaa cgggagaaaa
    19021 cttgcaaata cgacttaaag gtcgtattat tgtttcatcg aaaggggaat ggcccctctc
    19081 acaatagccc agcagggcag gagattaaaa aaatgaatat ccaggaagca ttaaacgttt
    19141 ttggattatc cggcgaatta actgaaaaag atatcaaagc agcatacaga aaagccgctt
    19201 taaaatatca tccagatcgt aacccgttag gggctgaact gatgaaagcg gtaaatgcag
    19261 cttttgatgt attgatggca aatattgata aaataaatca gttccagagc actgatgaac
    19321 atgcacgata taattacggt gatgacctgg aaaaagtatt aaacgttctt tctggtttat
    19381 ctggtctggt atttgaagtg ataggtaact gggtctggat tagtggagaa accattacac
    19441 ataaggaaac tttaaaagaa atcgggtgta aatgggcggc aaagaaaaaa caatggtttt
    19501 atcgtccaga cgaacataaa agttactgga atcgtgaaga acacacgata gaagaaatcc
    19561 gcgcaaaata cggtacaacc ggacagcgca gggcgacagg gtggcaacgc gtggaaacca
    19621 gagcgtaacc agaacggggg cgagaagccc ccgatattat agccagcccg tcttttgggg
    19681 catcgaaccc aaaaagacgg gaacagcagc cacgtagcaa aaagggaaac agatgaactc
    19741 tttttttgaa caatatcacc ctgtctttga agttgtctgc cgtatcctgg ggaacggctg
    19801 gcgcgtgaac aaacttgatg attgttcgtc tcgtataaaa ctgacgtcac cgcagtttaa
    19861 aaattactct gtgcatattc ggatggaaaa agaccgcttt tctgtggtgg ggagtgtgga
    19921 tagtcgttcc tggcgtagtc cgcatcatgt ttgcacgtta tccagaaaac ggaatcccgt
    19981 tgatattgca gccgatatcg agcgaaaaat tctggtaaat gcttcgcagg aggtgttaca
    20041 ggcgattgag tatgagaaac accaggtgga aaaaaaggac gaaattctga tcctgaaagg
    20101 tatgttatcg caacttgtcc agcttgaaag ctggtatggg gcattgacag gctttaaagc
    20161 tgaaaacgga ttgaacggta aagtcaccga acagggcgac agttatgatt tacagattcg
    20221 gggcttgagt atagatcaac ttgttaaaat tacagggtat ttgaaacagt tatgagaaaa
    20281 aataaaggtg atgtgactta ttttcttgaa aaagaaggtg acaattacag gcttacaaaa
    20341 aggataaaag ccagaacaaa tgttaaaatc gggaacaaaa ctacgaaaat tacattgtat
    20401 gatgctgtac tgaatgagaa tgaattacaa catattgatt ttacgtgtgc cggattaagg
    20461 gaagacgatg agacgccagt taagaattta attaaggagt ttatgttaaa tgaaacccga
    20521 taatacacct gaaaatgtaa aaaaattacg tttaaaagca ggattaacac aaaaggaatg
    20581 cagtcatatt tatggtgttg ggctaagaac ctggcagaaa aaagaggaag ttaatactca
    20641 aaacagtcaa agtctgtcac tggttgagtt tgagtttctt ttgttacttg caggagaaca
    20701 tcctgaatac gtgctctgca aaagggagtc aaaataaaat gacctgtgat ggttttatat
    20761 tagtacctgg agttgcgctc gtagctcgct gacgctcaca cgcgcagtac agcgccccgc
    20821 catgctgcaa tgcaccaggg cggggtaaac tcgcttgcgc tcgcgctaac tgcggcattc
    20881 tgcggcgcaa atcagcataa tcatggcctg ctggtcagtt gggctttgca gggtgaaggg
    20941 atggcctgtg caccctggta cgttcgtcag cggctgcgcc gcgcttcggg gcgatgtggc
    21001 tcctgtgctg ccctggtatt ttcgttcgcc ccttccatcc gcttacgcgg attaaaaaac
    21061 gtgataacaa tcacttatcg agtaaaaaaa cgggagaaaa cttgcaaata cgacttaaag
    21121 gtcgtattat tgtttcatcg aaaggggaat ggcccctctc acaatagccc agcagggcag
    21181 gagatttaaa atgaaatgct catctgtttt tacttcaacc actaatcatg ttttcacatt
    21241 cgaacgagta acgctttgca ctattatcct tatgcataag gatactggac aacagtatgt
    21301 tgtcatattt actgataaca ataaaattcg tgactataaa gccggaattg ttcctcagtt
    21361 tggcgaactt aagcagagtg atgttgattt agtccttttc tacagggacg aatatgaaaa
    21421 atattttgat tcattaaaag atggcgatga atgcttgagt tttaaaggtt ttattgagtg
    21481 tctttgttaa gcattatgaa tgcaggggca tccctgcatt ttctggaaat ttcaatattg
    21541 attctgaaat tgtctattaa catcaatgca agggaaattc atgaaaacgt taaaacaagc
    21601 cgcaatgcag tttgcatctg atctgcgtaa taatcgctgt tttaaagctg cttataatga
    21661 tgctctgtta gagcttgatc gggaagattt aaacgccatt gtacaatgca catttctttt
    21721 tgagccaaaa cgaacattag ccgaacttga taaattaatt aaaaagatag aagtagaaga
    21781 agattatgag ttttgtgtat cccttgataa aggtaaagga ttacacgaag tcagaacatt
    21841 taaaagtgag cgtgaggcgt tggatgctta tcgcattttg actctttacg gatacacagt
    21901 aactatagaa aagaaggagg gtaatgactg actattcaag gtgtgatcag gttgttccac
    21961 caacctgatc acgttatcga caatcaaaac tgaatgaggt ttttaaatgt caacgcggca
    22021 gatcgtacag cttgctgatt cccttgtcaa aggctgggaa accagaattc cggcaatgaa
    22081 atttagcgaa tgggatcaat tccggtggtg gttaaactac ttgcaagggt atgaaatgtt
    22141 ttaaataaaa aaaggggctt tctgcccctt tgtatttttt gacaaaaatg ccatgtgtaa
    22201 ctataattag ttgcatatct ataggggatg gatttatggg gaaaacagat aagctactgg
    22261 caaagttttt aaacagtaaa aaaacgtttg aatgggatga actggtcgtt ttgttttcct
    22321 ctttgggata tgtcaaaaag gaaatgcagg ggtcaagagt gcggtttttc aatgctgaaa
    22381 tcaatcacac catattaatg catcgtccac atccggaaag ttatatcaaa ggtggtacgc
    22441 tgaaagcaat taaacagaat ctgaaagagg ctggattatt atgaaacatt taaaatataa
    22501 aggatattta ggcacggttg aacccgattt tgaaaataat gtcctgtatg gaaaactggc
    22561 gtttattcgg gacctggtaa cttatgaggc ttctacatta gctgaactgg aacaggagtt
    22621 taaaacatct gttgatctgt atttacagtc ttgtgtggag gatggaaagg aacctgatac
    22681 cccgtttaaa ggcgtgttta acgtcaggct tgatccagaa ctgcatcgtc gggtagcaga
    22741 aatggcgatg gaagaagatt tatcgttgaa tgcctttgtt aataaggctc tggaaaaaga
    22801 agtcagcaat catcgcgcag gggcttaatt gcccttgttt ttatgccctg taagggcatg
    22861 ggaggcgctt tgcgccggaa gccccgcagc gcagcgagga cttaaaaagc gttacggctt
    22921 cagccgtaac aattcccagc cctgccaggc ctgttttttc tttcacaaat ctttcagcta
    22981 tacgtttgcc tgatggtcga agacgaaaga atctttcagc aacgaaatct ggacatatgc
    23041 agcgacgatt ttatagccag gcacagtgcc acatatggta caatcaatgc aggatagaca
    23101 atccgatgtc aaatgttaac atttgaatca gattgtccaa aaacgatgtt tttgcctgtg
    23161 cctgccgcag gctctgttgg ggtgacttat gaagatgata gcgttcaggc taagtgatga
    23221 tgagttaaaa tttgcagaac ataacgctat tttaagtgga tttaccagta tcaatgcttt
    23281 tgctaagcat aacgtgctta atattgaaac caagcctgtt aacatcccag ttaataatga
    23341 accagcaaag ctggtatcag tgcgtcttta ccctcatgaa atagaacttg ttaaacgtaa
    23401 tgctgctctt cacggcatga gcatgagcag agaaattgct attcgtgtgc gtcagtcttt
    23461 gttaaagagt gaagtctgtc tttaccctga tgaagttaag gaacttaaaa aactatcaac
    23521 tgctgtagat cgtgttggtc gcaacattca ttttatcata aaaggtgaaa gattctgtac
    23581 ggttaacgat ccagatttca gaaaagacgt tattgaagtg attgaactgt gtaagcaaat
    23641 tgattcaaaa cttgaaacgt taaccaagag tgttgttaac agatttgggt gatttatggg
    23701 tgtgtatgta gaacaggaat accgtattaa acgcgctaaa gggactgggc gtgatccgaa
    23761 atctcctaag ttatcgggaa gacatataca tgccagtaaa tcggctttta atcataaagt
    23821 cagacatggt gcggacaaga aagcatatac ctggacacct acaaaagaag tcacttttaa
    23881 aatcactggc tcaggtaaaa cagccgcagg catcaaaaat ggtattgatt acattacccg
    23941 taatggtgaa cttgaggctt actgttatga tggaaaaggt tcagagcaaa cagggaaagg
    24001 agaagacttt aatagggagt ttacttcaac tttatctaaa ggaaatgatt actccaggac
    24061 atatcgtgga gaaaacatcg atcatgtgaa aaacatggtc ttttcaccac caccagaagc
    24121 tggagtaagc agggaagacg ctctgaaagc tacggtagaa tttcttaaag agacttatcc
    24181 aaaccatgct ttcgttgccg tataccatga tgataaagaa gatcatcctc atgtacatgt
    24241 taatattaaa cttcgtgatg aagaaacagg aagaagatta agacttacta aatctgaaac
    24301 caggaagttc agaaacggtt ttcatagaaa actgaaaggt atgggatatg atgttaccgc
    24361 tacctggaaa aaagatcccg aacgtaaacg ggagattgag cgattacagg ctgaaaatcc
    24421 gaaaagactc cggaatgttt acaaggtggt ggattttggg gagacttcat atcagaataa
    24481 ggcaggggaa aaacgaacac ctttcctcac ttatgaaact ttaaaaggtg gaaaacaggt
    24541 tacgatttgg ggtaaagacc tgaaaaatca tttcgagtct gaaaagctcc agccaggaac
    24601 gcttatcaaa gtaaaaaaac tggctcctac gcttgtacgt agtccaatgt ttaatgatga
    24661 tggaactgtt gcaggctaca gggaaacgca tcgcaataac tggcagattg aaaatattgc
    24721 ccttgagcgg aacagagaac gtcaggttca tgagcgtgaa accaggcagc cggaagagag
    24781 cgacgttaaa aagcagctat ccagaaaaca tgagcaaggt cacaatatcg ggtttgctct
    24841 tgaacacggg tttacaaagg attcagaaga acataaaaaa ctaagaatac agcaagagag
    24901 gaactggaaa ggcctgggat tttaatttga ttttggcgca ggtgaacaaa gcggctactt
    24961 tgctcacctg ctgatcacaa caacctacga gggaggttat catgactgag catgatgcta
    25021 tctgcatttc tggacttcat caaattttta gtgatgaaga acatttgagt gagcaacaaa
    25081 aagacattat tctgatgtac gcttatggct ataccctgaa tgaaattgct gatttcaaag
    25141 gactaaagcc ttcaactgtc agaaaatatc tcgattctgt aagagctgaa ctgggaggtg
    25201 tctcgttggc agggatcaga acactggtac tcattcggac taatgccctt ctcgtgtcaa
    25261 gtctttccag gatcagtgag agggggaatt tatgaaaaaa tatctgcttt ttgctttgcc
    25321 gttcttcgtt gttggttgta gtgaagaagt aaaaagtgtg gattggtggg ggcagcatct
    25381 tacggaagct aaacaaaagc aggcagagtg tgagaagtct ggctctgatt ctcagaactg
    25441 taagaatgtt aaacaagcat tgtttattca gtctcagaag gatgctccag tccctacatt
    25501 cgattaacta atgtttcaat catactagag cgcccatatg ggcgcttttt tttgcatgat
    25561 cacacgaatc ataatatgac cattttgtca atgttatttg atcgtattgt tgagcattgt
    25621 tattttgcga atcatgttcg catctaaaac aaatccaaaa cacacttata gtggattgat
    25681 taaatgaagg agaaatcaga taagtcatcg gaaaaagggg tttatgtcac gataaaactc
    25741 gatcccaggt tgaatgcatt tatcgaagag aaaacgaaaa catctttgct tagtaagagg
    25801 aagcaaatta tttacttatt aatgcagagt atgcgtattg atggatatat ggagcgttag
    25861 ctatggcgtc tttattccca gccttgttgc tgtgtgcggc gtcagttcat ccggatacga
    25921 taaatgatat tgcacgggtc gagagtggct tcaatcctta tgctgtggct gaaattattc
    25981 ctgaaaggga acgcggtagc tatggcaaaa gtgttatcag tcatatgccc aaaagtaagg
    26041 aggaagctct aacagttatc agagaaattg aaaacagaaa aagacgttat tctgtcggat
    26101 tgatgcagat taccagcagt aatttttctt tttattccac gtccgcagaa aaactccttg
    26161 attcgtgtga aaacttgtct gttttcgaaa agattatcgt ggattgttat aaacgaggga
    26221 ggagcctgga gaatgcgctt agttgttact atacaggaaa ttttagtaac ggtaagcgaa
    26281 aagaaaaaga atttaataat acaagttatg ttgagcgaat tggttataca gggaacgaaa
    26341 aaaaatatgt tgtaccaggt acaagaagca acgggggaga acagcgaaaa aatcgttcac
    26401 acaatgcgtc agttatctgg cccgaaacca ttctgaaatc tgcttttgtt gataactcac
    26461 atccaacaaa agttattaat taacaggact tataaaatgt catttaagaa atcagtatgt
    26521 aatcgtatca gttcatctgt cactgctgta atgcagcgta aaaataaggt agttatggca
    26581 gtcgccgggg caacagttgc atcaccggcc tttgcggatg gattttcaaa agcggaaaca
    26641 ctgctggaaa aggttaaaac agggcttagt ggcctttcac tggtcacggt gactattgcc
    26701 tgtctgtggg tcggctacaa ggttctgttt ggtggaagca cgattcgtga atgctcacct
    26761 atcatcatcg gggcaattgt tatcgccagt gcggctgaag ttgcatcaat gatggttaac
    26821 taataaagcg gggacgatag tatgtctaca ctttataaag cgatgacacg ccctgctatg
    26881 tatgtgggtg ttcctgtcgt ccctcttact gtggttgcag gggcgttatt tctggcgggg
    26941 gtttatatca gcaagctgat ctggctggct attcctgtcg cggtttttgt tctcagaatg
    27001 atcactaaac aggacgacca catttttaac ctttactttc ttaagctgaa aatgctcgga
    27061 aactcagtgt gtaatcgttt ttttggcgca cgagcgttcc tctccggaca atatgaagct
    27121 gttgaaattg atgagtttgt taatgccatg aaactgaatg agcgaatcac tactggtaaa
    27181 tatataccct attcaagcca tgttgataaa aatattgtca aaacaaagaa cggtgattat
    27241 gtagcgacat ggcaactgat ggggattaac tttgagtcaa tctctgcgga gatgttagaa
    27301 actattgact cacaggttgc cactcttgtt cgttcatttt ccggactgcc cgtttcattt
    27361 tataaccatt catgcagggc gagtttttat gatgccttta ccacaaaatc aggtaataaa
    27421 tatgctgata taatatcaga ttgttactat ggttcgatga agaaaaataa attcaagggg
    27481 aacactcttt actttacgct gatctacaga ccagatgggc gtgtagaaaa actggaaaaa
    27541 cgcaaaaaaa gtattaaaga aaagaaagat gatattaaca ttcatgtgaa gagaatgaat
    27601 gaaatgatta atacattctc tggcgctctt gataaattta cctgtaaact tcttggtatg
    27661 tatgaggaga acggaaaggt attttcttct caactgtcat tctataatta tcttctgaca
    27721 gggaagttgc aaaaaatcag agtcactgat tctccggtgt ataacgttct tggtggagtg
    27781 gatgtatttt ttaatcatga taccggacag atatgccgca ttgatggaaa taaattcttt
    27841 cgttctattg aaattaaaga tttctgtagt gagactgcat ccggtgtttt tgatgtgctc
    27901 cagtattctg atgctgacta tattattacg cattcatata catccatgag caaatcagaa
    27961 gcattgagta ccattaaacg tgcggaaaaa cagttaaaga gtacagaaga tgatgcggtt
    28021 actcaattgc aggaactgga aaaggcaaaa aatgatattg tgtccggtga tatatccttt
    28081 ggttactacc atttcacgct aatggtaatg gcggacagta tcagggagct tgacgaatct
    28141 gtaagtaaaa ttacggcaga ctttactgac ctgggcatta ttccggcatt gtcaaccatg
    28201 tcattgccag cggcatattt tgcccaactt ccggcggttt ttcatttaag gccgcgttta
    28261 tctccggtat cgaatgtaaa ttttgttgaa ctggcatcat tccataattt ctaccagggg
    28321 aaacgcgata aaaactgctg gacagaagcg gttgctattc tcaaaacacc aagcaaacag
    28381 gcgtattatc tgaatttaca taactcagta ctgtttaaag atgaaacagg tgaaaagaat
    28441 cttgctaata ctaaagttat aggaacggcg ggttcaggta agacaatgtt tctgtcatat
    28501 cttgcctgtt cattacagaa atacaataac cccgaaactt ttgctgattc tgcaaaaaat
    28561 aagaagttaa cgtgtgtctt tcttgataag gacagagggg ctgaattatg tatccgaatg
    28621 cttggtgggg aatattacac cgtaaaaagc ggtgagccga caggatggaa tccttttgct
    28681 ctggaggcaa caaaacgtaa ccgtattttt gtcaagcaac tgatggagat tctgtgtacc
    28741 agaaatggtg aaagattatc gacaagagaa agattgctta ttagcgaaag tgttgatgct
    28801 gtgatggatt ttcctcctgg cgaaatgcgg gaatatggca ttaccagaat gcttgagcat
    28861 cttatgcaga gagatgatcg ggatgagcag gagaacggga ttatccttcg tttgtcccaa
    28921 tgggctaatg gtcaggctca tggctgggtt tttgataatg ctaaagacac attcaatatt
    28981 cagcatgtca ataattttgg cattgatggc accgagtttc ttgatgatcc gatggtatgt
    29041 gcaccaatta ctttctatct gctgtatcgc attacgcagc ttctggacgg tcggcgtctt
    29101 gttatcttcc tggatgagtt ctggaaatgg ctacaggatg aagcattcag cgactttgtc
    29161 tataacaagc tgaaaacaat tcgtaagctg aacgggctgg ttattccggc aactcagtct
    29221 ccggatgaaa tactgaagaa taagatttca cgggctgtgg ttgaagtatg cagcaccagt
    29281 atttatctgg ctaaccctga tgccgattac aatgattatg tggaagggct taagctgaca
    29341 ccagaagaat ttaatattgt taaaaatctt gatccaatgt cgagacagtt cctgattaaa
    29401 aaaagcagtc ttaaaaaggg ggatggtaaa tcattttcag cacttgcgac actcgattta
    29461 tcgggacttg gtggatatct gaaaatattg tctgccagtg cagataatct ggaaattttt
    29521 gaaagtattt atcatgaagg aatggagcct gatgactggg ttcctgaata tctcgaacgg
    29581 gcaatctgac aggtgaattt atgaaacgtg taaaaacatt gatgctgata tcattattga
    29641 cggcttcatt ttatagtagc gcaggaattc cggttgctat tgatgccagt ccggaatggg
    29701 ctgttgaagc cgcaagatgg acagaacgac tcaaacaatg gtccgaaact gcgcaacatt
    29761 atcagagtca gattcaggca tataaagacc agttagccac ggcaacaggt ataaggaata
    29821 ttgcggcatt cacaaatgaa ctaagtaatc tacaatcaga actgacaaat atttataagc
    29881 aggggaacag ttatatttct gattttacca gtaatcccga aggtgcattg agcagccagg
    29941 ctaaaaatct gttcagcaag tatggagcct tcgatatgtg taatacgggc tatgaacgta
    30001 atgataatct ctgtaaagca cggattgtgt caacggctgc cagtattgaa cagggaaatg
    30061 agatcaataa gcagttatca agtgcaatgt ctcagattca gagtttaagt agccgaattg
    30121 aggcttcaaa ggatatcaaa gaatcccagg atttggctaa cgcattacag gcgcagtcgt
    30181 taaaaatgca ggcgataaaa atgcagtatg atgtctggaa taataaaaat aaagcagatc
    30241 atgagatgtt ggtcacacaa gaacaagagg cctttattaa acagcagaaa gaagctgaac
    30301 cattaacttt tgattaaaag gtggatatat ggctcagggg tttttcgtta aatataattc
    30361 taccgttatg gattcggtag ataaaatcag ctccagttat caaacacaat ttgctaatga
    30421 tattatgtcg ttagcaacgg tatcggttac gttatatgta ctatggaaag gatatcaaat
    30481 acttgccagt aaaactcaga cgcccttgca ggatttagta tgggatctat caaaatttgc
    30541 aatcataatc atgtttataa ctaatgcgga tgggtattta actgcggcta ctgatgcgtt
    30601 gcaaggcatg aaagatggtt tttctggagg ggtgagtgtc tggcaaactt tagataactt
    30661 atggaaaagt acgcaaaatc ttggtgcgga aatatactct ttagataaat caacatatgt
    30721 taaagatcaa ggtgtagttg gtcagttttt aatatggact ggctcgctta ttttaatggc
    30781 tgttagtgtg gttgtttttt taacagcaga tgtaacgatg aaactcctga ttattacagc
    30841 accgatattt atcttttgtc tcatgtttgg ttttattcgg agcatgttta ataattggtt
    30901 acaatcgtta ttctccagta ttctgacagt tctatttgcc agtttagtga ttcggatagc
    30961 aatggatttt caagggatga tcctttccca tgctattcgt gctgcacaaa cagggaatgt
    31021 aaatcttgta tctacaggtg ctatgggctt tatggctggg tttttggggg ctttattggt
    31081 tcttatagct aaaggttttg ctgtccagtt agcaggtgca ggtgttgaag gtgctgttca
    31141 gggcgcagcc atgatggggc tgggtgctgc tggtatggct actggtaagt ctctgatgct
    31201 tggcggtcgt gctggtttag gttttggctt agggatggcc ggaagaacgg gcatgaattc
    31261 tttatcaggg aaagcaggga atctgatggg gcgtggtgcg cgaacagccg ctgaatgggc
    31321 gggtgaaaag ggatatcagg cgctggctcc gacaggtgct ttggggatga aggcgagaag
    31381 acttgcatcc ctggaaaaag cacgagcgag gaacgccgca tgaaaataat gaaacgtttt
    31441 ttttatgtga tgtccctgaa ctggatactg gtgttcgttg ataaacatat cacctccgga
    31501 tggagcagga agagaaagct aataacccag cttgttattt taatcacggg aattacctta
    31561 tttaaggtct taggtggaat cagtcagagc tggttgtgac atgcttcatt attttgtcat
    31621 gatactttat tttgtcctaa tccttctttt aggagtgttt acctgcttcg caggaataag
    31681 acttggttta gggcttgcgg ggaaatggag aaagggtatg gatcttcgct ttttgcttct
    31741 tattggtggg attctttttc ttcatcttgg cctttatctc ttaaaggtgt tcgtgaacgc
    31801 ttaataaggt gaatttatgc gatgtgtgat tcttttgttt ttcctgtttc agatttccgg
    31861 atgtaccaca aggaataacc ttccttctga tgtttccggc gaactggaac ccattaacca
    31921 cagccaggta atcagttatg agtgaaaaaa tcgaaaagaa gattgagaca gctaaatcct
    31981 tcgaaagaca actctatgca gaaaatgaac ggtctaagaa aaatgcatgg cgtgttgcag
    32041 tggcagcatc attactgtcc ttatgtcttg gggctgcaat ttgtgttctt gtgccattaa
    32101 aagaaaaaga gcttgctatt gtggctgttg gcgaaaaaac aggccgtaca gaactgatta
    32161 ctcaggttaa acaggaaaag atcctgcaat ctgaagcact tgggcggtat tttgtaaaca
    32221 cgtatattac tttacgtgaa ggttataatt atccgtctct tcagtatgat tatgagactg
    32281 tacagctata tagctcgaat acagtaaagg acgattatct tcgtctgtat aacagcgata
    32341 tggctcctga taaaatttat cataataacg gttcttacgt tgcggttgag gtcatctcca
    32401 atattatttc tgacgcaacc gcgccagata aactggcctc tgtgcgcttt aaaaaaacga
    32461 cgagaaactt cacgaccgga caggtttcag tttcatactg gacggctcgt gtgacatatc
    32521 gttttgaacc agaaaagtcg gttaaaagtt ccagtcgtga gcttaatcct ctcggtttta
    32581 ctgtaaccag ttaccagaca gatcgggaag tcagggggga atgatgaaga aattatttat
    32641 tgctacatgt ttattgcttc ctgggcttgt gtattcagca gcgactccgt taccttctgg
    32701 atttgatgca aggatgcaga ctgtgagtta taacggtgcg aatacgaccg ttatccggtc
    32761 aaagacagga tttctcacat cagttgtttt tgatgaaggt gaggctgtta tcagtgcaaa
    32821 ggctggtttt cctgccggat gggaaatcac aaccgatgat aatgttgttt acatcaatcc
    32881 gcgtcccgtt gttcaggaac aggaaggcga tgaaggcgaa aaactgaaaa aggttttcca
    32941 gccaacagaa aaggaatggg atacgaatct ttttgtcaga acaacaaagc ggatttacag
    33001 tctggatcta atccttctgt cagaagaaaa acaagcgcaa cctgcttatg ttgtacaatt
    33061 ccgctatcca tcagaaatag cgaaaaagaa tgcagaagaa gtaaggctgg caaaagaaaa
    33121 acaggaaaaa ctcagacaga agaaactgat ttctgaaagt tttgaaaaag ccgatgcgcc
    33181 taaaaactgg aattatttca tgcgagtcaa tgaaaaatat gacagtcgtc gtatcgcccc
    33241 tgattttgct tatgataatg gcatatttac ctttttaggt tttaactcag ggaaagtatt
    33301 cccagctcc
//
