LOCUS       NZ_CP101841             2076 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Klebsiella pneumoniae strain GN4549 plasmid pGN4549-6, complete
            sequence.
ACCESSION   NZ_CP101841
VERSION     NZ_CP101841.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29935323
            Assembly: GCF_029201465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Melano,R.G. and Seah,C.
  TITLE     Detection and prevalence of carbapenemase-producing uropathogenic
            Enterobacteriaceae isolated from patients in a tertiary care
            hospital in Jamaica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Melano,R.G. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029201465.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 05:34:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,613
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 52 of 132
            Pseudo Genes (incomplete)         :: 81 of 132
            Pseudo Genes (internal stop)      :: 15 of 132
            Pseudo Genes (multiple problems)  :: 15 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101841.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GN4549"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pGN4549-6"
                     /geo_loc_name="Jamaica"
                     /lat_lon="18.1096 N 77.2975 W"
                     /collection_date="2021-06"
                     /collected_by="Camille-Ann Thoms-Rodriguez"
     gene            372..758
                     /gene="stbA"
                     /locus_tag="NPH93_RS28045"
                     /old_locus_tag="NPH93_28030"
     CDS             372..758
                     /gene="stbA"
                     /locus_tag="NPH93_RS28045"
                     /old_locus_tag="NPH93_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006796897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein StbA"
                     /protein_id="WP_001039464.1"
                     /translation="MNKPADLKPRNLSAAVRLRLNEIENWLDRGLTRHEIAEILDSEYS
                     FSVTAKGLEMALYRTRQNRKNVLHNTHDKSSAKGAAESVLHNTQPSEPEVQESKKAESP
                     GIIDKEFFKKIGENFDPKKFNKKF"
                     /name="stbA"
     gene            complement(898..1866)
                     /locus_tag="NPH93_RS28050"
                     /old_locus_tag="NPH93_28035"
     CDS             complement(898..1866)
                     /locus_tag="NPH93_RS28050"
                     /old_locus_tag="NPH93_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_020956880.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFTLMNVPLR
                     CPDYTSVSKRAKSVNVSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSNTHEIICANLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTQLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRLTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
ORIGIN
        1 tgcgatagcg tgatttatca cgctgcgtta ggtgtatagc aggttaaggg ataaaaaatc
       61 atcttttttt ggtagggcga tctccgtagg ttaaggggca tttggctaaa aagcgtccta
      121 ttctttgatg gtcatgcttg catgaccatc tgagcaacca aaaactacag ataaactaca
      181 gataaactac aaaaaacgtt ttgccttagt gttgtaagac tacaaataga ctacaataaa
      241 actacaaata aactacaaaa aaacgtgaca gactacaaat agactacaat aaaactacag
      301 ataaactaca aaactcgatt gaccccttct tacgagtgtt gtagagtcat tttcatacaa
      361 cggagggggt tatgaataaa ccagcagatc tgaaaccccg caacttatcg gctgctgtca
      421 gattgcgcct aaatgaaatc gagaactggc tggacagagg gctaacgcgg catgaaattg
      481 ctgaaatcct cgacagcgaa tacagctttt cggtaacagc caaagggctt gagatggcac
      541 tgtatagaac gcggcaaaac cgaaaaaatg tattgcacaa tacgcatgat aagagtagcg
      601 cgaagggtgc agcggaaagt gtattgcaca atacacaacc gtctgagcct gaagtgcagg
      661 aaagtaaaaa agcagagagt cccggcatta ttgataaaga attcttcaag aaaatcggtg
      721 agaatttcga ccctaagaag ttcaacaaaa aattctgagg tgatttatga aagtagcggt
      781 aattaattac agtggcagtg ttcgtaaaac cttaatttca tcttacctgt tagccccgcg
      841 cggctttgtt gaataaatca gatttcgggt aagtctcccc cgtagcgggt tgtgttttca
      901 ggcaatacgc acgctttcag gcatacctgc tttcgtcatt ttgttcagcg ctcgtaccag
      961 ggccatagcc tctgcaacct gaccatcgta gtcacgcagt gtcagtgaac ctccgaacag
     1021 ctgttttacc cggtacatcg ccgtttccgc tatcgagcga cggttgtaat ctgttgtcca
     1081 tttccaccgc gcattactcc cggtcagccg ctgattcgca acagcacggt tacggtctgc
     1141 atattcaccg ggccagtaac ccgcgccttt tcggggcggg ataagcgcgc tgattttctt
     1201 acgccgcagt tcatcgtgac agagttgggt gtcgtaagcg ccgtctgccg atgctgccct
     1261 gatttttctg tgagtctgcc ggataagacc cgggaaggct tctgagtccg tcacattgtt
     1321 cagcgacagg tttgcacaga tgatttcatg tgtgttgctg tcaactgcca gatgcaactt
     1381 tcgccatata cgacggcgtt cctggccatg ctttttgact ttccattcgc cttcaccaaa
     1441 gaccttcagc ccggtggaat caatcaccag atgcgcgatt tcaccccggg tgaacgtttt
     1501 gaaactgaca ttaaccgact ttgcgcgctt gctgacactg gtgtaatccg ggcagcgcaa
     1561 cggaacattc atcagtgtaa aaatggaatc aataaaaccc tgtgcagccc gcagggtcaa
     1621 cctgaacacg cgtttaatga ccagaacggt ggtgatggcg agatcagaat agcgctgagg
     1681 tcttccccgt gatgaaggcg ttgccgactc ataccaggcc tgaatagctt catcatccag
     1741 ccagaaagtt atggagccac ggttgatgag ggctttattg taggtgggcc agttggttat
     1801 tttgaacttt tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc
     1861 cttcaactca gcaaaagttc gatttattca acaaagccca ttcagtcctc aatttgtgca
     1921 ttttttaaac gaaaaaaggg tgtctaatac ggtgaaatca tagtattacc aaagtaataa
     1981 ataaaaagtt atttaaatca gtaatttaga tttgatttgt aatacctggg taataccgca
     2041 gcatggctaa attttgtttt ttttctttta aatcag
//
