LOCUS       NZ_CP101840             4319 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Klebsiella pneumoniae strain GN4549 plasmid pGN4549-5, complete
            sequence.
ACCESSION   NZ_CP101840
VERSION     NZ_CP101840.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29935323
            Assembly: GCF_029201465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Melano,R.G. and Seah,C.
  TITLE     Detection and prevalence of carbapenemase-producing uropathogenic
            Enterobacteriaceae isolated from patients in a tertiary care
            hospital in Jamaica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Melano,R.G. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029201465.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 05:34:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,613
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 52 of 132
            Pseudo Genes (incomplete)         :: 81 of 132
            Pseudo Genes (internal stop)      :: 15 of 132
            Pseudo Genes (multiple problems)  :: 15 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101840.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GN4549"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pGN4549-5"
                     /geo_loc_name="Jamaica"
                     /lat_lon="18.1096 N 77.2975 W"
                     /collection_date="2021-06"
                     /collected_by="Camille-Ann Thoms-Rodriguez"
     gene            complement(157..372)
                     /locus_tag="NPH93_RS28010"
                     /old_locus_tag="NPH93_27995"
     CDS             complement(157..372)
                     /locus_tag="NPH93_RS28010"
                     /old_locus_tag="NPH93_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_047662815.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALNERVTDLSRQVQSLSEQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(379..876)
                     /locus_tag="NPH93_RS28015"
                     /old_locus_tag="NPH93_28000"
     CDS             complement(379..876)
                     /locus_tag="NPH93_RS28015"
                     /old_locus_tag="NPH93_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_167787817.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRTLSSAMSQRTKGMVRMVSQTWLTIVLVSVLLIASGAGILWWQGQQIL
                     DNYMSIREQKDALEKLNARTWGVRYQEDSQGRFLVLPEGVKADTNWTFDNGRKNGIRLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            complement(<911..1615)
                     /locus_tag="NPH93_RS28075"
                     /pseudo=""
     CDS             complement(<911..1615)
                     /locus_tag="NPH93_RS28075"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1554..1877)
                     /locus_tag="NPH93_RS28025"
                     /old_locus_tag="NPH93_28010"
     CDS             complement(1554..1877)
                     /locus_tag="NPH93_RS28025"
                     /old_locus_tag="NPH93_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_084558130.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAALMAIDAGLERLRQAVLEKGED
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2881..3342)
                     /locus_tag="NPH93_RS28030"
                     /old_locus_tag="NPH93_28015"
     CDS             complement(2881..3342)
                     /locus_tag="NPH93_RS28030"
                     /old_locus_tag="NPH93_28015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047662819.1"
                     /translation="MTSGSMSGCDFLAIPQVQHSEDSGVWRYVMEAGLGAEGILDISCL
                     EILEKDDGYLSFYHSDRMPKSDFISKVKHIHKLISLTKTNPAIYLNFDVIEAKESTEDR
                     TYFFDAGYPHIGMYYPEESDLNSLELRTILVEISEVYQRAYGGVVTKIL"
                     /name="hypothetical protein"
     gene            complement(3346..4053)
                     /locus_tag="NPH93_RS28035"
                     /old_locus_tag="NPH93_28020"
     CDS             complement(3346..4053)
                     /locus_tag="NPH93_RS28035"
                     /old_locus_tag="NPH93_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047662818.1"
                     /translation="MKPDNVILTVDRTKEQLFYRETTYEITPHELEDKGSVYFSVNKNS
                     FVSWGEWNSCLEVYKVTKPGEEYPAVLEALKINELSTPSPSFELVCEGFNHIADTLSEG
                     KQEEVKKFLGYVKNAVSHYKSKKDESQEELTPVKLYSSAKSFIEFMRYIPAFYNKLGIK
                     VYIDDRTGFFGVIYKRTSFNAGTLNILVKDNHEIDFSFAKRRKGVVTITGVAKFGKHLD
                     NSDQIRSVFKLME"
                     /name="hypothetical protein"
     gene            complement(4055..4318)
                     /locus_tag="NPH93_RS28040"
                     /old_locus_tag="NPH93_28025"
     CDS             complement(4055..4318)
                     /locus_tag="NPH93_RS28040"
                     /old_locus_tag="NPH93_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_047662817.1"
                     /translation="MNRKKTNGGNMAGKVNKVEHKEPARLQPPAFPLGMRFGFARDMCI
                     LDFLDQQNNGGGRYCFYSVAITKQHAIEMVNNLTKFIESQGD"
                     /name="hypothetical protein"
     Typing          complement(1991..2168)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.845"
                     /coverage="83.0"
                     /evalue="9.68e-52"
     Typing          2145..2629
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.762"
                     /coverage="93.0"
                     /evalue="1.01e-149"
     Typing          complement(54..1564)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.492"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1561..1877)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="3.23e-61"
ORIGIN
        1 actcctccat tacttttcta atataacata ctgcataaat atactattaa cctagagttg
       61 gaccgttgtg ccttctctgc ttatgtgaat actcaacttc tagcgatttt ctgtgttgaa
      121 cttccttttc ttgttgttgt tgccactcaa tagcattcag ccgctcgaca accggcgcag
      181 ctgctcgctc aaactctgca cctgcctgct caagtccgta acgcgctcgt tcagtgccgc
      241 gttctcccgt tgcataagcc cagacatcgt ccgccattcc gcgaaggcgt tctcccactc
      301 gtccagcctt ttcgagtagt cctgctgtag ctgctctaat gcgctcagca actgtttttc
      361 cagctccgtc atgctgctct actcctgcac cagtctgatg ccgttcttcc tgccgttgtc
      421 gaacgtccag ttcgtgtctg ccttcacgcc ttccggcagc accaggaagc gcccctggct
      481 gtcttcctgg tatctcacgc cccaggtccg ggcgttcagc ttctccagcg cgtccttctg
      541 ctcccggata ctcatgtaat tgtcgagtat ctgctgcccc tgccaccaca gaatgcccgc
      601 gcccgaggcg atcagcagca cggagaccag gacgatggtc agccacgtct ggctgaccat
      661 gcgcaccatg cctttcgtgc gctggctcat ggctgaggac agtgtccggt cgtggtcgag
      721 gatggcggcg ctgattctct tcgcgctttc gttcagttcc gcttttacag atttctcgtg
      781 ctcgctgaat gcggctttca gcatctcgcc ggtattctgc tgctgcgctt tcgatttctg
      841 ctctaagtcc ttcgccagcg ttaaaagact gttcataaat ggctcccttc agtcggatgt
      901 ttcgcccccc gtccgggtcg gcaatgctga tactgctttt tgtggtgcgg accacctcaa
      961 aacctgcgct ttccagcgcc tcagtgacgt cctgacgggt tttcagctcc ccggatgagg
     1021 caagagcaag caaaccgcgt gtaatcgctt gtgcggcctc ctgtttcgct tccggcagcg
     1081 cagatggcgt caccagcgcc cgtcggtttt ccggcgcgtt cgggtcgtgc agccccagcc
     1141 tgccgttcac tatggtctgc caggcatcaa tgcgcggacg gtcggcccgg tcgtaatacg
     1201 gctggagccg tttgccggtc agcagctcca tattcgggat caggaagttc agctccagcc
     1261 gccccttgtc ccggtgctca acccacagca cgctatactg gtctttatcg agtccgggca
     1321 tcagaacccg ctcaaagctc gccatcagct tttcacgctg gccggggggc aggtcttgtt
     1381 cggcaaagga caggacgccg gatgtgtact tttttgcgta gggtgaggcg tcgataagct
     1441 cccggacttc ctccggcttt ccctgcagga cgctggcgcc gtcgcgctgc cgatctttcc
     1501 ccagaagata atccaccgga ccggcgccgc cgccgcgccc tctgggatga aacttaacga
     1561 tcatcgtctt cccccttttc cagcacggcc tgccgcagcc gctcaagtcc ggcatcaatg
     1621 gccatcaacg cggcgacaac ctgcacgcga tcgtgcccgg taccgccacc ggcgttaacc
     1681 tgcctggcaa tctgattaag gttgttaccc atgccggcaa gctgacgaag cagcgccggc
     1741 gagatggatg gaagtttgcc agcacgcgag ggcttttcgt ccaggcatgt ctggcgcatc
     1801 caggccgcga gttgtttacc ctcacagcgc tccagaagtc ggcggtgctc gtcttctgtc
     1861 acccacatag tgagcatttt gttgcgcttt tctgccaaac catcctcctg ataacaccgg
     1921 ttcgggcggg aaattttttc ctgtccggag cggtgttggg caagcccgca gggcgcggca
     1981 gtgcttttca gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg
     2041 tatactggct caatatgtta cggcggagtg gtaatttatg aaagtgctct atgaggcagg
     2101 agaaaaaggt tgctcccgga tgaaagtgca atatgccgat gaaggtgaaa ttacgcctcc
     2161 tcgctcactc ggtcgctacg ctcctgccgt gagactgcgg cgggcgttac cagctcacag
     2221 ataacgtggt aagcgagatg ccgtcaaatc aagaaaggcc ggaaaaggat acggcgttgc
     2281 cgttttttca taggctccgc ccccctgatg aacatcacaa tatatgacgc tcaaatcagt
     2341 ggtggcgaaa cccgacagga cttaaagatc ccaggcgttt ccccctggta gctccctcgt
     2401 gcgctctcct gttcctgcca ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     2461 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     2521 tgcacgaacc ccccgttcag tccgactacc acgctctttt cggtaactat caacttgagt
     2581 ccaacccgaa aaggcacgac aaatcgccat tggcggtagc catcggtaat ggtttaaaca
     2641 gggagctgat cagaaaatag attaaaaggt ttcttaatct atcgaattgg agttcttgaa
     2701 gtgaatgcca gttagaggct acactagaag tacatttctg gtgactgcgc tccctataag
     2761 ccagttacct cggttcaaaa agttggtagc ttttagaacc tctgaaaaac caccgttgtc
     2821 gcggtggttt ttctttttcg aggatacatc tacaaaataa accgagtcaa ggattgggct
     2881 ttacaatatt ttagttacta ccccgccata ggctctctga taaacctcag aaatttccac
     2941 taatatggtt cttaattcga gcgaatttaa gtcactttct tcaggataat acatccctat
     3001 atgaggataa cctgcatcaa agaaataagt ccgatcctca gttgattctt tggcctcaat
     3061 gacgtcgaag ttcaaataga tagctgggtt ggtttttgtt aagcttataa gtttgtgtat
     3121 gtgtttgact ttactgataa aatcagactt aggcattctg tctgagtgat agaaagacaa
     3181 atatccatcg tctttctcaa gtatttctaa acatgaaata tccaaaatac cttcagcgcc
     3241 aagcccggct tccataacat acctccacac cccgctgtct tcgctgtgtt gaacttgagg
     3301 tatagccaaa aagtcgcatc cgctcattga gccagaagtc ataaatcact ccatgagttt
     3361 aaacactgat cttatttgat ctgaattatc taagtgctta ccaaattttg ccactcctgt
     3421 tatagtgaca acccctttcc ttctctttgc gaaagaaaaa tcaatttcat gattatcttt
     3481 cactagtatg tttagcgtgc ctgcattaaa tgatgttctc ttataaataa caccaaaaaa
     3541 accagtccta tcatctatat atactttaat ccccaattta ttataaaagg cgggaatata
     3601 tctcatgaat tcgataaaag atttcgctga ggaatataac ttaacaggag ttaattcttc
     3661 ttgagactca tcctttttgc ttttgtaatg agaaactgcg ttttttacat aaccaaggaa
     3721 tttttttact tcctcttgct taccctctga aagggtgtca gctatgtgat taaacccctc
     3781 acaaaccagt tcaaaagacg gggatggcgt tgataactca ttgattttaa gtgcctctag
     3841 aacagcaggg tattcttcgc ccggttttgt cactttgtag acttctaagc aagaattcca
     3901 ttcgccccag ctgacaaaac tatttttatt tacgctaaag tataccgacc ctttatcctc
     3961 tagttcatga ggagttattt cgtacgtcgt ttctcgatag aacaactgtt ccttggttct
     4021 atctaccgtc aaaataacat tatcgggttt catcttagtc gccttgtgat tctataaatt
     4081 tcgttaagtt attaaccatt tctattgcgt gttgttttgt tatcgcaaca gaatagaaac
     4141 agtatcggcc gccaccatta ttttgttgat ctaaaaaatc aagaatacac atatccctag
     4201 caaacccaaa tctcatacct aaaggaaacg caggtggttg cagccttgcc ggttctttat
     4261 gctctacttt attaacttta ccagccatat ttcctccatt cgtttttttt ctgttcata
//
