LOCUS       NZ_CP101839             9294 bp    DNA     circular CON 12-MAY-2024
DEFINITION  Klebsiella pneumoniae strain GN4549 plasmid pGN4549-4, complete
            sequence.
ACCESSION   NZ_CP101839
VERSION     NZ_CP101839.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29935323
            Assembly: GCF_029201465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Melano,R.G. and Seah,C.
  TITLE     Detection and prevalence of carbapenemase-producing uropathogenic
            Enterobacteriaceae isolated from patients in a tertiary care
            hospital in Jamaica
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Melano,R.G. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2022) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029201465.1-RS_2024_05_12
            Annotation Date                   :: 05/12/2024 05:34:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,613
            CDSs (total)                      :: 5,491
            Genes (coding)                    :: 5,359
            CDSs (with protein)               :: 5,359
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 52 of 132
            Pseudo Genes (incomplete)         :: 81 of 132
            Pseudo Genes (internal stop)      :: 15 of 132
            Pseudo Genes (multiple problems)  :: 15 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="GN4549"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pGN4549-4"
                     /geo_loc_name="Jamaica"
                     /lat_lon="18.1096 N 77.2975 W"
                     /collection_date="2021-06"
                     /collected_by="Camille-Ann Thoms-Rodriguez"
     gene            join(7834..9294,1..504)
                     /locus_tag="NPH93_RS27955"
                     /old_locus_tag="NPH93_27940"
     CDS             join(7834..9294,1..504)
                     /locus_tag="NPH93_RS27955"
                     /old_locus_tag="NPH93_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            504..1235
                     /locus_tag="NPH93_RS27960"
                     /old_locus_tag="NPH93_27945"
     CDS             504..1235
                     /locus_tag="NPH93_RS27960"
                     /old_locus_tag="NPH93_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            1242..1772
                     /locus_tag="NPH93_RS27965"
                     /old_locus_tag="NPH93_27950"
     CDS             1242..1772
                     /locus_tag="NPH93_RS27965"
                     /old_locus_tag="NPH93_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1800..1979)
                     /locus_tag="NPH93_RS27970"
                     /old_locus_tag="NPH93_27955"
     CDS             complement(1800..1979)
                     /locus_tag="NPH93_RS27970"
                     /old_locus_tag="NPH93_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2048..2425
                     /locus_tag="NPH93_RS27975"
                     /old_locus_tag="NPH93_27960"
     CDS             2048..2425
                     /locus_tag="NPH93_RS27975"
                     /old_locus_tag="NPH93_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(2487..2786)
                     /locus_tag="NPH93_RS27980"
                     /old_locus_tag="NPH93_27965"
     CDS             complement(2487..2786)
                     /locus_tag="NPH93_RS27980"
                     /old_locus_tag="NPH93_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2776..3039)
                     /locus_tag="NPH93_RS27985"
                     /old_locus_tag="NPH93_27970"
     CDS             complement(2776..3039)
                     /locus_tag="NPH93_RS27985"
                     /old_locus_tag="NPH93_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(3345..3764)
                     /locus_tag="NPH93_RS27990"
                     /old_locus_tag="NPH93_27975"
     CDS             complement(3345..3764)
                     /locus_tag="NPH93_RS27990"
                     /old_locus_tag="NPH93_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            4167..5852
                     /locus_tag="NPH93_RS27995"
                     /old_locus_tag="NPH93_27980"
     CDS             4167..5852
                     /locus_tag="NPH93_RS27995"
                     /old_locus_tag="NPH93_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5862..6119
                     /locus_tag="NPH93_RS28000"
                     /old_locus_tag="NPH93_27985"
     CDS             5862..6119
                     /locus_tag="NPH93_RS28000"
                     /old_locus_tag="NPH93_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            6204..6353
                     /locus_tag="NPH93_RS28005"
                     /old_locus_tag="NPH93_27990"
     CDS             6204..6353
                     /locus_tag="NPH93_RS28005"
                     /old_locus_tag="NPH93_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6457..7035)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          505..1232
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 ctgcgcagcc gggactgcaa tctgttgctc gcgcaccagt cgctgggcga tttcgggcag
       61 tgcgggcagg atctgcccgc tgaatttgtg aaaaccacgg tactggataa cacgccgatc
      121 cgctggtttt accgggcggc cagcctggag tccgcgcagt gggcggcggg gcagaccggg
      181 gaaatcaggg tggacgtgga gcgccgccgt gccagccggg aggcggggaa cgtggagcat
      241 atcagcgggg acagcttcat ccagaaggag gcgcggccgc tgtttgacgt gaacaccctg
      301 caacacctgc cggacgggtt cgcggtcatg accgggctgg gtgtggcccg gatggcgttc
      361 agcagtccgc tgcgggttga gcgccgggaa atcccgttgc agcgtttccc ggtgctggcg
      421 aaaaccgatc ccctggcgga gttccggccc ccggccgacg gcggcgggcc gaaagcggat
      481 gatgattttg cggggattta ctgatggcgc tggagctgta caacgtcagc cacgcgaaac
      541 gacaggcgcg gaacgcggag aaaacccggc tgaccctgcg ctggttgcgg gaagagctgt
      601 gttccacggc ggagctggtt gcgcggcggc tggggattgc ggcggtgcag ccggtctacc
      661 gcttcctcga cagcctggtg gcgaaagggc tgctggtccg gaccaaatat ccggtggacg
      721 gccgtcaggt cagcgtgtgg gggctgacgc cgcatggcgt ggcgttctcg ttcgacgagg
      781 acgaaccgct ggcggatgtt attccgtttc agccgtcaag ggtgtcggca gcacatctgc
      841 cgcaccggct ggccgtgcaa tccctgcgcc ttgatatgga agccagaggg gccaccggct
      901 ggcgcattct gcaccgtctg agtcttaaag gcatgaaagt gccggacgcc ctggcggagc
      961 tggacggtaa aacggtggcg tttgaggtgg agcgcaccgt caagagccgt cgccggtatc
     1021 aggaagtggt gtccgggtat ctgttcaacc gcaaagctaa cggcatcgat gaaatctggt
     1081 acatctgccc ggaccgggcg acgcaggtca gggtgcagcg ggcaatcctg tcggtggatg
     1141 aaatcgtgaa cccacagacc ggcgaggccc gcaaaacggc agagctggat cgtgaacggt
     1201 tgtttgcgtg ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat
     1261 ttattgaaga cgcggaggac cggaacggcg agcgctgcat catgccgacc agcgagcatc
     1321 tggaagatat ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc
     1381 gggcccagca tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg
     1441 acgaaaaagc cacgtacttt ttcactggta aaaatccgca atgggaacgg aacaaaccca
     1501 caggaatgaa gctgtgtgat tacctctatc gcaaggtgga gcggttcgcc atcgagaacg
     1561 gtgtgcaggt ggttgatgcc gggaagtgct caccggagca gattaacgcg atgattgacg
     1621 tggtgagtga gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc
     1681 gggaatataa cagccgctat tttaacgtgc cggtacctga actgccggag gacaaacctg
     1741 cccgccggtg gtggccgttc ggcaaaaaat aaatgccagc accggccatg ccggtgctgt
     1801 tattcctccc cgagcttatc ccgcatcacc tgccagagtg tttccgcatc ctcgtgtagc
     1861 cgctcgcaca tgtcggccgg ggtatccata tccagctcat tcagtttatc gagcaatagc
     1921 agcgactgct ccttaataaa tttagccata ttgagggctg ttttttcctg ttttgtcatg
     1981 attctttcct tccgggcatg gtgtgcgcct gtcaggtata ctgtctcaaa aatagcgggg
     2041 tgagacaatg gcgtgggtga cggtgagaca ggcgacggaa ctgacgggta aagcccgaag
     2101 ttccctgtat cgggatatgg cgaaaggccg cgtatcatac cgtacggaag ctgatggcgg
     2161 ccgggtggtg gataccagcg agcttatccg ggtgtacggt gagttaagac aggatgagac
     2221 tcacgagcgg gacgagttga gactgtcggg tgagactgaa aaaatggcct ccgacgcgtt
     2281 gatcgcggag ataaaagcac tcagggagga ggtggccggg ctacggcagg aaatgcagtc
     2341 catgagactg ctggaacaca aaccggagac agcagagacc ccggccggaa caccgcgccg
     2401 gtggtggccg tggggaaaac gctgacgcgc tgtgcttgtc caaccggcga cggccacgca
     2461 atgaattgcg tccccgtcac cggttattag agaacaatat ggggattctg gcgctggccg
     2521 accacggcca gaacccggac ttttctggtc tggtcatcaa catcatagag gacatgctgt
     2581 cccatcagac tgatccggcg aacgtcctcg ccatagcgtg agtccacttc atagagtcgc
     2641 gggaagtcgg ccagtttgtc cgccgcagcg agaacccggt cggtaacgtt ctgcgcatcc
     2701 cacagggatg aattttccgc gatatagtca aacagcgtcg ttacatcctc gatggctccc
     2761 tgcttccatt ccacgtcata cggcatcgcg cagttcctga taacggccat gcaggcgctg
     2821 gcggagttgt tccgtggtca tacccggacg cgtgtcagca cgggcggcgg tcactttggc
     2881 gcgaacctga tcttcatact ctgcctgggc cagttcttcg ttagccagtc gctgcaacag
     2941 cgcagctttg tccgggccac cttccggcag tatgcggggc aggctttcag ccagttcccg
     3001 aagaaagttg gcgttttcgt agtgtgtagt gttgctcatt gcggagcctc ccgggttaat
     3061 gcggcaatat gaaggaagtt tagcaggggg cggtctgaaa gaatatggtc gtgtcagtca
     3121 ggctgatgaa gcagaaagtc ggtaaaaaag ggcttacggc atccatttta cgtcaaaaca
     3181 tatgccatgc agttgcgtgc ataggcatgc attaggataa aatttatcgg gcgcgtttcc
     3241 ggcagttttc cgggggggtt gttgcctgtt ttatcccgta gccgccggaa acgccctgag
     3301 cccgtctgag cggtgcgcgt aatgacgcgt tatggtaaat ccacttactg tctgccttca
     3361 tagccatcga gataaacagc tgaaatggtg tcagccagta gccttgattt tttcggtgtg
     3421 aggtctttgc tgcggtaatc ggtacccgca aattgtgttg tgccttggag tgagtttaaa
     3481 aattcctgac tgtgaaactg ctgaatgcgt tgctgcacat cactccgtga ttttccggtc
     3541 gctttatcct ttgcgcccat atcccagaac tgccggaaaa aaggcaccat cattcgtgtt
     3601 gtgtcagctt tctgggtgga aaaattggga tcaaaaccgt catctgaagc atatacgtaa
     3661 tgccgggtat gctgttccac gctgcccttt ggagcacagg cagtgagagt tgctgtcgct
     3721 gttatcatgg cgataagaca cagtttgctg ccggaacgtc tcatgagcgt ctccttacct
     3781 ttaattcttt tttctcattg agttatatat ccgcaggcta tcttatcatc agtttctgac
     3841 cgtgttcagt gaccgaacaa cccgaaccag accccccgtt tcttttcctg tcccggaggg
     3901 ggctttctgg tatctcatat aatgatttca gtaaaacata ctgaggccgg agaacggcgc
     3961 cgtttgtgct ggtttttgtt gcatctggag gggattgcgc atggtgtgag tggctgtttt
     4021 tttgatcatt ttcacaaaaa cgcaaattta cagacttgac atgaaaaaca aggcagcgta
     4081 atttttatac tgtatataaa accagtggtt atatgtacag tattttattt tagtttattg
     4141 ttttaaaagt caaagaggaa aagtatatga gtggcggaga cggacgaggt ccgggtaatt
     4201 caggtctggg acacaatggt ggtcaggcca gtgggaatgt gaacggtaca tcaggtaaag
     4261 gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc
     4321 atacacctaa cggggatatt cataactata acccggggga gtttggtaac ggcgggagta
     4381 aacccggtgg taatggcggt aacagcggca atcatagcgg gagctctggt ggcggacagt
     4441 cttcggccac cgcgatggcc ttcggtctgc ctgccttggc cactccgggg gctgaaggac
     4501 tggctttatc cgtttccggc gatgcgttgt cggccgctgt tgctgatgtg ctggctgccc
     4561 tgaaaggtcc gtttaagttt ggtctgtggg ggattgcgat ttacggagtg ctgccttctg
     4621 agatagcaaa agatgatccg aaaatgatgt caaaaatcgt gacgtcattg ccggcagaca
     4681 cggtaacgga gacgccggtc agctccctgc cgctggacca ggcgacggtc agcgtcacta
     4741 aacgtgtggc ggatattgtg aaggacgagc ggcagcatat tgcggttgtc accggccggc
     4801 caatgagtgt tcctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt
     4861 cgattccggg tctcccgtct ctgcaggtga gcgtacctaa aggtgttccg gccgcgaaag
     4921 ccccgccaaa aggcattatt gctgaaaaag gtgattcacg tccggctggt tttacggccg
     4981 gtggtaactc ccgtgaagcc gttattcgtt tcccgaaaga gagcggacag aaaccggtct
     5041 atgtgtcggt gacggatgtt cttactccgg cacaggtaaa acagcgtctg gaggaagaaa
     5101 agcgtcgcca gcaggcatgg gacgctgctc acccggaaga ggggctgaaa agagagtatg
     5161 ataaagcgaa agctgagctg gatgctgaag ataaaaatat tgcgacctta aacagtcgca
     5221 ttgcatcgac agagaaggcg atccccggtg caagggctgc tgttcaggaa gccgataaaa
     5281 aggtaaaaga ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat
     5341 atggctccgg gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg
     5401 agaaattaaa agctgcccag acatctttaa acgaaatgaa tgaatcctta tccagggata
     5461 aggctgcgct ttccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg
     5521 cagaaaataa attaaatgag gaaaagaaaa aacctcgtaa gggaactaaa gattacggcc
     5581 atgattattt tcctgatccc aagactgaag atattaaggg gttgggagag ttgaaagagg
     5641 gtaaacctaa aacccctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggtgata
     5701 aaaagcgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg taccgcgcca
     5761 gtgatggcga acacctcggg gcattcgatc caaaaacggg taagcaggtt aaagggccgg
     5821 atccaaaacg aaacattaaa aaatatcttt aagaggtaaa tatggggctt aaattacata
     5881 ttcattggtt tgataagaaa accgaagagt ttaaaggtgg tgaatactca aaagacttcg
     5941 gtgatgatgg ttctgtcatt gaaagtctgg ggatgccttt aaaggataat attaataatg
     6001 gttggtttga tgttgaaaaa tcatgggttt cgatattaca gccacacttt aaaaatgtaa
     6061 tcgatattag taaatttgat tactttgtat cctttgttta ccgggatggt aactggtaag
     6121 caaaagttat caaggatgat ttgaaataca gacttccggt gttcagggat gaataccggg
     6181 gtttttaaat acagggaagg tatatgaaaa aggtcaaggt tgtttttctt tttatattga
     6241 ttttttcagg ctttcttctg gtcgcatgtc aggcaaacta tatccgggat gttcagggtg
     6301 gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag tagaaaagat
     6361 caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg taaacgaaaa
     6421 aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg
     6481 gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg
     6541 gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt
     6601 accaatggct gctgccagtg gcgttttgcg tgcttttccg ggttggactc aagtgaacag
     6661 ttaccggata aggcgcagca gtcgggctga acggggggtt cttgcttaca gcccagcttg
     6721 gagcgaacga cctacaccga gccgagatac cagagtgtga gctatgagaa agcgccacac
     6781 ttcccgcaag ggagaaaggc ggaacaggta tccgggaaac ggcagggttg gaacaggaga
     6841 gcgcaagagg gagcgattca tcggaaacgg tgatgatctt taagtcctgt cgggtttcgc
     6901 cactcctgac tgattcatgg ttgagccacc ggctcccaca gatgcaccga aaaagcgtct
     6961 gtttatgtga actcagtcag gagggcggag cctatggaaa aacgccacct gcgtggcctt
     7021 gccgttttgc ctcacatgtt agtcccctgc ttattcacgg aatctgtggg taactttgca
     7081 tgtgtccgca gcgcccgccg cagtctcacg gccggagcgt agcgaccgag tgagcgagga
     7141 ggcgcaattt ccccggcacc tacaagacgg acaccggacg cgctgatgtg gcatattgtc
     7201 ccctgattaa gtctttatat tccctgataa taaattacca ggataggtga atcatatcgg
     7261 tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct tacggagaaa
     7321 tacaggtcat ccccgcgagg gcgtggctga cctcaggggg atatcccctg cttacgctgg
     7381 ctggacgcca ccgacgcacg gtctctcccc ctctttctgg ctccgtccgg ctggacgccg
     7441 gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaaatcc
     7501 acggtgctgg acgcacctgg cgaagcagca aagctgctcc tgattttgcg ggtggtcagc
     7561 cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc actggggtgc
     7621 cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg cgccttttcc
     7681 gcatggggcg gaaagaggct ggcctccgtg gtgaaagctg aacgcaatca ccacggacgt
     7741 gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg ctgcacatca
     7801 cgccggcaaa ccatcgtctg aaggagagag aacatgttta atacggattc gcttgcctgg
     7861 aagtggcaca ttccggagct ggcccggcag atttatccgt gggtcagctg gctgtaccag
     7921 gagtattacg gtctggccgg ggctttccta gttatcggcc tgtttttcgg tctggctgaa
     7981 ctttgggccg gacggatgcg ttttgtgggg ctgagtcata aggagcgacg ggagtatgcc
     8041 gatcgctata agcgcgataa taaacggcca gcgccatttt cggtgctggc ctcgctggat
     8101 tcgcccgggc ggatggtcag ggcacgtcga catgtctgga actacgcgta ctggctgacc
     8161 ggcgcggccg gtctggccct tctggggggg ctggttgtcg ccgtgtttat cgagagctgg
     8221 ggagaaaccg tcctgcgtcc ggacctctac gcaaacgcag attattacct gctgcagccg
     8281 gctaacctgt tcgggattgc ctggtgcgcg ggcgggctgg ctgtcgggat tgtgttggcg
     8341 cggctgctgg cgttcctgct gcttgacggt cattttgcgg ctgctgacga ggcggtgaac
     8401 gcgaaactga atcaggagag ccggcgcagc agccagcgga caggagagat gaccgacgtg
     8461 cgccacctgc atttcgggga gccggtaccg gttaatgcgc tggccgattt ttcctcggag
     8521 caggcccggc aggagcaggc ggtgtttctg ggtaaggatg agcagggcaa gccggtgctg
     8581 gttccgcgtg atacctggcg caagaccaac attcagatcc tcggcctgcc cgggagcggc
     8641 aagagcgtga tggccaccaa cgcgctcatt cgctgcgtac gcgatttcgg ggacgcggtg
     8701 gtgtacttcg accccaaagg ggacgcatgg gcgccgcacg tcttccgggc gcactgtccg
     8761 gacttcaccc tgctggatct gcggccgggg aaaccggcgc agctgaatct gttccgcgat
     8821 ctcgaccagt acgcgctgaa aaacctgctg attgccgggc ttaacctggg cgagaccggc
     8881 gacgtggcgg accactaccg catcagcgag cagaaagcgg cgaagctgat tgcggagcag
     8941 tttccgcagg gggcaaacat tcagcaggtg ctggcggcgg cgtacgcgct gccggaggcc
     9001 ctgaaaaagg acgtgaaggg gttgatcact aggctggaaa acgtcgctga cctgagcgtc
     9061 ctgcagacgg acagtggcat cgatgtggcg gggattatca acggcggcgg ctgcctgtac
     9121 gttatcggct cgatggacga cgaggcggtg atccgtgtgc aaaaaatgct gtttgcccgc
     9181 tgcgcgcaaa tcatcattgc gcgggacgag ttccgggagt ggccacacgt cagcgtgatg
     9241 ctcgatgaaa ttaaatatct gctgtcgaag tatgtactga acgcactggg gacg
//
