LOCUS       NZ_CP101781            11970 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Klebsiella pneumoniae strain hvKP340 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP101781
VERSION     NZ_CP101781.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29889747
            Assembly: GCF_024496165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Wang,W., Luo,Y., Zi,C., Yang,S., Zhang,J. and Zhao,Z.
  TITLE     Hypervirulent Klebsiella pneumoniae 340 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Wang,W., Luo,Y., Zi,C., Yang,S., Zhang,J. and Zhao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2022) Department of Microbiology and Immunology of
            Basical Medicine, Guangdong Medical University, No. 1, Xincheng
            Avenue, Songshan Lake, Dongguan City, Guangdong, Dongguan, Guangdong
            523808, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUN-2022
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:24:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,071
            CDSs (total)                      :: 5,950
            Genes (coding)                    :: 5,754
            CDSs (with protein)               :: 5,754
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 69 of 196
            Pseudo Genes (incomplete)         :: 134 of 196
            Pseudo Genes (internal stop)      :: 26 of 196
            Pseudo Genes (multiple problems)  :: 33 of 196
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101781.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="hvKP340"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Zhanjiang"
                     /collection_date="2022-04-16T16:25:10Z"
     gene            47..1732
                     /locus_tag="NPJ84_RS29055"
                     /old_locus_tag="NPJ84_29055"
     CDS             47..1732
                     /locus_tag="NPJ84_RS29055"
                     /old_locus_tag="NPJ84_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1742..1999
                     /locus_tag="NPJ84_RS29060"
                     /old_locus_tag="NPJ84_29060"
     CDS             1742..1999
                     /locus_tag="NPJ84_RS29060"
                     /old_locus_tag="NPJ84_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2084..2233
                     /locus_tag="NPJ84_RS29065"
                     /old_locus_tag="NPJ84_29065"
     CDS             2084..2233
                     /locus_tag="NPJ84_RS29065"
                     /old_locus_tag="NPJ84_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3717..5681
                     /locus_tag="NPJ84_RS29070"
                     /old_locus_tag="NPJ84_29070"
     CDS             3717..5681
                     /locus_tag="NPJ84_RS29070"
                     /old_locus_tag="NPJ84_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5681..6412
                     /gene="mobC"
                     /locus_tag="NPJ84_RS29075"
                     /old_locus_tag="NPJ84_29075"
     CDS             5681..6412
                     /gene="mobC"
                     /locus_tag="NPJ84_RS29075"
                     /old_locus_tag="NPJ84_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6419..6949
                     /locus_tag="NPJ84_RS29080"
                     /old_locus_tag="NPJ84_29080"
     CDS             6419..6949
                     /locus_tag="NPJ84_RS29080"
                     /old_locus_tag="NPJ84_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6976..7155)
                     /locus_tag="NPJ84_RS29085"
                     /old_locus_tag="NPJ84_29085"
     CDS             complement(6976..7155)
                     /locus_tag="NPJ84_RS29085"
                     /old_locus_tag="NPJ84_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7181..7609)
                     /locus_tag="NPJ84_RS29090"
                     /old_locus_tag="NPJ84_29090"
     CDS             complement(7181..7609)
                     /locus_tag="NPJ84_RS29090"
                     /old_locus_tag="NPJ84_29090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7773..8087)
                     /locus_tag="NPJ84_RS29095"
                     /old_locus_tag="NPJ84_29095"
     CDS             complement(7773..8087)
                     /locus_tag="NPJ84_RS29095"
                     /old_locus_tag="NPJ84_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8075..8353)
                     /locus_tag="NPJ84_RS29100"
                     /old_locus_tag="NPJ84_29100"
     CDS             complement(8075..8353)
                     /locus_tag="NPJ84_RS29100"
                     /old_locus_tag="NPJ84_29100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8528..8893)
                     /locus_tag="NPJ84_RS29105"
                     /old_locus_tag="NPJ84_29105"
     CDS             complement(8528..8893)
                     /locus_tag="NPJ84_RS29105"
                     /old_locus_tag="NPJ84_29105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8890..9261)
                     /gene="tolA"
                     /locus_tag="NPJ84_RS29110"
                     /old_locus_tag="NPJ84_29110"
     CDS             complement(8890..9261)
                     /gene="tolA"
                     /locus_tag="NPJ84_RS29110"
                     /old_locus_tag="NPJ84_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9535..9780)
                     /locus_tag="NPJ84_RS29115"
                     /old_locus_tag="NPJ84_29115"
     CDS             complement(9535..9780)
                     /locus_tag="NPJ84_RS29115"
                     /old_locus_tag="NPJ84_29115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            10425..11912
                     /gene="ltrA"
                     /locus_tag="NPJ84_RS29120"
                     /old_locus_tag="NPJ84_29120"
     CDS             10425..11912
                     /gene="ltrA"
                     /locus_tag="NPJ84_RS29120"
                     /old_locus_tag="NPJ84_29120"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..6732
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2337..2915)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5682..6409
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgttggctt taatttattg ttttaaatgt caaagaggaa aacggtatga gtggtggaga
       61 cggtcgaggt ccgggtaatt caggtctggg acataatggt ggtcaggcca gtgggaatgt
      121 gaacggtacg tctggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg
      181 gccgggctgg ggtacgacgc atacgccgaa tggagatatt cataactata atccggggga
      241 gtttggtaac ggcgggagta aacccggtgg taatggtggt aacagcggca atcatagcgg
      301 tagctctggt ggcggacagt cttcggccac tgcgatggcc ttcggtctgc ctgccctggc
      361 tactccgggg gctgaaggac tggctttatc agtttccggc gatgcgttgt cggccgccgt
      421 tgctgatgtg ctggctgccc tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat
      481 ctacggtgtg ctgccttctg agattgcaaa agatgatccg aaaatgatgt caaaaattat
      541 gacgtcatta ccggccgatg cggtgacgga gactccggca agtactttac cactggacca
      601 ggcgacggtt cgtgtcagac aacgggttgt ggatgtggtg aaggatgagc ggcagcatat
      661 tgcggttgtc gcaggtcggc caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg
      721 tccgggggta ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa
      781 aggtgttccg acagcgaaag ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg
      841 tccggctggt tttacagccg gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga
      901 gaccggacag aagccggttt atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa
      961 acagcgtcag gaggaagaaa agcgtcgcca gcaggcatgg gacgccgctc atccggaaga
     1021 ggggctgaaa agagactatg ataaagcgaa agctgagctg gatgccgaag ataaaaatat
     1081 tgcgacctta aacagccgca ttgcatcgac agagaaggcg ctccccggtg caagggctgc
     1141 tgttcaggaa gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgtgac
     1201 ttataacccg cctcatgaat atggctccgg gtggcaggat caggttcgct atcttgataa
     1261 ggatattcag aatcagaatg agaaattaaa agcggctcag gcatctttaa acgcaatgaa
     1321 tgaatcctta tccagagata aggctgcact taccggggcg atggagagcc ggaaacaaaa
     1381 ggagaaaaaa gcgaaggatg cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa
     1441 ggggactaaa gactacggcc atgattatca tccagtacct aaaacagaag acataaaggg
     1501 acttggagag ttaaagcgag gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg
     1561 ggcccgatgg tatggcgata aaaaacgtaa aatttatgaa tgggattccc agcacggtga
     1621 gcttgaaggg tatcgtgcca gtgatggcga acacctaggc gcatttgatc ccaaaacggg
     1681 taagcaggtt aaagggccag atccaaaacg gaacattaaa aaatatcttt gagaggtaaa
     1741 tatggggctt aaattacgtt tgacatggtt tgataagaaa actgaagagt ttaaagggga
     1801 agagtattct aaagactttg gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt
     1861 aaaggataat attaataatg gtgagtttga tttgaagaaa gaatgggttc ctttactaca
     1921 gccatttttt aaaaatcaaa taaaacctga tgtggataat tgttatatag cttttgatta
     1981 tcgggacggt aactggtaac aaaaaattat caaggatgat ttgaaatact gacttccggt
     2041 gttcagggat gaatgccgga gtctttaaat acagggaaag tatatgaaaa aggtaaagac
     2101 tatttttctt tttatattga ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta
     2161 tatccgggat gttcagggcg gaacggtggc accatcgtcc tcctctgaac tgacggggat
     2221 cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2281 ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt
     2341 tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct
     2401 tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta gctaaacaaa
     2461 tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttgcg tgctttcccg
     2521 ggttggactc aagtgaacag ttaccggata aggcgcagca gtcgggctga acggggagtt
     2581 cttgcttaca gcccagcttg gagcgaacga cctacaccga gccgagatac cagtgtgtga
     2641 gctatgagaa agcgccacac ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg
     2701 gcagggtcgg aacaggagag cgcaagaggg agcgacctac cggaaacggt ggggatcttt
     2761 aagtcctgtc gggtttcgcc cgtactgtca gattcatggt tgagcctcac ggctcccaca
     2821 gatgcaccga aaaagcgtct gtttatgtga actctggcag gagggcggag cctatggaaa
     2881 aacgccaccg gcgcggccct gccgttttgc ctcacatgtt agtcccctgc ttatccacgg
     2941 aatctgtggg taactttgta tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt
     3001 agcgaccgag tgagcgagga ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg
     3061 cgctgatgtg ggatattgtc ccctgattaa gtctttctat tccctgacga tcaattatca
     3121 ggataggtga accatgtcgg tttttcgctg aacattttac gttaacctat ctctgttgct
     3181 tttttctcct tgcggagaaa tgcaagtcat ccccgctggg gcgtggctga cttcagggga
     3241 aaagaccttg cttacgctgg ctggacgcca ccgacgcacg gtcttttccc ctctttctgg
     3301 ctctgtccgg ctggacgccg gccacgccag aaattcaccc ggcccccgtc aggctcacgc
     3361 ctgaccaccc acaaaaatcc acggtgctgg acgcacctga cgaagcagca aagctgctcc
     3421 tgattttgcg ggtggtcagc cgctcgctat ggcgaactct gacgggggcc ggaacaccgg
     3481 aaaagaccgc actggggtgc cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc
     3541 tccggggctg cgccttttcc gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg
     3601 aacgcaatca ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt
     3661 gcagcggggg ctgcgcatca cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt
     3721 ttaatacgga ttcgcttgcc tggcagtggc atatcccgga gctggcccgg cagatttacc
     3781 cgtgggtcag ctggctgtac cgggagtatt acagtctggc cggttttttt ctggttatcg
     3841 gcctgttttt cggtctggct gaactctggg ccggccgaat gcgttttgtg gggctgagcc
     3901 ataaggcgcg gcgggagtat gccgatcgct ataagcgcga taataaccgg actgtgccat
     3961 tttcggtgct ggcctcgctg gattcacccg ggcggatggt cagggcacgg cgacatgtct
     4021 ggaactacgc gtactggctg accggcgctg ccggtctggc ccttctgggg gggctggttg
     4081 tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg
     4141 cagattatta cctgctgcag ccggctaacc tgttcgggat agcctggtgt gccggagggc
     4201 ttgtcgccgg cgtccttctg gcgcggctgc tggcgttcct gctgcttgat ggtcattttg
     4261 cggccgctga cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc agcagccagc
     4321 ggaccggaga aatgacagac gtccgccacc tgcatttcgg ggagccggta ccggttaacg
     4381 cgctggccga tttttccacg gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg
     4441 atgagcaggg ccagccggtg ctggtccctc gtgatacctg gcgcaagacc aatattcaga
     4501 tcctcggcct gcccgggagc ggcaagagcg cgatggccac caacgcgctc attcgctgcg
     4561 tacgcgattt cggggacgct gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc
     4621 acgtcttccg ggcgcactgc ccggacttca ccctgctgga tctgcggccg gggaaaccgg
     4681 cgcagctgaa tctgttccgc gatctcgacc agtacgccct gaagaatctg ctggttgccg
     4741 ggtttaacct gagtgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag
     4801 cggcgaagct gattgcggag cagttcccgc agggggcaaa cattcagcag gtgctggcgg
     4861 cggcgtacgc gttgccggag gccctgaaaa aggacgtgaa ggggctgatc accaagctgg
     4921 agaacgtggc cgacctgagc gtcctgcaga cggacagcgg tatcgatgtg gcggggataa
     4981 tcaacggcgg cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg
     5041 tgcagaagat gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga
     5101 ggtggccgca cgcgagcatc atgctcgatg agattaaata tctgctgtcg aagtacgtgc
     5161 tgaacgcgct ggggacgctg cgcagccggg actgcaacct gctgctcgcg caccagtcgc
     5221 tgggcgactt cggtcagtgc gggcaggatc tgcccgctga ttttgtgaaa accacggtac
     5281 tggataacac gccgatccgc tggttctatc gcgcggccag ccaggagtcc gcgcagtggg
     5341 cggcgggcca gaccggggag atccgggtgg acgtggagcg ccgccgggcc agccgggagg
     5401 cggggaacgt ggagcacatc agcggcgaca gcttcatcca gaaggaggcg cggccgctgt
     5461 ttgacgtgaa caccctgcaa cacctgcctg acgggttcgc ggtgatgacc gggctgggcg
     5521 tggcgcggct ggcgttcagc agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa
     5581 ccttcccggt gctggcgaaa acggacccgc tggcggagta cctgcctccg gccgacggcg
     5641 gcggaccaaa agcggatgat gattttgcgg ggctttactg atgacactgg agcagtacaa
     5701 cgtcagccac gcgaagcggc aggcgcggaa cgcggagaaa acacggctga ccctgcgctg
     5761 gctgcgggaa gagctgtgtt ccacggcgga gctggttgcg cgacggctgg ggattgcggc
     5821 ggtacagccg gtttaccgct tcctggacag tctggtggcg aaagggctgc tggtccgggc
     5881 gaaatacccg gttgacggcc gtcaggtcag cgtgtggggg ctgacgccgc acggggtggc
     5941 gttctcgttc gacgaggacg agccgctgac ggacgttatt ccgtttcagc cgtcaagggt
     6001 gtcggcggcg cagctgccgc accggctggc cgtgcagtcc ctgcgcctct ccatggaggg
     6061 ccggggggcc accggctggc ggtacctgca ccgtatggcc ctgaaaggga tgaaagtgcc
     6121 ggacgcgctg gcggagctgg acggcagaac ggtggcattt gaggtagagc gcaccgtcaa
     6181 gagccgcagg cggtatcagg aagtggtgtc cgggtatctg ttcaaccgcc gggcgaacgg
     6241 catcgatgaa atctggtaca tctgccctga ccggccgacg cttgtccggg tacagcgggc
     6301 gattctggcg gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca aaacggtgga
     6361 gctggaccgg gagaggctgt tcgcgtgctt taaatttatg acaacggagt gagggattat
     6421 ggcgctgtac atggtattta ttgaggacgt ggaagacaaa gacggcgagc gctgcattat
     6481 gccaaccagc gagcatctgg aagacatggt ggtgctcgcc tggcagaccg gggcgtatgt
     6541 tttctgtttt gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga
     6601 cgagcggctc aacagcgacg aaaaagccac gtactttttc accggtgaaa atccgcaatg
     6661 ggaacgggac aaacccacag gaatgaagct gtgtgattac ctctatcgaa aggtggagcg
     6721 gttcgccatc gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat
     6781 taacgcgatg attgaggtgg tcagcgagcg gcacggggaa agcgcggtga acgagaagct
     6841 gaaagcggag ctgatgcggg aatataacag ccgctatttt aacgtgccgg tgcctgaact
     6901 gccggaggac aaacccgccc gccggtggtg gccgttcggc aaaaaataac gccagcaccg
     6961 gcatggccgg tgctgttatt cttccccgag cttatcccgc atcacctgcc agagtgtttc
     7021 cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta tccatgtcca gctcgttcag
     7081 cttttccagc aacagcagcg actgatcctt aatgaattta gccatattga gggcagtttt
     7141 ttcctgcttc gtcatgttct ccattccctt cggtaagttc tcagctaaac cagccggacc
     7201 agaagcgttt tttcttctgt gtcagttctg ttgtcaatct gtccacttcg gcctggagtt
     7261 tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta tcttccagca
     7321 tcagggtgac agcctgccgg agatcggcca gttccttttg cattgccaga agctgttcct
     7381 gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt ttctcaggga
     7441 tattcacatt tccgtataca cggatcagtt ctgacacatc cacgacaggg ttgtttttcc
     7501 cgtcacgtga cacggtaacc tttccctgtt ttacatgatt gtatagcgtt cttctggtaa
     7561 tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact gtatacccct
     7621 gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc tgaattttac
     7681 ccgtttttcc gttcaggatg tggttttctg acgcgctgcg cttgtcaaac cggcgacggc
     7741 cacgcaatga attgcgcccc cgtcatcggt tattagaggc tgtctgcccc cggatacccg
     7801 gattctttct gatgcctgac ggtgacgatc acaatctggt ccatttcccg gtcgtggcgg
     7861 tagagcatca catagccact gtcgccaaac ccgatcacca gttccttata ttccagcggc
     7921 agaaacggca ccgggcggcc aatgtctggc aatgttttca gttgctggat agcccggacg
     7981 atcacctcac ctccttttct ggctgccagc cgatttttgg ttttgagaaa gtcctgaagg
     8041 cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc ggagcatcct
     8101 gctcgttctc agttccccag gtacttatcc aggcttccgc ttcttccgca gtaaggtgca
     8161 gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac tggctgcgct
     8221 tctcttcccg gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc gctgagcggt
     8281 gccggtcatc ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc ctgatggatg
     8341 ttgctgatga cataaaagcc cctttgtagt catatgtatt acacatgact actttaatcc
     8401 gtggcggcgc tatttgccag aaatacggct tttgctgccc atttttcagc ttcgcttcca
     8461 ctaatcaggg ctgctacagg ctcactcatc atccaggact attgcagcct gcgggttaaa
     8521 ggacctgtta ctggcggcaa ccatcatcat tgagcttttg ggtggtgact ggtcgcggat
     8581 cctgtaagcc gtcatttttc agactgaatt caaaatttat aacctgtcgc attgccgggt
     8641 gccctgcggg atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa aaaaggtgac
     8701 agccggtaag cgacacgttg tccactcgtc catcgtccag aatatcaaac gtggcggtca
     8761 cctgaccttc gatgcgtttt gctgcggcct gtaccgggta ggttggctgc agtgtggtta
     8821 cgggttttag tggtatcgca tgtaccggag tgaggaacat ggccgccagc actgtcagag
     8881 taaaagcttt catggtctga aatccagagg ggcgtttttg aatatttgcc aggtattttc
     8941 atccggtgcc ggcggaattt tggcgtgggt aacggctgat attgctgctt tacagagttt
     9001 cggatctcct ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta tggatatccg
     9061 cacggtgcac atttttcccg cataggcctc tggttcaaac atctgcattt caacagcggt
     9121 cttgatggcc gcagcgtagc tactgatttt agatgactta tccagagtat aaattttcgg
     9181 cggctgggtg gtggaatgct tacaggccgt caggagtaag gggctgcaca atgccagcag
     9241 gataaagggt attaaacgca tcgggttgat gacctgtcag taaaaaatgt tacatgtata
     9301 tttgtcactc tttacggcca cgttcattta cataaacacg gcttatcgta tcgtttttac
     9361 accaaacggc ccgccatgca gttgcgtgca taggcatgca gcgggttaca atttaccggg
     9421 cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt tatcccgtag ccgccggaaa
     9481 cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata ccccttaccg
     9541 gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct cccgacgcat
     9601 ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc tttcccgcac
     9661 ctgttgtttt accgcctttg gcagcgtttt gttccctttc aggcccatgg ccagtgcctg
     9721 agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca ggtgcgtcat
     9781 agtggcctcc gggcgtctca ggtttcccga acagtttaac cgcaggtcct ctcctcatgc
     9841 cagttagctt tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa tgtcttaaat
     9901 aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt acaggtggct
     9961 ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca cctgaaaacg
    10021 ggaggagtaa ggtaataatt atactgtgta tatgtacagt gtgcgccggg agaccggtta
    10081 gagatcaagg ggtgaaagtc cccgaccatt gaaggaccag caatccacaa ggtccccgag
    10141 tcatgcgttg cataccgcga ggtatggggc gaagcgttga caggggtgtt gacaggccag
    10201 ccattgagcc acgaaatgta tattaaatta ccgggtgccg acgttgtact gttaacggaa
    10261 ggcaacatca tagggtgcga tactgcgagt gccacatgga cccggcgggg tctgagaccc
    10321 tggcatgtca atacgatctc tacgcgggaa ccgggagatc tcccctctga ccatctgcca
    10381 gtgtcggaga tggcccgcac cgggaagacg aggagtcata gccggtgatg tacggagagg
    10441 agaagtcgga ctcgctcata gtagcggcga agcaggcgaa caacccgaaa ggagcggagt
    10501 cagtggagcg aaggagcggg gccaagggga acgcggaaca gccacacatg cgccggacac
    10561 agagccggga aagcatgtca cagaggctgt cacgcgtgcg ggaagctgcg aagcagcgga
    10621 agaaagaacg gtttacagca ttgttccacc tgctgacagt cgaagcactg gaagccgcat
    10681 tcctctccct gagcaggaaa gcggccgccg gagtggatgg catcaggtgg atggactacg
    10741 ccggaaacat gaagaacaac ataacagatc tgcaccggag gctacatcag ggcagctaca
    10801 gggcgcagcc cggcaggcgt cactacatcc caaaagcgga tggaaaacaa cgcccgctcg
    10861 gcatcgcctc gctggaggac aagatcgtcc agtatgcgct ggtgaaaatc ctgaacgcag
    10921 tctatgaaaa cgactttatg gggttctcat acgggttcag acccgggcga agccagcacg
    10981 atgcactgga cgcactggcc acagggctgg tacgcactaa cgtaaactgg gtactggatg
    11041 ccgacatcag tcagttcttc gacagggtga gccacgaatg gctgatcagg ttcacagagc
    11101 atcggatcgg cgaccggagg gtaatcaggc tcatacgtaa gtggctcaca gccgggacgt
    11161 cggaggaggg tcaatggcga gcaacggagg aaggcacccc acagggtgcg gtcatctcac
    11221 cgctgctggc aaacatatac ctccactacg tcttcgatct gtgggcgcat cagtggcgac
    11281 gtcgctatgc cacaggcaat gtggtaatgg tcagatacgc cgatgacatc gtcatcgggt
    11341 tcgacaaacg atacgatgcc cggcgcttcc gtatagccat gcagcgcaga ctgagggagt
    11401 tcggactcac ggttcacccg gagaaaaccc gtctgatgga gttcggccgc ttcgctgccg
    11461 aaaaccgtgc catcagggga aaaggcaaac cagaaacgtt caacttcctc gggttcacgc
    11521 acatcagcgg gaaagatcgc aacggcaggt tcatgctgat acgaaagacc cgccgggatc
    11581 ggatgacggc aactctgaaa gccatcaaag acggtctgcg aaggcgctgg cattactcaa
    11641 tccccgaaca gggaaaatgg ctcaggagag tggttcaggg atacctgaac tatcactcgg
    11701 taccgggcaa cttccccacc atgcagaagt tcaggacaca cgtaacaaac ctctggcgcc
    11761 gggcgctcag gcgcaggagc cagaaggatg atacgacctg gacgaaagca aacaaactgg
    11821 cagccgcatg gctaccaagg gttcgggttc ttcatccatg gcctgtggag cggttcaccg
    11881 ccagacaccc gaggcaggag cccggtgcgt aaatcgcgca cgccgggatc tgtgcggggg
    11941 gtatccggta acgggtatcc ctaccgcgac
//
