LOCUS       NZ_CP101777             5596 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Klebsiella pneumoniae strain hvKP340 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP101777
VERSION     NZ_CP101777.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29889747
            Assembly: GCF_024496165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,W., Luo,Y., Zi,C., Yang,S., Zhang,J. and Zhao,Z.
  TITLE     Hypervirulent Klebsiella pneumoniae 340 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,W., Luo,Y., Zi,C., Yang,S., Zhang,J. and Zhao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2022) Department of Microbiology and Immunology of
            Basical Medicine, Guangdong Medical University, No. 1, Xincheng
            Avenue, Songshan Lake, Dongguan City, Guangdong, Dongguan, Guangdong
            523808, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUN-2022
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:24:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,071
            CDSs (total)                      :: 5,950
            Genes (coding)                    :: 5,754
            CDSs (with protein)               :: 5,754
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 196
            CDSs (without protein)            :: 196
            Pseudo Genes (ambiguous residues) :: 0 of 196
            Pseudo Genes (frameshifted)       :: 69 of 196
            Pseudo Genes (incomplete)         :: 134 of 196
            Pseudo Genes (internal stop)      :: 26 of 196
            Pseudo Genes (multiple problems)  :: 33 of 196
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101777.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="hvKP340"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="China:Zhanjiang"
                     /collection_date="2022-04-16T16:25:10Z"
     gene            complement(join(5433..5596,1..61))
                     /locus_tag="NPJ84_RS27980"
                     /old_locus_tag="NPJ84_27980"
     CDS             complement(join(5433..5596,1..61))
                     /locus_tag="NPJ84_RS27980"
                     /old_locus_tag="NPJ84_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            224..352
                     /locus_tag="NPJ84_RS27985"
                     /old_locus_tag="NPJ84_27985"
     CDS             224..352
                     /locus_tag="NPJ84_RS27985"
                     /old_locus_tag="NPJ84_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(728..940)
                     /locus_tag="NPJ84_RS27990"
                     /old_locus_tag="NPJ84_27990"
     CDS             complement(728..940)
                     /locus_tag="NPJ84_RS27990"
                     /old_locus_tag="NPJ84_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(947..1456)
                     /locus_tag="NPJ84_RS27995"
                     /old_locus_tag="NPJ84_27995"
     CDS             complement(947..1456)
                     /locus_tag="NPJ84_RS27995"
                     /old_locus_tag="NPJ84_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1491..1925)
                     /locus_tag="NPJ84_RS28000"
                     /old_locus_tag="NPJ84_28000"
                     /pseudo=""
     CDS             complement(<1491..1925)
                     /locus_tag="NPJ84_RS28000"
                     /old_locus_tag="NPJ84_28000"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2134..2478)
                     /locus_tag="NPJ84_RS28005"
                     /old_locus_tag="NPJ84_28005"
     CDS             complement(2134..2478)
                     /locus_tag="NPJ84_RS28005"
                     /old_locus_tag="NPJ84_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2468..2644)
                     /locus_tag="NPJ84_RS28010"
                     /old_locus_tag="NPJ84_28010"
     CDS             complement(2468..2644)
                     /locus_tag="NPJ84_RS28010"
                     /old_locus_tag="NPJ84_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3800..4126
                     /locus_tag="NPJ84_RS28015"
                     /old_locus_tag="NPJ84_28015"
     CDS             3800..4126
                     /locus_tag="NPJ84_RS28015"
                     /old_locus_tag="NPJ84_28015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4152..4697
                     /locus_tag="NPJ84_RS28020"
                     /old_locus_tag="NPJ84_28020"
     CDS             4152..4697
                     /locus_tag="NPJ84_RS28020"
                     /old_locus_tag="NPJ84_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            4763..5113
                     /locus_tag="NPJ84_RS28025"
                     /old_locus_tag="NPJ84_28025"
     CDS             4763..5113
                     /locus_tag="NPJ84_RS28025"
                     /old_locus_tag="NPJ84_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(5183..5449)
                     /locus_tag="NPJ84_RS28030"
                     /old_locus_tag="NPJ84_28030"
     CDS             complement(5183..5449)
                     /locus_tag="NPJ84_RS28030"
                     /old_locus_tag="NPJ84_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2731..2807)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          2996..3393
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(613..2144)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttgcttcgc cagcgagtcc agacgggact caatctcatc agataaccgg atagcaagca
       61 tataaacctc ccgaacacca tttgctatac atgtatatca acagtaccac aatcaaccta
      121 aaagaacaaa aaataatctt gttagcccct cttgataatc cgtaacgcgg tcaatatatc
      181 tagcttgagg gctttcctct taactattga aaggataacg attatgggtc ttaagccggg
      241 tccgaaacga attgccgatt caacagggga acctgataaa cgccagcgtg acaataaaaa
      301 aacgccggga aatactgaca aattgaaacc cagcaaatct tcaaaaaaat aagtcagtag
      361 ttaaaagccc tccggggctt ttttgtatct gccgttcacg ctgattaaac ataacacccg
      421 ttgcccgcac cgccccggac gggttcgccg cgaacggggt ggcagtcagg taaaaccggc
      481 aacaaaccac ccgataaacc gccccgttcg cgacagataa attttacctg gctgcatagc
      541 catgcagcca ggtgaatgac atccggtttc agcgtaaatg cccgatgaga accctgtttt
      601 cctgcatttc agcccagcga cggaccgtca tagcttcgct gccgggaacg cgcttccgtc
      661 agttcctttt cgtgctgccg ttgctcgtgc gcccgttctg cggccacggc catctcatga
      721 cgctgcacta ccggctcaaa cgctgcagtt gcccgctcaa gcgttccacc tgctgactca
      781 agcgcgtcac gcgctcgctc agcgccgcgt tctcccgttg cataagccct gacattttcc
      841 gccattcccc gaaggcgctc tcccactcgt ccagcctttt cgagtagtcc tgctgtagct
      901 gctctaatgc gctcagcaac tgtttttcca gctcggtcat cggcacttac tccttcacca
      961 gtctcagcgc gttctgcttc gaccgcccgt tcccgaccgt ccagctctgc gtcctgtcta
     1021 cggccgtccc cttcggcagc accagaaaac gtccgttgct gtcttccagg tacgtcacgc
     1081 cccaggtctt cgcgttcagc ttcgacagcg ctgcgttctg ccggtcgatt tcggccagat
     1141 ttgacgctat caagctcccc tgataccaca acaccccgct caggctggcg aacagcagtc
     1201 ctgacaccat cgtgatggtc agccatgtcc gcccgaccat acgcatcacg ctcaggcggt
     1261 tcgactgcat ggccgcgctc ataccctgct cgtgggcgcg gatggcggcg ctgattctct
     1321 tcccgctttc gctcagttcc gctctgacag acttctcgtg ctcgctgaat gcggctttca
     1381 gcatttcgcc ggtactctgc tgctgcgctt tcgatttctg ctctaagtcc ttcgccagcg
     1441 ttaaaagact gttcatagat ggctccctta agtcggatgt ttcgccctcc gtccgggtcg
     1501 gcaatgctga tgctgctctt tgtggtgcgc acgacctcaa aacctgcgct ttccagcgcc
     1561 tcagtgacgt cctgacgcgt tttaagctcc cctgacgagg cgagggccag taagccccgc
     1621 gtaatagcct ctgcggcttc ctgcttcgtt ttgggtaacg aagatggcgt caccagcgcg
     1681 cgccggttct ccggtgcgtt cgggtcgtgc agccccagtc gcccgttcac cacggtctgc
     1741 cacgcatcga tgcgcggacg gtctgcccgg tcgtagtacg gctggagacg cctgccggtc
     1801 agcagttccg tgttcggtac cagaaaattc agttccagcc gccccttgtc ccggtgttca
     1861 acccacagca cgctgtactg gtctttatcg agtccgggca tcagaacccg ctcgaagctc
     1921 gccatcagct tttcacgctg gccgggcggt aaatcctgtt cagcaaaaga caggacgccg
     1981 gaggtgtact ttttggcgta gggcgaggcg tcgataagct cccggacctc ctccggcttt
     2041 ccctgcagga cgatggcccc gtcgcgctgg cggtctttcc ccagcagata atccaccggc
     2101 ccggcaccgc cgcctcgccc tcgcggatga aacttaacga tcatcgtctg cccccttttc
     2161 cagcacggca tgccgcagcc gctcgagttc ggcatcaatg gccatcagcg cggccaccac
     2221 ctgaacccgg tcagcgcctg accactggcc gccgttaatt ttccgggcga tctggttgag
     2281 gttgttcccc atcccggcaa gctggcgcag caaaaccgga tcgatggacg gcagccgcaa
     2341 cgaccgcgcc gggcgcgtat cgagacaggt ttcccgcatc cacaccgcca gttgccttcc
     2401 attgcagcgc tccagcagct gctggtactc cccatcggtc acccggatac ctaaaaaacg
     2461 ggtacgctta accgccatcc tctcctccga tatccgcaaa atgtgaccgg gctgaacgct
     2521 caagctctgc catctgctcc cggcggtaat cttcctcctg tgcctgacgc atcagatttg
     2581 ccatcactgc cggatgccag gatctttttt ttacgacgag cggcttcttc ttcggcttct
     2641 gcattatccc tccttcgcaa acgccggacg gcacggaaaa gttttccggg tcgctccggc
     2701 gttggacaag cgccagcgcg tcagtggtgt tagcgggttt cggggcgcag ccctgaacca
     2761 gtcacagcgt ttacgctgtg tatactggct tactatgttg gcatgagagc acgattcatg
     2821 aaagtgcctc atgtggcagg tgtgatatgt ggctcaggag cgtccgcaat accatgcacg
     2881 gacagatgaa atgacgcctc ctcgctcact cggtagctgc gctcctgccg ttcggctgcg
     2941 gcgggcgtga ccgactcacg gataacggga taaagcaaag gcgctcaaat cagaaagggc
     3001 cggagaaaag gatgcggcgt tgccgttttt ccataggctc cgcctcccct gacgagcatc
     3061 acagaaatgg acgctcaagt cagaggagga cgaaacccga caggacttaa agatgccggt
     3121 gatttctctc cggcggctcc ctcgtgcgct ctcctgttcc gaccctgccg tttaccggat
     3181 acctgttccg cctttttccc tttcgggaag cgtggcgctt tctcatagct cacgctgctg
     3241 gtatctcggc tcggtttagg tcgttcgctc caagctgggc tgtatgcacg aaccccccgt
     3301 tcagcccgac tgctgcgccc tttccggtaa ctatcatctt gagtccaacc cggacggaca
     3361 cgcaaaaacg ccactggcag cagccattgg taactggata tatacgagag agatattgag
     3421 aggtcttgaa gtgggtgcct aactgcggct acactgaaag aacagttttg gtatctgcgg
     3481 tctcgtatac ccagttaccc ggttaagcag ttccccaact gacttaacct tcgatcaaac
     3541 cgcctcccca ggcggttttt tcgtttacag tgcaagagat tacgcgcaga aaaaaaggat
     3601 ctcaagaaga tcctttaaat gatcgtcagt tctgttatcc ccggagctcc atccatccca
     3661 cggaaaaaca cccgccactg atgccctcag cgcactgcac atcatgtaca aaccactccc
     3721 tccctccttt tttattgcgt ttcttaatgc gcaatggtag ctttgtgcgc aacaaaaaac
     3781 gcaatagcaa gggggggtaa tgagagaaag aacaaatatc atgctagata taggattaaa
     3841 gcaatcctta caacggcttt cgaataaaac caaaatgagt ctaagtgatc ttattaacca
     3901 gagattatca ggttcacttt cagttgaaga acgcattggc gaaaagcctg aatggatgag
     3961 aaaccttgat ccctatctcg ccaatcactt ttcaaatatt gagttaccgc tcccttcacc
     4021 attaaaagaa taccaagaaa aaatggatga gatagattca attattaatg acatgatggt
     4081 tctaaaggaa tatttcagag ctaagtttta cgacgaaata aaataggcac gctatacgga
     4141 ggcggagaat tatgtttttt aaacggtatt taacggagaa ggaaatatac aaggcagcac
     4201 tcccaattgt taggcttcaa gggagagtta acacttcatt acgtttttta tccaaatact
     4261 cagatgtaaa caaaaatgga ttaattgcat tgcatcttaa tttattacat tttatctcat
     4321 acaacataca cggtgtagag tctctgaaaa tcactcaagc aggcataaac aaagctgtac
     4381 aacaaagccc tcacgcagat gaaataaaaa atattgcgca ggcaatagga cgcaaaggac
     4441 ctgagtacat cgaaggatta attagaaacc catgggggaa gaaaaatgat aatccactaa
     4501 tatttataat gagcattgaa gaaagtataa aagaaaccct taactcaggt tatatgttat
     4561 atagagtagc tcccataact gagggtgaag cagctgcttt catggatgtt cttaaacaat
     4621 tattattatt tgaggataat tttcgccttt ttggtagaga cttggtttat aaaatagaaa
     4681 aattaaaaat cagataacaa agtatggtga acgaaaaatg aaagagagga tagtgaaatg
     4741 tgtaatacca agattctcaa ctatgtattg gttaattgcg ttcctagcat ttatattccc
     4801 cggtcgatct tttgctgacg gttacaccgc ttactaccga tgtaatggta ctcaggaagc
     4861 atctcttact gttaaagata acaaactaag tatggttgta ggtctttcta agtttaatag
     4921 gcaactggat acttatagat tcaaagaaaa gggaatcgaa gtagtaatgc agagagctgc
     4981 ttctgatgat aactctgagg ccataatgtc ttttattatg cctgacgtta acaatatgac
     5041 cttattagtt aaaaataacg gctacattat cgttaagaca acctgctggt taactaaaac
     5101 cgacaaacag tagaacacgt agttagtcga gtatttacca taatacctgt tgtcggcatc
     5161 aaaaaagccc gcatagcggg cttcagtcgt aaacttcgcg gcgatgtcca atcgtggcgg
     5221 caaggataac aagctcgtca tctcggatct cacacagaac gcgataatct cccacccgat
     5281 agcgccagaa ctcgcccagc tcacctttga gcggcttccc tgactggcga ggatcggcag
     5341 caactgcaat gcgtaaactc ataaaatcca caatccgtcg tgcgttctgt ttgtccatct
     5401 tgcgtaacga tttgagcgcc cgatcggaat agttaatcgt ccagaccaag tgacttcctc
     5461 acgtcttcag acgaatgcac tgcttcatca ccacggcgaa cgcgtgcagc agtttctgct
     5521 gaaagataat aatcctccag gtcttccaga tgcgccagta ttgcttcccg cgcataaaac
     5581 gtctttgttc tgccgg
//
