LOCUS       NZ_CP101771             5596 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Klebsiella pneumoniae strain hvKP323 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP101771
VERSION     NZ_CP101771.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29889746
            Assembly: GCF_024496125.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,W., Luo,Y., Zi,C., Yang,S., Zhang,J. and Zhao,Z.
  TITLE     Hypervirulent Klebsiella pneumoniae 323complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,W., Luo,Y., Zi,C., Yang,S., Zhang,J. and Zhao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2022) Department of Microbiology and Immunology of
            Basical Medicine, Guangdong Medical University, No. 1, Xincheng
            Avenue, Songshan Lake, Dongguan City, Guangdong, Dongguan, Guangdong
            523808, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUN-2022
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:23:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,889
            CDSs (total)                      :: 5,768
            Genes (coding)                    :: 5,583
            CDSs (with protein)               :: 5,583
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 63 of 185
            Pseudo Genes (incomplete)         :: 128 of 185
            Pseudo Genes (internal stop)      :: 23 of 185
            Pseudo Genes (multiple problems)  :: 29 of 185
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101771.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="hvKP323"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="China:Zhanjiang"
                     /collection_date="2022-04-16T16:24:40Z"
     gene            complement(join(5489..5596,1..402))
                     /locus_tag="NO368_RS26940"
                     /old_locus_tag="NO368_26940"
     CDS             complement(join(5489..5596,1..402))
                     /locus_tag="NO368_RS26940"
                     /old_locus_tag="NO368_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<437..871)
                     /locus_tag="NO368_RS26945"
                     /old_locus_tag="NO368_26945"
                     /pseudo=""
     CDS             complement(<437..871)
                     /locus_tag="NO368_RS26945"
                     /old_locus_tag="NO368_26945"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1080..1424)
                     /locus_tag="NO368_RS26950"
                     /old_locus_tag="NO368_26950"
     CDS             complement(1080..1424)
                     /locus_tag="NO368_RS26950"
                     /old_locus_tag="NO368_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1414..1590)
                     /locus_tag="NO368_RS26955"
                     /old_locus_tag="NO368_26955"
     CDS             complement(1414..1590)
                     /locus_tag="NO368_RS26955"
                     /old_locus_tag="NO368_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2746..3072
                     /locus_tag="NO368_RS26960"
                     /old_locus_tag="NO368_26960"
     CDS             2746..3072
                     /locus_tag="NO368_RS26960"
                     /old_locus_tag="NO368_26960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            3098..3643
                     /locus_tag="NO368_RS26965"
                     /old_locus_tag="NO368_26965"
     CDS             3098..3643
                     /locus_tag="NO368_RS26965"
                     /old_locus_tag="NO368_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3709..4059
                     /locus_tag="NO368_RS26970"
                     /old_locus_tag="NO368_26970"
     CDS             3709..4059
                     /locus_tag="NO368_RS26970"
                     /old_locus_tag="NO368_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4129..4395)
                     /locus_tag="NO368_RS26975"
                     /old_locus_tag="NO368_26975"
     CDS             complement(4129..4395)
                     /locus_tag="NO368_RS26975"
                     /old_locus_tag="NO368_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4379..4603)
                     /locus_tag="NO368_RS26980"
                     /old_locus_tag="NO368_26980"
     CDS             complement(4379..4603)
                     /locus_tag="NO368_RS26980"
                     /old_locus_tag="NO368_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(5270..5482)
                     /locus_tag="NO368_RS26985"
                     /old_locus_tag="NO368_26985"
     CDS             complement(5270..5482)
                     /locus_tag="NO368_RS26985"
                     /old_locus_tag="NO368_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1677..1753)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1942..2339
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3..1090)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.725"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5155..5594)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.7199999999999997e-67"
ORIGIN
        1 gttgctgtct tccaggtacg tcacgcccca ggtcttcgcg ttcagcttcg acagcgctgc
       61 gttctgccgg tcgatttcgg ccagatttga cgctatcaag ctcccctgat accacaacac
      121 cccgctcagg ctggcgaaca gcagtcctga caccatcgtg atggtcagcc atgtccgccc
      181 gaccatacgc atcacgctca ggcggttcga ctgcatggcc gcgctcatac cctgctcgtg
      241 ggcgcggatg gcggcgctga ttctcttccc gctttcgctc agttccgctc tgacagactt
      301 ctcgtgctcg ctgaatgcgg ctttcagcat ttcgccggta ctctgctgct gcgctttcga
      361 tttctgctct aagtccttcg ccagcgttaa aagactgttc atagatggct cccttaagtc
      421 ggatgtttcg ccctccgtcc gggtcggcaa tgctgatgct gctctttgtg gtgcgcacga
      481 cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg acgcgtttta agctcccctg
      541 acgaggcgag ggccagtaag ccccgcgtaa tagcctctgc ggcttcctgc ttcgttttgg
      601 gtaacgaaga tggcgtcacc agcgcgcgcc ggttctccgg tgcgttcggg tcgtgcagcc
      661 ccagtcgccc gttcaccacg gtctgccacg catcgatgcg cggacggtct gcccggtcgt
      721 agtacggctg gagacgcctg ccggtcagca gttccgtgtt cggtaccaga aaattcagtt
      781 ccagccgccc cttgtcccgg tgttcaaccc acagcacgct gtactggtct ttatcgagtc
      841 cgggcatcag aacccgctcg aagctcgcca tcagcttttc acgctggccg ggcggtaaat
      901 cctgttcagc aaaagacagg acgccggagg tgtacttttt ggcgtagggc gaggcgtcga
      961 taagctcccg gacctcctcc ggctttccct gcaggacgat ggccccgtcg cgctggcggt
     1021 ctttccccag cagataatcc accggcccgg caccgccgcc tcgccctcgc ggatgaaact
     1081 taacgatcat cgtctgcccc cttttccagc acggcatgcc gcagccgctc gagttcggca
     1141 tcaatggcca tcagcgcggc caccacctga acccggtcag cgcctgacca ctggccgccg
     1201 ttaattttcc gggcgatctg gttgaggttg ttccccatcc cggcaagctg gcgcagcaaa
     1261 accggatcga tggacggcag ccgcaacgac cgcgccgggc gcgtatcgag acaggtttcc
     1321 cgcatccaca ccgccagttg ccttccattg cagcgctcca gcagctgctg gtactcccca
     1381 tcggtcaccc ggatacctaa aaaacgggta cgcttaaccg ccatcctctc ctccgatatc
     1441 cgcaaaatgt gaccgggctg aacgctcaag ctctgccatc tgctcccggc ggtaatcttc
     1501 ctcctgtgcc tgacgcatca gatttgccat cactgccgga tgccaggatc ttttttttac
     1561 gacgagcggc ttcttcttcg gcttctgcat tatccctcct tcgcaaacgc cggacggcac
     1621 ggaaaagttt tccgggtcgc tccggcgttg gacaagcgcc agcgcgtcag tggtgttagc
     1681 gggtttcggg gcgcagccct gaaccagtca cagcgtttac gctgtgtata ctggcttact
     1741 atgttggcat gagagcacga ttcatgaaag tgcctcatgt ggcaggtgtg atatgtggct
     1801 caggagcgtc cgcaatacca tgcacggaca gatgaaatga cgcctcctcg ctcactcggt
     1861 agctgcgctc ctgccgttcg gctgcggcgg gcgtgaccga ctcacggata acgggataaa
     1921 gcaaaggcgc tcaaatcaga aagggccgga gaaaaggatg cggcgttgcc gtttttccat
     1981 aggctccgcc tcccctgacg agcatcacag aaatggacgc tcaagtcaga ggaggacgaa
     2041 acccgacagg acttaaagat gccggtgatt tctctccggc ggctccctcg tgcgctctcc
     2101 tgttccgacc ctgccgttta ccggatacct gttccgcctt tttccctttc gggaagcgtg
     2161 gcgctttctc atagctcacg ctgctggtat ctcggctcgg tttaggtcgt tcgctccaag
     2221 ctgggctgta tgcacgaacc ccccgttcag cccgactgct gcgccctttc cggtaactat
     2281 catcttgagt ccaacccgga cggacacgca aaaacgccac tggcagcagc cattggtaac
     2341 tggatatata cgagagagat attgagaggt cttgaagtgg gtgcctaact gcggctacac
     2401 tgaaagaaca gttttggtat ctgcggtctc gtatacccag ttacccggtt aagcagttcc
     2461 ccaactgact taaccttcga tcaaaccgcc tccccaggcg gttttttcgt ttacagtgca
     2521 agagattacg cgcagaaaaa aaggatctca agaagatcct ttaaatgatc gtcagttctg
     2581 ttatccccgg agctccatcc atcccacgga aaaacacccg ccactgatgc cctcagcgca
     2641 ctgcacatca tgtacaaacc actccctccc tcctttttta ttgcgtttct taatgcgcaa
     2701 tggtagcttt gtgcgcaaca aaaaacgcaa tagcaagggg gggtaatgag agaaagaaca
     2761 aatatcatgc tagatatagg attaaagcaa tccttacaac ggctttcgaa taaaaccaaa
     2821 atgagtctaa gtgatcttat taaccagaga ttatcaggtt cactttcagt tgaagaacgc
     2881 attggcgaaa agcctgaatg gatgagaaac cttgatccct atctcgccaa tcacttttca
     2941 aatattgagt taccgctccc ttcaccatta aaagaatacc aagaaaaaat ggatgagata
     3001 gattcaatta ttaatgacat gatggttcta aaggaatatt tcagagctaa gttttacgac
     3061 gaaataaaat aggcacgcta tacggaggcg gagaattatg ttttttaaac ggtatttaac
     3121 ggagaaggaa atatacaagg cagcactccc aattgttagg cttcaaggga gagttaacac
     3181 ttcattacgt tttttatcca aatactcaga tgtaaacaaa aatggattaa ttgcattgca
     3241 tcttaattta ttacatttta tctcatacaa catacacggt gtagagtctc tgaaaatcac
     3301 tcaagcaggc ataaacaaag ctgtacaaca aagccctcac gcagatgaaa taaaaaatat
     3361 tgcgcaggca ataggacgca aaggacctga gtacatcgaa ggattaatta gaaacccatg
     3421 ggggaagaaa aatgataatc cactaatatt tataatgagc attgaagaaa gtataaaaga
     3481 aacccttaac tcaggttata tgttatatag agtagctccc ataactgagg gtgaagcagc
     3541 tgctttcatg gatgttctta aacaattatt attatttgag gataattttc gcctttttgg
     3601 tagagacttg gtttataaaa tagaaaaatt aaaaatcaga taacaaagta tggtgaacga
     3661 aaaatgaaag agaggatagt gaaatgtgta ataccaagat tctcaactat gtattggtta
     3721 attgcgttcc tagcatttat attccccggt cgatcttttg ctgacggtta caccgcttac
     3781 taccgatgta atggtactca ggaagcatct cttactgtta aagataacaa actaagtatg
     3841 gttgtaggtc tttctaagtt taataggcaa ctggatactt atagattcaa agaaaaggga
     3901 atcgaagtag taatgcagag agctgcttct gatgataact ctgaggccat aatgtctttt
     3961 attatgcctg acgttaacaa tatgacctta ttagttaaaa ataacggcta cattatcgtt
     4021 aagacaacct gctggttaac taaaaccgac aaacagtaga acacgtagtt agtcgagtat
     4081 ttaccataat acctgttgtc ggcatcaaaa aagcccgcat agcgggcttc agtcgtaaac
     4141 ttcgcggcga tgtccaatcg tggcggcaag gataacaagc tcgtcatctc ggatctcaca
     4201 cagaacgcga taatctccca cccgatagcg ccagaactcg cccagctcac ctttgagcgg
     4261 cttccctgac tggcgaggat cggcagcaac tgcaatgcgt aaactcataa aatccacaat
     4321 ccgtcgtgcg ttctgtttgt ccatcttgcg taacgatttg agcgcccgat cggaatagtt
     4381 aatcgtccag accaagtgac ttcctcacgt cttcagacga atgcactgct tcatcaccac
     4441 ggcgaacgcg tgcagcagtt tctgctgaaa gataataatc ctccaggtct tccagatgcg
     4501 ccagtattgc ttcccgcgca taaaacgtct ttgttctgcc ggtttgcttc gccagcgagt
     4561 ccagacggga ctcaatctca tcagataacc ggatagcaag catataaacc tcccgaacac
     4621 catttgctat acatgtatat caacagtacc acaatcaacc taaaagaaca aaaaataatc
     4681 ttgttagccc ctcttgataa tccgtaacgc ggtcaatata tctagcttga gggctttcct
     4741 cttaactatt gaaaggataa cgattatggg tcttaagccg ggtccgaaac gaattgccga
     4801 ttcaacaggg gaacctgata aacgccagcg tgacaataaa aaaacgccgg gaaatactga
     4861 caaattgaaa cccagcaaat cttcaaaaaa ataagtcagt agttaaaagc cctccggggc
     4921 ttttttgtat ctgccgttca cgctgattaa acataacacc cgttgcccgc accgccccgg
     4981 acgggttcgc cgcgaacggg gtggcagtca ggtaaaaccg gcaacaaacc acccgataaa
     5041 ccgccccgtt cgcgacagat aaattttacc tggctgcata gccatgcagc caggtgaatg
     5101 acatccggtt tcagcgtaaa tgcccgatga gaaccctgtt ttcctgcatt tcagcccagc
     5161 gacggaccgt catagcttcg ctgccgggaa cgcgcttccg tcagttcctt ttcgtgctgc
     5221 cgttgctcgt gcgcccgttc tgcggccacg gccatctcat gacgctgcac taccggctca
     5281 aacgctgcag ttgcccgctc aagcgttcca cctgctgact caagcgcgtc acgcgctcgc
     5341 tcagcgccgc gttctcccgt tgcataagcc ctgacatttt ccgccattcc ccgaaggcgc
     5401 tctcccactc gtccagcctt ttcgagtagt cctgctgtag ctgctctaat gcgctcagca
     5461 actgtttttc cagctcggtc atcggcactt actccttcac cagtctcagc gcgttctgct
     5521 tcgaccgccc gttcccgacc gtccagctct gcgtcctgtc tacggccgtc cccttcggca
     5581 gcaccagaaa acgtcc
//
