LOCUS       NZ_CP101769             5596 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Klebsiella pneumoniae strain hvKP319 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP101769
VERSION     NZ_CP101769.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29889745
            Assembly: GCF_024496145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Wang,W., Luo,Y., Zi,C., Yang,S., Zhang,J. and Zhao,Z.
  TITLE     Hypervirulent Klebsiella pneumoniae 319 complete genome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Wang,W., Luo,Y., Zi,C., Yang,S., Zhang,J. and Zhao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2022) Department of Microbiology and Immunology of
            Basical Medicine, Guangdong Medical University, No. 1, Xincheng
            Avenue, Songshan Lake, Dongguan City, Guangdong, Dongguan, Guangdong
            523808, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 15-JUN-2022
            Assembly Method                   :: ABySS v. 2.0.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:28:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,009
            CDSs (total)                      :: 5,888
            Genes (coding)                    :: 5,679
            CDSs (with protein)               :: 5,679
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 73 of 209
            Pseudo Genes (incomplete)         :: 145 of 209
            Pseudo Genes (internal stop)      :: 26 of 209
            Pseudo Genes (multiple problems)  :: 35 of 209
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101769.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="hvKP319"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Zhanjiang"
                     /collection_date="2022-04-16T16:22:30Z"
     gene            742..1068
                     /locus_tag="NPJ83_RS30005"
                     /old_locus_tag="NPJ83_30005"
     CDS             742..1068
                     /locus_tag="NPJ83_RS30005"
                     /old_locus_tag="NPJ83_30005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1094..1639
                     /locus_tag="NPJ83_RS30010"
                     /old_locus_tag="NPJ83_30010"
     CDS             1094..1639
                     /locus_tag="NPJ83_RS30010"
                     /old_locus_tag="NPJ83_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            1705..2055
                     /locus_tag="NPJ83_RS30015"
                     /old_locus_tag="NPJ83_30015"
     CDS             1705..2055
                     /locus_tag="NPJ83_RS30015"
                     /old_locus_tag="NPJ83_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2125..2391)
                     /locus_tag="NPJ83_RS30020"
                     /old_locus_tag="NPJ83_30020"
     CDS             complement(2125..2391)
                     /locus_tag="NPJ83_RS30020"
                     /old_locus_tag="NPJ83_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2375..2599)
                     /locus_tag="NPJ83_RS30025"
                     /old_locus_tag="NPJ83_30025"
     CDS             complement(2375..2599)
                     /locus_tag="NPJ83_RS30025"
                     /old_locus_tag="NPJ83_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3266..3478)
                     /locus_tag="NPJ83_RS30030"
                     /old_locus_tag="NPJ83_30030"
     CDS             complement(3266..3478)
                     /locus_tag="NPJ83_RS30030"
                     /old_locus_tag="NPJ83_30030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3485..3994)
                     /locus_tag="NPJ83_RS30035"
                     /old_locus_tag="NPJ83_30035"
     CDS             complement(3485..3994)
                     /locus_tag="NPJ83_RS30035"
                     /old_locus_tag="NPJ83_30035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4029..4463)
                     /locus_tag="NPJ83_RS30040"
                     /old_locus_tag="NPJ83_30040"
                     /pseudo=""
     CDS             complement(<4029..4463)
                     /locus_tag="NPJ83_RS30040"
                     /old_locus_tag="NPJ83_30040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4672..5016)
                     /locus_tag="NPJ83_RS30045"
                     /old_locus_tag="NPJ83_30045"
     CDS             complement(4672..5016)
                     /locus_tag="NPJ83_RS30045"
                     /old_locus_tag="NPJ83_30045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5006..5182)
                     /locus_tag="NPJ83_RS30050"
                     /old_locus_tag="NPJ83_30050"
     CDS             complement(5006..5182)
                     /locus_tag="NPJ83_RS30050"
                     /old_locus_tag="NPJ83_30050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(5269..5345)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          2..335
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.614"
                     /coverage="81.0"
                     /evalue="2.28e-115"
     Typing          complement(3151..4682)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcacagaaat ggacgctcaa gtcagaggag gacgaaaccc gacaggactt aaagatgccg
       61 gtgatttctc tccggcggct ccctcgtgcg ctctcctgtt ccgaccctgc cgtttaccgg
      121 atacctgttc cgcctttttc cctttcggga agcgtggcgc tttctcatag ctcacgctgc
      181 tggtatctcg gctcggttta ggtcgttcgc tccaagctgg gctgtatgca cgaacccccc
      241 gttcagcccg actgctgcgc cctttccggt aactatcatc ttgagtccaa cccggacgga
      301 cacgcaaaaa cgccactggc agcagccatt ggtaactgga tatatacgag agagatattg
      361 agaggtcttg aagtgggtgc ctaactgcgg ctacactgaa agaacagttt tggtatctgc
      421 ggtctcgtat acccagttac ccggttaagc agttccccaa ctgacttaac cttcgatcaa
      481 accgcctccc caggcggttt tttcgtttac agtgcaagag attacgcgca gaaaaaaagg
      541 atctcaagaa gatcctttaa atgatcgtca gttctgttat ccccggagct ccatccatcc
      601 cacggaaaaa cacccgccac tgatgccctc agcgcactgc acatcatgta caaaccactc
      661 cctccctcct tttttattgc gtttcttaat gcgcaatggt agctttgtgc gcaacaaaaa
      721 acgcaatagc aagggggggt aatgagagaa agaacaaata tcatgctaga tataggatta
      781 aagcaatcct tacaacggct ttcgaataaa accaaaatga gtctaagtga tcttattaac
      841 cagagattat caggttcact ttcagttgaa gaacgcattg gcgaaaagcc tgaatggatg
      901 agaaaccttg atccctatct cgccaatcac ttttcaaata ttgagttacc gctcccttca
      961 ccattaaaag aataccaaga aaaaatggat gagatagatt caattattaa tgacatgatg
     1021 gttctaaagg aatatttcag agctaagttt tacgacgaaa taaaataggc acgctatacg
     1081 gaggcggaga attatgtttt ttaaacggta tttaacggag aaggaaatat acaaggcagc
     1141 actcccaatt gttaggcttc aagggagagt taacacttca ttacgttttt tatccaaata
     1201 ctcagatgta aacaaaaatg gattaattgc attgcatctt aatttattac attttatctc
     1261 atacaacata cacggtgtag agtctctgaa aatcactcaa gcaggcataa acaaagctgt
     1321 acaacaaagc cctcacgcag atgaaataaa aaatattgcg caggcaatag gacgcaaagg
     1381 acctgagtac atcgaaggat taattagaaa cccatggggg aagaaaaatg ataatccact
     1441 aatatttata atgagcattg aagaaagtat aaaagaaacc cttaactcag gttatatgtt
     1501 atatagagta gctcccataa ctgagggtga agcagctgct ttcatggatg ttcttaaaca
     1561 attattatta tttgaggata attttcgcct ttttggtaga gacttggttt ataaaataga
     1621 aaaattaaaa atcagataac aaagtatggt gaacgaaaaa tgaaagagag gatagtgaaa
     1681 tgtgtaatac caagattctc aactatgtat tggttaattg cgttcctagc atttatattc
     1741 cccggtcgat cttttgctga cggttacacc gcttactacc gatgtaatgg tactcaggaa
     1801 gcatctctta ctgttaaaga taacaaacta agtatggttg taggtctttc taagtttaat
     1861 aggcaactgg atacttatag attcaaagaa aagggaatcg aagtagtaat gcagagagct
     1921 gcttctgatg ataactctga ggccataatg tcttttatta tgcctgacgt taacaatatg
     1981 accttattag ttaaaaataa cggctacatt atcgttaaga caacctgctg gttaactaaa
     2041 accgacaaac agtagaacac gtagttagtc gagtatttac cataatacct gttgtcggca
     2101 tcaaaaaagc ccgcatagcg ggcttcagtc gtaaacttcg cggcgatgtc caatcgtggc
     2161 ggcaaggata acaagctcgt catctcggat ctcacacaga acgcgataat ctcccacccg
     2221 atagcgccag aactcgccca gctcaccttt gagcggcttc cctgactggc gaggatcggc
     2281 agcaactgca atgcgtaaac tcataaaatc cacaatccgt cgtgcgttct gtttgtccat
     2341 cttgcgtaac gatttgagcg cccgatcgga atagttaatc gtccagacca agtgacttcc
     2401 tcacgtcttc agacgaatgc actgcttcat caccacggcg aacgcgtgca gcagtttctg
     2461 ctgaaagata ataatcctcc aggtcttcca gatgcgccag tattgcttcc cgcgcataaa
     2521 acgtctttgt tctgccggtt tgcttcgcca gcgagtccag acgggactca atctcatcag
     2581 ataaccggat agcaagcata taaacctccc gaacaccatt tgctatacat gtatatcaac
     2641 agtaccacaa tcaacctaaa agaacaaaaa ataatcttgt tagcccctct tgataatccg
     2701 taacgcggtc aatatatcta gcttgagggc tttcctctta actattgaaa ggataacgat
     2761 tatgggtctt aagccgggtc cgaaacgaat tgccgattca acaggggaac ctgataaacg
     2821 ccagcgtgac aataaaaaaa cgccgggaaa tactgacaaa ttgaaaccca gcaaatcttc
     2881 aaaaaaataa gtcagtagtt aaaagccctc cggggctttt ttgtatctgc cgttcacgct
     2941 gattaaacat aacacccgtt gcccgcaccg ccccggacgg gttcgccgcg aacggggtgg
     3001 cagtcaggta aaaccggcaa caaaccaccc gataaaccgc cccgttcgcg acagataaat
     3061 tttacctggc tgcatagcca tgcagccagg tgaatgacat ccggtttcag cgtaaatgcc
     3121 cgatgagaac cctgttttcc tgcatttcag cccagcgacg gaccgtcata gcttcgctgc
     3181 cgggaacgcg cttccgtcag ttccttttcg tgctgccgtt gctcgtgcgc ccgttctgcg
     3241 gccacggcca tctcatgacg ctgcactacc ggctcaaacg ctgcagttgc ccgctcaagc
     3301 gttccacctg ctgactcaag cgcgtcacgc gctcgctcag cgccgcgttc tcccgttgca
     3361 taagccctga cattttccgc cattccccga aggcgctctc ccactcgtcc agccttttcg
     3421 agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg tttttccagc tcggtcatcg
     3481 gcacttactc cttcaccagt ctcagcgcgt tctgcttcga ccgcccgttc ccgaccgtcc
     3541 agctctgcgt cctgtctacg gccgtcccct tcggcagcac cagaaaacgt ccgttgctgt
     3601 cttccaggta cgtcacgccc caggtcttcg cgttcagctt cgacagcgct gcgttctgcc
     3661 ggtcgatttc ggccagattt gacgctatca agctcccctg ataccacaac accccgctca
     3721 ggctggcgaa cagcagtcct gacaccatcg tgatggtcag ccatgtccgc ccgaccatac
     3781 gcatcacgct caggcggttc gactgcatgg ccgcgctcat accctgctcg tgggcgcgga
     3841 tggcggcgct gattctcttc ccgctttcgc tcagttccgc tctgacagac ttctcgtgct
     3901 cgctgaatgc ggctttcagc atttcgccgg tactctgctg ctgcgctttc gatttctgct
     3961 ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg ctcccttaag tcggatgttt
     4021 cgccctccgt ccgggtcggc aatgctgatg ctgctctttg tggtgcgcac gacctcaaaa
     4081 cctgcgcttt ccagcgcctc agtgacgtcc tgacgcgttt taagctcccc tgacgaggcg
     4141 agggccagta agccccgcgt aatagcctct gcggcttcct gcttcgtttt gggtaacgaa
     4201 gatggcgtca ccagcgcgcg ccggttctcc ggtgcgttcg ggtcgtgcag ccccagtcgc
     4261 ccgttcacca cggtctgcca cgcatcgatg cgcggacggt ctgcccggtc gtagtacggc
     4321 tggagacgcc tgccggtcag cagttccgtg ttcggtacca gaaaattcag ttccagccgc
     4381 cccttgtccc ggtgttcaac ccacagcacg ctgtactggt ctttatcgag tccgggcatc
     4441 agaacccgct cgaagctcgc catcagcttt tcacgctggc cgggcggtaa atcctgttca
     4501 gcaaaagaca ggacgccgga ggtgtacttt ttggcgtagg gcgaggcgtc gataagctcc
     4561 cggacctcct ccggctttcc ctgcaggacg atggccccgt cgcgctggcg gtctttcccc
     4621 agcagataat ccaccggccc ggcaccgccg cctcgccctc gcggatgaaa cttaacgatc
     4681 atcgtctgcc cccttttcca gcacggcatg ccgcagccgc tcgagttcgg catcaatggc
     4741 catcagcgcg gccaccacct gaacccggtc agcgcctgac cactggccgc cgttaatttt
     4801 ccgggcgatc tggttgaggt tgttccccat cccggcaagc tggcgcagca aaaccggatc
     4861 gatggacggc agccgcaacg accgcgccgg gcgcgtatcg agacaggttt cccgcatcca
     4921 caccgccagt tgccttccat tgcagcgctc cagcagctgc tggtactccc catcggtcac
     4981 ccggatacct aaaaaacggg tacgcttaac cgccatcctc tcctccgata tccgcaaaat
     5041 gtgaccgggc tgaacgctca agctctgcca tctgctcccg gcggtaatct tcctcctgtg
     5101 cctgacgcat cagatttgcc atcactgccg gatgccagga tctttttttt acgacgagcg
     5161 gcttcttctt cggcttctgc attatccctc cttcgcaaac gccggacggc acggaaaagt
     5221 tttccgggtc gctccggcgt tggacaagcg ccagcgcgtc agtggtgtta gcgggtttcg
     5281 gggcgcagcc ctgaaccagt cacagcgttt acgctgtgta tactggctta ctatgttggc
     5341 atgagagcac gattcatgaa agtgcctcat gtggcaggtg tgatatgtgg ctcaggagcg
     5401 tccgcaatac catgcacgga cagatgaaat gacgcctcct cgctcactcg gtagctgcgc
     5461 tcctgccgtt cggctgcggc gggcgtgacc gactcacgga taacgggata aagcaaaggc
     5521 gctcaaatca gaaagggccg gagaaaagga tgcggcgttg ccgtttttcc ataggctccg
     5581 cctcccctga cgagca
//
