LOCUS       NZ_CP101730             5596 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 150040X1B1 plasmid p2_150040X1B1,
            complete sequence.
ACCESSION   NZ_CP101730
VERSION     NZ_CP101730.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29894129
            Assembly: GCF_024496265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yin,X., Feng,Y. and Zong,Z.
  TITLE     The complete genome of phage-resistant Klebsiella pneumoniae strain
            150040X1B1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yin,X., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2022) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:19:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,657
            Genes (coding)                    :: 5,488
            CDSs (with protein)               :: 5,488
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 115 of 169
            Pseudo Genes (internal stop)      :: 19 of 169
            Pseudo Genes (multiple problems)  :: 26 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101730.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="150040X1B1"
                     /isolation_source="passage"
                     /db_xref="taxon:573"
                     /plasmid="p2_150040X1B1"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2021"
     gene            join(5576..5596,1..306)
                     /locus_tag="NPN21_RS28845"
                     /old_locus_tag="NPN21_28845"
     CDS             join(5576..5596,1..306)
                     /locus_tag="NPN21_RS28845"
                     /old_locus_tag="NPN21_28845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            332..877
                     /locus_tag="NPN21_RS28850"
                     /old_locus_tag="NPN21_28850"
     CDS             332..877
                     /locus_tag="NPN21_RS28850"
                     /old_locus_tag="NPN21_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            943..1293
                     /locus_tag="NPN21_RS28855"
                     /old_locus_tag="NPN21_28855"
     CDS             943..1293
                     /locus_tag="NPN21_RS28855"
                     /old_locus_tag="NPN21_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1363..1629)
                     /locus_tag="NPN21_RS28860"
                     /old_locus_tag="NPN21_28860"
     CDS             complement(1363..1629)
                     /locus_tag="NPN21_RS28860"
                     /old_locus_tag="NPN21_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1613..1837)
                     /locus_tag="NPN21_RS28865"
                     /old_locus_tag="NPN21_28865"
     CDS             complement(1613..1837)
                     /locus_tag="NPN21_RS28865"
                     /old_locus_tag="NPN21_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(2504..2716)
                     /locus_tag="NPN21_RS28870"
                     /old_locus_tag="NPN21_28870"
     CDS             complement(2504..2716)
                     /locus_tag="NPN21_RS28870"
                     /old_locus_tag="NPN21_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2723..3232)
                     /locus_tag="NPN21_RS28875"
                     /old_locus_tag="NPN21_28875"
     CDS             complement(2723..3232)
                     /locus_tag="NPN21_RS28875"
                     /old_locus_tag="NPN21_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3267..3701)
                     /locus_tag="NPN21_RS28880"
                     /old_locus_tag="NPN21_28880"
                     /pseudo=""
     CDS             complement(<3267..3701)
                     /locus_tag="NPN21_RS28880"
                     /old_locus_tag="NPN21_28880"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3910..4254)
                     /locus_tag="NPN21_RS28885"
                     /old_locus_tag="NPN21_28885"
     CDS             complement(3910..4254)
                     /locus_tag="NPN21_RS28885"
                     /old_locus_tag="NPN21_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4244..4420)
                     /locus_tag="NPN21_RS28890"
                     /old_locus_tag="NPN21_28890"
     CDS             complement(4244..4420)
                     /locus_tag="NPN21_RS28890"
                     /old_locus_tag="NPN21_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(4507..4583)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4772..5169
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2389..3920)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgctagata taggattaaa gcaatcctta caacggcttt cgaataaaac caaaatgagt
       61 ctaagtgatc ttattaacca gagattatca ggttcacttt cagttgaaga acgcattggc
      121 gaaaagcctg aatggatgag aaaccttgat ccctatctcg ccaatcactt ttcaaatatt
      181 gagttaccgc tcccttcacc attaaaagaa taccaagaaa aaatggatga gatagattca
      241 attattaatg acatgatggt tctaaaggaa tatttcagag ctaagtttta cgacgaaata
      301 aaataggcac gctatacgga ggcggagaat tatgtttttt aaacggtatt taacggagaa
      361 ggaaatatac aaggcagcac tcccaattgt taggcttcaa gggagagtta acacttcatt
      421 acgtttttta tccaaatact cagatgtaaa caaaaatgga ttaattgcat tgcatcttaa
      481 tttattacat tttatctcat acaacataca cggtgtagag tctctgaaaa tcactcaagc
      541 aggcataaac aaagctgtac aacaaagccc tcacgcagat gaaataaaaa atattgcgca
      601 ggcaatagga cgcaaaggac ctgagtacat cgaaggatta attagaaacc catgggggaa
      661 gaaaaatgat aatccactaa tatttataat gagcattgaa gaaagtataa aagaaaccct
      721 taactcaggt tatatgttat atagagtagc tcccataact gagggtgaag cagctgcttt
      781 catggatgtt cttaaacaat tattattatt tgaggataat tttcgccttt ttggtagaga
      841 cttggtttat aaaatagaaa aattaaaaat cagataacaa agtatggtga acgaaaaatg
      901 aaagagagga tagtgaaatg tgtaatacca agattctcaa ctatgtattg gttaattgcg
      961 ttcctagcat ttatattccc cggtcgatct tttgctgacg gttacaccgc ttactaccga
     1021 tgtaatggta ctcaggaagc atctcttact gttaaagata acaaactaag tatggttgta
     1081 ggtctttcta agtttaatag gcaactggat acttatagat tcaaagaaaa gggaatcgaa
     1141 gtagtaatgc agagagctgc ttctgatgat aactctgagg ccataatgtc ttttattatg
     1201 cctgacgtta acaatatgac cttattagtt aaaaataacg gctacattat cgttaagaca
     1261 acctgctggt taactaaaac cgacaaacag tagaacacgt agttagtcga gtatttacca
     1321 taatacctgt tgtcggcatc aaaaaagccc gcatagcggg cttcagtcgt aaacttcgcg
     1381 gcgatgtcca atcgtggcgg caaggataac aagctcgtca tctcggatct cacacagaac
     1441 gcgataatct cccacccgat agcgccagaa ctcgcccagc tcacctttga gcggcttccc
     1501 tgactggcga ggatcggcag caactgcaat gcgtaaactc ataaaatcca caatccgtcg
     1561 tgcgttctgt ttgtccatct tgcgtaacga tttgagcgcc cgatcggaat agttaatcgt
     1621 ccagaccaag tgacttcctc acgtcttcag acgaatgcac tgcttcatca ccacggcgaa
     1681 cgcgtgcagc agtttctgct gaaagataat aatcctccag gtcttccaga tgcgccagta
     1741 ttgcttcccg cgcataaaac gtctttgttc tgccggtttg cttcgccagc gagtccagac
     1801 gggactcaat ctcatcagat aaccggatag caagcatata aacctcccga acaccatttg
     1861 ctatacatgt atatcaacag taccacaatc aacctaaaag aacaaaaaat aatcttgtta
     1921 gcccctcttg ataatccgta acgcggtcaa tatatctagc ttgagggctt tcctcttaac
     1981 tattgaaagg ataacgatta tgggtcttaa gccgggtccg aaacgaattg ccgattcaac
     2041 aggggaacct gataaacgcc agcgtgacaa taaaaaaacg ccgggaaata ctgacaaatt
     2101 gaaacccagc aaatcttcaa aaaaataagt cagtagttaa aagccctccg gggctttttt
     2161 gtatctgccg ttcacgctga ttaaacataa cacccgttgc ccgcaccgcc ccggacgggt
     2221 tcgccgcgaa cggggtggca gtcaggtaaa accggcaaca aaccacccga taaaccgccc
     2281 cgttcgcgac agataaattt tacctggctg catagccatg cagccaggtg aatgacatcc
     2341 ggtttcagcg taaatgcccg atgagaaccc tgttttcctg catttcagcc cagcgacgga
     2401 ccgtcatagc ttcgctgccg ggaacgcgct tccgtcagtt ccttttcgtg ctgccgttgc
     2461 tcgtgcgccc gttctgcggc cacggccatc tcatgacgct gcactaccgg ctcaaacgct
     2521 gcagttgccc gctcaagcgt tccacctgct gactcaagcg cgtcacgcgc tcgctcagcg
     2581 ccgcgttctc ccgttgcata agccctgaca ttttccgcca ttccccgaag gcgctctccc
     2641 actcgtccag ccttttcgag tagtcctgct gtagctgctc taatgcgctc agcaactgtt
     2701 tttccagctc ggtcatcggc acttactcct tcaccagtct cagcgcgttc tgcttcgacc
     2761 gcccgttccc gaccgtccag ctctgcgtcc tgtctacggc cgtccccttc ggcagcacca
     2821 gaaaacgtcc gttgctgtct tccaggtacg tcacgcccca ggtcttcgcg ttcagcttcg
     2881 acagcgctgc gttctgccgg tcgatttcgg ccagatttga cgctatcaag ctcccctgat
     2941 accacaacac cccgctcagg ctggcgaaca gcagtcctga caccatcgtg atggtcagcc
     3001 atgtccgccc gaccatacgc atcacgctca ggcggttcga ctgcatggcc gcgctcatac
     3061 cctgctcgtg ggcgcggatg gcggcgctga ttctcttccc gctttcgctc agttccgctc
     3121 tgacagactt ctcgtgctcg ctgaatgcgg ctttcagcat ttcgccggta ctctgctgct
     3181 gcgctttcga tttctgctct aagtccttcg ccagcgttaa aagactgttc atagatggct
     3241 cccttaagtc ggatgtttcg ccctccgtcc gggtcggcaa tgctgatgct gctctttgtg
     3301 gtgcgcacga cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg acgcgtttta
     3361 agctcccctg acgaggcgag ggccagtaag ccccgcgtaa tagcctctgc ggcttcctgc
     3421 ttcgttttgg gtaacgaaga tggcgtcacc agcgcgcgcc ggttctccgg tgcgttcggg
     3481 tcgtgcagcc ccagtcgccc gttcaccacg gtctgccacg catcgatgcg cggacggtct
     3541 gcccggtcgt agtacggctg gagacgcctg ccggtcagca gttccgtgtt cggtaccaga
     3601 aaattcagtt ccagccgccc cttgtcccgg tgttcaaccc acagcacgct gtactggtct
     3661 ttatcgagtc cgggcatcag aacccgctcg aagctcgcca tcagcttttc acgctggccg
     3721 ggcggtaaat cctgttcagc aaaagacagg acgccggagg tgtacttttt ggcgtagggc
     3781 gaggcgtcga taagctcccg gacctcctcc ggctttccct gcaggacgat ggccccgtcg
     3841 cgctggcggt ctttccccag cagataatcc accggcccgg caccgccgcc tcgccctcgc
     3901 ggatgaaact taacgatcat cgtctgcccc cttttccagc acggcatgcc gcagccgctc
     3961 gagttcggca tcaatggcca tcagcgcggc caccacctga acccggtcag cgcctgacca
     4021 ctggccgccg ttaattttcc gggcgatctg gttgaggttg ttccccatcc cggcaagctg
     4081 gcgcagcaaa accggatcga tggacggcag ccgcaacgac cgcgccgggc gcgtatcgag
     4141 acaggtttcc cgcatccaca ccgccagttg ccttccattg cagcgctcca gcagctgctg
     4201 gtactcccca tcggtcaccc ggatacctaa aaaacgggta cgcttaaccg ccatcctctc
     4261 ctccgatatc cgcaaaatgt gaccgggctg aacgctcaag ctctgccatc tgctcccggc
     4321 ggtaatcttc ctcctgtgcc tgacgcatca gatttgccat cactgccgga tgccaggatc
     4381 ttttttttac gacgagcggc ttcttcttcg gcttctgcat tatccctcct tcgcaaacgc
     4441 cggacggcac ggaaaagttt tccgggtcgc tccggcgttg gacaagcgcc agcgcgtcag
     4501 tggtgttagc gggtttcggg gcgcagccct gaaccagtca cagcgtttac gctgtgtata
     4561 ctggcttact atgttggcat gagagcacga ttcatgaaag tgcctcatgt ggcaggtgtg
     4621 atatgtggct caggagcgtc cgcaatacca tgcacggaca gatgaaatga cgcctcctcg
     4681 ctcactcggt agctgcgctc ctgccgttcg gctgcggcgg gcgtgaccga ctcacggata
     4741 acgggataaa gcaaaggcgc tcaaatcaga aagggccgga gaaaaggatg cggcgttgcc
     4801 gtttttccat aggctccgcc tcccctgacg agcatcacag aaatggacgc tcaagtcaga
     4861 ggaggacgaa acccgacagg acttaaagat gccggtgatt tctctccggc ggctccctcg
     4921 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt tttccctttc
     4981 gggaagcgtg gcgctttctc atagctcacg ctgctggtat ctcggctcgg tttaggtcgt
     5041 tcgctccaag ctgggctgta tgcacgaacc ccccgttcag cccgactgct gcgccctttc
     5101 cggtaactat catcttgagt ccaacccgga cggacacgca aaaacgccac tggcagcagc
     5161 cattggtaac tggatatata cgagagagat attgagaggt cttgaagtgg gtgcctaact
     5221 gcggctacac tgaaagaaca gttttggtat ctgcggtctc gtatacccag ttacccggtt
     5281 aagcagttcc ccaactgact taaccttcga tcaaaccgcc tccccaggcg gttttttcgt
     5341 ttacagtgca agagattacg cgcagaaaaa aaggatctca agaagatcct ttaaatgatc
     5401 gtcagttctg ttatccccgg agctccatcc atcccacgga aaaacacccg ccactgatgc
     5461 cctcagcgca ctgcacatca tgtacaaacc actccctccc tcctttttta ttgcgtttct
     5521 taatgcgcaa tggtagcttt gtgcgcaaca aaaaacgcaa tagcaagggg gggtaatgag
     5581 agaaagaaca aatatc
//
