LOCUS       NZ_CP101729            10060 bp    DNA     circular CON 15-AUG-2023
DEFINITION  Klebsiella pneumoniae strain 150040X1B1 plasmid p1_150040X1B1,
            complete sequence.
ACCESSION   NZ_CP101729
VERSION     NZ_CP101729.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29894129
            Assembly: GCF_024496265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Yin,X., Feng,Y. and Zong,Z.
  TITLE     The complete genome of phage-resistant Klebsiella pneumoniae strain
            150040X1B1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Yin,X., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JUL-2022) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.15.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/15/2023 08:19:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,657
            Genes (coding)                    :: 5,488
            CDSs (with protein)               :: 5,488
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 64 of 169
            Pseudo Genes (incomplete)         :: 115 of 169
            Pseudo Genes (internal stop)      :: 19 of 169
            Pseudo Genes (multiple problems)  :: 26 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101729.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="150040X1B1"
                     /isolation_source="passage"
                     /db_xref="taxon:573"
                     /plasmid="p1_150040X1B1"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2021"
     gene            complement(join(9988..10060,1..299))
                     /gene="tolA"
                     /locus_tag="NPN21_RS28780"
                     /old_locus_tag="NPN21_28780"
     CDS             complement(join(9988..10060,1..299))
                     /gene="tolA"
                     /locus_tag="NPN21_RS28780"
                     /old_locus_tag="NPN21_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(573..818)
                     /locus_tag="NPN21_RS28785"
                     /old_locus_tag="NPN21_28785"
     CDS             complement(573..818)
                     /locus_tag="NPN21_RS28785"
                     /old_locus_tag="NPN21_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1145..2830
                     /locus_tag="NPN21_RS28790"
                     /old_locus_tag="NPN21_28790"
     CDS             1145..2830
                     /locus_tag="NPN21_RS28790"
                     /old_locus_tag="NPN21_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2840..3097
                     /locus_tag="NPN21_RS28795"
                     /old_locus_tag="NPN21_28795"
     CDS             2840..3097
                     /locus_tag="NPN21_RS28795"
                     /old_locus_tag="NPN21_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            3182..3331
                     /locus_tag="NPN21_RS28800"
                     /old_locus_tag="NPN21_28800"
     CDS             3182..3331
                     /locus_tag="NPN21_RS28800"
                     /old_locus_tag="NPN21_28800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            4815..6779
                     /locus_tag="NPN21_RS28805"
                     /old_locus_tag="NPN21_28805"
     CDS             4815..6779
                     /locus_tag="NPN21_RS28805"
                     /old_locus_tag="NPN21_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6779..7510
                     /gene="mobC"
                     /locus_tag="NPN21_RS28810"
                     /old_locus_tag="NPN21_28810"
     CDS             6779..7510
                     /gene="mobC"
                     /locus_tag="NPN21_RS28810"
                     /old_locus_tag="NPN21_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            7517..8047
                     /locus_tag="NPN21_RS28815"
                     /old_locus_tag="NPN21_28815"
     CDS             7517..8047
                     /locus_tag="NPN21_RS28815"
                     /old_locus_tag="NPN21_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8074..8253)
                     /locus_tag="NPN21_RS28820"
                     /old_locus_tag="NPN21_28820"
     CDS             complement(8074..8253)
                     /locus_tag="NPN21_RS28820"
                     /old_locus_tag="NPN21_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8279..8707)
                     /locus_tag="NPN21_RS28825"
                     /old_locus_tag="NPN21_28825"
     CDS             complement(8279..8707)
                     /locus_tag="NPN21_RS28825"
                     /old_locus_tag="NPN21_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(8871..9185)
                     /locus_tag="NPN21_RS28830"
                     /old_locus_tag="NPN21_28830"
     CDS             complement(8871..9185)
                     /locus_tag="NPN21_RS28830"
                     /old_locus_tag="NPN21_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9173..9451)
                     /locus_tag="NPN21_RS28835"
                     /old_locus_tag="NPN21_28835"
     CDS             complement(9173..9451)
                     /locus_tag="NPN21_RS28835"
                     /old_locus_tag="NPN21_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(9626..9991)
                     /locus_tag="NPN21_RS28840"
                     /old_locus_tag="NPN21_28840"
     CDS             complement(9626..9991)
                     /locus_tag="NPN21_RS28840"
                     /old_locus_tag="NPN21_28840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1..7830
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3435..4013)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6780..7507
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gcgtgggtaa cggctgatat tgctgcttta cagagtttcg gatctcctcc ttcagcaatg
       61 gcgctgttca gagtgccatc acgttgtatg gatatccgca cggtgcacat ttttcccgca
      121 taggcctctg gttcaaacat ctgcatttca acagcggtct tgatggccgc agcgtagcta
      181 ctgattttag atgacttatc cagagtataa attttcggcg gctgggtggt ggaatgctta
      241 caggccgtca ggagtaaggg gctgcacaat gccagcagga taaagggtat taaacgcatc
      301 gggttgatga cctgtcagta aaaaatgtta catgtatatt tgtcactctt tacggccacg
      361 ttcatttaca taaacacggc ttatcgtatc gtttttacac caaacggccc gccatgcagt
      421 tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg cgtttccggc ggttttccgg
      481 gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg ccctcagacc gtctgagcgg
      541 tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc ggcgctgtgg ctgccaggtg
      601 aaggtggttt ccgtagcctc gtatttctcc cgacgcattt cctgcgcccg cctggcttca
      661 cgttcgtcct gaatggcacg aagcaccctt tcccgcacct gttgttttac cgcctttggc
      721 agcgttttgt tccctttcag gcccatggcc agtgcctgag cgacaaaggc gtccgccagg
      781 cgcttttctt cagcgggagt cagtttcagg tgcgtcatag tggcctccgg gcgtctcagg
      841 tttcccgaac agtttaaccg caggtcctct cctcatgcca gttagctttc agatgtgtct
      901 ataccatctg aaatgtaagt gtgatgaatg tcttaaataa taaaatttgt tgccatgaga
      961 aattttcggg aaaagacgcg atctgcttac aggtggctcc gggcgctttt ttgtgcctga
     1021 agatgagaac taatctcata tattgacacc tgaaaacggg aggagtaagg taataattat
     1081 actgtgtata tgtacagtat gttggcttta atttattgtt ttaaatgtca aagaggaaaa
     1141 cggtatgagt ggtggagacg gtcgaggtcc gggtaattca ggtctgggac ataatggtgg
     1201 tcaggccagt gggaatgtga acggtacgtc tggtaaaggt ggcccttcat caggtggtgg
     1261 tacggatcca aacagcgggc cgggctgggg tacgacgcat acgccgaatg gagatattca
     1321 taactataat ccgggggagt ttggtaacgg cgggagtaaa cccggtggta atggtggtaa
     1381 cagcggcaat catagcggta gctctggtgg cggacagtct tcggccactg cgatggcctt
     1441 cggtctgcct gccctggcta ctccgggggc tgaaggactg gctttatcag tttccggcga
     1501 tgcgttgtcg gccgccgttg ctgatgtgct ggctgccctg aaggggccgt ttaagtttgg
     1561 tctgtggggg attgcgatct acggtgtgct gccttctgag attgcaaaag atgatccgaa
     1621 aatgatgtca aaaattatga cgtcattacc ggccgatgcg gtgacggaga ctccggcaag
     1681 tactttacca ctggaccagg cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa
     1741 ggatgagcgg cagcatattg cggttgtcgc aggtcggcca atgagtgtcc ctgtggtgga
     1801 tgcgaaaccg acaaaacgtc cgggggtatt cagtgtgtcg attccgggtc tcccgtctct
     1861 gcaggtgagc gtaccgaaag gtgttccgac agcgaaagcc ccgccaaaag gcattgttgc
     1921 tgaaaaaggt gattcacgtc cggctggttt tacagccggt ggtaactccc gtgaggccgt
     1981 tattcgtttc ccgaaagaga ccggacagaa gccggtttat gtgtcggtga cagatgttct
     2041 taccccggca caggtaaaac agcgtcagga ggaagaaaag cgtcgccagc aggcatggga
     2101 cgccgctcat ccggaagagg ggctgaaaag agactatgat aaagcgaaag ctgagctgga
     2161 tgccgaagat aaaaatattg cgaccttaaa cagccgcatt gcatcgacag agaaggcgct
     2221 ccccggtgca agggctgctg ttcaggaagc cgataaaaag gtaaaagagg cagaggcaaa
     2281 taaagatgat tttgtgactt ataacccgcc tcatgaatat ggctccgggt ggcaggatca
     2341 ggttcgctat cttgataagg atattcagaa tcagaatgag aaattaaaag cggctcaggc
     2401 atctttaaac gcaatgaatg aatccttatc cagagataag gctgcactta ccggggcgat
     2461 ggagagccgg aaacaaaagg agaaaaaagc gaaggatgca gaaaataagt taaatgagga
     2521 aaagaacaag cctcgcaagg ggactaaaga ctacggccat gattatcatc cagtacctaa
     2581 aacagaagac ataaagggac ttggagagtt aaagcgaggt gatcctaaaa cacctaaaca
     2641 aggtggtggt ggtaagcggg cccgatggta tggcgataaa aaacgtaaaa tttatgaatg
     2701 ggattcccag cacggtgagc ttgaagggta tcgtgccagt gatggcgaac acctaggcgc
     2761 atttgatccc aaaacgggta agcaggttaa agggccagat ccaaaacgga acattaaaaa
     2821 atatctttga gaggtaaata tggggcttaa attacgtttg acatggtttg ataagaaaac
     2881 tgaagagttt aaaggggaag agtattctaa agactttggt gacgatggtt ctgtcattga
     2941 aagcttggga atgcctttaa aggataatat taataatggt gagtttgatt tgaagaaaga
     3001 atgggttcct ttactacagc cattttttaa aaatcaaata aaacctgatg tggataattg
     3061 ttatatagct tttgattatc gggacggtaa ctggtaacaa aaaattatca aggatgattt
     3121 gaaatactga cttccggtgt tcagggatga atgccggagt ctttaaatac agggaaagta
     3181 tatgaaaaag gtaaagacta tttttctttt tatattgatt gttgctggct ttcttctggc
     3241 cgcatgtcag gcaaattata tccgggatgt tcagggcgga acggtggcac catcgtcctc
     3301 ctctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc ttgagatcct
     3361 ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg aggtggtttg
     3421 atcgaaggtt aagtcagttg gggaactgct taaccgtggt aactggcttt cgcagagcac
     3481 agcaaccaaa tctgtccttc cagtgtagcc ggactttggc gcacacttca agagcaaccg
     3541 cgtgtttagc taaacaaatc ctctgcgaac tcccagttac caatggctgc tgccagtggc
     3601 gttttgcgtg ctttcccggg ttggactcaa gtgaacagtt accggataag gcgcagcagt
     3661 cgggctgaac ggggagttct tgcttacagc ccagcttgga gcgaacgacc tacaccgagc
     3721 cgagatacca gtgtgtgagc tatgagaaag cgccacactt cccgcaaggg agaacggcgg
     3781 acaggtatcc ggtaaacggc agggtcggaa caggagagcg caagagggag cgacctaccg
     3841 gaaacggtgg ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga ttcatggttg
     3901 agcctcacgg ctcccacaga tgcaccgaaa aagcgtctgt ttatgtgaac tctggcagga
     3961 gggcggagcc tatggaaaaa cgccaccggc gcggccctgc cgttttgcct cacatgttag
     4021 tcccctgctt atccacggaa tctgtgggta actttgtatg tgtccgcagc gcccgccgca
     4081 gtctcacgcc cggagcgtag cgaccgagtg agcgaggagg cgcaatttcc ccggcacctg
     4141 caagacggtc accggacgcg ctgatgtggg atattgtccc ctgattaagt ctttctattc
     4201 cctgacgatc aattatcagg ataggtgaac catgtcggtt tttcgctgaa cattttacgt
     4261 taacctatct ctgttgcttt tttctccttg cggagaaatg caagtcatcc ccgctggggc
     4321 gtggctgact tcaggggaaa agaccttgct tacgctggct ggacgccacc gacgcacggt
     4381 cttttcccct ctttctggct ctgtccggct ggacgccggc cacgccagaa attcacccgg
     4441 cccccgtcag gctcacgcct gaccacccac aaaaatccac ggtgctggac gcacctgacg
     4501 aagcagcaaa gctgctcctg attttgcggg tggtcagccg ctcgctatgg cgaactctga
     4561 cgggggccgg aacaccggaa aagaccgcac tggggtgccg ccttttcctc tcacacgctg
     4621 gggcgtgctc gttgttcctc cggggctgcg ccttttccgc atggggcgga aagaggctgg
     4681 cctccgcggt gaaagctgaa cgcaatcacc acggacgtga ccgccgccgc catgccttgg
     4741 ggcatgtccg cggcttgtgc agcgggggct gcgcatcacg ccggtaaacc cgtcgtctga
     4801 aggagagaga ttgcatgttt aatacggatt cgcttgcctg gcagtggcat atcccggagc
     4861 tggcccggca gatttacccg tgggtcagct ggctgtaccg ggagtattac agtctggccg
     4921 gtttttttct ggttatcggc ctgtttttcg gtctggctga actctgggcc ggccgaatgc
     4981 gttttgtggg gctgagccat aaggcgcggc gggagtatgc cgatcgctat aagcgcgata
     5041 ataaccggac tgtgccattt tcggtgctgg cctcgctgga ttcacccggg cggatggtca
     5101 gggcacggcg acatgtctgg aactacgcgt actggctgac cggcgctgcc ggtctggccc
     5161 ttctgggggg gctggttgtc gccgtgttta tcgagagctg gggagaaacc gtcctgcgcc
     5221 cggacctcta tgcgaacgca gattattacc tgctgcagcc ggctaacctg ttcgggatag
     5281 cctggtgtgc cggagggctt gtcgccggcg tccttctggc gcggctgctg gcgttcctgc
     5341 tgcttgatgg tcattttgcg gccgctgacg aggcggtgaa cgcgaaactg aatcaggaga
     5401 gccggcgcag cagccagcgg accggagaaa tgacagacgt ccgccacctg catttcgggg
     5461 agccggtacc ggttaacgcg ctggccgatt tttccacgga gcaggcccgg aaacagcagg
     5521 cggtgtttct gggtaaggat gagcagggcc agccggtgct ggtccctcgt gatacctggc
     5581 gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg caagagcgcg atggccacca
     5641 acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt ggtgtacttc gaccccaaag
     5701 gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc ggacttcacc ctgctggatc
     5761 tgcggccggg gaaaccggcg cagctgaatc tgttccgcga tctcgaccag tacgccctga
     5821 agaatctgct ggttgccggg tttaacctga gtgagaccgg cgacgtggcg gaccactacc
     5881 gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gttcccgcag ggggcaaaca
     5941 ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc cctgaaaaag gacgtgaagg
     6001 ggctgatcac caagctggag aacgtggccg acctgagcgt cctgcagacg gacagcggta
     6061 tcgatgtggc ggggataatc aacggcggcg gctgcctgta cgtcatcggc tcgatggacg
     6121 atgaggcggt gatccgggtg cagaagatgc tgtttgcccg ctgcgcgcaa atcatcattg
     6181 cgcgggatga gttccggagg tggccgcacg cgagcatcat gctcgatgag attaaatatc
     6241 tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg cagccgggac tgcaacctgc
     6301 tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg gcaggatctg cccgctgatt
     6361 ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttctatcgc gcggccagcc
     6421 aggagtccgc gcagtgggcg gcgggccaga ccggggagat ccgggtggac gtggagcgcc
     6481 gccgggccag ccgggaggcg gggaacgtgg agcacatcag cggcgacagc ttcatccaga
     6541 aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca cctgcctgac gggttcgcgg
     6601 tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag cccgctgcgg gttgagcgcc
     6661 gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac ggacccgctg gcggagtacc
     6721 tgcctccggc cgacggcggc ggaccaaaag cggatgatga ttttgcgggg ctttactgat
     6781 gacactggag cagtacaacg tcagccacgc gaagcggcag gcgcggaacg cggagaaaac
     6841 acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg
     6901 acggctgggg attgcggcgg tacagccggt ttaccgcttc ctggacagtc tggtggcgaa
     6961 agggctgctg gtccgggcga aatacccggt tgacggccgt caggtcagcg tgtgggggct
     7021 gacgccgcac ggggtggcgt tctcgttcga cgaggacgag ccgctgacgg acgttattcc
     7081 gtttcagccg tcaagggtgt cggcggcgca gctgccgcac cggctggccg tgcagtccct
     7141 gcgcctctcc atggagggcc ggggggccac cggctggcgg tacctgcacc gtatggccct
     7201 gaaagggatg aaagtgccgg acgcgctggc ggagctggac ggcagaacgg tggcatttga
     7261 ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa gtggtgtccg ggtatctgtt
     7321 caaccgccgg gcgaacggca tcgatgaaat ctggtacatc tgccctgacc ggccgacgct
     7381 tgtccgggta cagcgggcga ttctggcggt ggatgaaatc gtgaatccac agaccggcga
     7441 ggcccgcaaa acggtggagc tggaccggga gaggctgttc gcgtgcttta aatttatgac
     7501 aacggagtga gggattatgg cgctgtacat ggtatttatt gaggacgtgg aagacaaaga
     7561 cggcgagcgc tgcattatgc caaccagcga gcatctggaa gacatggtgg tgctcgcctg
     7621 gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa
     7681 atccatcggg atgtatgacg agcggctcaa cagcgacgaa aaagccacgt actttttcac
     7741 cggtgaaaat ccgcaatggg aacgggacaa acccacagga atgaagctgt gtgattacct
     7801 ctatcgaaag gtggagcggt tcgccatcga gaacggtgtg caggtggttg atgccgggaa
     7861 gtgctcaccg gagcagatta acgcgatgat tgaggtggtc agcgagcggc acggggaaag
     7921 cgcggtgaac gagaagctga aagcggagct gatgcgggaa tataacagcc gctattttaa
     7981 cgtgccggtg cctgaactgc cggaggacaa acccgcccgc cggtggtggc cgttcggcaa
     8041 aaaataacgc cagcaccggc atggccggtg ctgttattct tccccgagct tatcccgcat
     8101 cacctgccag agtgtttccg catcctcgtg aagccgttcg cacatgtcgg ccggggtatc
     8161 catgtccagc tcgttcagct tttccagcaa cagcagcgac tgatccttaa tgaatttagc
     8221 catattgagg gcagtttttt cctgcttcgt catgttctcc attcccttcg gtaagttctc
     8281 agctaaacca gccggaccag aagcgttttt tcttctgtgt cagttctgtt gtcaatctgt
     8341 ccacttcggc ctggagtttc cggcgctcat catcgtgctg gcggcgttct tcttcccggg
     8401 atgttttatc ttccagcatc agggtgacag cctgccggag atcggccagt tccttttgca
     8461 ttgccagaag ctgttcctgt gggaaatttt tttgtgtatt ttcacggtgt gaaatgccgg
     8521 gaatttgttt ctcagggata ttcacatttc cgtatacacg gatcagttct gacacatcca
     8581 cgacagggtt gtttttcccg tcacgtgaca cggtaacctt tccctgtttt acatgattgt
     8641 atagcgttct tctggtaatg cccgcggctt gtgctgcctg tgaaaggttg agcaatgttt
     8701 tcgccactgt atacccctga ttttatcggg tgtgaaaggt gtgtaatttc tcacggtaaa
     8761 tgtatcgctg aattttaccc gtttttccgt tcaggatgtg gttttctgac gcgctgcgct
     8821 tgtcaaaccg gcgacggcca cgcaatgaat tgcgcccccg tcatcggtta ttagaggctg
     8881 tctgcccccg gatacccgga ttctttctga tgcctgacgg tgacgatcac aatctggtcc
     8941 atttcccggt cgtggcggta gagcatcaca tagccactgt cgccaaaccc gatcaccagt
     9001 tccttatatt ccagcggcag aaacggcacc gggcggccaa tgtctggcaa tgttttcagt
     9061 tgctggatag cccggacgat cacctcacct ccttttctgg ctgccagccg atttttggtt
     9121 ttgagaaagt cctgaaggcg ttgtaaatcg cgctgtgcca gcgcggaaat cgttacctgt
     9181 ggcatggcgg agcatcctgc tcgttctcag ttccccaggt acttatccag gcttccgctt
     9241 cttccgcagt aaggtgcaga cccgtttcct gatagtgctg ccatgcggcc tgtccgtccc
     9301 gcaggtactg gctgcgcttc tcttcccggt cgatgtattc ggtgattgcc tccagcatta
     9361 gcgcgtgcgc tgagcggtgc cggtcatcgg ccagcgtttt cagccggtcc ttaagttctt
     9421 catcgagcct gatggatgtt gctgatgaca taaaagcccc tttgtagtca tatgtattac
     9481 acatgactac tttaatccgt ggcggcgcta tttgccagaa atacggcttt tgctgcccat
     9541 ttttcagctt cgcttccact aatcagggct gctacaggct cactcatcat ccaggactat
     9601 tgcagcctgc gggttaaagg acctgttact ggcggcaacc atcatcattg agcttttggg
     9661 tggtgactgg tcgcggatcc tgtaagccgt catttttcag actgaattca aaatttataa
     9721 cctgtcgcat tgccgggtgc cctgcgggat atagccagtg cctcattgcc gtcagcgttt
     9781 cccgaaaaaa aaggtgacag ccggtaagcg acacgttgtc cactcgtcca tcgtccagaa
     9841 tatcaaacgt ggcggtcacc tgaccttcga tgcgttttgc tgcggcctgt accgggtagg
     9901 ttggctgcag tgtggttacg ggttttagtg gtatcgcatg taccggagtg aggaacatgg
     9961 ccgccagcac tgtcagagta aaagctttca tggtctgaaa tccagagggg cgtttttgaa
    10021 tatttgccag gtattttcat ccggtgccgg cggaattttg
//
