LOCUS       NZ_CP101690            10047 bp    DNA     circular CON 12-AUG-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Mbandaka strain SMEH
            plasmid pSMEH1, complete sequence.
ACCESSION   NZ_CP101690
VERSION     NZ_CP101690.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29857753
            Assembly: GCF_024494525.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Mbandaka
  ORGANISM  Salmonella enterica subsp. enterica serovar Mbandaka
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Zhou,A.
  TITLE     A strain pertaining to Salmonella enterica subsp. enterica serovar
            Mbandaka isolated from a patient with pericardial effusion
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Zhou,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2022) Medical Laboratory, Shanghai East Hospital,
            Yuntai Road 1800, Shanghai, Shanghai 200123, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2022
            Assembly Method                   :: Unicycler v. 0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/12/2023 06:01:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,744
            CDSs (total)                      :: 4,626
            Genes (coding)                    :: 4,513
            CDSs (with protein)               :: 4,513
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 35 of 113
            Pseudo Genes (incomplete)         :: 82 of 113
            Pseudo Genes (internal stop)      :: 26 of 113
            Pseudo Genes (multiple problems)  :: 27 of 113
            Pseudo Genes (short protein)      :: 1 of 113
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101690.1.
            Bacteria and source DNA available from Shanghai East Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Salmonella enterica subsp. enterica serovar
                     Mbandaka"
                     /mol_type="genomic DNA"
                     /strain="SMEH"
                     /serovar="Mbandaka"
                     /host="Homo sapiens"
                     /sub_species="enterica"
                     /db_xref="taxon:192954"
                     /plasmid="pSMEH1"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2021-09"
     gene            complement(join(9699..10047,1..137))
                     /locus_tag="NOV91_RS23535"
                     /old_locus_tag="NOV91_23535"
     CDS             complement(join(9699..10047,1..137))
                     /locus_tag="NOV91_RS23535"
                     /old_locus_tag="NOV91_23535"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012482081.1"
                     /translation="MSSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELNESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSVLLIASSAGILWWQSQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRSCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            388..633
                     /locus_tag="NOV91_RS23540"
                     /old_locus_tag="NOV91_23540"
     CDS             388..633
                     /locus_tag="NOV91_RS23540"
                     /old_locus_tag="NOV91_23540"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_256382264.1"
                     /translation="MQPQPAVHDGLPGIDARTVSAVVIRLEPFAGQQLRVRDQEVQLQP
                     PLVLVLHPQHAVLVFIESGHQNTLEARHQFFTLTGR"
                     /name="hypothetical protein"
     gene            complement(815..1138)
                     /locus_tag="NOV91_RS23545"
                     /old_locus_tag="NOV91_23545"
     CDS             complement(815..1138)
                     /locus_tag="NOV91_RS23545"
                     /old_locus_tag="NOV91_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_015059464.1"
                     /translation="MLTIWVTEDEHRRLLDRCDGKQLAAWMRQTCLDEKPARAAKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1788..1946
                     /locus_tag="NOV91_RS23550"
                     /old_locus_tag="NOV91_23550"
     CDS             1788..1946
                     /locus_tag="NOV91_RS23550"
                     /old_locus_tag="NOV91_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001658417.1"
                     /translation="MHEPPVQSDCCASSGNFQLDSNPERHDKAPLAAAIGNWELVDLDI
                     ESLEVVA"
                     /name="hypothetical protein"
     gene            complement(2189..2494)
                     /locus_tag="NOV91_RS23555"
                     /old_locus_tag="NOV91_23555"
     CDS             complement(2189..2494)
                     /locus_tag="NOV91_RS23555"
                     /old_locus_tag="NOV91_23555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA];
                     GO:0046872 - metal ion binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="WP_223229636.1"
                     /translation="MPKPDNSCCRSEDIVCRFGGEEFVVFLPNTSVVTAECVAERIRSV
                     IESTVFPNNLRVTVSAGVATQEDPLGGIDFLLKNADDALYQAKGEGRNKVIVYSAI"
                     /name="GGDEF domain-containing protein"
     gene            complement(<2480..3510)
                     /locus_tag="NOV91_RS23560"
                     /old_locus_tag="NOV91_23560"
                     /pseudo=""
     CDS             complement(<2480..3510)
                     /locus_tag="NOV91_RS23560"
                     /old_locus_tag="NOV91_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            3905..4561
                     /locus_tag="NOV91_RS23565"
                     /old_locus_tag="NOV91_23565"
     CDS             3905..4561
                     /locus_tag="NOV91_RS23565"
                     /old_locus_tag="NOV91_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            complement(4880..5494)
                     /locus_tag="NOV91_RS23570"
                     /old_locus_tag="NOV91_23570"
     CDS             complement(4880..5494)
                     /locus_tag="NOV91_RS23570"
                     /old_locus_tag="NOV91_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_014839983.1"
                     /translation="MFIGYIRMSKADGSQTNDLQRDALIAAGVDAAHFYEDRASGKREE
                     RPGLTACLKALRPGDTLVVWKLDRLGRDLRHLINTVHDLTARGTGLKVLTGHGATIDTT
                     TAAGKLVFGIFAALAEFERELIAERTTAGLASARARGRNGGRPYKMTPVKLRLAMASMG
                     QSETKVSTLCQELGITRQTLYRHISPVGQLRADGIKLFNRG"
                     /name="recombinase family protein"
     gene            5656..>5838
                     /locus_tag="NOV91_RS23575"
                     /old_locus_tag="NOV91_23575"
                     /pseudo=""
     CDS             5656..>5838
                     /locus_tag="NOV91_RS23575"
                     /old_locus_tag="NOV91_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            5974..6519
                     /locus_tag="NOV91_RS23580"
                     /old_locus_tag="NOV91_23580"
     CDS             5974..6519
                     /locus_tag="NOV91_RS23580"
                     /old_locus_tag="NOV91_23580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_001493763.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGIS
                     YEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCIS
                     GHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRL
                     DLASPVLEPEVIWLGRRR"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            6656..7228
                     /locus_tag="NOV91_RS23585"
                     /old_locus_tag="NOV91_23585"
     CDS             6656..7228
                     /locus_tag="NOV91_RS23585"
                     /old_locus_tag="NOV91_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001493762.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNRP
                     GLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGTNN
                     PMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALRSRG
                     VRLRIACDHAGLGVSTYYKLRHQMNNQ"
                     /name="recombinase family protein"
     gene            7265..>7870
                     /locus_tag="NOV91_RS23590"
                     /old_locus_tag="NOV91_23590"
                     /pseudo=""
     CDS             7265..>7870
                     /locus_tag="NOV91_RS23590"
                     /old_locus_tag="NOV91_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /name="ISKra4-like element ISKpn19 family transposase"
     gene            complement(7798..9174)
                     /locus_tag="NOV91_RS23595"
                     /old_locus_tag="NOV91_23595"
     CDS             complement(7798..9174)
                     /locus_tag="NOV91_RS23595"
                     /old_locus_tag="NOV91_23595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900944.1"
                     /GO_function="GO:0009975 - cyclase activity [Evidence IEA]"
                     /GO_process="GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="WP_080196049.1"
                     /translation="MVNVEFFNYIECIFMRIKKPHLRPIVITLSVGGILLTSIFLIVVI
                     MVFKRENIEKDLLDANSSYAMKMSDVMSNYIEIAQRELAYGAKKIESTNDIDSLKNEAD
                     RLRLQSGMFNSVLVVSDGAVVLATSPESLGLVGVHLNSSASKLAIEAKKPFISAPYKSA
                     SGNLIIFLSHPIYDKNSNYIGYVGGSIYLKEQSLFSDVLSRHFFKSDTEISIVSDDGNV
                     IFNNDNSAVGRPMVMDDNLKSKLTSSERGEGIFFSSGNKYLLGYAHMKNTAWSVLVYSH
                     ADSVTTILITYAKSVLVLLIVIVITFSLFSYFIASRISIPLEKLAISTNAKDIGGSLVD
                     IKGINAWYVEADRLKKALFTHIKIMIERVDSLNEVALKDPLTGVNNRLGFSNKIQNHKQ
                     GSGASVIAIDVDFFKKINDNFGHGVGDEVLVTLAQVINCGFPGLGRFPQSRGQPERKPE
                     "
                     /name="diguanylate cyclase"
     gene            complement(9373..9621)
                     /locus_tag="NOV91_RS23600"
                     /old_locus_tag="NOV91_23600"
     CDS             complement(9373..9621)
                     /locus_tag="NOV91_RS23600"
                     /old_locus_tag="NOV91_23600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001658416.1"
                     /translation="MGERLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVA
                     RLNGIEAHREQERAAQHRKALELERSQRQRTYNGPSL"
                     /name="hypothetical protein"
     ARG             3906..4558
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1409..1955
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.652"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(2..825)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.091"
                     /coverage="100.0"
                     /evalue="1.8999999999999998e-173"
     Typing          complement(9377..10047)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.161"
                     /coverage="100.0"
                     /evalue="3.82e-113"
ORIGIN
        1 ttctcgcttt cgttcagttc cgctctgaca gactgctcgt gctcgctgaa tgcggctttc
       61 agcatctcgc cggtactctg ctgctgcgct ttcgatttct gctctaagtc cttcgccagc
      121 gttaaaagac tgctcataga tggctccctt tagtcggatg tttcgccccc cgtccgggtc
      181 ggcaatgctg atactgcttt tcgtggtgcg taccacctca aaacccgcgt tttccagcgc
      241 ctcagtaacg tcctgacggg ttttcagctc cccggacgag gctaaggcca gcaaaccccg
      301 ggtgatcgcc tgagctgcct cctgcttcgt ttccggcagc gcggacggcg tgaccagcgc
      361 ccgccggttt tccggcgcat tcgggtcgtg cagccccagc ctgccgttca cgatggtctg
      421 ccaggcatcg atgcgcggac ggtcagcgcg gtcgtaatac ggctggagcc ttttgccggt
      481 cagcagctcc gtgttcggga tcaggaagtt cagctccagc cgccccttgt cctggtgctc
      541 cacccacagc acgctgtact ggtctttatc gagtccgggc atcagaacac gctcgaagct
      601 cgccatcagt ttttcacgct gaccgggcgg taagtcctgt tcggcaaacg acagcacccc
      661 ggaggtgtac tttttggcgt agggcgaggc gtcgattagc tcccggactt cctccggttt
      721 accctgcaga acgctggcgc cgtcgcgctt gcggtcatta cccagcagat aatccaccgg
      781 accggcaccg ccgccgcgcc ctcgtgggtg aaatttaacg atcatcgtca gccccctttt
      841 ccagcacagc gtgccgcagg cgctcaagtc cggcatcaat ggccatcagc gcagcgacaa
      901 tctgtacgcg atcatgtccg gtaccaccac cggcgttaac ctgccgggca atctgattga
      961 ggttgtttcc cataccggca agttgacgaa gcaacgccgg cgagatcgac ggaagtttgg
     1021 cggcacgagc aggtttctcg tcgagacacg tctgccgcat ccaggccgcg agctgcttac
     1081 cgtcgcagcg atccagcaga cgccggtgct cgtcttcagt tacccatatc gtgagcattt
     1141 tgctgcgttt gtctgccaat ccggcctcct gataacgccg ggacggacgg ggaattattt
     1201 cccggtcggc gcggtgttgg acaagccgca ggcgcgtcag tgccttttag cgggtttcgg
     1261 ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggctt aaaatgtaac
     1321 atcgcagaaa catcaagcat agctgccaca taaagcaggt gaaaaatgag attcgtatgc
     1381 gtcagcagaa gatgcactgc cggagaaatg ccgcttcctc gctcactgac tcgctccgct
     1441 cggtcgttcg gctgcggcga gcggtaccgg cttacgggcg gggcggaaat ttcctggaag
     1501 atgctaggaa gaaacttaac agggaagcga taaggccacg gcagagccgt ttttccatag
     1561 gctccgcccc cctgacaagc atcacagaat ctgacgctca aatcagtggt ggcgaaaccc
     1621 gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt
     1681 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac
     1741 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc
     1801 ccgttcagtc cgactgctgc gcctcctccg gtaactttca acttgattcc aacccggaaa
     1861 ggcacgacaa agcgccactg gcagcagcca ttggtaactg ggaattagtg gatttagata
     1921 tcgagagtct tgaagtggtg gcctgactac ggctacactg aaaggacagt atttggtatc
     1981 tgcgctccac taaagccagt taccttcgga agaaagtgtt ggtagctctt aattccggca
     2041 aacgaaccac cgttggtagc ggtggttttt ttgtttacag ggcatgagct tacgacgaca
     2101 tcgaagggat aacaagagga tccgcttctt gaaccctttt ccccacaaaa aaacgccaca
     2161 ctagcgcgta aagcgtcatc aaaaaaactc aaatagcgct atatacaatc actttattac
     2221 gcccctctcc cttggcttga taaagagcat catcagcatt tttcagcaaa aaatcgatcc
     2281 caccaagagg atcctcttgt gttgccacac cagcagagac ggtgacccgc aagttattag
     2341 ggaaaacagt actttcaata acactcctaa tacgctccgc aacacactcc gctgttacca
     2401 cagatgtatt cggcagaaaa acaacaaact cttctccacc aaaccgacaa acaatatctt
     2461 cactacggca gcaagaatta tccggtttcg gcatgatgct gatgtagtcc cgttttatcg
     2521 tcatcacgaa gctttctgct atgccattgc tttccgggct tcgcactgct gtcgtacaag
     2581 gctccagtcc cagcaaccgg gcgaacgccg gcactgttgg caactgcctt ccgaagcgtt
     2641 tttccaccgc acccagcatg acatcctgca ccgtttcttt gtcatagcct ccggtgctcg
     2701 ctgcccaatc aatgatctcc ctgtcgcagc agtcctgcgc gaaggtcacc cgcagctttt
     2761 cgccattatc acagcggaac tcaaagccat ctgagcacca gcgccggtta ctttccgcta
     2821 ctgctacgcg ccccttatgg gcccgcttac ggcaaggatc agcgggttta cgttcaagaa
     2881 gcagattatg cgttctcata atgcgataca cccgctttgc attaaccaca ggcaggccgt
     2941 cccgctccga ctcccgtcgc agcagcgccc acacacggcg ataaccatac gtcggcaggt
     3001 cagccaccgc catgtttatc cgggacaata cggtggtatc gtcagaacgg ggctgccgtc
     3061 tgcgatcctg tcagtcagat ggccggtgaa cccgaatgct caactgcgca cgcgacacgc
     3121 caagacttct gcaaacgtcg cttattcgtc gttcccaggc aacgagggcg catgcgcaat
     3181 ccattttttt gcccggccga actccacggc ctctttgagt atctcggctt ccatcgtctt
     3241 tttgcccaga aggcgctgga gttcgcgaat ttgcttattg gcggcagcga gttcggaggc
     3301 cggcaccact tcctcgcccg atgcaacagc cgtcagtgaa ccgtcttaat attgcttgcg
     3361 ccatttgaat atctggtttg cattgatgcc atgcaggcgc gcgacatgag acacggtcat
     3421 accgggctcc atagtctgct gaataatggc aattttttcc tgcggtgtac gacgccgacg
     3481 ccgctcaggt cctgataaca cttcaaccat cttatcgttc tgactggtat taaacatagt
     3541 tccaagacta cctcttattt taagagagtc gaagtgtctg gtgatctatg gggccagtct
     3601 agccctcttc caagtgtagg gagatatagt caaagtttgc ttctccgccg atgagtccgc
     3661 cctgcgtcgg atagtccgat tttttaccgg cgaagcacgg ctctcctaac cctatctaaa
     3721 gcgaataact tttttcaaca gggccgcgtg ccaatgcaag agcgataggc gctctacgcg
     3781 ccaatttgac cacttaaaac aggtaaattg aggggttgta atgtgttgat gtaacaggct
     3841 tttattttaa tgtcttggca tatgtataat ggtagtctag ccctcctttc aacaaggagt
     3901 actcatggaa acctacaatc atacatatcg gcaccacaac ttttcacata aagacttaag
     3961 tgatctcacc ttcaccgctt gcacattcat tcgcagcgac tttcgacgtg ctaacttgcg
     4021 tgatacgaca ttcgtcaact gcaagttcat tgaacagggt gatatcgaag gctgccactt
     4081 tgatgtcgca gatcttcgtg atgcaagttt ccaacaatgc caacttgcga tggcaaactt
     4141 cagtaatgcc aattgctacg gtatagagtt ccgtgcgtgt gatttaaaag gtgccaactt
     4201 ttcccgaaca aactttgccc atcaagtgag taatcgtatg tacttttgct cagcatttat
     4261 ttctggatgt aatctttcct atgccaatat ggagagggtt tgtttagaaa aatgtgagtt
     4321 gtttgaaaat cgctggatag gaacgaacct agcgggtgca tcactgaaag agtcagactt
     4381 aagtcgaggt gttttttccg aagatgtctg ggggcaattt agcctacagg gtgccaattt
     4441 atgccacgcc gaactcgacg gtttagatcc ccgcaaagtc gatacatcag gtatcaaaat
     4501 tgcagcctgg cagcaagaac tgattctcga agcactgggt attgttgttt atcctgacta
     4561 attgctttga tgtgtgattt taaacgctca aatttataaa acgaataata ttgtcaacct
     4621 tgcaataata gacataggga caaccatgta taaattttta ccctcaggcg tcttcacaat
     4681 agagccatat agcagtgggt cacttagtgg gcttaatttt tccattaaag ataatattaa
     4741 tattgctcag tataaaacat catatggtag cccatcttgg caagtaaaca taaggctgcc
     4801 atttataagg cactgttgca aaaatgtgga ggtgataggg gtactacgcc ggaaccccag
     4861 atttttccgt ccgttcaaat tatccacggt tgaacagctt gatcccgtcc gcccgcagtt
     4921 gaccaacagg ggaaatatgc ctgtacaaag tttgccgggt tatcccaagt tcctggcata
     4981 gcgtactgac ctttgtttct gattgtccca ttgatgccat tgccagtcgc agtttgactg
     5041 gcgtcatttt ataaggtcgg ccgccgttcc gcccacgggc tcttgctgat gccagacctg
     5101 cagtcgttct ttcagcaatc agttcgcgct caaactccgc cagtgcggca aagataccga
     5161 agacaagctt gccagcggcc gttgttgtgt cgatagtcgc cccgtgaccg gtcagcactt
     5221 tcagaccggt ccccctcgcg gtcaggtcgt gcactgtatt aatgagatga cgaagatcac
     5281 gaccgaggcg gtccagcttc catacgacca gcgtgtctcc gggtcgtagt gctttcagac
     5341 aggctgtaag tccaggacgc tcttctcgtt tgcccgatgc ccggtcctca taaaaatgtg
     5401 cggcatcaac acctgcggcg atcagtgcat cacgttgtaa atcattcgtc tgcgaaccat
     5461 cagcttttga catccgaata tatccgatga acatatttcc tctgtcacat atacgttcga
     5521 ttatgtgaca ttctgatttg ggtaccataa tctgtcaacg gtagtcacat tacccgtaat
     5581 ttaaattatg acacgtaaag gttctatgtt aatgataatg tgacaagcgc gtttaaatag
     5641 gcataaggat cagagatgcc tactaaaaat aagctcctca gtattctttc ggacgccgag
     5701 caggaagcgc tctatggtct acctgatttc gatgatgcac agcggctgga gtttctggcg
     5761 ctgaatggat atgaactggc gctggcctgc agtcgccggg ggcttcacgc tcaaatttat
     5821 tgcatcatcc agatcggaga gcatgaaaaa cctcggggca gccatcgaat gatgccatct
     5881 acaggggtta aaataaccac catcatttca taacaatacc tgctgactat gaagatctac
     5941 gtcatcaaaa tagcagttca tggagtcagt ccgatggtct ggcgtcggct gagaattgct
     6001 gccgacacgt cactggccgc gcttcacttc attttccaga tagtgcaggg ctggggggac
     6061 gaccatctcc atcaatttca catttatggc aaagattacg gtatctccta cgaaggaggc
     6121 atcggttttg tcgataatcc gttccgggtc gtgattgatg attttgcttt tgatgctggc
     6181 gatcgcttta cctatgaata caatttcttt gagcactggc ttcatgacat tcgtgttgag
     6241 gccatttatg aaaactctac gctgaaagcg ccgttttgta taagcggcca tggtatgccc
     6301 ggagccacag ctgcggatga gttcgataaa accctggcgt ttcttgaagc tatcgttaat
     6361 gcggatgatg aaacaacggt cggcgagatc cgcccttttg ctgacgacct ggatgctgtc
     6421 aggttcaacc gccacaaaat caaccggcaa ctgagcaggc tcgaccttgc atctccggta
     6481 ctggagcctg aagttatctg gctgggccgt cgtcgctgac tgatgtctat aaaggtatac
     6541 gttttcagca tgcctctcag atccccgaaa aaatgtctat aaacctccct aaatcagtgt
     6601 ttatggatta ttctttaaat ctctatgatg tttaaagacc ttccggaggt tttgcgtgca
     6661 atatgcctac atcagggtaa gctcggccga tcagaatacg gcccgtcagg aagaggcatt
     6721 gtcaaaggcg ggttttcagc ctgataaaat ttgtgttgag catgccagcg cgaaggacac
     6781 aaaccgcccg gggttacagg aactcctggg gcaattacgc cctggtgata cgcttctggt
     6841 tcattccatc gatcgccttt gccggaatat gtcggatatg tgcgctgtca ccacccggct
     6901 tcgtgaccag ggcgttaccc ttatttttct gaaagagcag ctgacgttca gcgccggcac
     6961 aaacaatccg atgcaggaac tgcagctgca catgatgtcg gcattcagcc agtttgaacg
     7021 agccttactg aaggaaaggc aggccgacgg catcgccgcg aaaaaagcac ggggtgaaaa
     7081 aacagggcgc cccggcgccg atatcaaaaa gatccaggaa attgacgcac tcagaagcag
     7141 aggggtcagg ctcaggatcg cctgcgacca tgcgggcctt ggcgtttcca catattataa
     7201 gcttcgtcac cagatgaaca accagtagcc gggagatagt catccaccac agcaggaggc
     7261 aatgatgcaa ctgacgctcc agatagtcat caccgatgaa tcaggctcca gtcgaacgga
     7321 ggaactgatg acaatccaga aatcagggga gacccggaac gacatcggat tatcggtgtc
     7381 agaatccaag ctgttgctga atacggtcca gcagtcggtg gtccagctgc aggcagacga
     7441 atatactcaa caccatatcc ggtgccctca ctgccttgct gcgcgcagaa tcaaaggcaa
     7501 acagaaaata cggtaccgga cgctgtttgg cgttattccg gtatccgggc ttcgggtgta
     7561 ccggtgccgt tgtgaagaga gtgatacaaa gacagtcagt ttgctcagcg actgggccgg
     7621 cgattattct cacccggcac tgaaatatat tgaaacccgc tgggcctcga tgatctccta
     7681 tgaaatgacg actcgtctgc tgaaagatat tctgccggtg ggccacagcc tgaatgcctc
     7741 aacggtgagg aatcatttat gccaggtggc gcagcgtctt gacgctgagg ctgaagctca
     7801 ttcaggtttt ctttccggct gcccccgcga ctgggggaac ctgcccaggc caggaaaacc
     7861 gcaattaata acctgagcca aagtaacaag aacctcatct ccgaccccat gcccgaagtt
     7921 atcatttatt tttttaaaaa aatcaacatc aatagcaata actgaagccc ctgaaccctg
     7981 cttgtgattt tgtattttat ttgaaaaccc cagtctgttg ttcacgccgg ttaggggatc
     8041 tttaagcgcc acctcattta gagaatcaac tctttctatc attatcttaa tatgcgtaaa
     8101 aagtgccttt tttagtcggt cggcttcgac ataccaagca ttgatacctt taatatcaac
     8161 tagcgaaccc cctatatctt tagcattggt agatatagca agcttttcta aaggaattga
     8221 aatacgggaa gcaatgaaat atgaaaaaag tgaaaatgta ataactatta caattagcag
     8281 aacaagtaca ctcttagcat aagtaataag aatagtagtc acgctgtcag cgtgactata
     8341 aaccaacaca ctccaagcag tatttttcat atgagcataa ccaagaagat atttattccc
     8401 cgaagaaaaa aagataccct cacctctctc agacgaagtt aacttcgatt tcaaattatc
     8461 atccataacc attggtcgac caacagcact gttatcgtta ttaaagatca catttccatc
     8521 atcactaaca atgctaattt cagtgtcgct tttgaaaaaa tgcctgctca aaacgtcgct
     8581 aaataaactc tgttccttta gatatataga cccgccgaca taccctatat aattactatt
     8641 cttatcataa ataggatgtg acaaaaaaat aatcaaattc ccagaagctg atttgtaagg
     8701 tgccgatata aatggttttt tagcctcaat cgccaactta ctggcacttg aattcaagtg
     8761 aactccaact aaaccaagtg attctggcga agtcgccaga acaacagcac catcactaac
     8821 aacaagaact gaattaaaca taccagactg caatcttaac ctatccgcct catttttaag
     8881 tgagtcaata tcattagttg actctatttt ttttgcgcca tacgccaatt cacgttgagc
     8941 aatttcaata taattactca ttacatctga cattttcata gcgtacgatg aattagcatc
     9001 aagcaagtct ttttctatat tttcgcgttt aaaaaccata ataacgacaa tcagaaaaat
     9061 agaagtcaac aaaataccac caacggacaa ggttatgact atcggacgaa ggtgaggctt
     9121 tttaattctc ataaaaatac actctatata attaaaaaat tccacattta ccataacatc
     9181 ccttgcccgc accgccccgg acgggttcgg cgcgaatgcg gcgtcagccc ggtaaaaccg
     9241 gcagtaaact ccccgctaaa ccgcgctttt tgagccagat aaatttcccc aagatgcata
     9301 gccatgcagc cggggggatg gcaccgcctt tcggcgggga tctgcgccga aagacccgtt
     9361 ttatccggtt tgttacagcg acggaccgtt gtacgtccgc tggcgctgcg atcgttccag
     9421 ctccagcgct ttccggtgct gcgccgcccg ttcctgctcc cggtgcgcct cgataccatt
     9481 cagccttgcg acaaccggcg cagctgctcg ctcaaactct gcacctgcct gctcaagccc
     9541 gtgacgcgct cgctcagcgc cgcgttctcc cgttgcataa gaccagacat cgtccgccat
     9601 tccgcgaagg cgttctccca ctcgtccagc cttttcgagt agtcctgctg tagctgctct
     9661 aatgcgctca gcaactgttt ttccagctcc gtcatgtgct atttccccgc cagtatcatc
     9721 cagctcgaat cctctccgaa ccgtcccgct tccgggttca ccctcacgca gctgcgtctc
     9781 tgctcgccgc aggtcgagag ctgcacgccg ctgttccgct cggacagcat cgcctgcgtg
     9841 ctcttctgct cccggatggt cgtgtaattg tcgagtatct gctgactctg ccaccacaga
     9901 atgcccgcgc tcgatgcgat cagcaggacg gaaaccagga cgatggtcag ccacgtctgg
     9961 ctgaccatac gcaccatgcc tttcgtgcgc tggctcatgg ctgaggacag cttccggtcg
    10021 tggtcgagga tggcggcgct gattctc
//
