LOCUS       NZ_CP101570            10060 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KP29 plasmid p29_3,
            complete sequence.
ACCESSION   NZ_CP101570
VERSION     NZ_CP101570.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19931444
            Assembly: GCF_024397235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,P.
  TITLE     Retrospective study on epidemiological characteristics of KPC-2 and
            NDM-1 producing Klebsiella pneumoniae in Kunming, Yunnan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2022) Department of Key Laboratory, Second
            Affliated Hospital of Kunming Medical University, Dianmian road No.
            374, Kunming, Yunnan 650101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUL-2022
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 190x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 06:13:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,863
            CDSs (total)                      :: 5,742
            Genes (coding)                    :: 5,550
            CDSs (with protein)               :: 5,550
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 192
            CDSs (without protein)            :: 192
            Pseudo Genes (ambiguous residues) :: 0 of 192
            Pseudo Genes (frameshifted)       :: 85 of 192
            Pseudo Genes (incomplete)         :: 120 of 192
            Pseudo Genes (internal stop)      :: 20 of 192
            Pseudo Genes (multiple problems)  :: 29 of 192
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101570.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP29"
                     /isolation_source="secretions"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p29_3"
                     /geo_loc_name="China:Kunming"
                     /lat_lon="25.05 N 102.68 E"
                     /collection_date="2013-04"
                     /collected_by="Hu Ying"
     gene            1..279
                     /locus_tag="NMY30_RS29195"
                     /old_locus_tag="NMY30_29175"
     CDS             1..279
                     /locus_tag="NMY30_RS29195"
                     /old_locus_tag="NMY30_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            267..581
                     /locus_tag="NMY30_RS29200"
                     /old_locus_tag="NMY30_29180"
     CDS             267..581
                     /locus_tag="NMY30_RS29200"
                     /old_locus_tag="NMY30_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            745..1173
                     /locus_tag="NMY30_RS29205"
                     /old_locus_tag="NMY30_29185"
     CDS             745..1173
                     /locus_tag="NMY30_RS29205"
                     /old_locus_tag="NMY30_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1199..1378
                     /locus_tag="NMY30_RS29210"
                     /old_locus_tag="NMY30_29190"
     CDS             1199..1378
                     /locus_tag="NMY30_RS29210"
                     /old_locus_tag="NMY30_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1405..1935)
                     /locus_tag="NMY30_RS29215"
                     /old_locus_tag="NMY30_29195"
     CDS             complement(1405..1935)
                     /locus_tag="NMY30_RS29215"
                     /old_locus_tag="NMY30_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1942..2673)
                     /gene="mobC"
                     /locus_tag="NMY30_RS29220"
                     /old_locus_tag="NMY30_29200"
     CDS             complement(1942..2673)
                     /gene="mobC"
                     /locus_tag="NMY30_RS29220"
                     /old_locus_tag="NMY30_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2673..4637)
                     /locus_tag="NMY30_RS29225"
                     /old_locus_tag="NMY30_29205"
     CDS             complement(2673..4637)
                     /locus_tag="NMY30_RS29225"
                     /old_locus_tag="NMY30_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6121..6270)
                     /locus_tag="NMY30_RS29230"
                     /old_locus_tag="NMY30_29210"
     CDS             complement(6121..6270)
                     /locus_tag="NMY30_RS29230"
                     /old_locus_tag="NMY30_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6355..6612)
                     /locus_tag="NMY30_RS29235"
                     /old_locus_tag="NMY30_29215"
     CDS             complement(6355..6612)
                     /locus_tag="NMY30_RS29235"
                     /old_locus_tag="NMY30_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(6622..8307)
                     /locus_tag="NMY30_RS29240"
                     /old_locus_tag="NMY30_29220"
     CDS             complement(6622..8307)
                     /locus_tag="NMY30_RS29240"
                     /old_locus_tag="NMY30_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8634..8879
                     /locus_tag="NMY30_RS29245"
                     /old_locus_tag="NMY30_29225"
     CDS             8634..8879
                     /locus_tag="NMY30_RS29245"
                     /old_locus_tag="NMY30_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9153..9524
                     /gene="tolA"
                     /locus_tag="NMY30_RS29250"
                     /old_locus_tag="NMY30_29230"
     CDS             9153..9524
                     /gene="tolA"
                     /locus_tag="NMY30_RS29250"
                     /old_locus_tag="NMY30_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            9521..9886
                     /locus_tag="NMY30_RS29255"
                     /old_locus_tag="NMY30_29235"
     CDS             9521..9886
                     /locus_tag="NMY30_RS29255"
                     /old_locus_tag="NMY30_29235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1622..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5440..6018
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1946..2673)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atgtcatcag caacatccat caggctcgat gaagaactta aggaccggct gaaaacgctg
       61 gccgatgacc ggcaccgctc agcgcacgcg ctaatgctgg aggcaatcac cgaatacatc
      121 gaccgggaag agaagcgcag ccagtacctg cgggacggac aggccgcatg gcagcactat
      181 caggaaacgg gtctgcacct tactgcggaa gaagcggaag cctggataag tacctgggga
      241 actgagaacg agcaggatgc tccgccatgc cacaggtaac gatttccgcg ctggcacagc
      301 gcgatttaca acgccttcag gactttctca aaaccaaaaa tcggctggca gccagaaaag
      361 gaggtgaggt gatcgtccgg gctatccagc aactgaaaac attgccagac attggccgcc
      421 cggtgccgtt tctgccgctg gaatataagg aactggtgat cgggtttggc gacagtggct
      481 atgtgatgct ctaccgccac gaccgggaaa tggaccagat tgtgatcgtc accgtcaggc
      541 atcagaaaga atccgggtat ccgggggcag acagcctcta ataaccgatg acgggggcgc
      601 aattcattgc gtggccgtcg ccggtttgac aagcgcagcg cgtcagaaaa ccacatcctg
      661 aacggaaaaa cgggtaaaat tcagcgatac atttaccgtg agaaattaca cacctttcac
      721 acccgataaa atcaggggta tacagtggcg aaaacattgc tcaacctttc acaggcagca
      781 caagccgcgg gcattaccag aagaacgcta tacaatcatg taaaacaggg aaaggttacc
      841 gtgtcacgtg acgggaaaaa caaccctgtc gtggatgtgt cagaactgat ccgtgtatac
      901 ggaaatgtga atatccctga gaaacaaatt cccggcattt cacaccgtga aaatacacaa
      961 aaaaatttcc cacaggaaca gcttctggca atgcaaaagg aactggccga tctccggcag
     1021 gctgtcaccc tgatgctgga agataaaaca tcccgggaag aagaacgccg ccagcacgat
     1081 gatgagcgcc ggaaactcca ggccgaagtg gacagattga caacagaact gacacagaag
     1141 aaaaaacgct tctggtccgg ctggtttagc tgagaactta ccgaagggaa tggagaacat
     1201 gacgaagcag gaaaaaactg ccctcaatat ggctaaattc attaaggatc agtcgctgct
     1261 gttgctggaa aagctgaacg agctggacat ggataccccg gccgacatgt gcgaacggct
     1321 tcacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg aagaataaca
     1381 gcaccggcca tgccggtgct ggcgttattt tttgccgaac ggccaccacc ggcgggcggg
     1441 tttgtcctcc ggcagttcag gcaccggcac gttaaaatag cggctgttat attcccgcat
     1501 cagctccgct ttcagcttct cgttcaccgc gctttccccg tgccgctcgc tgaccacctc
     1561 aatcatcgcg ttaatctgct ccggtgagca cttcccggca tcaaccacct gcacaccgtt
     1621 ctcgatggcg aaccgctcca cctttcgata gaggtaatca cacagcttca ttcctgtggg
     1681 tttgtcccgt tcccattgcg gattttcacc ggtgaaaaag tacgtggctt tttcgtcgct
     1741 gttgagccgc tcgtcataca tcccgatgga tttcatcatc gccagatgct gggcccggtc
     1801 gacaaaacag aaaacatacg ccccggtctg ccaggcgagc accaccatgt cttccagatg
     1861 ctcgctggtt ggcataatgc agcgctcgcc gtctttgtct tccacgtcct caataaatac
     1921 catgtacagc gccataatcc ctcactccgt tgtcataaat ttaaagcacg cgaacagcct
     1981 ctcccggtcc agctccaccg ttttgcgggc ctcgccggtc tgtggattca cgatttcatc
     2041 caccgccaga atcgcccgct gtacccggac aagcgtcggc cggtcagggc agatgtacca
     2101 gatttcatcg atgccgttcg cccggcggtt gaacagatac ccggacacca cttcctgata
     2161 ccgcctgcgg ctcttgacgg tgcgctctac ctcaaatgcc accgttctgc cgtccagctc
     2221 cgccagcgcg tccggcactt tcatcccttt cagggccata cggtgcaggt accgccagcc
     2281 ggtggccccc cggccctcca tggagaggcg cagggactgc acggccagcc ggtgcggcag
     2341 ctgcgccgcc gacacccttg acggctgaaa cggaataacg tccgtcagcg gctcgtcctc
     2401 gtcgaacgag aacgccaccc cgtgcggcgt cagcccccac acgctgacct gacggccgtc
     2461 aaccgggtat ttcgcccgga ccagcagccc tttcgccacc agactgtcca ggaagcggta
     2521 aaccggctgt accgccgcaa tccccagccg tcgcgcaacc agctccgccg tggaacacag
     2581 ctcttcccgc agccagcgca gggtcagccg tgttttctcc gcgttccgcg cctgccgctt
     2641 cgcgtggctg acgttgtact gctccagtgt catcagtaaa gccccgcaaa atcatcatcc
     2701 gcttttggtc cgccgccgtc ggccggaggc aggtactccg ccagcgggtc cgttttcgcc
     2761 agcaccggga aggttttcag cgaaatttcc cggcgctcaa cccgcagcgg gctgctgaac
     2821 gccagccgcg ccacgcccag cccggtcatc accgcgaacc cgtcaggcag gtgttgcagg
     2881 gtgttcacgt caaacagcgg ccgcgcctcc ttctggatga agctgtcgcc gctgatgtgc
     2941 tccacgttcc ccgcctcccg gctggcccgg cggcgctcca cgtccacccg gatctccccg
     3001 gtctggcccg ccgcccactg cgcggactcc tggctggccg cgcgatagaa ccagcggatc
     3061 ggcgtgttat ccagtaccgt ggttttcaca aaatcagcgg gcagatcctg cccgcactga
     3121 ccgaagtcgc ccagcgactg gtgcgcgagc agcaggttgc agtcccggct gcgcagcgtc
     3181 cccagcgcgt tcagcacgta cttcgacagc agatatttaa tctcatcgag catgatgctc
     3241 gcgtgcggcc acctccggaa ctcatcccgc gcaatgatga tttgcgcgca gcgggcaaac
     3301 agcatcttct gcacccggat caccgcctca tcgtccatcg agccgatgac gtacaggcag
     3361 ccgccgccgt tgattatccc cgccacatcg ataccgctgt ccgtctgcag gacgctcagg
     3421 tcggccacgt tctccagctt ggtgatcagc cccttcacgt cctttttcag ggcctccggc
     3481 aacgcgtacg ccgccgccag cacctgctga atgtttgccc cctgcgggaa ctgctccgca
     3541 atcagcttcg ccgctttctg ctcgctgatg cggtagtggt ccgccacgtc gccggtctca
     3601 ctcaggttaa acccggcaac cagcagattc ttcagggcgt actggtcgag atcgcggaac
     3661 agattcagct gcgccggttt ccccggccgc agatccagca gggtgaagtc cgggcagtgc
     3721 gcccggaaga cgtgcggcgc ccacgcgtcc cctttggggt cgaagtacac cacagcgtcc
     3781 ccgaaatcgc gtacgcagcg aatgagcgcg ttggtggcca tcgcgctctt gccgctcccg
     3841 ggcaggccga ggatctgaat attggtcttg cgccaggtat cacgagggac cagcaccggc
     3901 tggccctgct catccttacc cagaaacacc gcctgctgtt tccgggcctg ctccgtggaa
     3961 aaatcggcca gcgcgttaac cggtaccggc tccccgaaat gcaggtggcg gacgtctgtc
     4021 atttctccgg tccgctggct gctgcgccgg ctctcctgat tcagtttcgc gttcaccgcc
     4081 tcgtcagcgg ccgcaaaatg accatcaagc agcaggaacg ccagcagccg cgccagaagg
     4141 acgccggcga caagccctcc ggcacaccag gctatcccga acaggttagc cggctgcagc
     4201 aggtaataat ctgcgttcgc atagaggtcc gggcgcagga cggtttctcc ccagctctcg
     4261 ataaacacgg cgacaaccag cccccccaga agggccagac cggcagcgcc ggtcagccag
     4321 tacgcgtagt tccagacatg tcgccgtgcc ctgaccatcc gcccgggtga atccagcgag
     4381 gccagcaccg aaaatggcac agtccggtta ttatcgcgct tatagcgatc ggcatactcc
     4441 cgccgcgcct tatggctcag ccccacaaaa cgcattcggc cggcccagag ttcagccaga
     4501 ccgaaaaaca ggccgataac cagaaaaaaa ccggccagac tgtaatactc ccggtacagc
     4561 cagctgaccc acgggtaaat ctgccgggcc agctccggga tatgccactg ccaggcaagc
     4621 gaatccgtat taaacatgca atctctctcc ttcagacgac gggtttaccg gcgtgatgcg
     4681 cagcccccgc tgcacaagcc gcggacatgc cccaaggcat ggcggcggcg gtcacgtccg
     4741 tggtgattgc gttcagcttt caccgcggag gccagcctct ttccgcccca tgcggaaaag
     4801 gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag gcggcacccc
     4861 agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga gcggctgacc
     4921 acccgcaaaa tcaggagcag ctttgctgct tcgtcaggtg cgtccagcac cgtggatttt
     4981 tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt ggccggcgtc
     5041 cagccggaca gagccagaaa gaggggaaaa gaccgtgcgt cggtggcgtc cagccagcgt
     5101 aagcaaggtc ttttcccctg aagtcagcca cgccccagcg gggatgactt gcatttctcc
     5161 gcaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa aaaccgacat
     5221 ggttcaccta tcctgataat tgatcgtcag ggaatagaaa gacttaatca ggggacaata
     5281 tcccacatca gcgcgtccgg tgaccgtctt gcaggtgccg gggaaattgc gcctcctcgc
     5341 tcactcggtc gctacgctcc gggcgtgaga ctgcggcggg cgctgcggac acatacaaag
     5401 ttacccacag attccgtgga taagcagggg actaacatgt gaggcaaaac ggcagggccg
     5461 cgccggtggc gtttttccat aggctccgcc ctcctgccag agttcacata aacagacgct
     5521 ttttcggtgc atctgtggga gccgtgaggc tcaaccatga atctgacagt acgggcgaaa
     5581 cccgacagga cttaaagatc cccaccgttt ccggtaggtc gctccctctt gcgctctcct
     5641 gttccgaccc tgccgtttac cggatacctg tccgccgttc tcccttgcgg gaagtgtggc
     5701 gctttctcat agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg
     5761 ggctgtaagc aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca
     5821 cttgagtcca acccgggaaa gcacgcaaaa cgccactggc agcagccatt ggtaactggg
     5881 agttcgcaga ggatttgttt agctaaacac gcggttgctc ttgaagtgtg cgccaaagtc
     5941 cggctacact ggaaggacag atttggttgc tgtgctctgc gaaagccagt taccacggtt
     6001 aagcagttcc ccaactgact taaccttcga tcaaaccacc tccccaggtg gttttttcgt
     6061 ttacagggca aaagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     6121 ctactgaacc gcgatccccg tcagttcaga ggaggacgat ggtgccaccg ttccgccctg
     6181 aacatcccgg atataatttg cctgacatgc ggccagaaga aagccagcaa caatcaatat
     6241 aaaaagaaaa atagtcttta cctttttcat atactttccc tgtatttaaa gactccggca
     6301 ttcatccctg aacaccggaa gtcagtattt caaatcatcc ttgataattt tttgttacca
     6361 gttaccgtcc cgataatcaa aagctatata acaattatcc acatcaggtt ttatttgatt
     6421 tttaaaaaat ggctgtagta aaggaaccca ttctttcttc aaatcaaact caccattatt
     6481 aatattatcc tttaaaggca ttcccaagct ttcaatgaca gaaccatcgt caccaaagtc
     6541 tttagaatac tcttcccctt taaactcttc agttttctta tcaaaccatg tcaaacgtaa
     6601 tttaagcccc atatttacct ctcaaagata ttttttaatg ttccgttttg gatctggccc
     6661 tttaacctgc ttacccgttt tgggatcaaa tgcgcctagg tgttcgccat cactggcacg
     6721 atacccttca agctcaccgt gctgggaatc ccattcataa attttacgtt ttttatcgcc
     6781 ataccatcgg gcccgcttac caccaccacc ttgtttaggt gttttaggat cacctcgctt
     6841 taactctcca agtcccttta tgtcttctgt tttaggtact ggatgataat catggccgta
     6901 gtctttagtc cccttgcgag gcttgttctt ttcctcattt aacttatttt ctgcatcctt
     6961 cgcttttttc tccttttgtt tccggctctc catcgccccg gtaagtgcag ccttatctct
     7021 ggataaggat tcattcattg cgtttaaaga tgcctgagcc gcttttaatt tctcattctg
     7081 attctgaata tccttatcaa gatagcgaac ctgatcctgc cacccggagc catattcatg
     7141 aggcgggtta taagtcacaa aatcatcttt atttgcctct gcctctttta cctttttatc
     7201 ggcttcctga acagcagccc ttgcaccggg gagcgccttc tctgtcgatg caatgcggct
     7261 gtttaaggtc gcaatatttt tatcttcggc atccagctca gctttcgctt tatcatagtc
     7321 tcttttcagc ccctcttccg gatgagcggc gtcccatgcc tgctggcgac gcttttcttc
     7381 ctcctgacgc tgttttacct gtgccggggt aagaacatct gtcaccgaca cataaaccgg
     7441 cttctgtccg gtctctttcg ggaaacgaat aacggcctca cgggagttac caccggctgt
     7501 aaaaccagcc ggacgtgaat cacctttttc agcaacaatg ccttttggcg gggctttcgc
     7561 tgtcggaaca cctttcggta cgctcacctg cagagacggg agacccggaa tcgacacact
     7621 gaataccccc ggacgttttg tcggtttcgc atccaccaca gggacactca ttggccgacc
     7681 tgcgacaacc gcaatatgct gccgctcatc cttcaccaca tccacaaccc gttgtctgac
     7741 acgaaccgtc gcctggtcca gtggtaaagt acttgccgga gtctccgtca ccgcatcggc
     7801 cggtaatgac gtcataattt ttgacatcat tttcggatca tcttttgcaa tctcagaagg
     7861 cagcacaccg tagatcgcaa tcccccacag accaaactta aacggcccct tcagggcagc
     7921 cagcacatca gcaacggcgg ccgacaacgc atcgccggaa actgataaag ccagtccttc
     7981 agcccccgga gtagccaggg caggcagacc gaaggccatc gcagtggccg aagactgtcc
     8041 gccaccagag ctaccgctat gattgccgct gttaccacca ttaccaccgg gtttactccc
     8101 gccgttacca aactcccccg gattatagtt atgaatatct ccattcggcg tatgcgtcgt
     8161 accccagccc ggcccgctgt ttggatccgt accaccacct gatgaagggc cacctttacc
     8221 agacgtaccg ttcacattcc cactggcctg accaccatta tgtcccagac ctgaattacc
     8281 cggacctcga ccgtctccac cactcatacc gttttcctct ttgacattta aaacaataaa
     8341 ttaaagccaa catactgtac atatacacag tataattatt accttactcc tcccgttttc
     8401 aggtgtcaat atatgagatt agttctcatc ttcaggcaca aaaaagcgcc cggagccacc
     8461 tgtaagcaga tcgcgtcttt tcccgaaaat ttctcatggc aacaaatttt attatttaag
     8521 acattcatca cacttacatt tcagatggta tagacacatc tgaaagctaa ctggcatgag
     8581 gagaggacct gcggttaaac tgttcgggaa acctgagacg cccggaggcc actatgacgc
     8641 acctgaaact gactcccgct gaagaaaagc gcctggcgga cgcctttgtc gctcaggcac
     8701 tggccatggg cctgaaaggg aacaaaacgc tgccaaaggc ggtaaaacaa caggtgcggg
     8761 aaagggtgct tcgtgccatt caggacgaac gtgaagccag gcgggcgcag gaaatgcgtc
     8821 gggagaaata cgaggctacg gaaaccacct tcacctggca gccacagcgc cgccggtaag
     8881 gggtatttac cataacgtcg cattgcccgc accgctcaga cggtctgagg gcgtttccgg
     8941 cggctacggg ataaaacagg caacaaacca cccggaaaac cgccggaaac gcgcccggta
     9001 aattgtaacc cgctgcatgc ctatgcacgc aactgcatgg cgggccgttt ggtgtaaaaa
     9061 cgatacgata agccgtgttt atgtaaatga acgtggccgt aaagagtgac aaatatacat
     9121 gtaacatttt ttactgacag gtcatcaacc cgatgcgttt aatacccttt atcctgctgg
     9181 cattgtgcag ccccttactc ctgacggcct gtaagcattc caccacccag ccgccgaaaa
     9241 tttatactct ggataagtca tctaaaatca gtagctacgc tgcggccatc aagaccgctg
     9301 ttgaaatgca gatgtttgaa ccagaggcct atgcgggaaa aatgtgcacc gtgcggatat
     9361 ccatacaacg tgatggcact ctgaacagcg ccattgctga aggaggagat ccgaaactct
     9421 gtaaagcagc aatatcagcc gttacccacg ccaaaattcc gccggcaccg gatgaaaata
     9481 cctggcaaat attcaaaaac gcccctctgg atttcagacc atgaaagctt ttactctgac
     9541 agtgctggcg gccatgttcc tcactccggt acatgcgata ccactaaaac ccgtaaccac
     9601 actgcagcca acctacccgg tacaggccgc agcaaaacgc atcgaaggtc aggtgaccgc
     9661 cacgtttgat attctggacg atggacgagt ggacaacgtg tcgcttaccg gctgtcacct
     9721 tttttttcgg gaaacgctga cggcaatgag gcactggcta tatcccgcag ggcacccggc
     9781 aatgcgacag gttataaatt ttgaattcag tctgaaaaat gacggcttac aggatccgcg
     9841 accagtcacc acccaaaagc tcaatgatga tggttgccgc cagtaacagg tcctttaacc
     9901 cgcaggctgc aatagtcctg gatgatgagt gagcctgtag cagccctgat tagtggaagc
     9961 gaagctgaaa aatgggcagc aaaagccgta tttctggcaa atagcgccgc cacggattaa
    10021 agtagtcatg tgtaatacat atgactacaa aggggctttt
//
