LOCUS       NZ_CP101565            10060 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KP65 plasmid p65_2,
            complete sequence.
ACCESSION   NZ_CP101565
VERSION     NZ_CP101565.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19931463
            Assembly: GCF_024397075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Wang,P.
  TITLE     Retrospective study on epidemiological characteristics of KPC-2 and
            NDM-1 producing Klebsiella pneumoniae in Kunming, Yunnan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Wang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2022) Department of Key Laboratory, Second
            Affliated Hospital of Kunming Medical University, Dianmian road No.
            374, Kunming, Yunnan 650101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUL-2022
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 180x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 05:57:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,559
            CDSs (total)                      :: 5,439
            Genes (coding)                    :: 5,315
            CDSs (with protein)               :: 5,315
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 50 of 124
            Pseudo Genes (incomplete)         :: 75 of 124
            Pseudo Genes (internal stop)      :: 14 of 124
            Pseudo Genes (multiple problems)  :: 15 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101565.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP65"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p65_2"
                     /geo_loc_name="China:Kunming"
                     /lat_lon="25.05 N 102.68 E"
                     /collection_date="2013-04"
                     /collected_by="Hu Ying"
     gene            join(9795..10060,1..13)
                     /locus_tag="NMY31_RS27680"
                     /old_locus_tag="NMY31_27660"
     CDS             join(9795..10060,1..13)
                     /locus_tag="NMY31_RS27680"
                     /old_locus_tag="NMY31_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1..315
                     /locus_tag="NMY31_RS27685"
                     /old_locus_tag="NMY31_27665"
     CDS             1..315
                     /locus_tag="NMY31_RS27685"
                     /old_locus_tag="NMY31_27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            479..907
                     /locus_tag="NMY31_RS27690"
                     /old_locus_tag="NMY31_27670"
     CDS             479..907
                     /locus_tag="NMY31_RS27690"
                     /old_locus_tag="NMY31_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            933..1112
                     /locus_tag="NMY31_RS27695"
                     /old_locus_tag="NMY31_27675"
     CDS             933..1112
                     /locus_tag="NMY31_RS27695"
                     /old_locus_tag="NMY31_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1139..1669)
                     /locus_tag="NMY31_RS27700"
                     /old_locus_tag="NMY31_27680"
     CDS             complement(1139..1669)
                     /locus_tag="NMY31_RS27700"
                     /old_locus_tag="NMY31_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1676..2407)
                     /gene="mobC"
                     /locus_tag="NMY31_RS27705"
                     /old_locus_tag="NMY31_27685"
     CDS             complement(1676..2407)
                     /gene="mobC"
                     /locus_tag="NMY31_RS27705"
                     /old_locus_tag="NMY31_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_255846784.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLASLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2407..4371)
                     /locus_tag="NMY31_RS27710"
                     /old_locus_tag="NMY31_27690"
     CDS             complement(2407..4371)
                     /locus_tag="NMY31_RS27710"
                     /old_locus_tag="NMY31_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_255846785.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYDIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5855..6004)
                     /locus_tag="NMY31_RS27715"
                     /old_locus_tag="NMY31_27695"
     CDS             complement(5855..6004)
                     /locus_tag="NMY31_RS27715"
                     /old_locus_tag="NMY31_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6089..6346)
                     /locus_tag="NMY31_RS27720"
                     /old_locus_tag="NMY31_27700"
     CDS             complement(6089..6346)
                     /locus_tag="NMY31_RS27720"
                     /old_locus_tag="NMY31_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(6356..8041)
                     /locus_tag="NMY31_RS27725"
                     /old_locus_tag="NMY31_27705"
     CDS             complement(6356..8041)
                     /locus_tag="NMY31_RS27725"
                     /old_locus_tag="NMY31_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8368..8613
                     /locus_tag="NMY31_RS27730"
                     /old_locus_tag="NMY31_27710"
     CDS             8368..8613
                     /locus_tag="NMY31_RS27730"
                     /old_locus_tag="NMY31_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8887..9258
                     /gene="tolA"
                     /locus_tag="NMY31_RS27735"
                     /old_locus_tag="NMY31_27715"
     CDS             8887..9258
                     /gene="tolA"
                     /locus_tag="NMY31_RS27735"
                     /old_locus_tag="NMY31_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            9255..9620
                     /locus_tag="NMY31_RS27740"
                     /old_locus_tag="NMY31_27720"
     CDS             9255..9620
                     /locus_tag="NMY31_RS27740"
                     /old_locus_tag="NMY31_27720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             1356..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5174..5752
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1680..2407)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.4299999999999999e-139"
ORIGIN
        1 atgccacagg taacgatttc cgcgctggca cagcgcgatt tacaacgcct tcaggacttt
       61 ctcaaaacca aaaatcggct ggcagccaga aaaggaggtg aggtgatcgt ccgggctatc
      121 cagcaactga aaacattgcc agacattggc cgcccggtgc cgtttctgcc gctggaatat
      181 aaggaactgg tgatcgggtt tggcgacagt ggctatgtga tgctctaccg ccacgaccgg
      241 gaaatggacc agattgtgat cgtcaccgtc aggcatcaga aagaatccgg gtatccgggg
      301 gcagacagcc tctaataacc gatgacgggg gcgcaattca ttgcgtggcc gtcgccggtt
      361 tgacaagcgc agcgcgtcag aaaaccacat cctgaacgga aaaacgggta aaattcagcg
      421 atacatttac cgtgagaaat tacacacctt tcacacccga taaaatcagg ggtatacagt
      481 ggcgaaaaca ttgctcaacc tttcacaggc agcacaagcc gcgggcatta ccagaagaac
      541 gctatacaat catgtaaaac agggaaaggt taccgtgtca cgtgacggga aaaacaaccc
      601 tgtcgtggat gtgtcagaac tgatccgtgt atacggaaat gtgaatatcc ctgagaaaca
      661 aattcccggc atttcacacc gtgaaaatac acaaaaaaat ttcccacagg aacagcttct
      721 ggcaatgcaa aaggaactgg ccgatctccg gcaggctgtc accctgatgc tggaagataa
      781 aacatcccgg gaagaagaac gccgccagca cgatgatgag cgccggaaac tccaggccga
      841 agtggacaga ttgacaacag aactgacaca gaagaaaaaa cgcttctggt ccggctggtt
      901 tagctgagaa cttaccgaag ggaatggaga acatgacgaa gcaggaaaaa actgccctca
      961 atatggctaa attcattaag gatcagtcgc tgctgttgct ggaaaagctg aacgagctgg
     1021 acatggatac cccggccgac atgtgcgaac ggcttcacga ggatgcggaa acactctggc
     1081 aggtgatgcg ggataagctc ggggaagaat aacagcaccg gccatgccgg tgctggcgtt
     1141 attttttgcc gaacggccac caccggcggg cgggtttgtc ctccggcagt tcaggcaccg
     1201 gcacgttaaa atagcggctg ttatattccc gcatcagctc cgctttcagc ttctcgttca
     1261 ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat cgcgttaatc tgctccggtg
     1321 agcacttccc ggcatcaacc acctgcacac cgttctcgat ggcgaaccgc tccacctttc
     1381 gatagaggta atcacacagc ttcattcctg tgggtttgtc ccgttcccat tgcggatttt
     1441 caccggtgaa aaagtacgtg gctttttcgt cgctgttgag ccgctcgtca tacatcccga
     1501 tggatttcat catcgccaga tgctgggccc ggtcgacaaa acagaaaaca tacgccccgg
     1561 tctgccaggc gagcaccacc atgtcttcca gatgctcgct ggttggcata atgcagcgct
     1621 cgccgtcttt gtcttccacg tcctcaataa ataccatgta cagcgccata atccctcact
     1681 ccgttgtcat aaatttaaag cacgcgaaca gcctctcccg gtccagctcc accgttttgc
     1741 gggcctcgcc ggtctgtgga ttcacgattt catccaccgc cagaatcgcc cgctgtaccc
     1801 ggacaagcgt cggccggtca gggcagatgt accagatttc atcgatgccg ttcgcccggc
     1861 ggttgaacag atacccggac accacttcct gataccgcct gcggctcttg acggtgcgct
     1921 ctacctcaaa tgccaccgtt ctgccgtcca gctccgccag cgcgtccggc actttcatcc
     1981 ctttcagggc catacggtgc aggtaccgcc agccggtggc cccccggccc tccatggaga
     2041 ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc cgccgacacc cttgacggct
     2101 gaaacggaat aacgtccgtc agcggctcgt cctcgtcgaa cgagaacgcc accccgtgcg
     2161 gcgtcagccc ccacacgctg acctgacggc cgtcaaccgg gtatttcgcc cggaccagca
     2221 gccctttcgc caccagactg gccaggaagc ggtaaaccgg ctgtaccgcc gcaatcccca
     2281 gccgtcgcgc aaccagctcc gccgtggaac acagctcttc ccgcagccag cgcagggtca
     2341 gccgtgtttt ctccgcgttc cgcgcctgcc gcttcgcgtg gctgacgttg tactgctcca
     2401 gtgtcatcag taaagccccg caaaatcatc atccgctttt ggtccgccgc cgtcggccgg
     2461 aggcaggtac tccgccagcg ggtccgtttt cgccagcacc gggaaggttt tcagcgaaat
     2521 ttcccggcgc tcaacccgca gcgggctgct gaacgccagc cgcgccacgc ccagcccggt
     2581 catcaccgcg aacccgtcag gcaggtgttg cagggtgttc acgtcaaaca gcggccgcgc
     2641 ctccttctgg atgaagctgt cgccgctgat gtgctccacg ttccccgcct cccggctggc
     2701 ccggcggcgc tccacgtcca cccggatctc cccggtctgg cccgccgccc actgcgcgga
     2761 ctcctggctg gccgcgcgat agaaccagcg gatcggcgtg ttatccagta ccgtggtttt
     2821 cacaaaatca gcgggcagat cctgcccgca ctgaccgaag tcgcccagcg actggtgcgc
     2881 gagcagcagg ttgcagtccc ggctgcgcag cgtccccagc gcgttcagca cgtacttcga
     2941 cagcagatat ttaatctcat cgagcatgat gctcgcgtgc ggccacctcc ggaactcatc
     3001 ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatc ttctgcaccc ggatcaccgc
     3061 ctcatcgtcc atcgagccga tgtcgtacag gcagccgccg ccgttgatta tccccgccac
     3121 atcgataccg ctgtccgtct gcaggacgct caggtcggcc acgttctcca gcttggtgat
     3181 cagccccttc acgtcctttt tcagggcctc cggcaacgcg tacgccgccg ccagcacctg
     3241 ctgaatgttt gccccctgcg ggaactgctc cgcaatcagc ttcgccgctt tctgctcgct
     3301 gatgcggtag tggtccgcca cgtcgccggt ctcactcagg ttaaacccgg caaccagcag
     3361 attcttcagg gcgtactggt cgagatcgcg gaacagattc agctgcgccg gtttccccgg
     3421 ccgcagatcc agcagggtga agtccgggca gtgcgcccgg aagacgtgcg gcgcccacgc
     3481 gtcccctttg gggtcgaagt acaccacagc gtccccgaaa tcgcgtacgc agcgaatgag
     3541 cgcgttggtg gccatcgcgc tcttgccgct cccgggcagg ccgaggatct gaatattggt
     3601 cttgcgccag gtatcacgag ggaccagcac cggctggccc tgctcatcct tacccagaaa
     3661 caccgcctgc tgtttccggg cctgctccgt ggaaaaatcg gccagcgcgt taaccggtac
     3721 cggctccccg aaatgcaggt ggcggacgtc tgtcatttct ccggtccgct ggctgctgcg
     3781 ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca gcggccgcaa aatgaccatc
     3841 aagcagcagg aacgccagca gccgcgccag aaggacgccg gcgacaagcc ctccggcaca
     3901 ccaggctatc ccgaacaggt tagccggctg cagcaggtaa taatctgcgt tcgcatagag
     3961 gtccgggcgc aggacggttt ctccccagct ctcgataaac acggcgacaa ccagcccccc
     4021 cagaagggcc agaccggcag cgccggtcag ccagtacgcg tagttccaga catgtcgccg
     4081 tgccctgacc atccgcccgg gtgaatccag cgaggccagc accgaaaatg gcacagtccg
     4141 gttattatcg cgcttatagc gatcggcata ctcccgccgc gccttatggc tcagccccac
     4201 aaaacgcatt cggccggccc agagttcagc cagaccgaaa aacaggccga taaccagaaa
     4261 aaaaccggcc agactgtaat actcccggta cagccagctg acccacgggt aaatctgccg
     4321 ggccagctcc gggatatgcc actgccaggc aagcgaatcc gtattaaaca tgcaatctct
     4381 ctccttcaga cgacgggttt accggcgtga tgcgcagccc ccgctgcaca agccgcggac
     4441 atgccccaag gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag ctttcaccgc
     4501 ggaggccagc ctctttccgc cccatgcgga aaaggcgcag ccccggagga acaacgagca
     4561 cgccccagcg tgtgagagga aaaggcggca ccccagtgcg gtcttttccg gtgttccggc
     4621 ccccgtcaga gttcgccata gcgagcggct gaccacccgc aaaatcagga gcagctttgc
     4681 tgcttcgtca ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac
     4741 gggggccggg tgaatttctg gcgtggccgg cgtccagccg gacagagcca gaaagagggg
     4801 aaaagaccgt gcgtcggtgg cgtccagcca gcgtaagcaa ggtcttttcc cctgaagtca
     4861 gccacgcccc agcggggatg acttgcattt ctccgcaagg agaaaaaagc aacagagata
     4921 ggttaacgta aaatgttcag cgaaaaaccg acatggttca cctatcctga taattgatcg
     4981 tcagggaata gaaagactta atcaggggac aatatcccac atcagcgcgt ccggtgaccg
     5041 tcttgcaggt gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg ctccgggcgt
     5101 gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg tggataagca
     5161 ggggactaac atgtgaggca aaacggcagg gccgcgccgg tggcgttttt ccataggctc
     5221 cgccctcctg ccagagttca cataaacaga cgctttttcg gtgcatctgt gggagccgtg
     5281 aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa gatccccacc
     5341 gtttccggta ggtcgctccc tcttgcgctc tcctgttccg accctgccgt ttaccggata
     5401 cctgtccgcc gttctccctt gcgggaagtg tggcgctttc tcatagctca cacactggta
     5461 tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca
     5521 gcccgactgc tgcgccttat ccggtaactg ttcacttgag tccaacccgg gaaagcacgc
     5581 aaaacgccac tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa
     5641 acacgcggtt gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg
     5701 ttgctgtgct ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct
     5761 tcgatcaaac cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga
     5821 aaaaaaggat ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt
     5881 cagaggagga cgatggtgcc accgttccgc cctgaacatc ccggatataa tttgcctgac
     5941 atgcggccag aagaaagcca gcaacaatca atataaaaag aaaaatagtc tttacctttt
     6001 tcatatactt tccctgtatt taaagactcc ggcattcatc cctgaacacc ggaagtcagt
     6061 atttcaaatc atccttgata attttttgtt accagttacc gtcccgataa tcaaaagcta
     6121 tataacaatt atccacatca ggttttattt gatttttaaa aaatggctgt agtaaaggaa
     6181 cccattcttt cttcaaatca aactcaccat tattaatatt atcctttaaa ggcattccca
     6241 agctttcaat gacagaacca tcgtcaccaa agtctttaga atactcttcc cctttaaact
     6301 cttcagtttt cttatcaaac catgtcaaac gtaatttaag ccccatattt acctctcaaa
     6361 gatatttttt aatgttccgt tttggatctg gccctttaac ctgcttaccc gttttgggat
     6421 caaatgcgcc taggtgttcg ccatcactgg cacgataccc ttcaagctca ccgtgctggg
     6481 aatcccattc ataaatttta cgttttttat cgccatacca tcgggcccgc ttaccaccac
     6541 caccttgttt aggtgtttta ggatcacctc gctttaactc tccaagtccc tttatgtctt
     6601 ctgttttagg tactggatga taatcatggc cgtagtcttt agtccccttg cgaggcttgt
     6661 tcttttcctc atttaactta ttttctgcat ccttcgcttt tttctccttt tgtttccggc
     6721 tctccatcgc cccggtaagt gcagccttat ctctggataa ggattcattc attgcgttta
     6781 aagatgcctg agccgctttt aatttctcat tctgattctg aatatcctta tcaagatagc
     6841 gaacctgatc ctgccacccg gagccatatt catgaggcgg gttataagtc acaaaatcat
     6901 ctttatttgc ctctgcctct tttacctttt tatcggcttc ctgaacagca gcccttgcac
     6961 cggggagcgc cttctctgtc gatgcaatgc ggctgtttaa ggtcgcaata tttttatctt
     7021 cggcatccag ctcagctttc gctttatcat agtctctttt cagcccctct tccggatgag
     7081 cggcgtccca tgcctgctgg cgacgctttt cttcctcctg acgctgtttt acctgtgccg
     7141 gggtaagaac atctgtcacc gacacataaa ccggcttctg tccggtctct ttcgggaaac
     7201 gaataacggc ctcacgggag ttaccaccgg ctgtaaaacc agccggacgt gaatcacctt
     7261 tttcagcaac aatgcctttt ggcggggctt tcgctgtcgg aacacctttc ggtacgctca
     7321 cctgcagaga cgggagaccc ggaatcgaca cactgaatac ccccggacgt tttgtcggtt
     7381 tcgcatccac cacagggaca ctcattggcc gacctgcgac aaccgcaata tgctgccgct
     7441 catccttcac cacatccaca acccgttgtc tgacacgaac cgtcgcctgg tccagtggta
     7501 aagtacttgc cggagtctcc gtcaccgcat cggccggtaa tgacgtcata atttttgaca
     7561 tcattttcgg atcatctttt gcaatctcag aaggcagcac accgtagatc gcaatccccc
     7621 acagaccaaa cttaaacggc cccttcaggg cagccagcac atcagcaacg gcggccgaca
     7681 acgcatcgcc ggaaactgat aaagccagtc cttcagcccc cggagtagcc agggcaggca
     7741 gaccgaaggc catcgcagtg gccgaagact gtccgccacc agagctaccg ctatgattgc
     7801 cgctgttacc accattacca ccgggtttac tcccgccgtt accaaactcc cccggattat
     7861 agttatgaat atctccattc ggcgtatgcg tcgtacccca gcccggcccg ctgtttggat
     7921 ccgtaccacc acctgatgaa gggccacctt taccagacgt accgttcaca ttcccactgg
     7981 cctgaccacc attatgtccc agacctgaat tacccggacc tcgaccgtct ccaccactca
     8041 taccgttttc ctctttgaca tttaaaacaa taaattaaag ccaacatact gtacatatac
     8101 acagtataat tattacctta ctcctcccgt tttcaggtgt caatatatga gattagttct
     8161 catcttcagg cacaaaaaag cgcccggagc cacctgtaag cagatcgcgt cttttcccga
     8221 aaatttctca tggcaacaaa ttttattatt taagacattc atcacactta catttcagat
     8281 ggtatagaca catctgaaag ctaactggca tgaggagagg acctgcggtt aaactgttcg
     8341 ggaaacctga gacgcccgga ggccactatg acgcacctga aactgactcc cgctgaagaa
     8401 aagcgcctgg cggacgcctt tgtcgctcag gcactggcca tgggcctgaa agggaacaaa
     8461 acgctgccaa aggcggtaaa acaacaggtg cgggaaaggg tgcttcgtgc cattcaggac
     8521 gaacgtgaag ccaggcgggc gcaggaaatg cgtcgggaga aatacgaggc tacggaaacc
     8581 accttcacct ggcagccaca gcgccgccgg taaggggtat ttaccataac gtcgcattgc
     8641 ccgcaccgct cagacggtct gagggcgttt ccggcggcta cgggataaaa caggcaacaa
     8701 accacccgga aaaccgccgg aaacgcgccc ggtaaattgt aacccgctgc atgcctatgc
     8761 acgcaactgc atggcgggcc gtttggtgta aaaacgatac gataagccgt gtttatgtaa
     8821 atgaacgtgg ccgtaaagag tgacaaatat acatgtaaca ttttttactg acaggtcatc
     8881 aacccgatgc gtttaatacc ctttatcctg ctggcattgt gcagcccctt actcctgacg
     8941 gcctgtaagc attccaccac ccagccgccg aaaatttata ctctggataa gtcatctaaa
     9001 atcagtagct acgctgcggc catcaagacc gctgttgaaa tgcagatgtt tgaaccagag
     9061 gcctatgcgg gaaaaatgtg caccgtgcgg atatccatac aacgtgatgg cactctgaac
     9121 agcgccattg ctgaaggagg agatccgaaa ctctgtaaag cagcaatatc agccgttacc
     9181 cacgccaaaa ttccgccggc accggatgaa aatacctggc aaatattcaa aaacgcccct
     9241 ctggatttca gaccatgaaa gcttttactc tgacagtgct ggcggccatg ttcctcactc
     9301 cggtacatgc gataccacta aaacccgtaa ccacactgca gccaacctac ccggtacagg
     9361 ccgcagcaaa acgcatcgaa ggtcaggtga ccgccacgtt tgatattctg gacgatggac
     9421 gagtggacaa cgtgtcgctt accggctgtc accttttttt tcgggaaacg ctgacggcaa
     9481 tgaggcactg gctatatccc gcagggcacc cggcaatgcg acaggttata aattttgaat
     9541 tcagtctgaa aaatgacggc ttacaggatc cgcgaccagt caccacccaa aagctcaatg
     9601 atgatggttg ccgccagtaa caggtccttt aacccgcagg ctgcaatagt cctggatgat
     9661 gagtgagcct gtagcagccc tgattagtgg aagcgaagct gaaaaatggg cagcaaaagc
     9721 cgtatttctg gcaaatagcg ccgccacgga ttaaagtagt catgtgtaat acatatgact
     9781 acaaaggggc ttttatgtca tcagcaacat ccatcaggct cgatgaagaa cttaaggacc
     9841 ggctgaaaac gctggccgat gaccggcacc gctcagcgca cgcgctaatg ctggaggcaa
     9901 tcaccgaata catcgaccgg gaagagaagc gcagccagta cctgcgggac ggacaggccg
     9961 catggcagca ctatcaggaa acgggtctgc accttactgc ggaagaagcg gaagcctgga
    10021 taagtacctg gggaactgag aacgagcagg atgctccgcc
//
