LOCUS       NZ_CP101559             3043 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KP72 plasmid p72_5,
            complete sequence.
ACCESSION   NZ_CP101559
VERSION     NZ_CP101559.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19931468
            Assembly: GCF_024396895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wang,P.
  TITLE     Retrospective study on epidemiological characteristics of KPC-2 and
            NDM-1 producing Klebsiella pneumoniae in Kunming, Yunnan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2022) Department of Key Laboratory, Second
            Affliated Hospital of Kunming Medical University, Dianmian road No.
            374, Kunming, Yunnan 650101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUL-2022
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 250x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 06:05:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,917
            CDSs (total)                      :: 5,793
            Genes (coding)                    :: 5,650
            CDSs (with protein)               :: 5,650
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 57 of 143
            Pseudo Genes (incomplete)         :: 86 of 143
            Pseudo Genes (internal stop)      :: 21 of 143
            Pseudo Genes (multiple problems)  :: 18 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101559.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP72"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p72_5"
                     /geo_loc_name="China:Kunming"
                     /lat_lon="25.05 N 102.68 E"
                     /collection_date="2013-03"
                     /collected_by="Hu Ying"
     gene            1..282
                     /locus_tag="NMY33_RS29575"
                     /old_locus_tag="NMY33_29560"
     CDS             1..282
                     /locus_tag="NMY33_RS29575"
                     /old_locus_tag="NMY33_29560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048265035.1"
                     /translation="MMEKQQCNIFDVVESFDVFDVVEVTMEESESTYTQDNRVVVERIL
                     TIKAEKTSRGDNKSKAITMSVLSGVVVSFVGLLADIPSAVDFLKHLLL"
                     /name="hypothetical protein"
     gene            351..563
                     /locus_tag="NMY33_RS29580"
                     /old_locus_tag="NMY33_29565"
     CDS             351..563
                     /locus_tag="NMY33_RS29580"
                     /old_locus_tag="NMY33_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016054554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048265034.1"
                     /translation="MATVKRLQIDISEQSFERLKRLKESSDASSYAEVTNKAYRVYEFF
                     VDAINEGKEIKLIDKDGNETTVEFL"
                     /name="hypothetical protein"
     gene            complement(1420..1842)
                     /locus_tag="NMY33_RS29585"
                     /old_locus_tag="NMY33_29570"
     CDS             complement(1420..1842)
                     /locus_tag="NMY33_RS29585"
                     /old_locus_tag="NMY33_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_108967721.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLGGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1842..2033)
                     /locus_tag="NMY33_RS29590"
                     /old_locus_tag="NMY33_29575"
     CDS             complement(1842..2033)
                     /locus_tag="NMY33_RS29590"
                     /old_locus_tag="NMY33_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_048265037.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDDN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2082..2562
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.278"
                     /coverage="92.0"
                     /evalue="1.27e-168"
ORIGIN
        1 atgatggaaa agcagcaatg taatatcttt gatgttgttg aaagcttcga tgtttttgat
       61 gttgtcgaag tgacaatgga agaaagcgag tctacctaca cccaagataa tcgtgtagtg
      121 gtggaaagaa tactgacaat caaagcggag aaaacaagcc gcggagataa caaaagcaaa
      181 gcaataacaa tgtccgtgtt aagcggtgtt gttgtaagtt ttgtagggtt acttgctgat
      241 attccaagtg cagtggattt tttaaaacat ttgctgcttt agttctaagg cgccggagct
      301 cctccggcaa gccttatcgc acatgcattt aagtatgtgc aggagtaatt atggcgacag
      361 taaaaagatt gcagatagat atatcagagc aatcctttga aagacttaag agactaaaag
      421 agagctctga tgcttcctca tatgctgaag taacaaacaa ggcatatagg gtctatgaat
      481 tctttgttga tgctatcaat gaaggaaaag agataaaact catagacaaa gatgggaatg
      541 aaacgaccgt agagttttta tagaaaatta ttaactacac gattataccc ctgccatgca
      601 ggggtttttt tgtatagaaa tgatcacatc tataattcaa taaaacgaat catggttgca
      661 tttaccgtta cgcattgatc cagatttacc ataacgcgtc attacgcgca ccgctcaaac
      721 gccccagaaa cgattctggg ccgtaaccca tcaaatccta catcttaccc cctgaaaacg
      781 cctcaaaatc gccccagggc tttttacggg catgaattgt tatgcaccag actgactggt
      841 ggcggatttc agcctgaatt cacgccagac aactcaaaac ccgcaccaaa cccgactgaa
      901 aacggtgaac ggggacgcaa taaattgcgt ggccgtgaag cgttgccggc gcagccggaa
      961 aaccggtttt cagcacgttc aggaatcggg ttcgtcgacg gccggcaatc acgttatggc
     1021 aagtcgccgg acgactcacc acacgattca ccggcgattc gactcgtgac ttgactctag
     1081 agtcaccatg gtcaaaaagc caccaacccg gcaagtcgac tctagagtca agtcatcata
     1141 acgtactgat tatatggcgt gattcacata tgagtcgtgg gcaagtcatc ggcgattcgc
     1201 catagagtcg tccacgactc ggattcacct tacaaaaaat aaaactgcgt aatttaaaat
     1261 agataactga ttgaaaataa aggtctatct gcaaaatttt aattttgcgt agtgtgtggg
     1321 tatttttagt ggtgaggagc gttcagggat ctgacgattt ttgggaaggt taaggaactc
     1381 cccggaatgg ccggagagca aaatggaagc atggcgggat caggattttt tccagaattt
     1441 ccaccacggg gaatggggtg gtgaagaagg ttcgtgcttg tgctccagaa gctgcatagc
     1501 ctgacgaagg gaatcaatat gctcatctcg agccttaaca gcccctttaa gaccatcgtt
     1561 ttcagcttca agtaaagcga ttctatgttg taaatcatgt aaaccaacgc tgttaatacc
     1621 tgtagattct gcgtcacttt ttacatcata ttttacagta tcgattacac caccaagaga
     1681 accaaacact ctaattagct ctgaagtatc aataacttta atagctttcc cgtgctttac
     1741 cacagaggtg taacttaact tcccactgtt aatcatcttg taaattgtgg ggcgactaac
     1801 cttaacaagc cgtgccgctt cactaataga cactctggcc atcagttatc atcctgtaat
     1861 tcatccagtc tcgaggacag tgtacggaaa agatgctccg catcatcatg gagtttctca
     1921 caaaggtcgg cctcgacatc gagatcaagc tcgttaagct tctccagcag gagcagagac
     1981 tgatgctgga tgaatttagc catgttcagc gctgttttct gttgtttcgt cataagaagc
     2041 ctcagagcgt cagtgctcag tgtgaaactg atatcttttc cgcttcctcg ctcactcagt
     2101 cgctacgctc ctgccgttcg gctgcggcga gcggtagaga ctcacgtata agggcataga
     2161 gcgacagcgc agagagccag agaaaacaaa agcaggccac agagttgccg tttttccata
     2221 ggctccgccc ccctgacaag catcacagaa acagacgctc aaatcagtgg tggcgaaacc
     2281 cgacaggact ttaaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg
     2341 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca
     2401 cgccagacac tcggttccgg gaaggcagtt cgctccaagc tggactgtat gcacgaaccc
     2461 cccgttcagt ccgactgctg cgcctgttcc ggtaactatc gacttgagtc caaaccggaa
     2521 agacacgaca aagcgccact ggcagcagcc actggtaact gatttagagg agttagtctt
     2581 gaagttatgc gccggttaag gctaaactga aagaacaagt tctggtgact gcgctcctct
     2641 taagccagtt acccggtctt caaagccgca aactttgtag accttaaaaa aaccgccccg
     2701 caaaggcggt tttttttatg cctaaagact accccgcagc agaatgcgcc gtcaacaacc
     2761 attcaaaaca atgcacttag tattacaaca accaatacaa aagacattaa aatttgacta
     2821 caaaaaaaac tagtgttagc attaccatca tgagcacaaa atcgagtgcc aaccgaattc
     2881 acagtagtag atatatgtaa tcatgcataa atgttgattt gttaaataaa tgttaaacta
     2941 taggaattat aaaggatgaa aatgcagata aatttacaga ttttgggtat acccaaaaac
     3001 cagcgccacc atagatatag gtggttaact acaaggcaaa gta
//
