LOCUS       NZ_CP101553             2469 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KP82 plasmid p82_7,
            complete sequence.
ACCESSION   NZ_CP101553
VERSION     NZ_CP101553.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19931475
            Assembly: GCF_024396875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Wang,P.
  TITLE     Retrospective study on epidemiological characteristics of KPC-2 and
            NDM-1 producing Klebsiella pneumoniae in Kunming, Yunnan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Wang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2022) Department of Key Laboratory, Second
            Affliated Hospital of Kunming Medical University, Dianmian road No.
            374, Kunming, Yunnan 650101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUL-2022
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 230x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 06:15:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,821
            CDSs (total)                      :: 5,697
            Genes (coding)                    :: 5,552
            CDSs (with protein)               :: 5,552
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 51 of 145
            Pseudo Genes (incomplete)         :: 99 of 145
            Pseudo Genes (internal stop)      :: 17 of 145
            Pseudo Genes (multiple problems)  :: 19 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101553.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP82"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p82_7"
                     /geo_loc_name="China:Kunming"
                     /lat_lon="25.05 N 102.68 E"
                     /collection_date="2013-05"
                     /collected_by="Hu Ying"
     gene            1..504
                     /locus_tag="NMY34_RS29085"
                     /old_locus_tag="NMY34_29065"
     CDS             1..504
                     /locus_tag="NMY34_RS29085"
                     /old_locus_tag="NMY34_29065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152929193.1"
                     /translation="MKIAAALGVCHFLVYKTTTPECWRRRLRMILAKKRPFPGKGHARM
                     TLWVQRLHINYKIASAFTPERGCTTELHQKYIKITSLLRQIAGVQQNYIKSTSLLRHFY
                     IIFTSDLTSELFVHPPPLLGPPARAENGFSGCAGNASRPRNKLRIRSPLSGWFVVAFRA
                     FIGF"
                     /name="hypothetical protein"
     gene            complement(738..1163)
                     /locus_tag="NMY34_RS29090"
                     /old_locus_tag="NMY34_29070"
     CDS             complement(738..1163)
                     /locus_tag="NMY34_RS29090"
                     /old_locus_tag="NMY34_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="WP_004884531.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGFIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMNSKQQ"
                     /name="PH domain-containing protein"
     gene            2141..2332
                     /locus_tag="NMY34_RS29095"
                     /old_locus_tag="NMY34_29075"
     CDS             2141..2332
                     /locus_tag="NMY34_RS29095"
                     /old_locus_tag="NMY34_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(1605..1959)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.348"
                     /coverage="82.0"
                     /evalue="2.93e-164"
ORIGIN
        1 atgaaaatcg cagctgcgtt aggtgtatgt cattttttag tatataaaac gaccactccc
       61 gagtgctggc gccgcaggtt aaggatgatt ctggctaaaa agcgtccttt tccgggaaag
      121 ggtcatgctc gtatgaccct atgggttcag cgactacaca taaactacaa aattgcatct
      181 gcttttaccc cagaacgcgg gtgtacaaca gaattacatc aaaagtacat caaaattaca
      241 tcacttttac gtcagatcgc gggtgtacaa cagaattaca tcaaaagtac atcactttta
      301 cgtcactttt acatcatttt tacgtcagat ctgacctctg aactattcgt acatccgccc
      361 cctcttcttg ggcctcctgc acgtgctgaa aatggttttt ccggctgtgc cggcaacgcc
      421 tcacggccac gcaataaatt gcgcatccgt tcaccgttat cggggtggtt cgtggtggct
      481 ttcagggcgt tcatcggatt ttagccgcta aaatcccggc caggtatgtc cgtgcatagc
      541 catgcagtcg cgccagaatc atcaggggcg ttttttctcg atatgggcac agaaaggtgt
      601 gtgtttcagg ccggaggcct cagaaacgcc ctgagggcct ctgaggcggt gcgggcaacg
      661 gatgttatgg taaatccagc tgattggcag gtacaaaaaa agccccgcag ggcttttttt
      721 gtaatgctac gaggaagcta ctgctgctta gaattcattt cctcgatatg attgttcaca
      781 atcgatctga attgcattgg cgccttgatg tttggaattg gagtgttggt tccgccagtg
      841 cctttaatca cgatagaccc atagttaaag attcgtccca gtatcccttg atgtatgcca
      901 aggctttcaa ctttatcaat acggagttcc accgtattgc gtcggataaa acccactttt
      961 gcgatgactc tttggttagt taatgcaagc tccgttgacc aaactctaag gagcacaggg
     1021 attattataa gtaaccctaa gccgtaaaaa ggcagcaaca agattccgat aattactggg
     1081 atgattgttg gaatccagga ggtttgagcg cgagatatta cggattcatt tctcgttaat
     1141 gattggtcta cgtagcttcc cataaggtta tatccattta gaaaaaatag gatttttccc
     1201 tttttaaggc gaatcagcaa tagaaacttt atgtctccgt ggtgggtata caaaatttac
     1261 ggagagcgta gcgaccgtcg cacagttcgg ttctttcctt tcgtgacgca actggcttga
     1321 ttctgtggtg gtggtggctg ctgctatgtc tcacatactc ttggcgttgc aatcctttat
     1381 cccatatctg tcgaatatca cagatgatct tcttgagatc ctgtccgttg cgtcgtaatc
     1441 tctttctctg gaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt tctcagagct
     1501 accaactctt tgaaccgcgg taactggctt ggaggagcgc agtaaccaaa tctgtccttt
     1561 cagtttagcc ttaaccggcg cataacttca agactaactc ctctaaatca gttaccagtg
     1621 gctgctgcca gtggcgcttt gtcgtgtctt tccgggttgg actcaagatg atagttaccg
     1681 gacaaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga
     1741 actgcctacc cggaactgag tgtcaggcgt ggaatgagat aaacgcggcc ataacagcgg
     1801 aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc taccaggggg
     1861 aaacgcctgg gatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat
     1921 tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cgccccggcg gggcgtggcc
     1981 ttttcgccct tgtccttagc tttcgttatg cttaccgtta cgttatctgc ttatcctccc
     2041 tccgctcgcc gcagactcat gaccgaacgg agtgagcgaa tgagcgagga agcggaaaac
     2101 catccgaacg tacagtaagc actttcgcgc cggggctttc atgaacaaac aacaaaagac
     2161 tgccgttaac atggctaaat tcatccagga tcagtccctg cttcttctgg acaggcttaa
     2221 tgagctggat ctggatcatg aggctgattt ctgcgagaaa ctccatgagg atgcggaacg
     2281 cctttaccga agcctgtcag cgcgcctgaa tgagcagcag gacggtgcgt aatggctccg
     2341 ttctgggtat taccggagta ataccggcat cgccttaatt ttcattgtgt tagcgcttaa
     2401 cattattacc ccggtaatac ccagaatccc tgtgtttttt cttttttatt tatttttcat
     2461 gaagttgcg
//
