LOCUS       NZ_CP101552             2936 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KP82 plasmid p82_6,
            complete sequence.
ACCESSION   NZ_CP101552
VERSION     NZ_CP101552.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19931475
            Assembly: GCF_024396875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Wang,P.
  TITLE     Retrospective study on epidemiological characteristics of KPC-2 and
            NDM-1 producing Klebsiella pneumoniae in Kunming, Yunnan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Wang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2022) Department of Key Laboratory, Second
            Affliated Hospital of Kunming Medical University, Dianmian road No.
            374, Kunming, Yunnan 650101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUL-2022
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 230x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 06:15:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,821
            CDSs (total)                      :: 5,697
            Genes (coding)                    :: 5,552
            CDSs (with protein)               :: 5,552
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 51 of 145
            Pseudo Genes (incomplete)         :: 99 of 145
            Pseudo Genes (internal stop)      :: 17 of 145
            Pseudo Genes (multiple problems)  :: 19 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101552.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP82"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p82_6"
                     /geo_loc_name="China:Kunming"
                     /lat_lon="25.05 N 102.68 E"
                     /collection_date="2013-05"
                     /collected_by="Hu Ying"
     gene            13..360
                     /locus_tag="NMY34_RS29065"
                     /old_locus_tag="NMY34_29045"
     CDS             13..360
                     /locus_tag="NMY34_RS29065"
                     /old_locus_tag="NMY34_29045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110958373.1"
                     /translation="MIMILWWISSFITHMMKVGNVREYGPTKSHLLLMVTTLPAGVGVF
                     IYPIVEVIWGDLSLLKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIA
                     GLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(1086..1508)
                     /locus_tag="NMY34_RS29070"
                     /old_locus_tag="NMY34_29050"
     CDS             complement(1086..1508)
                     /locus_tag="NMY34_RS29070"
                     /old_locus_tag="NMY34_29050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004207907.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAISTEVNTIGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHDPSSPAHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1508..1699)
                     /locus_tag="NMY34_RS29075"
                     /old_locus_tag="NMY34_29055"
     CDS             complement(1508..1699)
                     /locus_tag="NMY34_RS29075"
                     /old_locus_tag="NMY34_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004207910.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2557..2934
                     /locus_tag="NMY34_RS29080"
                     /old_locus_tag="NMY34_29060"
     CDS             2557..2934
                     /locus_tag="NMY34_RS29080"
                     /old_locus_tag="NMY34_29060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110982627.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     Typing          1867..2232
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 atgaatttcc ttatgatcat gatcctatgg tggatatcgt cattcatcac acacatgatg
       61 aaagttggaa atgtcagaga gtacggtccc actaaaagcc atttactgtt gatggttact
      121 acgcttcctg ctggcgttgg tgtttttatc tatccgatag tagaggtgat atggggagat
      181 ctgtcgttac tgaaagttgc tggttatacc tttttatccc tcattgttgg cggacttctt
      241 gccaatatca ttgttcgcgg attatctgcg acaacattcg gcattttgct cgtaaatcca
      301 ttagcaatga tatgtagcat tgccggactg atagtccttg ctatccaaca tttaccataa
      361 catccgttat cagcaccgcc tgagaggccc tcagcgcgtt tctgacggga aaccggtaaa
      421 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
      481 catgagtatg cgcgcaagtg catagcgcgg aaattcacac cattcagccg gggtaagtcc
      541 tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
      601 gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc acgttcagga atcgggttcg
      661 tcgacggccg gcaatcacgt tatggcaagt caccggacga cttagcacac gattcaccgg
      721 cgattcgaat cgtgaattga atcaagagtc accatgaaca aaaaacaccc aaagtgatga
      781 attgaatcaa gagtcgattc atcataacaa tatgataata aagcgtgatt cacatatgaa
      841 tcgtgggcaa ttcgtcagcg attcgccata gagtcgtcca cgacttggat ctgcactttg
      901 taacacatca ccgcacattt atttttcatt aaaccattgt tgtaaataag gaatctgcaa
      961 aattttaatt ttgcgtgggg tgtgggtatt tttagtggtg aggagcgttc agagatctgg
     1021 cgatttttgg gaaggttcag gaactccccg ggatcgccgg agagcaaaac tgaaacatgg
     1081 caggatcagg attttttcca gaatttccac cacggagaat gagccggtga agaggggtcg
     1141 tgcttgtgct ccaaaagctg catagcctgg cgaagggaat caatatgctc atcgcgtgct
     1201 ttaacagccc ctttaagacc atcgttttca gcttcaagta aagcgattct atgttgtaaa
     1261 tcatgtaaac caatggtgtt aacctctgta gaaattgcgt cactttttac atcatatttt
     1321 acagtatcag ttacaccatc aagagaacca aacaccctaa taagctcgga tacatcaata
     1381 actttaatat ccttcccgtg ctttacaaca gaggtataac ttaacttccc gctgttaatc
     1441 atcttgtaaa tagtgggacg actaacctta acaagccgtg cagcttcact aatagacact
     1501 ctggccatca gttaccatcc tgtaatgaat ccagtctcga ggagagtgta cggaaaagat
     1561 gctctgcatc atcgtggagt ttctcacaaa ggtcggcctc agcatcaaga tcaagctcgt
     1621 taagcttctc cagcaggagc agagactgat tctggatgaa tttagccatg ttcagcgccg
     1681 ttttctgttg tttcgtcata aagggcctca gagcgtcagt gctcagtgta aaacctgtct
     1741 cttttccgct tcctcgctca ctcagtcgct acgctccggt cgttcgactg cggcgagcgg
     1801 caggagcatg catagaaaag ctcgagaaaa ccgagcggtc aaaggaggaa aaggaggaaa
     1861 aaaaggaaag gccacggcgt tgccgttttt ccataggctc cgcccccctg acaagcatcg
     1921 cagaaacgga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc
     1981 gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     2041 cactccgctg ttatggccgc gtttatctca ttccacgcct gacactcagt tccgggtagg
     2101 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct
     2161 tatccggtaa ctatcgtctt gagtccaatc cggaaagaca cgacttatcg ccactggcag
     2221 cagccactgg taacggattc gcagaacaaa gaaatgttga agtgtgaggt gaagtacagc
     2281 tacactgtaa taactaattt ggtttctgtg ctctgcgaaa gccagttacc tcggttcaaa
     2341 gagttgctgc tctaagaacc ttcgaaaaaa ccgccttgct gggcggtttt ttcgtttcca
     2401 gggcaagaga ttacgacgca acagaaagga tctcaagagg atcctattcc atccctctcc
     2461 cccccaaagg gaaaccagca ccactccacg acaccagttc tttgaagaaa attattgaac
     2521 cccttgattt taataataaa aaaaacagat ataatcatga tccacaatca cccaacttgg
     2581 gtaataaaat caatgattta taggttaggt attttatcaa tatcgctgct tacagttggt
     2641 tgtgtttcta ccagcaacgt tcttgagtta ggtagggata catatacggt ttcagcaact
     2701 gctgatggtt ttagagatgc ggcatcagca cgtcaaagtg cctttaaaac aggttctgag
     2761 aaatgtaata gcctcggtaa aaaattcctg ctcactaacg aaaacgtggc taacacaaga
     2821 atggggatag ataccacagt aacactctac ttccgttgtc ttgacgaaag agacagcgag
     2881 tatgtacgac caaatctaca gaaagcacct gatctacgca tcgagagtaa ataatt
//
