LOCUS       NZ_CP101551             4234 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KP82 plasmid p82_5,
            complete sequence.
ACCESSION   NZ_CP101551
VERSION     NZ_CP101551.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19931475
            Assembly: GCF_024396875.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Wang,P.
  TITLE     Retrospective study on epidemiological characteristics of KPC-2 and
            NDM-1 producing Klebsiella pneumoniae in Kunming, Yunnan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4234)
  AUTHORS   Wang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2022) Department of Key Laboratory, Second
            Affliated Hospital of Kunming Medical University, Dianmian road No.
            374, Kunming, Yunnan 650101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUL-2022
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 230x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 06:15:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,821
            CDSs (total)                      :: 5,697
            Genes (coding)                    :: 5,552
            CDSs (with protein)               :: 5,552
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 51 of 145
            Pseudo Genes (incomplete)         :: 99 of 145
            Pseudo Genes (internal stop)      :: 17 of 145
            Pseudo Genes (multiple problems)  :: 19 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101551.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP82"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p82_5"
                     /geo_loc_name="China:Kunming"
                     /lat_lon="25.05 N 102.68 E"
                     /collection_date="2013-05"
                     /collected_by="Hu Ying"
     gene            1..288
                     /locus_tag="NMY34_RS29035"
                     /old_locus_tag="NMY34_29015"
     CDS             1..288
                     /locus_tag="NMY34_RS29035"
                     /old_locus_tag="NMY34_29015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074184133.1"
                     /translation="MSDDSSVNLVPVTVFESQVEAVEGITIVVRAPSNTMVPEYTYVRR
                     SPGDNTVGEWMNNRINPSLNGLEVTIIDGEHESPRRSTKRLSTLRDSYER"
                     /name="hypothetical protein"
     gene            complement(427..1038)
                     /locus_tag="NMY34_RS29040"
                     /old_locus_tag="NMY34_29020"
     CDS             complement(427..1038)
                     /locus_tag="NMY34_RS29040"
                     /old_locus_tag="NMY34_29020"
                     /inference="COORDINATES: protein motif:HMM:NF025107.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_074184132.1"
                     /translation="MSTENAQKEQKKESLITTHWGEDATLHGWTAVPNSLLMLQGDLGI
                     GSTEMCILLNVLMHQWPESGESISFPSIGTIASRMGVSKRTIQRGVSNLESLGILTRHQ
                     STRNDPRTNGANIFDSTPLKEHLNKKSKGITISRNKKKERKNIGTISNIKLPRLCPKCL
                     KTKATSYEEIVSFFGIRKTMAGEVINSYCKECRASEKNIF"
                     /name="helix-turn-helix domain-containing protein"
     gene            1357..2082
                     /locus_tag="NMY34_RS29045"
                     /old_locus_tag="NMY34_29025"
     CDS             1357..2082
                     /locus_tag="NMY34_RS29045"
                     /old_locus_tag="NMY34_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_074184131.1"
                     /translation="MASMETEQQPQKNIEQIEVKAKRVQCFIDGYNLYHAIEETGNKAL
                     HWVDLKSLCKRYLPKDASIENVFWFSAKPSHLSRTVNDNYQNYTNALTECGVDVIGGRF
                     KRKNVKCEADNGCGEVFHKHEEKESDVNLAISLVSGACNNEYDIAIIITADSDLCPPIK
                     YVKERFPDKELWLVAPPGRIKRAADLCLMSSKNMEINSNALRASCMRDEFYKGGVLVAR
                     RPQEWRAIANTGQRRTRSR"
                     /name="NYN domain-containing protein"
     gene            complement(2287..2400)
                     /locus_tag="NMY34_RS29050"
                     /old_locus_tag="NMY34_29030"
     CDS             complement(2287..2400)
                     /locus_tag="NMY34_RS29050"
                     /old_locus_tag="NMY34_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074184130.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDNAPLAAATGN"
                     /name="replication initiation protein"
     gene            2815..3006
                     /locus_tag="NMY34_RS29055"
                     /old_locus_tag="NMY34_29035"
     CDS             2815..3006
                     /locus_tag="NMY34_RS29055"
                     /old_locus_tag="NMY34_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3006..3407
                     /locus_tag="NMY34_RS29060"
                     /old_locus_tag="NMY34_29040"
     CDS             3006..3407
                     /locus_tag="NMY34_RS29060"
                     /old_locus_tag="NMY34_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024623230.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2288..2695)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.195"
                     /coverage="99.0"
                     /evalue="1.29e-162"
ORIGIN
        1 atgtctgatg attccagtgt taacttagtt cctgtgacgg tttttgagtc gcaggttgaa
       61 gcggttgagg gtattaccat cgtggttaga gcaccatcga atactatggt tcctgaatat
      121 acgtatgtta gacgatcccc tggcgataac accgtaggag agtggatgaa caatagaatt
      181 aacccgtcat taaatggcct tgaagtcacg attattgatg gggagcatga atcgcctcgt
      241 cgttcaacta agcgcctatc tactttacgg gatagttacg agcgataacc gctatcacca
      301 tgaataagag aagccgctta tacagcggct tctttatgtg gtacgggcaa tgaggcgtta
      361 tggtaaatgg ggcaaagttt taattggact ctgcaccttt tcccatatat ttatatgttt
      421 taatatttaa aatatgtttt tttcagaagc tcgacattct ttgcaatatg aatttattac
      481 ttccccagcc attgtctttc ggatgccaaa aaaactaacg atctcttcgt aactggttgc
      541 tttagttttt aaacatttag ggcaaagtcg aggtaatttt atattggata tggttccgat
      601 gttctttctt tctttctttt tgtttctgct aattgttata cctttagatt ttttatttaa
      661 atgttctttt agtggagttg aatcgaatat atttgcccca ttggttctag ggtcattcct
      721 tgttgattgg tgtctggtca atattcctaa tgattctaga ttgctaactc cacgttgaat
      781 cgttctttta gatacaccca ttcttgatgc aatggtacca attgaaggaa aagatattga
      841 ctcccctgat tcaggccatt gatgcattaa tacattcagt agaatacaca tctcagtact
      901 cccaatccca agatcgcctt gcagcataag gaggctgttt ggtacagctg tccacccatg
      961 tagagttgca tcctctcccc agtgcgtagt gattaatgac tctttttttt gttctttctg
     1021 tgcgttttct gtactcatgg gtgcttttct cgtgaaaaaa aatcaaaacg tgaacagtta
     1081 ctacctaact actcttataa agagtagtta ggtagtaaca tgtatcttta taggatgaca
     1141 tatatgtcat ccatttgctc tttttttata tagaaattag tttgagttca ctacctaaga
     1201 gttctgctct tagtggctat ccaggtcgag gcttggaagc gcgttgtaca caattcttcc
     1261 tgtatcggat gcgtccatac agtggtctgt taactgcggt taagagacgc aaaaaggcct
     1321 gcatatgcag gcctttttgc tatctagagg tcaaatatgg caagtatgga aacagagcag
     1381 caaccacaga aaaatattga gcaaatcgaa gtaaaagcaa agcgagttca gtgtttcatt
     1441 gatggctata atttatatca tgctattgaa gaaacgggta ataaagctct tcattgggtt
     1501 gaccttaaat ctttatgtaa aagatacctt cctaaagatg catcgataga gaatgttttt
     1561 tggttttctg ccaagccatc acacttatcg agaacggtaa atgataacta tcaaaattac
     1621 acgaatgctt taactgaatg tggtgttgat gttatcggtg ggcgttttaa aagaaaaaat
     1681 gtaaagtgcg aagctgataa tggatgtggt gaggttttcc ataagcatga agaaaaagaa
     1741 tccgatgtta atttggctat aagcctagtg tctggtgctt gtaataatga gtatgatata
     1801 gccattatta tcactgcgga ttctgactta tgccctccta ttaaatacgt aaaagagcga
     1861 ttccctgata aagaactttg gcttgtagct ccaccggggc gaatcaagcg agcggcagat
     1921 ctctgcctta tgtcatcgaa aaatatggag atcaacagta atgctttgag agctagctgt
     1981 atgcgtgatg agttctacaa aggcggagtg cttgtagcca gaaggcctca ggaatggcga
     2041 gctattgcca ataccgggca aagaagaact cgaagtcgtt aggctttgtt gacgtagcta
     2101 gagtaaggag attaatctag ctaggttgaa acgaaaaaac cgccttggcg ggcggttttt
     2161 cgaaggttca tagagttggc gctctttgaa ccgaggtaac tggcttgggg aggagcgcag
     2221 tcaccaaaac ttgttctttc agtttagcct taataggcgc atgacttcaa gaccacctcc
     2281 tctaaatcag ttaccagtgg ctgctgccag tggcgcgttg tcgtgtcttt ccgggttgga
     2341 ctcaagtcga tagttaccgg ataaggcgca gcagtcggac tgaacggggg gttcgtgcat
     2401 acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca
     2461 aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca
     2521 cgagggagcc acctatcaga aatgataggt atctttatag tcctgtcggg tttcgccacc
     2581 actgatttga gcgtctgttt tctgtgatgc ttgtcagggg ggcggagcct atggaaaaac
     2641 ggccgctgcg cggcctttcc ttttcccatc acgcgaccgt tcggttttct cgagctttat
     2701 acgcatgctc ctgccgctcg ccgcagccga acggcaggag cgtagcgact gagtgagcga
     2761 ggaagcggaa aagagaacat ccctacattg agcactgacg ctctgaggct ccttatgacg
     2821 aaacaacaga aaacagcgct gaacatggct aaattcatcc agaatcagtc tctgctcctg
     2881 ctggagaagc ttaacgagct tgatcttgat gctgaggccg acctttgtga gaaactccac
     2941 gacgatgcgg agcatctttt ccgtacactg tcctcgagac tggatgcatt acaggatggt
     3001 aactgatggc cagagtgtct attagtgagg ctgcccgact gacgggtaag agtagaacaa
     3061 cactgcacag gttaattaaa gcaggtgacc tgtccacctg tagcggtgaa cgcaatgcaa
     3121 aaatggtaga tacttcagag cttttacgtg tctttggtcc ctttgaacaa ctgaagggtg
     3181 aacaccattc aggacaggtc tctgaacagc gtgatacagc ctctgctgac cagagtgaac
     3241 aggtgattcg tcatctgaaa caggagattg aacacctgaa caccttactt ttgtccaagg
     3301 attcgcacat tgacagcctg aaacaggcca tgctccttct tgaaaatcac caggcaaaat
     3361 taactgaatc cccggcgcca tggtggaaat tctggaaaaa atcctgaccc cgtcatgctt
     3421 cagttttcct ctccggccat cccggggagt tcctgaacct tcccaaaaat cgtcagatct
     3481 ctgaacgctc ctcaccacta aaaataccca caccccacgc aaaattaaca ttttgcggat
     3541 ttgtatttaa tatcagtagc ttaagttggt gaaatttcgt tttttaaatt tgatttatat
     3601 agatccgact ctatgacgac tcgtcataga gtccccggcg agtcgtcata gagtcggtaa
     3661 cgactcagga ctataaaaac aatgagttaa taacgacccg actctagaca cgactccccg
     3721 tttgggggtt ttttagacgt ggcgactctg ggtacgactc tagtgaagag tcgccggtga
     3781 atcgtgtggt gagtcgttcg gtgacttgcc ataacgtgat tgccggccgt cgacgaacac
     3841 gattcctgaa cgtgctgaaa accggttttc cggctgcgcc ggcaacgctt cacggccacg
     3901 caataaattg cgtccccgtt caccgttttc agtcgggttt ggtgcgggtt ttgagttgtc
     3961 tggcgtgaat tcaggatgaa atctacctcc agccagtctg gtgcataaca attcatgccc
     4021 gtaaaaccca tcagcgcgat tttgaggcgt tttcaggggg taagacgtag gatttaatgg
     4081 gttacgcccc agaatcgttt ctggagcgtt tgagcggtgc gcgtaatggg acgttatggt
     4141 aaatcgtaca ttttgaaatt ttcagtagac atttgttggg ttactcgcta acttcctgct
     4201 aactagaatt taattgcttg gtaaggtcaa tata
//
