LOCUS       NZ_CP101544            10061 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain KP21 plasmid p21_2,
            complete sequence.
ACCESSION   NZ_CP101544
VERSION     NZ_CP101544.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19931439
            Assembly: GCF_024396855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Wang,P.
  TITLE     Retrospective study on epidemiological characteristics of KPC-2 and
            NDM-1 producing Klebsiella pneumoniae in Kunming, Yunnan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Wang,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2022) Department of Key Laboratory, Second
            Affliated Hospital of Kunming Medical University, Dianmian road No.
            374, Kunming, Yunnan 650101, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 10-JUL-2022
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 250x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 06:14:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,604
            CDSs (total)                      :: 5,476
            Genes (coding)                    :: 5,342
            CDSs (with protein)               :: 5,342
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 56 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 12 of 134
            Pseudo Genes (multiple problems)  :: 14 of 134
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101544.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP21"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p21_2"
                     /geo_loc_name="China:Kunming"
                     /lat_lon="25.05 N 102.68 E"
                     /collection_date="2013-04"
                     /collected_by="Hu Ying"
     gene            1..258
                     /locus_tag="NMY29_RS27910"
                     /old_locus_tag="NMY29_27890"
     CDS             1..258
                     /locus_tag="NMY29_RS27910"
                     /old_locus_tag="NMY29_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            343..492
                     /locus_tag="NMY29_RS27915"
                     /old_locus_tag="NMY29_27895"
     CDS             343..492
                     /locus_tag="NMY29_RS27915"
                     /old_locus_tag="NMY29_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1976..3940
                     /locus_tag="NMY29_RS27920"
                     /old_locus_tag="NMY29_27900"
     CDS             1976..3940
                     /locus_tag="NMY29_RS27920"
                     /old_locus_tag="NMY29_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3940..4671
                     /gene="mobC"
                     /locus_tag="NMY29_RS27925"
                     /old_locus_tag="NMY29_27905"
     CDS             3940..4671
                     /gene="mobC"
                     /locus_tag="NMY29_RS27925"
                     /old_locus_tag="NMY29_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(5236..5415)
                     /locus_tag="NMY29_RS27930"
                     /old_locus_tag="NMY29_27910"
     CDS             complement(5236..5415)
                     /locus_tag="NMY29_RS27930"
                     /old_locus_tag="NMY29_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5441..5869)
                     /locus_tag="NMY29_RS27935"
                     /old_locus_tag="NMY29_27915"
     CDS             complement(5441..5869)
                     /locus_tag="NMY29_RS27935"
                     /old_locus_tag="NMY29_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6033..6347)
                     /locus_tag="NMY29_RS27940"
                     /old_locus_tag="NMY29_27920"
     CDS             complement(6033..6347)
                     /locus_tag="NMY29_RS27940"
                     /old_locus_tag="NMY29_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6335..6613)
                     /locus_tag="NMY29_RS27945"
                     /old_locus_tag="NMY29_27925"
     CDS             complement(6335..6613)
                     /locus_tag="NMY29_RS27945"
                     /old_locus_tag="NMY29_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6788..7153)
                     /locus_tag="NMY29_RS27950"
                     /old_locus_tag="NMY29_27930"
     CDS             complement(6788..7153)
                     /locus_tag="NMY29_RS27950"
                     /old_locus_tag="NMY29_27930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7150..7521)
                     /gene="tolA"
                     /locus_tag="NMY29_RS27955"
                     /old_locus_tag="NMY29_27935"
     CDS             complement(7150..7521)
                     /gene="tolA"
                     /locus_tag="NMY29_RS27955"
                     /old_locus_tag="NMY29_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7795..8040)
                     /locus_tag="NMY29_RS27960"
                     /old_locus_tag="NMY29_27940"
     CDS             complement(7795..8040)
                     /locus_tag="NMY29_RS27960"
                     /old_locus_tag="NMY29_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8367..10052
                     /locus_tag="NMY29_RS27965"
                     /old_locus_tag="NMY29_27945"
     CDS             8367..10052
                     /locus_tag="NMY29_RS27965"
                     /old_locus_tag="NMY29_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3367..10061
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(596..1174)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3941..4668
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atggggctta aattacgttt gacatggttt gataagaaaa ctgaagagtt taaaggggaa
       61 gagtattcta aagactttgg tgacgatggt tctgtcattg aaagcttggg aatgccttta
      121 aaggataata ttaataatgg tgagtttgat ttgaagaaag aatgggttcc tttactacag
      181 ccatttttta aaaatcaaat aaaacctgat gtggataatt gttatatagc ttttgattat
      241 cgggacggta actggtaaca aaaaattatc aaggatgatt tgaaatactg acttccggtg
      301 ttcagggatg aatgccggag tctttaaata cagggaaagt atatgaaaaa ggtaaagact
      361 atttttcttt ttatattgat tgttgctggc tttcttctgg ccgcatgtca ggcaaattat
      421 atccgggatg ttcagggcgg aacggtggca ccatcgtcct cctctgaact gacggggatc
      481 gcggttcagt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      541 tttgccctgt aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt
      601 ggggaactgc ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt
      661 ccagtgtagc cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat
      721 cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gctttcccgg
      781 gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggagttc
      841 ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc agtgtgtgag
      901 ctatgagaaa gcgccacact tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg
      961 cagggtcgga acaggagagc gcaagaggga gcgacctacc ggaaacggtg gggatcttta
     1021 agtcctgtcg ggtttcgccc gtactgtcag attcatggtt gagcctcacg gctcccacag
     1081 atgcaccgaa aaagcgtctg tttatgtgaa ctctggcagg agggcggagc ctatggaaaa
     1141 acgccaccgg cgcggccctg ccgttttgcc tcacatgtta gtcccctgct tatccacgga
     1201 atctgtgggt aactttgtat gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta
     1261 gcgaccgagt gagcgaggag gcgcaatttc cccggcacct gcaagacggt caccggacgc
     1321 gctgatgtgg gatattgtcc cctgattaag tctttctatt ccctgacgat caattatcag
     1381 gataggtgaa ccatgtcggt ttttcgctga acattttacg ttaacctatc tctgttgctt
     1441 ttttctcctt gcggagaaat gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa
     1501 aagaccttgc ttacgctggc tggacgccac cgacgcacgg tcttttcccc tctttctggc
     1561 tctgtccggc tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc
     1621 tgaccaccca caaaaatcca cggtgctgga cgcacctgac gaagcagcaa agctgctcct
     1681 gattttgcgg gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga
     1741 aaagaccgca ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct
     1801 ccggggctgc gccttttccg catggggcgg aaagaggctg gcctccgcgg tgaaagctga
     1861 acgcaatcac cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg
     1921 cagcgggggc tgcgcatcac gccggtaaac ccgtcgtctg aaggagagag attgcatgtt
     1981 taatacggat tcgcttgcct ggcagtggca tatcccggag ctggcccggc agatttaccc
     2041 gtgggtcagc tggctgtacc gggagtatta cagtctggcc ggtttttttc tggttatcgg
     2101 cctgtttttc ggtctggctg aactctgggc cggccgaatg cgttttgtgg ggctgagcca
     2161 taaggcgcgg cgggagtatg ccgatcgcta taagcgcgat aataaccgga ctgtgccatt
     2221 ttcggtgctg gcctcgctgg attcacccgg gcggatggtc agggcacggc gacatgtctg
     2281 gaactacgcg tactggctga ccggcgctgc cggtctggcc cttctggggg ggctggttgt
     2341 cgccgtgttt atcgagagct ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc
     2401 agattattac ctgctgcagc cggctaacct gttcgggata gcctggtgtg ccggagggct
     2461 tgtcgccggc gtccttctgg cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc
     2521 ggccgctgac gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg
     2581 gaccggagaa atgacagacg tccgccacct gcatttcggg gagccggtac cggttaacgc
     2641 gctggccgat ttttccacgg agcaggcccg gaaacagcag gcggtgtttc tgggtaagga
     2701 tgagcagggc cagccggtgc tggtccctcg tgatacctgg cgcaagacca atattcagat
     2761 cctcggcctg cccgggagcg gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt
     2821 acgcgatttc ggggacgctg tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca
     2881 cgtcttccgg gcgcactgcc cggacttcac cctgctggat ctgcggccgg ggaaaccggc
     2941 gcagctgaat ctgttccgcg atctcgacca gtacgccctg aagaatctgc tggttgccgg
     3001 gtttaacctg agtgagaccg gcgacgtggc ggaccactac cgcatcagcg agcagaaagc
     3061 ggcgaagctg attgcggagc agttcccgca gggggcaaac attcagcagg tgctggcggc
     3121 ggcgtacgcg ttgccggagg ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga
     3181 gaacgtggcc gacctgagcg tcctgcagac ggacagcggt atcgatgtgg cggggataat
     3241 caacggcggc ggctgcctgt acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt
     3301 gcagaagatg ctgtttgccc gctgcgcgca aatcatcatt gcgcgggatg agttccggag
     3361 gtggccgcac gcgagcatca tgctcgatga gattaaatat ctgctgtcga agtacgtgct
     3421 gaacgcgctg gggacgctgc gcagccggga ctgcaacctg ctgctcgcgc accagtcgct
     3481 gggcgacttc ggtcagtgcg ggcaggatct gcccgctgat tttgtgaaaa ccacggtact
     3541 ggataacacg ccgatccgct ggttctatcg cgcggccagc caggagtccg cgcagtgggc
     3601 ggcgggccag accggggaga tccgggtgga cgtggagcgc cgccgggcca gccgggaggc
     3661 ggggaacgtg gagcacatca gcggcgacag cttcatccag aaggaggcgc ggccgctgtt
     3721 tgacgtgaac accctgcaac acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt
     3781 ggcgcggctg gcgttcagca gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac
     3841 cttcccggtg ctggcgaaaa cggacccgct ggcggagtac ctgcctccgg ccgacggcgg
     3901 cggaccaaaa gcggatgatg attttgcggg gctttactga tgacactgga gcagtacaac
     3961 gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg
     4021 ctgcgggaag agctgtgttc cacggcggag ctggttgcgc gacggctggg gattgcggcg
     4081 gtacagccgg tttaccgctt cctggacagt ctggtggcga aagggctgct ggtccgggcg
     4141 aaatacccgg ttgacggccg tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg
     4201 ttctcgttcg acgaggacga gccgctgacg gacgttattc cgtttcagcc gtcaagggtg
     4261 tcggcggcgc agctgccgca ccggctggcc gtgcagtccc tgcgcctctc catggagggc
     4321 cggggggcca ccggctggcg gtacctgcac cgtatggccc tgaaagggat gaaagtgccg
     4381 gacgcgctgg cggagctgga cggcagaacg gtggcatttg aggtagagcg caccgtcaag
     4441 agccgcaggc ggtatcagga agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc
     4501 atcgatgaaa tctggtacat ctgccctgac cggccgacgc ttgtccgggt acagcgggcg
     4561 attctggcgg tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa aacggtggag
     4621 ctggaccggg agaggctgtt cgcgtgcttt aaatttatga caacggagtg agggattatg
     4681 gcgctgtaca tggtatttat tgaggacgtg gaagacaaag acggcgagcg ctgcattatg
     4741 ccaaccagcg agcatctgga agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt
     4801 ttctgttttg tcgaccgggc ccagcatctg gcgatgatga aatccatcgg gatgtatgac
     4861 gagcggctca acagcgacga aaaagccacg tactttttca ccggtgaaaa tccgcaatgg
     4921 gaacgggaca aacccacagg aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg
     4981 ttcgccatcg agaacggtgt gcaggtggtt gatgccggga agtgctcacc ggagcagatt
     5041 aacgcgatga ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg
     5101 aaagcggagc tgatgcggga atataacagc cgctatttta acgtgccggt gcctgaactg
     5161 ccggaggaca aacccgcccg ccggtggtgg ccgttcggca aaaaaataac gccagcaccg
     5221 gcatggccgg tgctgttatt cttccccgag cttatcccgc atcacctgcc agagtgtttc
     5281 cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta tccatgtcca gctcgttcag
     5341 cttttccagc aacagcagcg actgatcctt aatgaattta gccatattga gggcagtttt
     5401 ttcctgcttc gtcatgttct ccattccctt cggtaagttc tcagctaaac cagccggacc
     5461 agaagcgttt tttcttctgt gtcagttctg ttgtcaatct gtccacttcg gcctggagtt
     5521 tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg ggatgtttta tcttccagca
     5581 tcagggtgac agcctgccgg agatcggcca gttccttttg cattgccaga agctgttcct
     5641 gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc gggaatttgt ttctcaggga
     5701 tattcacatt tccgtataca cggatcagtt ctgacacatc cacgacaggg ttgtttttcc
     5761 cgtcacgtga cacggtaacc tttccctgtt ttacatgatt gtatagcgtt cttctggtaa
     5821 tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt tttcgccact gtatacccct
     5881 gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta aatgtatcgc tgaattttac
     5941 ccgtttttcc gttcaggatg tggttttctg acgcgctgcg cttgtcaaac cggcgacggc
     6001 cacgcaatga attgcgcccc cgtcatcggt tattagaggc tgtctgcccc cggatacccg
     6061 gattctttct gatgcctgac ggtgacgatc acaatctggt ccatttcccg gtcgtggcgg
     6121 tagagcatca catagccact gtcgccaaac ccgatcacca gttccttata ttccagcggc
     6181 agaaacggca ccgggcggcc aatgtctggc aatgttttca gttgctggat agcccggacg
     6241 atcacctcac ctccttttct ggctgccagc cgatttttgg ttttgagaaa gtcctgaagg
     6301 cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct gtggcatggc ggagcatcct
     6361 gctcgttctc agttccccag gtacttatcc aggcttccgc ttcttccgca gtaaggtgca
     6421 gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc ccgcaggtac tggctgcgct
     6481 tctcttcccg gtcgatgtat tcggtgattg cctccagcat tagcgcgtgc gctgagcggt
     6541 gccggtcatc ggccagcgtt ttcagccggt ccttaagttc ttcatcgagc ctgatggatg
     6601 ttgctgatga cataaaagcc cctttgtagt catatgtatt acacatgact actttaatcc
     6661 gtggcggcgc tatttgccag aaatacggct tttgctgccc atttttcagc ttcgcttcca
     6721 ctaatcaggg ctgctacagg ctcactcatc atccaggact attgcagcct gcgggttaaa
     6781 ggacctgtta ctggcggcaa ccatcatcat tgagcttttg ggtggtgact ggtcgcggat
     6841 cctgtaagcc gtcatttttc agactgaatt caaaatttat aacctgtcgc attgccgggt
     6901 gccctgcggg atatagccag tgcctcattg ccgtcagcgt ttcccgaaaa aaaaggtgac
     6961 agccggtaag cgacacgttg tccactcgtc catcgtccag aatatcaaac gtggcggtca
     7021 cctgaccttc gatgcgtttt gctgcggcct gtaccgggta ggttggctgc agtgtggtta
     7081 cgggttttag tggtatcgca tgtaccggag tgaggaacat ggccgccagc actgtcagag
     7141 taaaagcttt catggtctga aatccagagg ggcgtttttg aatatttgcc aggtattttc
     7201 atccggtgcc ggcggaattt tggcgtgggt aacggctgat attgctgctt tacagagttt
     7261 cggatctcct ccttcagcaa tggcgctgtt cagagtgcca tcacgttgta tggatatccg
     7321 cacggtgcac atttttcccg cataggcctc tggttcaaac atctgcattt caacagcggt
     7381 cttgatggcc gcagcgtagc tactgatttt agatgactta tccagagtat aaattttcgg
     7441 cggctgggtg gtggaatgct tacaggccgt caggagtaag gggctgcaca atgccagcag
     7501 gataaagggt attaaacgca tcgggttgat gacctgtcag taaaaaatgt tacatgtata
     7561 tttgtcactc tttacggcca cgttcattta cataaacacg gcttatcgta tcgtttttac
     7621 accaaacggc ccgccatgca gttgcgtgca taggcatgca gcgggttaca atttaccggg
     7681 cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt tatcccgtag ccgccggaaa
     7741 cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt atggtaaata ccccttaccg
     7801 gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc tcgtatttct cccgacgcat
     7861 ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca cgaagcaccc tttcccgcac
     7921 ctgttgtttt accgcctttg gcagcgtttt gttccctttc aggcccatgg ccagtgcctg
     7981 agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga gtcagtttca ggtgcgtcat
     8041 agtggcctcc gggcgtctca ggtttcccga acagtttaac cgcaggtcct ctcctcatgc
     8101 cagttagctt tcagatgtgt ctataccatc tgaaatgtaa gtgtgatgaa tgtcttaaat
     8161 aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt acaggtggct
     8221 ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca cctgaaaacg
     8281 ggaggagtaa ggtaataatt atactgtgta tatgtacagt atgttggctt taatttattg
     8341 ttttaaatgt caaagaggaa aacggtatga gtggtggaga cggtcgaggt ccgggtaatt
     8401 caggtctggg acataatggt ggtcaggcca gtgggaatgt gaacggtacg tctggtaaag
     8461 gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg ggtacgacgc
     8521 atacgccgaa tggagatatt cataactata atccggggga gtttggtaac ggcgggagta
     8581 aacccggtgg taatggtggt aacagcggca atcatagcgg tagctctggt ggcggacagt
     8641 cttcggccac tgcgatggcc ttcggtctgc ctgccctggc tactccgggg gctgaaggac
     8701 tggctttatc agtttccggc gatgcgttgt cggccgccgt tgctgatgtg ctggctgccc
     8761 tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg ctgccttctg
     8821 agattgcaaa agatgatccg aaaatgatgt caaaaattat gacgtcatta ccggccgatg
     8881 cggtgacgga gactccggca agtactttac cactggacca ggcgacggtt cgtgtcagac
     8941 aacgggttgt ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc gcaggtcggc
     9001 caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta ttcagtgtgt
     9061 cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa aggtgttccg acagcgaaag
     9121 ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg tccggctggt tttacagccg
     9181 gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag aagccggttt
     9241 atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa acagcgtcag gaggaagaaa
     9301 agcgtcgcca gcaggcatgg gacgccgctc atccggaaga ggggctgaaa agagactatg
     9361 ataaagcgaa agctgagctg gatgccgaag ataaaaatat tgcgacctta aacagccgca
     9421 ttgcatcgac agagaaggcg ctccccggtg caagggctgc tgttcaggaa gccgataaaa
     9481 aggtaaaaga ggcagaggca aataaagatg attttgtgac ttataacccg cctcatgaat
     9541 atggctccgg gtggcaggat caggttcgct atcttgataa ggatattcag aatcagaatg
     9601 agaaattaaa agcggctcag gcatctttaa acgcaatgaa tgaatcctta tccagagata
     9661 aggctgcact taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa gcgaaggatg
     9721 cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa gactacggcc
     9781 atgattatca tccagtacct aaaacagaag acataaaggg acttggagag ttaaagcgag
     9841 gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg tatggcgata
     9901 aaaaacgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg tatcgtgcca
     9961 gtgatggcga acacctaggc gcatttgatc ccaaaacggg taagcaggtt aaagggccag
    10021 atccaaaacg gaacattaaa aaatatcttt gagaggtaaa t
//
