LOCUS       NZ_CP101536             5595 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae strain FZKP4523 plasmid p2_FZKP4523, complete
            sequence.
ACCESSION   NZ_CP101536
VERSION     NZ_CP101536.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29762457
            Assembly: GCF_024397375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     To characterize the genomic characterization of K. pneumoniae
            FZKP4523
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5595)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) School of Basic Medical Science, Southwest
            Medical University, No.1 Section 1,Xiang Lin Road,Longmatan
            District, Luzhou, Sichuan 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 06:07:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,480
            Genes (coding)                    :: 5,361
            CDSs (with protein)               :: 5,361
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 47 of 119
            Pseudo Genes (incomplete)         :: 73 of 119
            Pseudo Genes (internal stop)      :: 15 of 119
            Pseudo Genes (multiple problems)  :: 16 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101536.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FZKP4523"
                     /isolation_source="no"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_FZKP4523"
                     /geo_loc_name="China:Luzhou"
                     /lat_lon="28.89 N 105.42 E"
                     /collection_date="2021"
     gene            complement(join(5484..5595,1..113))
                     /locus_tag="NNL33_RS27965"
     CDS             complement(join(5484..5595,1..113))
                     /locus_tag="NNL33_RS27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(780..992)
                     /locus_tag="NNL33_RS27970"
     CDS             complement(780..992)
                     /locus_tag="NNL33_RS27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(999..1508)
                     /locus_tag="NNL33_RS27975"
     CDS             complement(999..1508)
                     /locus_tag="NNL33_RS27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1543..1977)
                     /locus_tag="NNL33_RS27980"
                     /pseudo=""
     CDS             complement(<1543..1977)
                     /locus_tag="NNL33_RS27980"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2186..2530)
                     /locus_tag="NNL33_RS27985"
     CDS             complement(2186..2530)
                     /locus_tag="NNL33_RS27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2520..2696)
                     /locus_tag="NNL33_RS27990"
     CDS             complement(2520..2696)
                     /locus_tag="NNL33_RS27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3851..4177
                     /locus_tag="NNL33_RS27995"
     CDS             3851..4177
                     /locus_tag="NNL33_RS27995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4203..4748
                     /locus_tag="NNL33_RS28000"
     CDS             4203..4748
                     /locus_tag="NNL33_RS28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            4814..5164
                     /locus_tag="NNL33_RS28005"
     CDS             4814..5164
                     /locus_tag="NNL33_RS28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(5234..5500)
                     /locus_tag="NNL33_RS28010"
     CDS             complement(5234..5500)
                     /locus_tag="NNL33_RS28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(2783..2859)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3048..3445
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(665..2196)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccagatgcg ccagtattgc ttcccgcgca taaaacgtct ttgttctgcc ggtttgcttc
       61 gccagcgagt ccagacggga ctcaatctca tcagataacc ggatagcaag catataaacc
      121 tcccgaacac catttgctat acatgtatat caacagtacc acaatcaacc taaaagaaca
      181 aaaaataatc ttgttagccc ctcttgataa tccgtaacgc ggtcaatata tctagcttga
      241 gggctttcct cttaactatt gaaaggataa cgattatggg tcttaagccg ggtccgaaac
      301 gaattgccga ttcaacaggg gaacctgata aacgccagcg tgacaataaa aaaacgccgg
      361 gaaatactga caaattgaaa cccagcaaat cttcaaaaaa ataagtcagt agttaaaagc
      421 cctccggggc ttttttgtat ctgccgttca cgctgattaa acataacacc cgttgcccgc
      481 accgccccgg acgggttcgc cgcgaacggg gtggcagtca ggtaaaaccg gcaacaaacc
      541 acccgataaa ccgccccgtt cgcgacagat aaattttacc tggctgcata gccatgcagc
      601 caggtgaatg acatccggtt tcagcgtaaa tgcccgatga gaaccctgtt ttcctgcatt
      661 tcagcccagc gacggaccgt catagcttcg ctgccgggaa cgcgcttccg tcagttcctt
      721 ttcgtgctgc cgttgctcgt gcgcccgttc tgcggccacg gccatctcat gacgctgcac
      781 taccggctca aacgctgcag ttgcccgctc aagcgttcca cctgctgact caagcgcgtc
      841 acgcgctcgc tcagcgccgc gttctcccgt tgcataagcc ctgacatttt ccgccattcc
      901 ccgaaggcgc tctcccactc gtccagcctt ttcgagtagt cctgctgtag ctgctctaat
      961 gcgctcagca actgtttttc cagctcggtc atcggcactt actccttcac cagtctcagc
     1021 gcgttctgct tcgaccgccc gttcccgacc gtccagctct gcgtcctgtc tacggccgtc
     1081 cccttcggca gcaccagaaa acgtccgttg ctgtcttcca ggtacgtcac gccccaggtc
     1141 ttcgcgttca gcttcgacag cgctgcgttc tgccggtcga tttcggccag atttgacgct
     1201 atcaagctcc cctgatacca caacaccccg ctcaggctgg cgaacagcag tcctgacacc
     1261 atcgtgatgg tcagccatgt ccgcccgacc atacgcatca cgctcaggcg gttcgactgc
     1321 atggccgcgc tcataccctg ctcgtgggcg cggatggcgg cgctgattct cttcccgctt
     1381 tcgctcagtt ccgctctgac agacttctcg tgctcgctga atgcggcttt cagcatttcg
     1441 ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag cgttaaaaga
     1501 ctgttcatag atggctccct taagtcggat gtttcgccct ccgtccgggt cggcaatgct
     1561 gatgctgctc tttgtggtgc gcacgacctc aaaacctgcg ctttccagcg cctcagtgac
     1621 gtcctgacgc gttttaagct cccctgacga ggcgagggcc agtaagcccc gcgtaatagc
     1681 ctctgcggct tcctgcttcg ttttgggtaa cgaagatggc gtcaccagcg cgcgccggtt
     1741 ctccggtgcg ttcgggtcgt gcagccccag tcgcccgttc accacggtct gccacgcatc
     1801 gatgcgcgga cggtctgccc ggtcgtagta cggctggaga cgcctgccgg tcagcagttc
     1861 cgtgttcggt accagaaaat tcagttccag ccgccccttg tcccggtgtt caacccacag
     1921 cacgctgtac tggtctttat cgagtccggg catcagaacc cgctcgaagc tcgccatcag
     1981 cttttcacgc tggccgggcg gtaaatcctg ttcagcaaaa gacaggacgc cggaggtgta
     2041 ctttttggcg tagggcgagg cgtcgataag ctcccggacc tcctccggct ttccctgcag
     2101 gacgatggcc ccgtcgcgct ggcggtcttt ccccagcaga taatccaccg gcccggcacc
     2161 gccgcctcgc cctcgcggat gaaacttaac gatcatcgtc tgcccccttt tccagcacgg
     2221 catgccgcag ccgctcgagt tcggcatcaa tggccatcag cgcggccacc acctgaaccc
     2281 ggtcagcgcc tgaccactgg ccgccgttaa ttttccgggc gatctggttg aggttgttcc
     2341 ccatcccggc aagctggcgc agcaaaaccg gatcgatgga cggcagccgc aacgaccgcg
     2401 ccgggcgcgt atcgagacag gtttcccgca tccacaccgc cagttgcctt ccattgcagc
     2461 gctccagcag ctgctggtac tccccatcgg tcacccggat acctaaaaaa cgggtacgct
     2521 taaccgccat cctctcctcc gatatccgca aaatgtgacc gggctgaacg ctcaagctct
     2581 gccatctgct cccggcggta atcttcctcc tgtgcctgac gcatcagatt tgccatcact
     2641 gccggatgcc aggatctttt ttttacgacg agcggcttct tcttcggctt ctgcattatc
     2701 cctccttcgc aaacgccgga cggcacggaa aagttttccg ggtcgctccg gcgttggaca
     2761 agcgccagcg cgtcagtggt gttagcgggt ttcggggcgc agccctgaac cagtcacagc
     2821 gtttacgctg tgtatactgg cttactatgt tggcatgaga gcacgattca tgaaagtgcc
     2881 tcatgtggca ggtgtgatat gtggctcagg agcgtccgca ataccatgca cggacagatg
     2941 aaatgacgcc tcctcgctca ctcggtagct gcgctcctgc cgttcggctg cggcgggcgt
     3001 gaccgactca cggataacgg gataaagcaa aggcgctcaa atcagaaagg gccggagaaa
     3061 aggatgcggc gttgccgttt ttccataggc tccgcctccc ctgacgagca tcacagaaat
     3121 ggacgctcaa gtcagaggag gacgaaaccc gacaggactt aaagatgccg gtgatttctc
     3181 tccggcggct ccctcgtgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc
     3241 cgcctttttc cctttcggga agcgtggcgc tttctcatag ctcacgctgc tggtatctcg
     3301 gctcggttta ggtcgttcgc tccaagctgg gctgtatgca cgaacccccc gttcagcccg
     3361 actgctgcgc cctttccggt aactatcatc ttgagtccaa cccggacgga cacgcaaaaa
     3421 cgccactggc agcagccatt ggtaactgga tatatacgag agagatattg agaggtcttg
     3481 aagtgggtgc ctaactgcgg ctacactgaa agaacagttt tggtatctgc ggtctcgtat
     3541 acccagttac ccggttaagc agttccccaa ctgacttaac cttcgatcaa accgcctccc
     3601 caggcggttt tttcgtttac agtgcaagag attacgcgca gaaaaaaagg atctcaagaa
     3661 gatcctttaa atgatcgtca gttctgttat ccccggagct ccatccatcc cacggaaaaa
     3721 cacccgccac tgatgccctc agcgcactgc acatcatgta caaaccactc cctccctcct
     3781 tttttattgc gtttcttaat gcgcaatggt agctttgtgc gcaacaaaaa acgcaatagc
     3841 aaggggggta atgagagaaa gaacaaatat catgctagat ataggattaa agcaatcctt
     3901 acaacggctt tcgaataaaa ccaaaatgag tctaagtgat cttattaacc agagattatc
     3961 aggttcactt tcagttgaag aacgcattgg cgaaaagcct gaatggatga gaaaccttga
     4021 tccctatctc gccaatcact tttcaaatat tgagttaccg ctcccttcac cattaaaaga
     4081 ataccaagaa aaaatggatg agatagattc aattattaat gacatgatgg ttctaaagga
     4141 atatttcaga gctaagtttt acgacgaaat aaaataggca cgctatacgg aggcggagaa
     4201 ttatgttttt taaacggtat ttaacggaga aggaaatata caaggcagca ctcccaattg
     4261 ttaggcttca agggagagtt aacacttcat tacgtttttt atccaaatac tcagatgtaa
     4321 acaaaaatgg attaattgca ttgcatctta atttattaca ttttatctca tacaacatac
     4381 acggtgtaga gtctctgaaa atcactcaag caggcataaa caaagctgta caacaaagcc
     4441 ctcacgcaga tgaaataaaa aatattgcgc aggcaatagg acgcaaagga cctgagtaca
     4501 tcgaaggatt aattagaaac ccatggggga agaaaaatga taatccacta atatttataa
     4561 tgagcattga agaaagtata aaagaaaccc ttaactcagg ttatatgtta tatagagtag
     4621 ctcccataac tgagggtgaa gcagctgctt tcatggatgt tcttaaacaa ttattattat
     4681 ttgaggataa ttttcgcctt tttggtagag acttggttta taaaatagaa aaattaaaaa
     4741 tcagataaca aagtatggtg aacgaaaaat gaaagagagg atagtgaaat gtgtaatacc
     4801 aagattctca actatgtatt ggttaattgc gttcctagca tttatattcc ccggtcgatc
     4861 ttttgctgac ggttacaccg cttactaccg atgtaatggt actcaggaag catctcttac
     4921 tgttaaagat aacaaactaa gtatggttgt aggtctttct aagtttaata ggcaactgga
     4981 tacttataga ttcaaagaaa agggaatcga agtagtaatg cagagagctg cttctgatga
     5041 taactctgag gccataatgt cttttattat gcctgacgtt aacaatatga ccttattagt
     5101 taaaaataac ggctacatta tcgttaagac aacctgctgg ttaactaaaa ccgacaaaca
     5161 gtagaacacg tagttagtcg agtatttacc ataatacctg ttgtcggcat caaaaaagcc
     5221 cgcatagcgg gcttcagtcg taaacttcgc ggcgatgtcc aatcgtggcg gcaaggataa
     5281 caagctcgtc atctcggatc tcacacagaa cgcgataatc tcccacccga tagcgccaga
     5341 actcgcccag ctcacctttg agcggcttcc ctgactggcg aggatcggca gcaactgcaa
     5401 tgcgtaaact cataaaatcc acaatccgtc gtgcgttctg tttgtccatc ttgcgtaacg
     5461 atttgagcgc ccgatcggaa tagttaatcg tccagaccaa gtgacttcct cacgtcttca
     5521 gacgaatgca ctgcttcatc accacggcga acgcgtgcag cagtttctgc tgaaagataa
     5581 taatcctcca ggtct
//
