LOCUS       NZ_CP101535            10060 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae strain FZKP4523 plasmid p1_FZKP4523, complete
            sequence.
ACCESSION   NZ_CP101535
VERSION     NZ_CP101535.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29762457
            Assembly: GCF_024397375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     To characterize the genomic characterization of K. pneumoniae
            FZKP4523
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Zhang,L. and Li,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) School of Basic Medical Science, Southwest
            Medical University, No.1 Section 1,Xiang Lin Road,Longmatan
            District, Luzhou, Sichuan 646000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/08/2023 06:07:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,602
            CDSs (total)                      :: 5,480
            Genes (coding)                    :: 5,361
            CDSs (with protein)               :: 5,361
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 47 of 119
            Pseudo Genes (incomplete)         :: 73 of 119
            Pseudo Genes (internal stop)      :: 15 of 119
            Pseudo Genes (multiple problems)  :: 16 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101535.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FZKP4523"
                     /isolation_source="no"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1_FZKP4523"
                     /geo_loc_name="China:Luzhou"
                     /lat_lon="28.89 N 105.42 E"
                     /collection_date="2021"
     gene            105..1790
                     /locus_tag="NNL33_RS27900"
     CDS             105..1790
                     /locus_tag="NNL33_RS27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1800..2057
                     /locus_tag="NNL33_RS27905"
     CDS             1800..2057
                     /locus_tag="NNL33_RS27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            2142..2291
                     /locus_tag="NNL33_RS27910"
     CDS             2142..2291
                     /locus_tag="NNL33_RS27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3775..5739
                     /locus_tag="NNL33_RS27915"
     CDS             3775..5739
                     /locus_tag="NNL33_RS27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5739..6470
                     /gene="mobC"
                     /locus_tag="NNL33_RS27920"
     CDS             5739..6470
                     /gene="mobC"
                     /locus_tag="NNL33_RS27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6477..7007
                     /locus_tag="NNL33_RS27925"
     CDS             6477..7007
                     /locus_tag="NNL33_RS27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7034..7213)
                     /locus_tag="NNL33_RS27930"
     CDS             complement(7034..7213)
                     /locus_tag="NNL33_RS27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7239..7667)
                     /locus_tag="NNL33_RS27935"
     CDS             complement(7239..7667)
                     /locus_tag="NNL33_RS27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7831..8145)
                     /locus_tag="NNL33_RS27940"
     CDS             complement(7831..8145)
                     /locus_tag="NNL33_RS27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8133..8411)
                     /locus_tag="NNL33_RS27945"
     CDS             complement(8133..8411)
                     /locus_tag="NNL33_RS27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8586..8951)
                     /locus_tag="NNL33_RS27950"
     CDS             complement(8586..8951)
                     /locus_tag="NNL33_RS27950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8948..9319)
                     /gene="tolA"
                     /locus_tag="NNL33_RS27955"
     CDS             complement(8948..9319)
                     /gene="tolA"
                     /locus_tag="NNL33_RS27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9593..9838)
                     /locus_tag="NNL33_RS27960"
     CDS             complement(9593..9838)
                     /locus_tag="NNL33_RS27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..6790
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2395..2973)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5740..6467
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tattgacacc tgaaaacggg aggagtaagg taataattat actgtgtata tgtacagtat
       61 gttggcttta atttattgtt ttaaatgtca aagaggaaaa cggtatgagt ggtggagacg
      121 gtcgaggtcc gggtaattca ggtctgggac ataatggtgg tcaggccagt gggaatgtga
      181 acggtacgtc tggtaaaggt ggcccttcat caggtggtgg tacggatcca aacagcgggc
      241 cgggctgggg tacgacgcat acgccgaatg gagatattca taactataat ccgggggagt
      301 ttggtaacgg cgggagtaaa cccggtggta atggtggtaa cagcggcaat catagcggta
      361 gctctggtgg cggacagtct tcggccactg cgatggcctt cggtctgcct gccctggcta
      421 ctccgggggc tgaaggactg gctttatcag tttccggcga tgcgttgtcg gccgccgttg
      481 ctgatgtgct ggctgccctg aaggggccgt ttaagtttgg tctgtggggg attgcgatct
      541 acggtgtgct gccttctgag attgcaaaag atgatccgaa aatgatgtca aaaattatga
      601 cgtcattacc ggccgatgcg gtgacggaga ctccggcaag tactttacca ctggaccagg
      661 cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg
      721 cggttgtcgc aggtcggcca atgagtgtcc ctgtggtgga tgcgaaaccg acaaaacgtc
      781 cgggggtatt cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc gtaccgaaag
      841 gtgttccgac agcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc
      901 cggctggttt tacagccggt ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga
      961 ccggacagaa gccggtttat gtgtcggtga cagatgttct taccccggca caggtaaaac
     1021 agcgtcagga ggaagaaaag cgtcgccagc aggcatggga cgccgctcat ccggaagagg
     1081 ggctgaaaag agactatgat aaagcgaaag ctgagctgga tgccgaagat aaaaatattg
     1141 cgaccttaaa cagccgcatt gcatcgacag agaaggcgct ccccggtgca agggctgctg
     1201 ttcaggaagc cgataaaaag gtaaaagagg cagaggcaaa taaagatgat tttgtgactt
     1261 ataacccgcc tcatgaatat ggctccgggt ggcaggatca ggttcgctat cttgataagg
     1321 atattcagaa tcagaatgag aaattaaaag cggctcaggc atctttaaac gcaatgaatg
     1381 aatccttatc cagagataag gctgcactta ccggggcgat ggagagccgg aaacaaaagg
     1441 agaaaaaagc gaaggatgca gaaaataagt taaatgagga aaagaacaag cctcgcaagg
     1501 ggactaaaga ctacggccat gattatcatc cagtacctaa aacagaagac ataaagggac
     1561 ttggagagtt aaagcgaggt gatcctaaaa cacctaaaca aggtggtggt ggtaagcggg
     1621 cccgatggta tggcgataaa aaacgtaaaa tttatgaatg ggattcccag cacggtgagc
     1681 ttgaagggta tcgtgccagt gatggcgaac acctaggcgc atttgatccc aaaacgggta
     1741 agcaggttaa agggccagat ccaaaacgga acattaaaaa atatctttga gaggtaaata
     1801 tggggcttaa attacgtttg acatggtttg ataagaaaac tgaagagttt aaaggggaag
     1861 agtattctaa agactttggt gacgatggtt ctgtcattga aagcttggga atgcctttaa
     1921 aggataatat taataatggt gagtttgatt tgaagaaaga atgggttcct ttactacagc
     1981 cattttttaa aaatcaaata aaacctgatg tggataattg ttatatagct tttgattatc
     2041 gggacggtaa ctggtaacaa aaaattatca aggatgattt gaaatactga cttccggtgt
     2101 tcagggatga atgccggagt ctttaaatac agggaaagta tatgaaaaag gtaaagacta
     2161 tttttctttt tatattgatt gttgctggct ttcttctggc cgcatgtcag gcaaattata
     2221 tccgggatgt tcagggcgga acggtggcac catcgtcctc ctctgaactg acggggatcg
     2281 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctt
     2341 ttgccctgta aacgaaaaaa ccacctgggg aggtggtttg atcgaaggtt aagtcagttg
     2401 gggaactgct taaccgtggt aactggcttt cgcagagcac agcaaccaaa tctgtccttc
     2461 cagtgtagcc ggactttggc gcacacttca agagcaaccg cgtgtttagc taaacaaatc
     2521 ctctgcgaac tcccagttac caatggctgc tgccagtggc gttttgcgtg ctttcccggg
     2581 ttggactcaa gtgaacagtt accggataag gcgcagcagt cgggctgaac ggggagttct
     2641 tgcttacagc ccagcttgga gcgaacgacc tacaccgagc cgagatacca gtgtgtgagc
     2701 tatgagaaag cgccacactt cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc
     2761 agggtcggaa caggagagcg caagagggag cgacctaccg gaaacggtgg ggatctttaa
     2821 gtcctgtcgg gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga
     2881 tgcaccgaaa aagcgtctgt ttatgtgaac tctggcagga gggcggagcc tatggaaaaa
     2941 cgccaccggc gcggccctgc cgttttgcct cacatgttag tcccctgctt atccacggaa
     3001 tctgtgggta actttgtatg tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag
     3061 cgaccgagtg agcgaggagg cgcaatttcc ccggcacctg caagacggtc accggacgcg
     3121 ctgatgtggg atattgtccc ctgattaagt ctttctattc cctgacgatc aattatcagg
     3181 ataggtgaac catgtcggtt tttcgctgaa cattttacgt taacctatct ctgttgcttt
     3241 tttctccttg cggagaaatg caagtcatcc ccgctggggc gtggctgact tcaggggaaa
     3301 agaccttgct tacgctggct ggacgccacc gacgcacggt cttttcccct ctttctggct
     3361 ctgtccggct ggacgccggc cacgccagaa attcacccgg cccccgtcag gctcacgcct
     3421 gaccacccac aaaaatccac ggtgctggac gcacctgacg aagcagcaaa gctgctcctg
     3481 attttgcggg tggtcagccg ctcgctatgg cgaactctga cgggggccgg aacaccggaa
     3541 aagaccgcac tggggtgccg ccttttcctc tcacacgctg gggcgtgctc gttgttcctc
     3601 cggggctgcg ccttttccgc atggggcgga aagaggctgg cctccgcggt gaaagctgaa
     3661 cgcaatcacc acggacgtga ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc
     3721 agcgggggct gcgcatcacg ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt
     3781 aatacggatt cgcttgcctg gcagtggcat atcccggagc tggcccggca gatttacccg
     3841 tgggtcagct ggctgtaccg ggagtattac agtctggccg gtttttttct ggttatcggc
     3901 ctgtttttcg gtctggctga actctgggcc ggccgaatgc gttttgtggg gctgagccat
     3961 aaggcgcggc gggagtatgc cgatcgctat aagcgcgata ataaccggac tgtgccattt
     4021 tcggtgctgg cctcgctgga ttcacccggg cggatggtca gggcacggcg acatgtctgg
     4081 aactacgcgt actggctgac cggcgctgcc ggtctggccc ttctgggggg gctggttgtc
     4141 gccgtgttta tcgagagctg gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca
     4201 gattattacc tgctgcagcc ggctaacctg ttcgggatag cctggtgtgc cggagggctt
     4261 gtcgccggcg tccttctggc gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg
     4321 gccgctgacg aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag cagccagcgg
     4381 accggagaaa tgacagacgt ccgccacctg catttcgggg agccggtacc ggttaacgcg
     4441 ctggccgatt tttccacgga gcaggcccgg aaacagcagg cggtgtttct gggtaaggat
     4501 gagcagggcc agccggtgct ggtccctcgt gatacctggc gcaagaccaa tattcagatc
     4561 ctcggcctgc ccgggagcgg caagagcgcg atggccacca acgcgctcat tcgctgcgta
     4621 cgcgatttcg gggacgctgt ggtgtacttc gaccccaaag gggacgcgtg ggcgccgcac
     4681 gtcttccggg cgcactgccc ggacttcacc ctgctggatc tgcggccggg gaaaccggcg
     4741 cagctgaatc tgttccgcga tctcgaccag tacgccctga agaatctgct ggttgccggg
     4801 tttaacctga gtgagaccgg cgacgtggcg gaccactacc gcatcagcga gcagaaagcg
     4861 gcgaagctga ttgcggagca gttcccgcag ggggcaaaca ttcagcaggt gctggcggcg
     4921 gcgtacgcgt tgccggaggc cctgaaaaag gacgtgaagg ggctgatcac caagctggag
     4981 aacgtggccg acctgagcgt cctgcagacg gacagcggta tcgatgtggc ggggataatc
     5041 aacggcggcg gctgcctgta cgtcatcggc tcgatggacg atgaggcggt gatccgggtg
     5101 cagaagatgc tgtttgcccg ctgcgcgcaa atcatcattg cgcgggatga gttccggagg
     5161 tggccgcacg cgagcatcat gctcgatgag attaaatatc tgctgtcgaa gtacgtgctg
     5221 aacgcgctgg ggacgctgcg cagccgggac tgcaacctgc tgctcgcgca ccagtcgctg
     5281 ggcgacttcg gtcagtgcgg gcaggatctg cccgctgatt ttgtgaaaac cacggtactg
     5341 gataacacgc cgatccgctg gttctatcgc gcggccagcc aggagtccgc gcagtgggcg
     5401 gcgggccaga ccggggagat ccgggtggac gtggagcgcc gccgggccag ccgggaggcg
     5461 gggaacgtgg agcacatcag cggcgacagc ttcatccaga aggaggcgcg gccgctgttt
     5521 gacgtgaaca ccctgcaaca cctgcctgac gggttcgcgg tgatgaccgg gctgggcgtg
     5581 gcgcggctgg cgttcagcag cccgctgcgg gttgagcgcc gggaaatttc gctgaaaacc
     5641 ttcccggtgc tggcgaaaac ggacccgctg gcggagtacc tgcctccggc cgacggcggc
     5701 ggaccaaaag cggatgatga ttttgcgggg ctttactgat gacactggag cagtacaacg
     5761 tcagccacgc gaagcggcag gcgcggaacg cggagaaaac acggctgacc ctgcgctggc
     5821 tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg acggctgggg attgcggcgg
     5881 tacagccggt ttaccgcttc ctggacagtc tggtggcgaa agggctgctg gtccgggcga
     5941 aatacccggt tgacggccgt caggtcagcg tgtgggggct gacgccgcac ggggtggcgt
     6001 tctcgttcga cgaggacgag ccgctgacgg acgttattcc gtttcagccg tcaagggtgt
     6061 cggcggcgca gctgccgcac cggctggccg tgcagtccct gcgcctctcc atggagggcc
     6121 ggggggccac cggctggcgg tacctgcacc gtatggccct gaaagggatg aaagtgccgg
     6181 acgcgctggc ggagctggac ggcagaacgg tggcatttga ggtagagcgc accgtcaaga
     6241 gccgcaggcg gtatcaggaa gtggtgtccg ggtatctgtt caaccgccgg gcgaacggca
     6301 tcgatgaaat ctggtacatc tgccctgacc ggccgacgct tgtccgggta cagcgggcga
     6361 ttctggcggt ggatgaaatc gtgaatccac agaccggcga ggcccgcaaa acggtggagc
     6421 tggaccggga gaggctgttc gcgtgcttta aatttatgac aacggagtga gggattatgg
     6481 cgctgtacat ggtatttatt gaggacgtgg aagacaaaga cggcgagcgc tgcattatgc
     6541 caaccagcga gcatctggaa gacatggtgg tgctcgcctg gcagaccggg gcgtatgttt
     6601 tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg atgtatgacg
     6661 agcggctcaa cagcgacgaa aaagccacgt actttttcac cggtgaaaat ccgcaatggg
     6721 aacgggacaa acccacagga atgaagctgt gtgattacct ctatcgaaag gtggagcggt
     6781 tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg gagcagatta
     6841 acgcgatgat tgaggtggtc agcgagcggc acggggaaag cgcggtgaac gagaagctga
     6901 aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggtg cctgaactgc
     6961 cggaggacaa acccgcccgc cggtggtggc cgttcggcaa aaaataacgc cagcaccggc
     7021 atggccggtg ctgttattct tccccgagct tatcccgcat cacctgccag agtgtttccg
     7081 catcctcgtg aagccgttcg cacatgtcgg ccggggtatc catgtccagc tcgttcagct
     7141 tttccagcaa cagcagcgac tgatccttaa tgaatttagc catattgagg gcagtttttt
     7201 cctgcttcgt catgttctcc attcccttcg gtaagttctc agctaaacca gccggaccag
     7261 aagcgttttt tcttctgtgt cagttctgtt gtcaatctgt ccacttcggc ctggagtttc
     7321 cggcgctcat catcgtgctg gcggcgttct tcttcccggg atgttttatc ttccagcatc
     7381 agggtgacag cctgccggag atcggccagt tccttttgca ttgccagaag ctgttcctgt
     7441 gggaaatttt tttgtgtatt ttcacggtgt gaaatgccgg gaatttgttt ctcagggata
     7501 ttcacatttc cgtatacacg gatcagttct gacacatcca cgacagggtt gtttttcccg
     7561 tcacgtgaca cggtaacctt tccctgtttt acatgattgt atagcgttct tctggtaatg
     7621 cccgcggctt gtgctgcctg tgaaaggttg agcaatgttt tcgccactgt atacccctga
     7681 ttttatcggg tgtgaaaggt gtgtaatttc tcacggtaaa tgtatcgctg aattttaccc
     7741 gtttttccgt tcaggatgtg gttttctgac gcgctgcgct tgtcaaaccg gcgacggcca
     7801 cgcaatgaat tgcgcccccg tcatcggtta ttagaggctg tctgcccccg gatacccgga
     7861 ttctttctga tgcctgacgg tgacgatcac aatctggtcc atttcccggt cgtggcggta
     7921 gagcatcaca tagccactgt cgccaaaccc gatcaccagt tccttatatt ccagcggcag
     7981 aaacggcacc gggcggccaa tgtctggcaa tgttttcagt tgctggatag cccggacgat
     8041 cacctcacct ccttttctgg ctgccagccg atttttggtt ttgagaaagt cctgaaggcg
     8101 ttgtaaatcg cgctgtgcca gcgcggaaat cgttacctgt ggcatggcgg agcatcctgc
     8161 tcgttctcag ttccccaggt acttatccag gcttccgctt cttccgcagt aaggtgcaga
     8221 cccgtttcct gatagtgctg ccatgcggcc tgtccgtccc gcaggtactg gctgcgcttc
     8281 tcttcccggt cgatgtattc ggtgattgcc tccagcatta gcgcgtgcgc tgagcggtgc
     8341 cggtcatcgg ccagcgtttt cagccggtcc ttaagttctt catcgagcct gatggatgtt
     8401 gctgatgaca taaaagcccc tttgtagtca tatgtattac acatgactac tttaatccgt
     8461 ggcggcgcta tttgccagaa atacggcttt tgctgcccat ttttcagctt cgcttccact
     8521 aatcagggct gctacaggct cactcatcat ccaggactat tgcagcctgc gggttaaagg
     8581 acctgttact ggcggcaacc atcatcattg agcttttggg tggtgactgg tcgcggatcc
     8641 tgtaagccgt catttttcag actgaattca aaatttataa cctgtcgcat tgccgggtgc
     8701 cctgcgggat atagccagtg cctcattgcc gtcagcgttt cccgaaaaaa aaggtgacag
     8761 ccggtaagcg acacgttgtc cactcgtcca tcgtccagaa tatcaaacgt ggcggtcacc
     8821 tgaccttcga tgcgttttgc tgcggcctgt accgggtagg ttggctgcag tgtggttacg
     8881 ggttttagtg gtatcgcatg taccggagtg aggaacatgg ccgccagcac tgtcagagta
     8941 aaagctttca tggtctgaaa tccagagggg cgtttttgaa tatttgccag gtattttcat
     9001 ccggtgccgg cggaattttg gcgtgggtaa cggctgatat tgctgcttta cagagtttcg
     9061 gatctcctcc ttcagcaatg gcgctgttca gagtgccatc acgttgtatg gatatccgca
     9121 cggtgcacat ttttcccgca taggcctctg gttcaaacat ctgcatttca acagcggtct
     9181 tgatggccgc agcgtagcta ctgattttag atgacttatc cagagtataa attttcggcg
     9241 gctgggtggt ggaatgctta caggccgtca ggagtaaggg gctgcacaat gccagcagga
     9301 taaagggtat taaacgcatc gggttgatga cctgtcagta aaaaatgtta catgtatatt
     9361 tgtcactctt tacggccacg ttcatttaca taaacacggc ttatcgtatc gtttttacac
     9421 caaacggccc gccatgcagt tgcgtgcata ggcatgcagc gggttacaat ttaccgggcg
     9481 cgtttccggc ggttttccgg gtggtttgtt gcctgtttta tcccgtagcc gccggaaacg
     9541 ccctcagacc gtctgagcgg tgcgggcaat gcgacgttat ggtaaatacc ccttaccggc
     9601 ggcgctgtgg ctgccaggtg aaggtggttt ccgtagcctc gtatttctcc cgacgcattt
     9661 cctgcgcccg cctggcttca cgttcgtcct gaatggcacg aagcaccctt tcccgcacct
     9721 gttgttttac cgcctttggc agcgttttgt tccctttcag gcccatggcc agtgcctgag
     9781 cgacaaaggc gtccgccagg cgcttttctt cagcgggagt cagtttcagg tgcgtcatag
     9841 tggcctccgg gcgtctcagg tttcccgaac agtttaaccg caggtcctct cctcatgcca
     9901 gttagctttc agatgtgtct ataccatctg aaatgtaagt gtgatgaatg tcttaaataa
     9961 taaaatttgt tgccatgaga aattttcggg aaaagacgcg atctgcttac aggtggctcc
    10021 gggcgctttt ttgtgcctga agatgagaac taatctcata
//
