LOCUS       NZ_CP101296             6741 bp    DNA     circular CON 27-JUL-2023
DEFINITION  Escherichia coli strain 2003-3014 plasmid pO26-2, complete sequence.
ACCESSION   NZ_CP101296
VERSION     NZ_CP101296.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24907326
            Assembly: GCF_024300685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Kalalah,A. and Eppinger,M.
  TITLE     Whole genome sequencing, comparative phylogenetic analysis, and
            phenotypic profiling of the non-O157 Big Six Shiga toxin-producing
            Escherichia coli strains obtained from ATCC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   Kalalah,A. and Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Molecular Microbiology and Immunology, The
            University of Texas at San Antonio, One UTSA Circle, San Antonio, TX
            78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2022
            Assembly Method :: Unicycler v. Galaxy Version 0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 296x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/27/2023 05:39:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,128
            CDSs (total)                      :: 5,990
            Genes (coding)                    :: 5,666
            CDSs (with protein)               :: 5,666
            Genes (RNA)                       :: 138
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 132 of 324
            Pseudo Genes (incomplete)         :: 191 of 324
            Pseudo Genes (internal stop)      :: 73 of 324
            Pseudo Genes (multiple problems)  :: 66 of 324
            Pseudo Genes (short protein)      :: 3 of 324
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2003-3014"
                     /serotype="O26:H11"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:BAA-2196"
                     /db_xref="taxon:562"
                     /plasmid="pO26-2"
                     /collection_date="2021"
                     /note="ATCC lot#70005699"
     gene            complement(426..1583)
                     /locus_tag="NMK58_RS30460"
                     /old_locus_tag="NMK58_30460"
     CDS             complement(426..1583)
                     /locus_tag="NMK58_RS30460"
                     /old_locus_tag="NMK58_30460"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219937.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:1904047 -
                     S-adenosyl-L-methionine binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001219937.1"
                     /translation="MRIIDLFSGCGGLSLGFLKGGFDVVGAYDFWDPAIECYRDNFSHP
                     IKKLDLSNVDDVVRELKDIDFDMIIGGPPCQDFSHAGLRIEGARANLTRSFSEIIKRIK
                     PKWFVMENVDRALRSGAYLEARGIFKESGYGLTEIVLDASKCGVPQKRKRLFVIGKLDV
                     RDGFILNEVMCGISKDSMTVRNYLGDSLGIEYYYRHPRNYNRRAIFSIDEPAPTVRGVN
                     RPIPDGYLGHAGDPVSISENVRPLTTFERARLQTFPEDFKFKGAKTNLEQMIGNAVPVE
                     LAKYVAVTIMEYEKKQVKGIYDKEGFRAWLLNEKKLTKRTSSDIISRCCRGVSFFDSEG
                     VDFYNCEIDEIIMKLERLESFVRLGVSLKSQLRRAFKLYYEYCRR"
                     /name="DNA cytosine methyltransferase"
     gene            1671..2732
                     /locus_tag="NMK58_RS30465"
                     /old_locus_tag="NMK58_30465"
     CDS             1671..2732
                     /locus_tag="NMK58_RS30465"
                     /old_locus_tag="NMK58_30465"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034299.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009036 - type II site-specific deoxyribonuclease
                     activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HindVP family restriction endonuclease"
                     /protein_id="WP_000034299.1"
                     /translation="MSKIESLYGLKNTNRKESDLWGKNQFNSSFPTSLACYMRDNKMNP
                     IYLYLDKDLKVHAKEVSFDFVFNTTLKNSDLEFQFEKKYEPYQQYAYDDIKGIDLVIKG
                     NNSFLKALEIKLTVLPDSTTYKENESEWSSEIVIRPASTSYCALGIMHSCSDKFHEIRK
                     IFESTCESIQHWGNETEVLSKKENILNCLNTFQENFLDYQKPFLMQPLWKTKGKSPILD
                     QNAFDIFIWSDFALCRTFIDRSSYNNSSASRLMRSSARLARMVYEIASKNKTNINRIYT
                     EMNFGYQSDKEFSLNGKITKAYMDHPRRTKPILPPSVLKEIILNGGQNKLSPERRFDQS
                     VYYTASHLFDEIK"
                     /name="HindVP family restriction endonuclease"
     gene            3123..3506
                     /locus_tag="NMK58_RS30470"
                     /old_locus_tag="NMK58_30470"
     CDS             3123..3506
                     /locus_tag="NMK58_RS30470"
                     /old_locus_tag="NMK58_30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000065853.1"
                     /translation="MSNIVHLSDKKQKNKPVDISFDDYTDNLSKGFVKNLSNKQKKELI
                     SKIKAILKSRNVAFRSTPPSDWKEKSRTFAIFFPEKKIVFTLGVVNKLKIEDGWINHKI
                     SDTEDAVLSVGKIINLIEDSQHG"
                     /name="hypothetical protein"
     gene            3499..3705
                     /locus_tag="NMK58_RS30475"
                     /old_locus_tag="NMK58_30475"
     CDS             3499..3705
                     /locus_tag="NMK58_RS30475"
                     /old_locus_tag="NMK58_30475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096539.1"
                     /translation="MAKLPQEKPKEVIQAGFDDVKKAYKKGAKTVNDTYAEQAGRDLQS
                     GDGKLMATYRRNKKAIDKLDAEG"
                     /name="hypothetical protein"
     gene            complement(4080..4313)
                     /locus_tag="NMK58_RS30480"
                     /old_locus_tag="NMK58_30480"
     CDS             complement(4080..4313)
                     /locus_tag="NMK58_RS30480"
                     /old_locus_tag="NMK58_30480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001448503.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSERTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4320..4838)
                     /locus_tag="NMK58_RS30485"
                     /old_locus_tag="NMK58_30485"
     CDS             complement(4320..4838)
                     /locus_tag="NMK58_RS30485"
                     /old_locus_tag="NMK58_30485"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001447511.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4876..5307)
                     /locus_tag="NMK58_RS30490"
                     /old_locus_tag="NMK58_30490"
                     /pseudo=""
     CDS             complement(<4876..5307)
                     /locus_tag="NMK58_RS30490"
                     /old_locus_tag="NMK58_30490"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5516..5839)
                     /locus_tag="NMK58_RS30495"
                     /old_locus_tag="NMK58_30495"
     CDS             complement(5516..5839)
                     /locus_tag="NMK58_RS30495"
                     /old_locus_tag="NMK58_30495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955994.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIEGELRSLRQVVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5904..6095
                     /locus_tag="NMK58_RS30500"
                     /old_locus_tag="NMK58_30500"
     CDS             5904..6095
                     /locus_tag="NMK58_RS30500"
                     /old_locus_tag="NMK58_30500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165984.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGEDL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6174..6258)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          2..149
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.644"
                     /coverage="100.0"
                     /evalue="1.48e-62"
     Typing          6314..6741
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.2"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3983..5526)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.529"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5523..5872)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.162"
                     /coverage="99.0"
                     /evalue="1.96e-64"
ORIGIN
        1 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
       61 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacacgac
      121 ttaacgccac tggcagcagc cactggtaac tggacaaggt ggattgagat attaagagtt
      181 cttgaagtgg tggcctaact gcggctacac tagaaggaca gtatttggta tctgcgctcc
      241 accaagccag ttaccttcgg aagaaagagt tggtagctct agattccggc aaacaaacca
      301 ccgttggtag cggtggtttt tttgtttaca ggcagcagat tacgcgcaga aaaaaaggat
      361 cacaagagga tcctttgatc tttttatccc ctccccctcc cctgagagac ataaattcgt
      421 atttattacc ttcgacaata ttcgtaatat aatttaaaag cccttcttaa ctgagacttc
      481 aggctaaccc caagcctgac aaacgattca agcctttcca gtttcataat aatttcatct
      541 atttcacagt tataaaaatc caccccctct gaatcaaaaa atgaaacacc tctgcaacat
      601 ctagatataa tatcgctaga ggttcttttt gttagtttct tttcattcag caaccacgcc
      661 ctaaagcctt ctttatcata aatacctttt acttgttttt tttcatactc catgattgtc
      721 accgcaacat attttgctaa ctcaaccgga acagcattac ctatcatttg ctccaaattt
      781 gtttttgcac ctttaaattt aaagtcttca ggaaatgttt gcaatcttgc tctttcaaag
      841 gttgttaatg gccttacatt ctcagaaata ctgacaggat cgcctgcatg ccctaaatat
      901 ccatcaggta tcggtctatt aacccctctt actgttggcg caggttcatc aatagagaaa
      961 atagcccttc tgttataatt tctcggatgt ctgtaataat actcaatccc caatgaatca
     1021 ccaagataat ttctaactgt catactatct ttcgatatcc cgcacatcac ttcgttaagg
     1081 ataaaaccat ccctcacgtc aagctttcca attacaaata atctttttct tttttgcgga
     1141 acaccacact tactggcatc caaaacaatt tctgttaacc cataccctga ttctttaaag
     1201 attcctcgag cttctaaata ggcaccactt cttaatgccc gatctacatt ttccatcaca
     1261 aaccactttg gctttattct ttttataatt tctgaaaatg agcgagttaa gttagctcta
     1321 gcaccttcaa ttcttaaacc tgcatgactg aagtcttggc aaggaggccc tccaataatc
     1381 atatcgaagt ctatatcttt aagctccctc accacatcat caacattgct caaatctagt
     1441 ttttttatcg gatggctaaa attatctctg taacattcaa tagcaggatc ccagaaatca
     1501 taagcaccta caacatcaaa cccccccttc aaaaagccta gggacaaccc cccacaccca
     1561 ctaaaaagat caattattct cataacataa aaaactcatt atcatatcac tccacatcat
     1621 gatatagaaa cacaccatag acgtcaataa attgacgtca ggagcaaaaa gtgagcaaaa
     1681 tagaatctct ttatggttta aaaaacacca acaggaaaga gagcgatctg tggggaaaga
     1741 accaatttaa cagttctttt cccacctctc tagcatgtta tatgagagac aacaaaatga
     1801 atccgattta tctttattta gataaggatt taaaagtgca tgccaaagaa gtatcttttg
     1861 acttcgtttt taacacaaca ttaaaaaata gtgatcttga atttcaattt gagaaaaaat
     1921 atgaaccata ccaacagtat gcttatgatg acataaaagg aatagatcta gttataaaag
     1981 gaaacaatag ttttttaaaa gcactagaaa taaaattaac cgtacttcca gatagcacga
     2041 catataaaga aaacgagagc gaatggagtt cagagattgt aataagacct gcaagcactt
     2101 cttattgtgc ccttggcata atgcactctt gcagcgataa attccatgaa ataagaaaaa
     2161 tatttgaatc cacatgcgaa agcatacagc actgggggaa tgaaactgaa gttctttcaa
     2221 agaaagaaaa tatattaaat tgcctaaaca ctttccaaga aaattttctt gattaccaga
     2281 aacctttttt aatgcaacca ttatggaaaa caaaaggcaa gagcccaata ttagatcaaa
     2341 atgcctttga catattcatc tggagtgatt ttgctttgtg tcgaaccttt attgatagat
     2401 cctcatataa caattcatct gccagcagac taatgagatc atcagccaga ctcgccagaa
     2461 tggtgtatga aattgcgagc aaaaacaaga caaacatcaa tagaatttat actgagatga
     2521 actttggata tcaaagtgat aaagagttct cgctaaacgg aaaaataaca aaagcttata
     2581 tggatcaccc aagaagaaca aaacccatcc tcccaccatc agtattaaaa gaaataattt
     2641 taaatggcgg gcaaaataaa ttaagcccag agagacgctt tgatcaaagc gtttattata
     2701 ctgcatctca cctgtttgat gaaataaagt aatttttctt ggtggctcgc cagccctgaa
     2761 ttcgcccatc aaaaatcagt agcggaacag gcatactaga catgctcgct tttacggcga
     2821 gcatcttgac ggagtttgag acgcaatgga acagcaacca actctatgca tcacaagctc
     2881 aaaaatgatt accatgcatt gaactctagt tacctttgcc aatgattgct cctggttgct
     2941 tcatgcccgt aaaggcaatt tcgacggtca ttacattgaa cataacatct cgaccgcgta
     3001 ctgaacggtc tcagccggat cttcgaaccc atctatcgta gaattgatac tctacctctg
     3061 tgactaacaa gacagttccg ctactcttct gagtattcat tcatcaaaaa gcaggatatc
     3121 cagtgtcaaa cattgttcat ctatctgata aaaaacaaaa aaacaaacca gttgatattt
     3181 cttttgatga ttacacagac aatctcagca aagggttcgt caaaaatctc agcaataaac
     3241 aaaaaaaaga attaattagt aaaatcaaag cgatacttaa atcgaggaat gtagcgttcc
     3301 gttcgacgcc gccctctgat tggaaagaga aaagtagaac ctttgcaatt ttctttcctg
     3361 aaaaaaagat tgtttttaca ttaggagtag tgaacaaatt aaagatagag gacggttgga
     3421 taaatcataa aatcagcgac acagaagatg ccgtcttatc tgttggaaaa ataatcaacc
     3481 ttatagagga ttcacagcat ggctaaatta cctcaagaaa agccgaaaga agtcatccaa
     3541 gccggatttg atgatgttaa aaaggcctat aaaaaaggcg ctaagactgt aaacgatacc
     3601 tatgctgagc aagcaggtcg tgacttacaa tcaggcgatg gtaaactaat ggctacctat
     3661 cggcgcaata aaaaagcgat agacaagtta gatgctgaag gttgatctga aaagtcagtc
     3721 ttctgggcgg acttttcgtc tctaagactg gctgcatatt gtatttacca taacatccgt
     3781 tacgcacacc gctaaaacgc gttcggtgcg atcacggcag cagacaggta aaaatggcaa
     3841 caaaccaccc gaaaaactgc cgcgatcgcg cctgataaat tttaaccgca tgaataccta
     3901 tgcaaccaga tggtgcagac tacattagtc cgtcaaaatc cactgaacct gccatttttt
     3961 atagtttcgc catcacagcg acaacgaagg accatccagt atgcgttcct gtatttccgg
     4021 ctgacgctcc cgttccaggg ataacgcatg ttcgcgctcc tgtatcagcc gttcctctct
     4081 tatctccagt tctcgctgta taactggctc aagcgttctg tccgctcgct caagtgctgc
     4141 acctgctgac tcaactgcat gacccgctcg ttcagcatcg cgttgtcccg ttgcgtaagc
     4201 gaaaacatct tctgcaattc cacgaaggcg ctctcccatt cgttcagccg ctgcatatag
     4261 tcctgttgca gctgctctaa ggcgttcagc aaatgtgttt ccagctctgt cattccgtat
     4321 cactccgtca gttgtatcca gtccttcccc tgatagtgcg tcacctctgc tgacatccct
     4381 tgcggaatga ccagaaattt ccttctcccg tcctgcacaa actccacgcc ccatgtcttc
     4441 gcgttcagtt tctgcaatgt ctcttcctgc ttcctgattt cttccaggtt cgcctgtatc
     4501 ctcccgccaa gataccagag cgtcccgcca ctcgcggtaa acaggagaaa gactatcccc
     4561 agtaacatca tgcccgtatt ccctgccagc tttaacacgt ccttcctgtg ctgcatcatc
     4621 gcctctttca ccccttccct gtgtttttcc agcgatttct ctgtcgaggc tgtgaacagg
     4681 gctatagcgt ctctgatttt cgtctcgttt gaggtcacag cctcgctgac agactcgccg
     4741 agcttcctga actcgtcgtt cagcattttc tctgtagatt cggctctctc tttcagcttt
     4801 ttctcgaact ccgcgcccgt ctgtaaaaga ttgctcataa aacgctcctt tcagcctgat
     4861 attcttcccg ccgttcgggt ctgcaatgct gatgctgctt cgcgtcaccc tgaccacttc
     4921 aagccccgcc tctgtgagcg cctgaatcac atcctgacgg ccttttatct cccctacatg
     4981 ataaagagcg tctatcccgc gtgtgacgct ctcagcaagc gcctgtttcg tttccggcag
     5041 gttatcagag agagtcagta tccggcggtt ctccggggcg ttggggtcat gcagcccgta
     5101 atggtgattc accagtgtct gccaggcatc aattctcggc ctgtctgctc ggtcgtagta
     5161 cggctggagg cgttttccgg tctgtagctc catgttcgga atgacaaaat tcagctcaag
     5221 acgccccttg tcctggtgct ccacccacag aatgctgtac tgattttttt cgagaccggg
     5281 catcagtacg cgctcaaagc tctccatcac tttttcacgt cctcccggcg gcagttcctt
     5341 ctccgcgaac gacagaacac cggacgtgta tttcttcgca aatggcgtgg catcgatgag
     5401 ttccctgact tcttccggat taccctgaag caccgttgcc ccttcccggt tacgctccct
     5461 tcccagcagg taatcaaccg gaccactgcc accgcctttt cccctggcat gaaatttaac
     5521 aatcatcccg cgctccctgc tccctgacga cctgccgtaa gctgcgcaac tctccctcga
     5581 tggccatcag tgcggccacc acctgaaccc ggtcaccgga agaccactgc ccgctgttta
     5641 ccttcctcgc tgtctgattc aggttattcc cgatggcggc cagctgacgc agtaacggcg
     5701 gtgccagtgt cggcagcttc ccggaacggg caaccggctc acccaggcag acccgccgca
     5761 tccataccgc cagttgttta ccctcacagc gttccagtaa ccgggcatgt tcatcatcag
     5821 taacccgtat cgtgagcatc ctctcgcgtt tcatcggtat cattaccccc atgaacagaa
     5881 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     5941 gctttatcag aagccagaca ttaacgctgc tggagaaact caacgaactg gacgcagatg
     6001 aacaggccga tatttgtgaa tctcttcacg accacgccga tgagctttac cgcagctgcc
     6061 tcgcgcgttt cggtgatgac ggtgaagacc tctgacacat gcagctcccg gagacggtca
     6121 cagcttgcct gtgagcggat gccgggagca gacaagcccg tcagggcgcg tcagggggtt
     6181 tcggggcgaa gccctgaacc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     6241 gtggcatcag tgcggattat aagaaaagag caccatatgc ggtgtgaaat accgcacaga
     6301 tgcgtaagga gaaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     6361 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggagtcgg
     6421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataaggcc ggaaagaaca
     6481 tgtgagcaaa aaggcaaagg acaacaaaag gccgcgccgc tggcgttttt ccataggctc
     6541 cgcccccctg acgagcatca cagaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6721 catagctcac gctgttggta t
//
