LOCUS       NZ_CP101293             5176 bp    DNA     circular CON 27-JUL-2023
DEFINITION  Escherichia coli strain 2003-3014 plasmid pCol156-O26-5, complete
            sequence.
ACCESSION   NZ_CP101293
VERSION     NZ_CP101293.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN24907326
            Assembly: GCF_024300685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Kalalah,A. and Eppinger,M.
  TITLE     Whole genome sequencing, comparative phylogenetic analysis, and
            phenotypic profiling of the non-O157 Big Six Shiga toxin-producing
            Escherichia coli strains obtained from ATCC
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Kalalah,A. and Eppinger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Molecular Microbiology and Immunology, The
            University of Texas at San Antonio, One UTSA Circle, San Antonio, TX
            78249, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2022
            Assembly Method :: Unicycler v. Galaxy Version 0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 296x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/27/2023 05:39:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,128
            CDSs (total)                      :: 5,990
            Genes (coding)                    :: 5,666
            CDSs (with protein)               :: 5,666
            Genes (RNA)                       :: 138
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 105
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 132 of 324
            Pseudo Genes (incomplete)         :: 191 of 324
            Pseudo Genes (internal stop)      :: 73 of 324
            Pseudo Genes (multiple problems)  :: 66 of 324
            Pseudo Genes (short protein)      :: 3 of 324
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101293.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2003-3014"
                     /serotype="O26:H11"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:BAA-2196"
                     /db_xref="taxon:562"
                     /plasmid="pCol156-O26-5"
                     /collection_date="2021"
                     /note="ATCC lot#70005699"
     gene            1..936
                     /locus_tag="NMK58_RS29930"
                     /old_locus_tag="NMK58_29930"
     CDS             1..936
                     /locus_tag="NMK58_RS29930"
                     /old_locus_tag="NMK58_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_024242392.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(1130..1528)
                     /locus_tag="NMK58_RS29935"
                     /old_locus_tag="NMK58_29935"
     CDS             complement(1130..1528)
                     /locus_tag="NMK58_RS29935"
                     /old_locus_tag="NMK58_29935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024017382.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDQIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1705..2631
                     /locus_tag="NMK58_RS29940"
                     /old_locus_tag="NMK58_29940"
     CDS             1705..2631
                     /locus_tag="NMK58_RS29940"
                     /old_locus_tag="NMK58_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001399791.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELIRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2634..3167
                     /locus_tag="NMK58_RS29945"
                     /old_locus_tag="NMK58_29945"
     CDS             2634..3167
                     /locus_tag="NMK58_RS29945"
                     /old_locus_tag="NMK58_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3233..3442)
                     /locus_tag="NMK58_RS29950"
                     /old_locus_tag="NMK58_29950"
     CDS             complement(3233..3442)
                     /locus_tag="NMK58_RS29950"
                     /old_locus_tag="NMK58_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          2..936
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.261"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1706..2628
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgaagaa aatttacccc accgtcccta tcacacggat
       61 gatctcgctt tcggccttcg tatctccggc aaagggcgcg cgcttcttgc acggtatatc
      121 cagcagaacc agcctcatgc acagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgctccc gcgccgaaca tcaccgttaa aaatcctgtg
      241 aacggacacg ctcacctgct ctacgcactc aacatcgccg tgagaaccgc gcctgatgcc
      301 tcggttaagg cgctgaaata tgccgcagcg gttgaacgtt cgctgtgtga gaaactttgt
      361 gcggatgtga actacagcgg cctgatttgc aaaaatccgt tccacctgga atggctggtg
      421 atggagtggc gcgaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc
      481 gcctcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggccagc gttctcacag
      601 tggcttgaag cggtgatcca gcgtgtcgaa atgtacaatg catcgcttcc cgttccgctt
      661 tcactggctg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat acaggccaag
      781 agaggcagga aaggtggcat cgctaaaggc gaagcctacg atgacaagcg tttcatggcg
      841 ctatgtatgc tggagaatgg atattctcag aaagctattg cggcgatgtt ggaggtttct
      901 actcgaacca ttcgaaactg gaaaagcgga aaatagccta tatcagataa cagcgccttt
      961 ctggcgtctt tttgagcagc aggttttttg ccgtttttcc ccgttcagcc ctgcaatggc
     1021 gcggctttcc gcgtgattgt ggtggtagtg ctcgccgcag tctcatgacc gagcgtagcg
     1081 agcgaatgag cgaggaagca ccagggaatc ggaacacgga tggaggcact tacgcgtctg
     1141 ggtttgggga tgtctccaga tcgtacttaa gttccttaag tgcttgtaat atcctgtctt
     1201 ttttatagca tagctcgcct gtttttgtgt ctaccagtcc tgcttcgatc agtagcccag
     1261 ccgcgagaaa gagccggtga ttgcgttccc gtcgttgttt ttttccctcg attcgttttt
     1321 ggaccgcctg ttttcgttcg ctctcctgtg cctgtaagtc gtttttttcc gctgttgcca
     1381 gtacgtggag aagtttgcgt tcctgttctg tgagtccggc ttcgttgtcg atctggtcaa
     1441 taatctgtct ctgtcgtgcg gtctgttctc cgagcttcct gatgaatccg atccgtttct
     1501 tcagccattc gatgttgctg atgcgcatgt tcgtgatgct ccctggtgtt tttttgcggt
     1561 gtgttgtggt cgtgcagtat atcgcttgcg gtcggcgggt gcagggggta tcttccgtgc
     1621 taattcgtaa aattagaagg aagcgcactt atatgtttct ctccttcgtc gagaaacagt
     1681 gcgctcctgc agagggctga aaccgtggca acctatcacc tcagcgttaa atttggtggc
     1741 aaggggcagg ctgcaaacca cgccgattac atcgagcgca aagagaaata ccgcgaccgt
     1801 caggatttgg aatattccgc acacggaaac atgccggaat gggccaggga taatccttcc
     1861 catttctggc aggcggctga ccagtttgaa cgtgcgaacg gttcgaccta tagggaactt
     1921 gaaattgcat taccccgtga actgacaccg gaacagcgcc ttgaactggt gcaggatttt
     1981 gtacgtcagg aagctggcga acggcacgcc tggagttttg ccattcacaa tccgaaagcg
     2041 agcattgacg gcggcgaaca gcctcatgcg catatcatga tgagtcagcg tgtgaatgat
     2101 ggcattgaac gtacaccgga acagtatttc cggcgctata acgccaggta tcccgagcgg
     2161 ggaggtgcga aaaaggattc cggaagcctg actctcactc agcaaaaaga gcagttgcgg
     2221 gaattgcgaa aacgctggga ggtgaagcac aacgaacata tgagaaagca tggctttgaa
     2281 aggggcttca ttgactgccg gacgctgaaa gaacagggca ttgaacgacg tcccgaagtg
     2341 catctgggtt ttgaagctgc cggacggctt gatgatgccc agcgtcatga catcatgcaa
     2401 aaacggtctg aaccggacta ttcccgccat cttgaacagg cgcgaacggt cgggcatgac
     2461 atcagggaat ggctggacac tccggttaag caacatgaac tgatcagaag gctgcaacat
     2521 gatgttgcgc gggagcatgg ctttgacatg agagacagag tgagcagaca gaaccgaact
     2581 gcacctgaac gggttaaatc accgtcacgt ggattttcta tgggattctg aacatggcca
     2641 gcacagaaga aaaattactc acgctcctgt tatcggtgga gcatctgcaa cataccgcaa
     2701 tggagcaaca gaaagctgtt gctgattctc tggctgtggc aagggaaact cttcgcggat
     2761 atcgggatat ttctgacggt gtggcaaaac aggtcaggga tgaagtcaga aatgaggtgc
     2821 agaagctgga cgtggcagga ctgataggtg agcgcatcag ccaggatttt cagcggctgg
     2881 aagagtcagt cgcaaacatg aaggcaaacg cggatgccct gaacagggat attgcgacaa
     2941 ttcacagcaa tatcgtgcag gaatacaaca cccttaaagg gtatagctgg aaatggctgg
     3001 gtggggtgtc agtggctttt ctggttatgt tctgtgtgct cggatgggga atgaaaacaa
     3061 tactcgacag gaactacaac gccacagcgg cggtgtatca gaaagttgtg acactggaag
     3121 ataaactggt ggagaaacct gtcacgggga agagccggaa aaactgacgc gcttcgcttg
     3181 tccaacgcct cacgacccgc gaaaggattt cgcgaaccgt tcaccgtttt tttcaatatg
     3241 attctgtctg ctttggtcga taaagcagac gatagtattc cagccttcgt atatataacg
     3301 gtacgtgctc atcagtaaga gctggcggta ttgtgttatc agggggaagt tttttcagat
     3361 actcacgaaa tgcctggctt gatgccataa aatctacagt agcatcgata atatgtgggg
     3421 tgttttcacc taattttgcc atgatgataa tccttatatt atcacggttt gtgaatgata
     3481 gggtgtcact attatttatg cgttagtgaa ccagaaaaaa tcccccgttg gcataggggg
     3541 atatgtaagg agaaataact tcgcaggtgt tcttgtttgt ataacgtgaa aagactgcac
     3601 gcaacttaat aagtctgccg tacttacttt cagttttcaa cataaggttt ataacaatgt
     3661 atggaatgga agtgagcttt aaggtttcag ctctaaaaaa taaaatattt attagttggc
     3721 tggttgttgt tcttgttctg tatgtatata aacactatcg acatattatg accgggaaca
     3781 ttccttaccc cctctgcgct cgcagaggtt ttttttgtca ttaattaata atgaactgaa
     3841 atgcagttcc gatacctcca tttgcacatt atccctgaca tatggtaccg gaaccgcatt
     3901 aattttatgt tataagagtt tatttttaat gtccagatgg tttaatagta tttatatgta
     3961 attagctgta tggatagtac tggttttatt gtataacttg tcgttttttt tttattgttt
     4021 ttgtgtggtg tgctaatctt taagttataa aaaaacccct tggaatacag gggcgaaagt
     4081 aaggaaaaca aatggatgct cagtttgtgt gacactttaa actaatggaa ctatagagtg
     4141 gaggttgtta cagtcgcttt tataaaacag caaccagttt ttttcaatag tctgatgatg
     4201 gttgtgacag ttttgtgata ttgttcaaaa tgtttcttgt tgaatgtttg ttttttggtc
     4261 tattggtgtc tggttttatt ttttctgcta ttacatatca agaaatgtga ttggtgtagc
     4321 aagagtggaa tgcgatgtgg ataaactaat tggttgtcga atctaattta cttagaaaac
     4381 tacacttgtt tttgtgtatg aatttagtga atttgtctgt ctatgtgttt tcgtgcattt
     4441 ataaatcctt cgtcaggatc ttaaatttag tgttgaatat gtagggcctg tacggtaatt
     4501 attatttagc gtagctactt catcgttgtt cgaacacaac caacaacctg taattgttgt
     4561 ttctcttggt tccccacttt tgtggggttt tttatctctt ccgtgcatcg taaagcgcgg
     4621 actccttctg gaaaggttgt tttagggcat cactgaggca ttttttgcgt ttgtgaatat
     4681 tgagcatccg ataactaaaa tatttcccat agactctgat tcttgccacc gaccctaggt
     4741 cggttttttt tgtcctggcg agatgtgaag tttggaaaca ggaaaccccg tcagagcgcc
     4801 acgcaccagg ggagaaaact tgtcgttttg agccgttcgg tgttcagaac gcacgaaacc
     4861 gatcgcgcat cgctttcgtg catagttatg caacgcgcta aaaacgattc tgacgcattt
     4921 tttatgttta ccctggtgtt tccttgtctt tttgtgtttt ttgcgtcaga aagcgactga
     4981 gggcgtttta aggggtacgt acaacgggag ttatggtaaa tggatcgggt tttcgggaag
     5041 gttcgacagg atttgccgtt gggtgtagtg taagcgactg aaaaacaaac gcccctgaaa
     5101 ttcgtgtcta tccgccaaga ctttcacgaa aatacagggg cgatgtcggt cgataacctg
     5161 gaaggaatcg taacac
//
