LOCUS       NZ_CP101278             6648 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-014 plasmid pEC21Z014-7K, complete
            sequence.
ACCESSION   NZ_CP101278
VERSION     NZ_CP101278.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593405
            Assembly: GCF_024298565.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:14:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,046
            Genes (coding)                    :: 4,836
            CDSs (with protein)               :: 4,836
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 210
            CDSs (without protein)            :: 210
            Pseudo Genes (ambiguous residues) :: 1 of 210
            Pseudo Genes (frameshifted)       :: 78 of 210
            Pseudo Genes (incomplete)         :: 126 of 210
            Pseudo Genes (internal stop)      :: 45 of 210
            Pseudo Genes (multiple problems)  :: 39 of 210
            Pseudo Genes (short protein)      :: 3 of 210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101278.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-014"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z014-7K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-01-25"
     gene            join(6572..6648,1..1492)
                     /gene="cea"
                     /locus_tag="NML17_RS25710"
                     /old_locus_tag="NML17_25715"
     CDS             join(6572..6648,1..1492)
                     /gene="cea"
                     /locus_tag="NML17_RS25710"
                     /old_locus_tag="NML17_25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1878..2015
                     /locus_tag="NML17_RS25715"
                     /old_locus_tag="NML17_25720"
     CDS             1878..2015
                     /locus_tag="NML17_RS25715"
                     /old_locus_tag="NML17_25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            2576..2890
                     /locus_tag="NML17_RS25720"
                     /old_locus_tag="NML17_25725"
     CDS             2576..2890
                     /locus_tag="NML17_RS25720"
                     /old_locus_tag="NML17_25725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224157910.1"
                     /translation="MSVDFCDARQGGGAYGKNASGAAFFALCLFAHMFFPALSPDSVDN
                     RIAVFEQADSARRSRTTERSESVSEEAEKRLDVHFLLTHLCGISHPAWCTFHTMRPDAE
                     "
                     /name="hypothetical protein"
     gene            complement(3042..3233)
                     /locus_tag="NML17_RS25725"
                     /old_locus_tag="NML17_25730"
     CDS             complement(3042..3233)
                     /locus_tag="NML17_RS25725"
                     /old_locus_tag="NML17_25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165974.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3298..3621
                     /locus_tag="NML17_RS25730"
                     /old_locus_tag="NML17_25735"
     CDS             3298..3621
                     /locus_tag="NML17_RS25730"
                     /old_locus_tag="NML17_25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956000.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3830..>4261
                     /locus_tag="NML17_RS25735"
                     /old_locus_tag="NML17_25740"
                     /pseudo=""
     CDS             3830..>4261
                     /locus_tag="NML17_RS25735"
                     /old_locus_tag="NML17_25740"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4299..4817
                     /locus_tag="NML17_RS25740"
                     /old_locus_tag="NML17_25745"
     CDS             4299..4817
                     /locus_tag="NML17_RS25740"
                     /old_locus_tag="NML17_25745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            4824..5057
                     /locus_tag="NML17_RS25745"
                     /old_locus_tag="NML17_25750"
     CDS             4824..5057
                     /locus_tag="NML17_RS25745"
                     /old_locus_tag="NML17_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5375..5794)
                     /locus_tag="NML17_RS25750"
                     /old_locus_tag="NML17_25755"
     CDS             complement(5375..5794)
                     /locus_tag="NML17_RS25750"
                     /old_locus_tag="NML17_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(5875..6312)
                     /locus_tag="NML17_RS25755"
                     /old_locus_tag="NML17_25760"
     CDS             complement(5875..6312)
                     /locus_tag="NML17_RS25755"
                     /old_locus_tag="NML17_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          2878..2957
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.765"
                     /coverage="100.0"
                     /evalue="7.760000000000001e-33"
     Typing          complement(2301..2846)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.273"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3612..5161
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.756"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggtactcct gacgggagtg gctctggcgg cggaggtgga aaaggaggca gtaaaagtga
       61 aagttctgca gctattcatg caactgctaa atggtctact gctcaattaa agaaaacaca
      121 ggcagagcag gctgcccggg caaaagctgc agcggaagca caggcgaaag caaaggcaaa
      181 cagggatgcg ctgactcagc gcctgaagga tatcgtgaat gaggctcttc gtcacaatgc
      241 ctcacgtacg ccttcagcaa cagagcttgc tcatgctaat aatgcagcta tgcaggcgga
      301 agcagagcgt ttgcgccttg cgaaagcaga agaaaaagcc cgtaaagaag cggaagcagc
      361 agaaaaggct tttcaggaag cagaacaacg acgtaaagag attgaacggg agaaggctga
      421 aacagaacgc cagttgaaac tggctgaagc tgaagagaaa cgactggctg cattgagtga
      481 agaagctaaa gctgttgaga tcgcccaaaa aaaactttct gctgcacaat ctgaagtggt
      541 gaaaatggat ggagagatta agactctcaa ttctcgttta agctccagta tccatgcccg
      601 tgatgcagaa atgaaaacgc tcgctggaaa acgaaatgaa ctggctcagg catccgctaa
      661 atataaagaa ctggatgagc tggtcaaaaa actatcacca agagccaatg atccgcttca
      721 gaaccgtcct ttttttgaag caaccagacg acgggttggg gccggtaaga ttagagaaga
      781 aaaacaaaaa caggtaacag catcagaaac acgtattaac cggataaatg ctgatataac
      841 tcagatccag aaggctattt ctcaggtcag taataatcgt aatgccggta tcgctcgtgt
      901 tcatgaagct gaagaaaatt tgaaaaaagc acagaataat ctccttaatt cacagattaa
      961 ggatgctgtt gatgcaacag ttagctttta tcaaacgctg actgaaaaat atggtgaaaa
     1021 atattcgaaa atggcacagg aacttgctga taagtctaaa ggtaagaaaa tcggcaatgt
     1081 gaatgaagct ctcgctgctt ttgaaaaata caaggatgtt ttaaataaga aattcagcaa
     1141 agccgatcgt gatgctattt ttaatgcgtt ggcatcggtg aagtatgatg actgggctaa
     1201 acatttagat cagtttgcca agtacttgaa gattacgggg catgtttctt ttggatatga
     1261 tgtggtatct gatatcctaa aaattaagga tacaggtgac tggaagccac tatttcttac
     1321 attagagaag aaagctgcag atgcaggggt gagttatgtt gttgctttac tttttagctt
     1381 gcttgctgga actacattag gtatttgggg tattgctatt gttacaggaa ttctatgctc
     1441 ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt aattattctt
     1501 tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac aaagtagatt
     1561 atatagcata aataggttta attttgctac gggggcgtta tttaggtttt ttcttctttc
     1621 gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt ttgaatatgg
     1681 acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa ggaaataata
     1741 cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca ggccaaataa
     1801 aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata ttcttttcat
     1861 tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg ataaacctcc
     1921 ttgttggatg tcaggctaac tatatacgtg atgttcaggg agggaccatc gcaccatcct
     1981 cctcttctaa actgacgggg atcgcggttc agtagaaaag atcaaaggat cttcttgaga
     2041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccaacggt
     2101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2161 agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact acttcaagaa
     2221 ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt ggctgctgcc
     2281 agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc ggataaggcg
     2341 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2401 accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2461 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2521 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2581 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaaacg ccagcggcgc
     2641 ggcttttttt gccctttgtc tttttgctca catgttcttt ccggccttat cccctgattc
     2701 tgtggataac cgtattgccg tttttgagca agcagactcc gctcgccgca gtcgaacgac
     2761 cgagcgcagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc attttctcct
     2821 tacgcatctg tgcggcattt cacacccggc atggtgcact tttcatacaa tgcgccctga
     2881 tgccgaataa gtaagccagt atacactccg ctatcgctac gtgactgggt aagggctgcg
     2941 ccccgacacc cgctaaaacc cgctgacgcg tcctgacggg cttgtcggct cccggcatcc
     3001 gctcacagac aagctgtgac cgtttccggg agctgcatgt gtcagaggtt ttcaccgtca
     3061 tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg aagcgattca
     3121 cagatatctg cctgttcatc tgcgtccagt tcgttaagtt tctccagcag cgttaatgtc
     3181 tggcctctga tgaaacgggc catgttaagg gcggtttttt cctgtttagt cactgatgcc
     3241 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg cgagaggatg
     3301 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgctg tgagggtaaa
     3361 caactggcgg tatggatgcg gcgggtctgc ctgggtgagc cggttgcccg ttccgggaag
     3421 ctgccgacac tggcaccgcc gttactgcgt cagctggccg ccatcgggaa taacctgaat
     3481 cagacagcga ggaaggtgaa cagcggacag tggtcttccg gtgaccgggt tcaggtggtg
     3541 gccgcactga tggccatcgg ggatgaactg cgccggctgc gtctggccgt cagggaacag
     3601 gggacgcggg atgatagtta aatttcatgc caggggaaaa ggtggtggca gtggtccggt
     3661 tgattacctg ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc agggtaatcc
     3721 ggaagaagtc cgggaactca tcgatgccac gccatttgcg aagaaataca cgtcaggtgt
     3781 tctgtcgttc gcggagaagg agctgccgcc gggaggacgt gaaaaagtga tggcgagctt
     3841 tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca
     3901 ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc tacagagcgg
     3961 aaaacgcctc cagccgtact acgaccgggc agacaggccg agaattgatg cctggcagac
     4021 gctggtgaat caccattacg ggctgcatga ccccaacgcc ccggagaacc gccggatact
     4081 gactctccct gataacctgc cgaaaacgaa acaggcgctt gcagagagcg ttacacgcgg
     4141 gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga ttcaggcgct
     4201 cacggaggca gggattgaag tggtcagggt gacgcgaagc agtatcagca ttgcagaccc
     4261 gaacggcggg aggaatatca ggctgaaagg agcattttat gagcaatctt ttacagacgg
     4321 gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaaca
     4381 acgagttcag gaggctcggc gagtctgtca gcgaggctgt gacctcaaac gagacgaaaa
     4441 tcagagacgc tatagccctg ttcacagcct cgacagagga atcgctggaa aaacaccggg
     4501 aaggggtgaa agaggcgatg atgcagcaca ggagggacgt gttaaagctg gcagggaata
     4561 cgggcatgat gttactgggg atagtctttc tcctgtttac cgcgagtggc gggacgctct
     4621 ggtatcttgg agggaggata caggcgaacc tggaagaaat caggaagcag gaagagacat
     4681 tgcagaaact gaacgcgaag acatggggcg tggagtttgt gcaggacggg aacaggaaat
     4741 tccttgtcct tccgtacggg aaatcagcgg aggtgattcc ctttcagggg aaagagtggg
     4801 tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg aacgccttag
     4861 agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat
     4921 tgcagaagat gttttcgctt acgcaacggg acaacgcgat gctgaacgag cgggtcatgc
     4981 agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc cagttataca
     5041 gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg tgttatccct
     5101 ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggccctt cgctgggatg
     5161 gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc
     5221 tctggttgca taggtattca tacggttaaa atttatcagg cgcgatcgcg gcagtttttc
     5281 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc
     5341 ggtgcgtaca attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga
     5401 gataaaccgc agaaatcgtg tcagccagca gcctggattt tttcggggta aggtctttgc
     5461 tgcggtaatc agtacccgca aattgagttg tgccccggag tgagtttaaa aattcctggc
     5521 tgtgaaactg ctgaatgcgt tgttgcacat cactccgtga ttttcctgtc gctttatctt
     5581 tagctcccat atcccagaac tgccgaaaaa aaggcaccat cattcgtgtt gtgtcggctt
     5641 tttgggtgga aaagttggga tcaaaaccgt catcagaagc atatacgtaa tgccgggtat
     5701 gttgttccac gctgcccttt ggggtacagg ctgtgagagc tactgttgct gttatcatgg
     5761 cgataagaca aagtttgttt ccggtacgtc tcatgagtgt ctccctacct taaagtattt
     5821 attctcggtg agttattatc cgaagactat tttatcatca gtctctgaca gcgttcagcg
     5881 accgaacaac ctggaccaga atccccgttt cttttccagt tccagtgcct gcctgagctg
     5941 ggctatctca ttttgtagct gttcccgttc tgcttcctgg cgtctgcgat ccatatcctg
     6001 tgcctgttta tcctcaagca tcagcgtcag gcattgtttc agctcattca gctcccggag
     6061 aatgcgttct gtctgctgat catgtggatt ttctgcatgt ccctcactgt gcctttctgg
     6121 tgtctcattc tgctttaatt cgccgtatgc ccggatcagt tcactggttt caaactcccg
     6181 tcgtccgtcc ctgccggtac ggtaactgac aaggcctgcg cgcatatcac ggtaaaactg
     6241 tgaacgcgat ctgcctgtca tttttagtgc gtcccggagt gtgtgccatg ccataaagtg
     6301 acagtgtccc atagatgtct catctcatag tttcagtaaa acataatgag gtctgagaac
     6361 ggtaatgttt gtgctggttt ttgtggcatc gggcgagaat agcgcgtggt gtgaaagact
     6421 gtttttttga tcgttttcac aaaaatggaa gtccacagtc ttgacaggga aaatgcagcg
     6481 gcgtagcttt tatgctgtat ataaaaccag tggttatatg tacagtattt atttttaact
     6541 tattgtttta aaagtcaaag aggattttat aatggaaacc gcggtagcgt actataaaga
     6601 tggtgttcct tatgatgata agggacaggt aattattact cttttgaa
//
