LOCUS       NZ_CP101262             4060 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-078 plasmid pEC21Z078-4K, complete
            sequence.
ACCESSION   NZ_CP101262
VERSION     NZ_CP101262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593408
            Assembly: GCF_024298705.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:23:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,970
            CDSs (total)                      :: 4,844
            Genes (coding)                    :: 4,574
            CDSs (with protein)               :: 4,574
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 71 of 270
            Pseudo Genes (incomplete)         :: 187 of 270
            Pseudo Genes (internal stop)      :: 47 of 270
            Pseudo Genes (multiple problems)  :: 35 of 270
            Pseudo Genes (short protein)      :: 4 of 270
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101262.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-078"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z078-4K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-03-29"
     gene            complement(join(3411..4060,1..52))
                     /locus_tag="NML20_RS24755"
                     /old_locus_tag="NML20_24760"
     CDS             complement(join(3411..4060,1..52))
                     /locus_tag="NML20_RS24755"
                     /old_locus_tag="NML20_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(585..839)
                     /gene="mobC"
                     /locus_tag="NML20_RS24760"
                     /old_locus_tag="NML20_24765"
     CDS             complement(585..839)
                     /gene="mobC"
                     /locus_tag="NML20_RS24760"
                     /old_locus_tag="NML20_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1018..3165
                     /gene="mobQ"
                     /locus_tag="NML20_RS24765"
                     /old_locus_tag="NML20_24770"
     CDS             1018..3165
                     /gene="mobQ"
                     /locus_tag="NML20_RS24765"
                     /old_locus_tag="NML20_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_171874286.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGKSGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     Typing          complement(841..1017)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1018..1376
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3412..4060)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          932..3162
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.39"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtagcttgc ctcgataagg gcgtttgatt tgtaagctat cagatctgac atttcgcttg
       61 ctcgttgcta ttggtggcgt ttttttactg tacgccactt atcatatggg tggaagtttt
      121 ttttttgcaa cccccttttt ctgtggatta cacccccaaa agctccacct ttcaccccca
      181 aaagctccac ctttcacccc caaaagctcc acctttcacc cccaaaagct ccacttatct
      241 tgccagaaca tctttaaaat catggtgtta caagacctaa aaaggtttta aaaaaaagat
      301 ctaaaaataa gatcttaaaa acttaacggc gagcctgtgg ataacttggg gtgctgaaaa
      361 caaaaaacca ataaaaacaa tgcgttgcaa taaagcagaa ttgcaagttt cgaatacacc
      421 ccctccaacc ctgtggaaac ccagtattca tgcgggttca gcgcatatca cctgccgaaa
      481 aaccgtgcgt atgtgctcaa cctggcgact gattcacttc cgcttcctcg cccactcgct
      541 cgctaacgct cggtcgctgg ggctgcggcg agcggacgat acaatcactg ttctggcagc
      601 gcctccagct cgttaatcag cggcagtatg cgcgtcacat cagcctgtcg tgtcacctct
      661 gccttcagga actggagtaa ccaggcacgc aactgcacct cctccggctt tctgaccttt
      721 gccagcaatg cagcacccac aacaaccttc tgcgctgtgt ccaggctccg gctcttcgcc
      781 ttcaggcgct gtaatctcgc ctcagcatca gcaatcttct gttcgagcgt tctgctcatg
      841 atgtaactcc gtgcgcgatg aaaaatcgca ttttagcgcg tcactggtag tttaaaaact
      901 gagctggcat aatgcacggc aaatcacgaa gtgcgcactt atacaatctc cactccgttt
      961 cgattgtgtg cgccctgcgg ggctgaaaga aaactacaaa aaggcattac ggcagaaatg
     1021 gcgatttatc atctcagcat gaaaattatc tccagaagca gcggctacag cgccgtagct
     1081 tccgctgcct atcgttccgg ttcactgatg cttgatgagc gtaccggact gactcacgac
     1141 tacacccgca aaagcggtgt ggctgaggct gtgattctga cccccgccac cgcccctgcc
     1201 tggtgtacga accgtgccga actctggaat gccgttgaga aggccgaacg ccggaagaac
     1261 tcacaacttg cgcgggaaat tgaactcgcc atcccccacg aactgccgca ggatgccgca
     1321 cgggaaaccg ttctcgcctt tgtcagagaa aactttgtca gtcagggcat gattgctgat
     1381 gtggcgttcc atcacatgga taaaacaaat ccccatgcgc acatcatgct gaccacgagg
     1441 gctatcggac ctgctgggtt cagcggaaag gtcagggact ggaacgaccg gacacatgcc
     1501 gagacgtggc gggcatcatg ggctgaccat gcaaacaggg cactggcgaa cgccggttat
     1561 caggaagaga tagaccatcg ttcatacgag cgacaggggc ttgagaaaac gcctggtatt
     1621 cacctcggaa agtctgcctg tgcgatggaa acacgcggta tcgaaacaga acgcggcgaa
     1681 cagaatcgtc tgattaacag gcttaacctg gaaatacaga tttcccgcac cgaactcagg
     1741 aacggagggc tgacagagcc tgcccgccgt gtggctgacc ttctgaatat cgggcttcct
     1801 gacaacgcaa cttcgtacac actacgtgac attatcgaag cgttgccgaa ggacagtaac
     1861 tcggcatgga aactcaccag taatgccatg tcgatgatgg cagatatgca ggcgacgcgc
     1921 agacgctggg aagagctgaa caccgaacga aaggaagcga tgagccatgc tgccagcctg
     1981 aaaaaatcat cgccgttttc gtccggtttt tcccgtattc ccctgatgca atgggcggca
     2041 ccggaatacc gtagtgagca gggaaaaatc cagtctctga gccagcaaat ggaaaaatta
     2101 cgacagcatt accggaaggt ggagaaacag gacattcccg cgagtcagat ggcatttgaa
     2161 aaacagtggc agcagtgggg gaaatccggc cttgcagctc tcagagccca gttgcttaaa
     2221 cgtgaagagg aattgcgccg gaaggaacag gaagatactg aacgtagtcg cgcggagcag
     2281 ctcaggaaaa atgacaatgc cgttatggat tcaggggtgc tgagagcagt cgttacgggg
     2341 tatggcgagg ctcccatgcc tggtaacggg gaaatgacct gttaccttct gcttcacaac
     2401 cggaacggag aatatacgct ctgggggaac gagctggaga aataccgcac cagaatcttt
     2461 gagagtgttg atttaatgcg tgacaggagt ggttatatct gtgaccgaag cgaaatcggc
     2521 caacgtccgc cactgcaacg tgtttattcc tctgcaacct ttgagcaact gctgacgcag
     2581 gtttgccaga catggccgca acatacccgc aatctccgcc agccgaagac atggccggag
     2641 tctttctgtc ttggagaaga ccgccagcca gccatgccat caccagcggc aaggaaggtg
     2701 gattttatgc agggacgtct gccaccgacg ctgatgccgg tcatgagttc tgtcgaccgg
     2761 gaaacccgcc agcttcagct tttgctggtc atgggcgtgg atgactctct gggcggtgtt
     2821 atccggctta atggtacgct gtatcccgcg tttgccgttc cctcagccga taacaggcat
     2881 ctggtcatca gtgcgctgac cgataagggg ttacggtatg cagggtatgg tgttgccgta
     2941 aatcacgatg cagacagcca catatcacct gcaccagaac tgatgcagtt tcatctgaaa
     3001 acccgtgagg caccgctttt tgctgccgtc aacacaccag aaaaacagcc ggatcatctg
     3061 ttccgctcgc tgggttttaa tcgtacctgg gacgaatggc ggcgcgagga agatgcacga
     3121 acacacgcca ccgagaggag acatgatcgc ggttggtcac agtgaatgtg aacaatattt
     3181 tgttttgttt aacatcgtgt tatgttcttc gatgttcatc gacagggatg aatgcaagaa
     3241 gcacacacat aatgcaagca tagttttgtt tttccgcccc caaaaaatgg gggttctttt
     3301 ttcgctgcat tagagcgctt ttgacgcaat ttttcacttc aatagtacct tcgcactact
     3361 aacacaaaaa aaacgcagca gggagcgttt taggtgcctt aaaatcgatt ttagatcgtc
     3421 atcttgatct gtttgtcttc cttgaagcga aaatgcaggg ctacaaccgt gcgccccttt
     3481 ttgatcgtct caacagttac aaccagatcg cttttttcgt tcagttcggc gatggctggc
     3541 ttgattatca tcttattcaa gtctttgaat tgttgatatt tatcttcaac ggcaagcatg
     3601 gttcggaaat catcgatagc tatcgttctg tcaccggtgc tgcggaactg ctggagcagc
     3661 tcgtaaatgc ggatgctgta ggtgctggaa aggctggaga cgttcttcac gactacccgc
     3721 gtaaactgcc ccttgagctg tgtcagatac ggcatgatgg catcggagaa ggtgatatct
     3781 actttcgcct cacctttgaa atactgcgcc cggtactgca cccagcggaa ctcttctcgt
     3841 ttttcctcgt tcttgatgat tactgacctg tcccagaggc gatctatcgc ttccttgagc
     3901 ctagcttcag cgtgctgccg tcccatgtca ggaaaggcat catgaaactc tgcagccgtg
     3961 atggtcatgg ttttttgttc atccggcgaa ggggaatctt ctccggattt taggcggctg
     4021 atacagacca gcaggaagcg ctgttcctgc aaagtgagct
//
