LOCUS       NZ_CP101256             4060 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-083 plasmid pEC21Z083-4K, complete
            sequence.
ACCESSION   NZ_CP101256
VERSION     NZ_CP101256.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593409
            Assembly: GCF_024298545.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:49:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,961
            CDSs (total)                      :: 4,835
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 72 of 271
            Pseudo Genes (incomplete)         :: 187 of 271
            Pseudo Genes (internal stop)      :: 47 of 271
            Pseudo Genes (multiple problems)  :: 35 of 271
            Pseudo Genes (short protein)      :: 4 of 271
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101256.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-083"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z083-4K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-03-29"
     gene            complement(join(2109..4060,1..196))
                     /gene="mobQ"
                     /locus_tag="NML21_RS24710"
                     /old_locus_tag="NML21_24775"
     CDS             complement(join(2109..4060,1..196))
                     /gene="mobQ"
                     /locus_tag="NML21_RS24710"
                     /old_locus_tag="NML21_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_171874286.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGKSGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            375..629
                     /gene="mobC"
                     /locus_tag="NML21_RS24715"
                     /old_locus_tag="NML21_24780"
     CDS             375..629
                     /gene="mobC"
                     /locus_tag="NML21_RS24715"
                     /old_locus_tag="NML21_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1162..1863
                     /locus_tag="NML21_RS24720"
                     /old_locus_tag="NML21_24785"
     CDS             1162..1863
                     /locus_tag="NML21_RS24720"
                     /old_locus_tag="NML21_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(2..197)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.41e-98"
     Typing          198..374
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(3899..4060)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.71e-79"
     Typing          1163..1863
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2113..4058)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.325"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggttcgtaca ccaggcaggg gcggtggcgg gggtcagaat cacagcctca gccacaccgc
       61 ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc aagcatcagt gaaccggaac
      121 gataggcagc ggaagctacg gcgctgtagc cgctgcttct ggagataatt ttcatgctga
      181 gatgataaat cgccatttct gccgtaatgc ctttttgtag ttttctttca gccccgcagg
      241 gcgcacacaa tcgaaacgga gtggagattg tataagtgcg cacttcgtga tttgccgtgc
      301 attatgccag ctcagttttt aaactaccag tgacgcgcta aaatgcgatt tttcatcgcg
      361 cacggagtta catcatgagc agaacgctcg aacagaagat tgctgatgct gaggcgagat
      421 tacagcgcct gaaggcgaag agccggagcc tggacacagc gcagaaggtt gttgtgggtg
      481 ctgcattgct ggcaaaggtc agaaagccgg aggaggtgca gttgcgtgcc tggttactcc
      541 agttcctgaa ggcagaggtg acacgacagg ctgatgtgac gcgcatactg ccgctgatta
      601 acgagctgga ggcgctgcca gaacagtgat tgtatcgtcc gctcgccgca gccccagcga
      661 ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga atcagtcgcc aggttgagca
      721 catacgcacg gtttttcggc aggtgatatg cgctgaaccc gcatgaatac tgggtttcca
      781 cagggttgga gggggtgtat tcgaaacttg caattctgct ttattgcaac gcattgtttt
      841 tattggtttt ttgttttcag caccccaagt tatccacagg ctcgccgtta agtttttaag
      901 atcttatttt tagatctttt ttttaaaacc tttttaggtc ttgtaacacc atgattttaa
      961 agatgttctg gcaagataag tggagctttt gggggtgaaa ggtggagctt ttgggggtga
     1021 aaggtggagc ttttgggggt gaaaggtgga gcttttgggg gtgtaatcca cagaaaaagg
     1081 gggttgcaaa aaaaaaactt ccacccatat gataagtggc gtacagtaaa aaaacgccac
     1141 caatagcaac gagcaagcga aatgtcagat ctgatagctt acaaatcaaa cgcccttatc
     1201 gaggcaagct acaagctcac tttgcaggaa cagcgcttcc tgctggtctg tatcagccgc
     1261 ctaaaatccg gagaagattc cccttcgccg gatgaacaaa aaaccatgac catcacggct
     1321 gcagagtttc atgatgcctt tcctgacatg ggacggcagc acgctgaagc taggctcaag
     1381 gaagcgatag atcgcctctg ggacaggtca gtaatcatca agaacgagga aaaacgagaa
     1441 gagttccgct gggtgcagta ccgggcgcag tatttcaaag gtgaggcgaa agtagatatc
     1501 accttctccg atgccatcat gccgtatctg acacagctca aggggcagtt tacgcgggta
     1561 gtcgtgaaga acgtctccag cctttccagc acctacagca tccgcattta cgagctgctc
     1621 cagcagttcc gcagcaccgg tgacagaacg atagctatcg atgatttccg aaccatgctt
     1681 gccgttgaag ataaatatca acaattcaaa gacttgaata agatgataat caagccagcc
     1741 atcgccgaac tgaacgaaaa aagcgatctg gttgtaactg ttgagacgat caaaaagggg
     1801 cgcacggttg tagccctgca ttttcgcttc aaggaagaca aacagatcaa gatgacgatc
     1861 taaaatcgat tttaaggcac ctaaaacgct ccctgctgcg ttttttttgt gttagtagtg
     1921 cgaaggtact attgaagtga aaaattgcgt caaaagcgct ctaatgcagc gaaaaaagaa
     1981 cccccatttt ttgggggcgg aaaaacaaaa ctatgcttgc attatgtgtg tgcttcttgc
     2041 attcatccct gtcgatgaac atcgaagaac ataacacgat gttaaacaaa acaaaatatt
     2101 gttcacattc actgtgacca accgcgatca tgtctcctct cggtggcgtg tgttcgtgca
     2161 tcttcctcgc gccgccattc gtcccaggta cgattaaaac ccagcgagcg gaacagatga
     2221 tccggctgtt tttctggtgt gttgacggca gcaaaaagcg gtgcctcacg ggttttcaga
     2281 tgaaactgca tcagttctgg tgcaggtgat atgtggctgt ctgcatcgtg atttacggca
     2341 acaccatacc ctgcataccg taacccctta tcggtcagcg cactgatgac cagatgcctg
     2401 ttatcggctg agggaacggc aaacgcggga tacagcgtac cattaagccg gataacaccg
     2461 cccagagagt catccacgcc catgaccagc aaaagctgaa gctggcgggt ttcccggtcg
     2521 acagaactca tgaccggcat cagcgtcggt ggcagacgtc cctgcataaa atccaccttc
     2581 cttgccgctg gtgatggcat ggctggctgg cggtcttctc caagacagaa agactccggc
     2641 catgtcttcg gctggcggag attgcgggta tgttgcggcc atgtctggca aacctgcgtc
     2701 agcagttgct caaaggttgc agaggaataa acacgttgca gtggcggacg ttggccgatt
     2761 tcgcttcggt cacagatata accactcctg tcacgcatta aatcaacact ctcaaagatt
     2821 ctggtgcggt atttctccag ctcgttcccc cagagcgtat attctccgtt ccggttgtga
     2881 agcagaaggt aacaggtcat ttccccgtta ccaggcatgg gagcctcgcc ataccccgta
     2941 acgactgctc tcagcacccc tgaatccata acggcattgt catttttcct gagctgctcc
     3001 gcgcgactac gttcagtatc ttcctgttcc ttccggcgca attcctcttc acgtttaagc
     3061 aactgggctc tgagagctgc aaggccggat ttcccccact gctgccactg tttttcaaat
     3121 gccatctgac tcgcgggaat gtcctgtttc tccaccttcc ggtaatgctg tcgtaatttt
     3181 tccatttgct ggctcagaga ctggattttt ccctgctcac tacggtattc cggtgccgcc
     3241 cattgcatca ggggaatacg ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg
     3301 gcagcatggc tcatcgcttc ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc
     3361 gcctgcatat ctgccatcat cgacatggca ttactggtga gtttccatgc cgagttactg
     3421 tccttcggca acgcttcgat aatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc
     3481 ccgatattca gaaggtcagc cacacggcgg gcaggctctg tcagccctcc gttcctgagt
     3541 tcggtgcggg aaatctgtat ttccaggtta agcctgttaa tcagacgatt ctgttcgccg
     3601 cgttctgttt cgataccgcg tgtttccatc gcacaggcag actttccgag gtgaatacca
     3661 ggcgttttct caagcccctg tcgctcgtat gaacgatggt ctatctcttc ctgataaccg
     3721 gcgttcgcca gtgccctgtt tgcatggtca gcccatgatg cccgccacgt ctcggcatgt
     3781 gtccggtcgt tccagtccct gacctttccg ctgaacccag caggtccgat agccctcgtg
     3841 gtcagcatga tgtgcgcatg gggatttgtt ttatccatgt gatggaacgc cacatcagca
     3901 atcatgccct gactgacaaa gttttctctg acaaaggcga gaacggtttc ccgtgcggca
     3961 tcctgcggca gttcgtgggg gatggcgagt tcaatttccc gcgcaagttg tgagttcttc
     4021 cggcgttcgg ccttctcaac ggcattccag agttcggcac
//
