LOCUS       NZ_CP101250             4060 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-097 plasmid pEC21Z097-4K, complete
            sequence.
ACCESSION   NZ_CP101250
VERSION     NZ_CP101250.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593410
            Assembly: GCF_024298525.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:16:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,967
            CDSs (total)                      :: 4,841
            Genes (coding)                    :: 4,570
            CDSs (with protein)               :: 4,570
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 72 of 271
            Pseudo Genes (incomplete)         :: 187 of 271
            Pseudo Genes (internal stop)      :: 47 of 271
            Pseudo Genes (multiple problems)  :: 35 of 271
            Pseudo Genes (short protein)      :: 4 of 271
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101250.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-097"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z097-4K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-04-06"
     gene            join(2554..4060,1..641)
                     /gene="mobQ"
                     /locus_tag="NML22_RS24745"
                     /old_locus_tag="NML22_24710"
     CDS             join(2554..4060,1..641)
                     /gene="mobQ"
                     /locus_tag="NML22_RS24745"
                     /old_locus_tag="NML22_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_171874286.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGKSGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(887..1588)
                     /locus_tag="NML22_RS24750"
                     /old_locus_tag="NML22_24715"
     CDS             complement(887..1588)
                     /locus_tag="NML22_RS24750"
                     /old_locus_tag="NML22_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2121..2375)
                     /gene="mobC"
                     /locus_tag="NML22_RS24755"
                     /old_locus_tag="NML22_24720"
     CDS             complement(2121..2375)
                     /gene="mobC"
                     /locus_tag="NML22_RS24755"
                     /old_locus_tag="NML22_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2377..2553)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2554..2912
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(888..1588)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..638
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.226"
                     /coverage="99.0"
                     /evalue="1.1700000000000002e-132"
     Typing          2468..4059
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.636"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtccgccact gcaacgtgtt tattcctctg caacctttga gcaactgctg acgcaggttt
       61 gccagacatg gccgcaacat acccgcaatc tccgccagcc gaagacatgg ccggagtctt
      121 tctgtcttgg agaagaccgc cagccagcca tgccatcacc agcggcaagg aaggtggatt
      181 ttatgcaggg acgtctgcca ccgacgctga tgccggtcat gagttctgtc gaccgggaaa
      241 cccgccagct tcagcttttg ctggtcatgg gcgtggatga ctctctgggc ggtgttatcc
      301 ggcttaatgg tacgctgtat cccgcgtttg ccgttccctc agccgataac aggcatctgg
      361 tcatcagtgc gctgaccgat aaggggttac ggtatgcagg gtatggtgtt gccgtaaatc
      421 acgatgcaga cagccacata tcacctgcac cagaactgat gcagtttcat ctgaaaaccc
      481 gtgaggcacc gctttttgct gccgtcaaca caccagaaaa acagccggat catctgttcc
      541 gctcgctggg ttttaatcgt acctgggacg aatggcggcg cgaggaagat gcacgaacac
      601 acgccaccga gaggagacat gatcgcggtt ggtcacagtg aatgtgaaca atattttgtt
      661 ttgtttaaca tcgtgttatg ttcttcgatg ttcatcgaca gggatgaatg caagaagcac
      721 acacataatg caagcatagt tttgtttttc cgcccccaaa aaatgggggt tcttttttcg
      781 ctgcattaga gcgcttttga cgcaattttt cacttcaata gtaccttcgc actactaaca
      841 caaaaaaaac gcagcaggga gcgttttagg tgccttaaaa tcgattttag atcgtcatct
      901 tgatctgttt gtcttccttg aagcgaaaat gcagggctac aaccgtgcgc ccctttttga
      961 tcgtctcaac agttacaacc agatcgcttt tttcgttcag ttcggcgatg gctggcttga
     1021 ttatcatctt attcaagtct ttgaattgtt gatatttatc ttcaacggca agcatggttc
     1081 ggaaatcatc gatagctatc gttctgtcac cggtgctgcg gaactgctgg agcagctcgt
     1141 aaatgcggat gctgtaggtg ctggaaaggc tggagacgtt cttcacgact acccgcgtaa
     1201 actgcccctt gagctgtgtc agatacggca tgatggcatc ggagaaggtg atatctactt
     1261 tcgcctcacc tttgaaatac tgcgcccggt actgcaccca gcggaactct tctcgttttt
     1321 cctcgttctt gatgattact gacctgtccc agaggcgatc tatcgcttcc ttgagcctag
     1381 cttcagcgtg ctgccgtccc atgtcaggaa aggcatcatg aaactctgca gccgtgatgg
     1441 tcatggtttt ttgttcatcc ggcgaagggg aatcttctcc ggattttagg cggctgatac
     1501 agaccagcag gaagcgctgt tcctgcaaag tgagcttgta gcttgcctcg ataagggcgt
     1561 ttgatttgta agctatcaga tctgacattt cgcttgctcg ttgctattgg tggcgttttt
     1621 ttactgtacg ccacttatca tatgggtgga agtttttttt ttgcaacccc ctttttctgt
     1681 ggattacacc cccaaaagct ccacctttca cccccaaaag ctccaccttt cacccccaaa
     1741 agctccacct ttcaccccca aaagctccac ttatcttgcc agaacatctt taaaatcatg
     1801 gtgttacaag acctaaaaag gttttaaaaa aaagatctaa aaataagatc ttaaaaactt
     1861 aacggcgagc ctgtggataa cttggggtgc tgaaaacaaa aaaccaataa aaacaatgcg
     1921 ttgcaataaa gcagaattgc aagtttcgaa tacaccccct ccaaccctgt ggaaacccag
     1981 tattcatgcg ggttcagcgc atatcacctg ccgaaaaacc gtgcgtatgt gctcaacctg
     2041 gcgactgatt cacttccgct tcctcgccca ctcgctcgct aacgctcggt cgctggggct
     2101 gcggcgagcg gacgatacaa tcactgttct ggcagcgcct ccagctcgtt aatcagcggc
     2161 agtatgcgcg tcacatcagc ctgtcgtgtc acctctgcct tcaggaactg gagtaaccag
     2221 gcacgcaact gcacctcctc cggctttctg acctttgcca gcaatgcagc acccacaaca
     2281 accttctgcg ctgtgtccag gctccggctc ttcgccttca ggcgctgtaa tctcgcctca
     2341 gcatcagcaa tcttctgttc gagcgttctg ctcatgatgt aactccgtgc gcgatgaaaa
     2401 atcgcatttt agcgcgtcac tggtagttta aaaactgagc tggcataatg cacggcaaat
     2461 cacgaagtgc gcacttatac aatctccact ccgtttcgat tgtgtgcgcc ctgcggggct
     2521 gaaagaaaac tacaaaaagg cattacggca gaaatggcga tttatcatct cagcatgaaa
     2581 attatctcca gaagcagcgg ctacagcgcc gtagcttccg ctgcctatcg ttccggttca
     2641 ctgatgcttg atgagcgtac cggactgact cacgactaca cccgcaaaag cggtgtggct
     2701 gaggctgtga ttctgacccc cgccaccgcc cctgcctggt gtacgaaccg tgccgaactc
     2761 tggaatgccg ttgagaaggc cgaacgccgg aagaactcac aacttgcgcg ggaaattgaa
     2821 ctcgccatcc cccacgaact gccgcaggat gccgcacggg aaaccgttct cgcctttgtc
     2881 agagaaaact ttgtcagtca gggcatgatt gctgatgtgg cgttccatca catggataaa
     2941 acaaatcccc atgcgcacat catgctgacc acgagggcta tcggacctgc tgggttcagc
     3001 ggaaaggtca gggactggaa cgaccggaca catgccgaga cgtggcgggc atcatgggct
     3061 gaccatgcaa acagggcact ggcgaacgcc ggttatcagg aagagataga ccatcgttca
     3121 tacgagcgac aggggcttga gaaaacgcct ggtattcacc tcggaaagtc tgcctgtgcg
     3181 atggaaacac gcggtatcga aacagaacgc ggcgaacaga atcgtctgat taacaggctt
     3241 aacctggaaa tacagatttc ccgcaccgaa ctcaggaacg gagggctgac agagcctgcc
     3301 cgccgtgtgg ctgaccttct gaatatcggg cttcctgaca acgcaacttc gtacacacta
     3361 cgtgacatta tcgaagcgtt gccgaaggac agtaactcgg catggaaact caccagtaat
     3421 gccatgtcga tgatggcaga tatgcaggcg acgcgcagac gctgggaaga gctgaacacc
     3481 gaacgaaagg aagcgatgag ccatgctgcc agcctgaaaa aatcatcgcc gttttcgtcc
     3541 ggtttttccc gtattcccct gatgcaatgg gcggcaccgg aataccgtag tgagcaggga
     3601 aaaatccagt ctctgagcca gcaaatggaa aaattacgac agcattaccg gaaggtggag
     3661 aaacaggaca ttcccgcgag tcagatggca tttgaaaaac agtggcagca gtgggggaaa
     3721 tccggccttg cagctctcag agcccagttg cttaaacgtg aagaggaatt gcgccggaag
     3781 gaacaggaag atactgaacg tagtcgcgcg gagcagctca ggaaaaatga caatgccgtt
     3841 atggattcag gggtgctgag agcagtcgtt acggggtatg gcgaggctcc catgcctggt
     3901 aacggggaaa tgacctgtta ccttctgctt cacaaccgga acggagaata tacgctctgg
     3961 gggaacgagc tggagaaata ccgcaccaga atctttgaga gtgttgattt aatgcgtgac
     4021 aggagtggtt atatctgtga ccgaagcgaa atcggccaac
//
