LOCUS       NZ_CP101244             4060 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-101 plasmid pEC21Z101-4K, complete
            sequence.
ACCESSION   NZ_CP101244
VERSION     NZ_CP101244.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593411
            Assembly: GCF_024298505.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:17:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,810
            CDSs (total)                      :: 4,684
            Genes (coding)                    :: 4,453
            CDSs (with protein)               :: 4,453
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 59 of 231
            Pseudo Genes (incomplete)         :: 154 of 231
            Pseudo Genes (internal stop)      :: 45 of 231
            Pseudo Genes (multiple problems)  :: 27 of 231
            Pseudo Genes (short protein)      :: 4 of 231
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101244.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-101"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z101-4K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-04-13"
     gene            join(3004..4060,1..1091)
                     /gene="mobQ"
                     /locus_tag="NML23_RS23960"
                     /old_locus_tag="NML23_23955"
     CDS             join(3004..4060,1..1091)
                     /gene="mobQ"
                     /locus_tag="NML23_RS23960"
                     /old_locus_tag="NML23_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_171874286.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGKSGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(1337..2038)
                     /locus_tag="NML23_RS23965"
                     /old_locus_tag="NML23_23960"
     CDS             complement(1337..2038)
                     /locus_tag="NML23_RS23965"
                     /old_locus_tag="NML23_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(2571..2825)
                     /gene="mobC"
                     /locus_tag="NML23_RS23970"
                     /old_locus_tag="NML23_23965"
     CDS             complement(2571..2825)
                     /gene="mobC"
                     /locus_tag="NML23_RS23970"
                     /old_locus_tag="NML23_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2827..3003)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3004..3362
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1338..2038)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..1088
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.082"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2918..4059
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.638"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctctgagcca gcaaatggaa aaattacgac agcattaccg gaaggtggag aaacaggaca
       61 ttcccgcgag tcagatggca tttgaaaaac agtggcagca gtgggggaaa tccggccttg
      121 cagctctcag agcccagttg cttaaacgtg aagaggaatt gcgccggaag gaacaggaag
      181 atactgaacg tagtcgcgcg gagcagctca ggaaaaatga caatgccgtt atggattcag
      241 gggtgctgag agcagtcgtt acggggtatg gcgaggctcc catgcctggt aacggggaaa
      301 tgacctgtta ccttctgctt cacaaccgga acggagaata tacgctctgg gggaacgagc
      361 tggagaaata ccgcaccaga atctttgaga gtgttgattt aatgcgtgac aggagtggtt
      421 atatctgtga ccgaagcgaa atcggccaac gtccgccact gcaacgtgtt tattcctctg
      481 caacctttga gcaactgctg acgcaggttt gccagacatg gccgcaacat acccgcaatc
      541 tccgccagcc gaagacatgg ccggagtctt tctgtcttgg agaagaccgc cagccagcca
      601 tgccatcacc agcggcaagg aaggtggatt ttatgcaggg acgtctgcca ccgacgctga
      661 tgccggtcat gagttctgtc gaccgggaaa cccgccagct tcagcttttg ctggtcatgg
      721 gcgtggatga ctctctgggc ggtgttatcc ggcttaatgg tacgctgtat cccgcgtttg
      781 ccgttccctc agccgataac aggcatctgg tcatcagtgc gctgaccgat aaggggttac
      841 ggtatgcagg gtatggtgtt gccgtaaatc acgatgcaga cagccacata tcacctgcac
      901 cagaactgat gcagtttcat ctgaaaaccc gtgaggcacc gctttttgct gccgtcaaca
      961 caccagaaaa acagccggat catctgttcc gctcgctggg ttttaatcgt acctgggacg
     1021 aatggcggcg cgaggaagat gcacgaacac acgccaccga gaggagacat gatcgcggtt
     1081 ggtcacagtg aatgtgaaca atattttgtt ttgtttaaca tcgtgttatg ttcttcgatg
     1141 ttcatcgaca gggatgaatg caagaagcac acacataatg caagcatagt tttgtttttc
     1201 cgcccccaaa aaatgggggt tcttttttcg ctgcattaga gcgcttttga cgcaattttt
     1261 cacttcaata gtaccttcgc actactaaca caaaaaaaac gcagcaggga gcgttttagg
     1321 tgccttaaaa tcgattttag atcgtcatct tgatctgttt gtcttccttg aagcgaaaat
     1381 gcagggctac aaccgtgcgc ccctttttga tcgtctcaac agttacaacc agatcgcttt
     1441 tttcgttcag ttcggcgatg gctggcttga ttatcatctt attcaagtct ttgaattgtt
     1501 gatatttatc ttcaacggca agcatggttc ggaaatcatc gatagctatc gttctgtcac
     1561 cggtgctgcg gaactgctgg agcagctcgt aaatgcggat gctgtaggtg ctggaaaggc
     1621 tggagacgtt cttcacgact acccgcgtaa actgcccctt gagctgtgtc agatacggca
     1681 tgatggcatc ggagaaggtg atatctactt tcgcctcacc tttgaaatac tgcgcccggt
     1741 actgcaccca gcggaactct tctcgttttt cctcgttctt gatgattact gacctgtccc
     1801 agaggcgatc tatcgcttcc ttgagcctag cttcagcgtg ctgccgtccc atgtcaggaa
     1861 aggcatcatg aaactctgca gccgtgatgg tcatggtttt ttgttcatcc ggcgaagggg
     1921 aatcttctcc ggattttagg cggctgatac agaccagcag gaagcgctgt tcctgcaaag
     1981 tgagcttgta gcttgcctcg ataagggcgt ttgatttgta agctatcaga tctgacattt
     2041 cgcttgctcg ttgctattgg tggcgttttt ttactgtacg ccacttatca tatgggtgga
     2101 agtttttttt ttgcaacccc ctttttctgt ggattacacc cccaaaagct ccacctttca
     2161 cccccaaaag ctccaccttt cacccccaaa agctccacct ttcaccccca aaagctccac
     2221 ttatcttgcc agaacatctt taaaatcatg gtgttacaag acctaaaaag gttttaaaaa
     2281 aaagatctaa aaataagatc ttaaaaactt aacggcgagc ctgtggataa cttggggtgc
     2341 tgaaaacaaa aaaccaataa aaacaatgcg ttgcaataaa gcagaattgc aagtttcgaa
     2401 tacaccccct ccaaccctgt ggaaacccag tattcatgcg ggttcagcgc atatcacctg
     2461 ccgaaaaacc gtgcgtatgt gctcaacctg gcgactgatt cacttccgct tcctcgccca
     2521 ctcgctcgct aacgctcggt cgctggggct gcggcgagcg gacgatacaa tcactgttct
     2581 ggcagcgcct ccagctcgtt aatcagcggc agtatgcgcg tcacatcagc ctgtcgtgtc
     2641 acctctgcct tcaggaactg gagtaaccag gcacgcaact gcacctcctc cggctttctg
     2701 acctttgcca gcaatgcagc acccacaaca accttctgcg ctgtgtccag gctccggctc
     2761 ttcgccttca ggcgctgtaa tctcgcctca gcatcagcaa tcttctgttc gagcgttctg
     2821 ctcatgatgt aactccgtgc gcgatgaaaa atcgcatttt agcgcgtcac tggtagttta
     2881 aaaactgagc tggcataatg cacggcaaat cacgaagtgc gcacttatac aatctccact
     2941 ccgtttcgat tgtgtgcgcc ctgcggggct gaaagaaaac tacaaaaagg cattacggca
     3001 gaaatggcga tttatcatct cagcatgaaa attatctcca gaagcagcgg ctacagcgcc
     3061 gtagcttccg ctgcctatcg ttccggttca ctgatgcttg atgagcgtac cggactgact
     3121 cacgactaca cccgcaaaag cggtgtggct gaggctgtga ttctgacccc cgccaccgcc
     3181 cctgcctggt gtacgaaccg tgccgaactc tggaatgccg ttgagaaggc cgaacgccgg
     3241 aagaactcac aacttgcgcg ggaaattgaa ctcgccatcc cccacgaact gccgcaggat
     3301 gccgcacggg aaaccgttct cgcctttgtc agagaaaact ttgtcagtca gggcatgatt
     3361 gctgatgtgg cgttccatca catggataaa acaaatcccc atgcgcacat catgctgacc
     3421 acgagggcta tcggacctgc tgggttcagc ggaaaggtca gggactggaa cgaccggaca
     3481 catgccgaga cgtggcgggc atcatgggct gaccatgcaa acagggcact ggcgaacgcc
     3541 ggttatcagg aagagataga ccatcgttca tacgagcgac aggggcttga gaaaacgcct
     3601 ggtattcacc tcggaaagtc tgcctgtgcg atggaaacac gcggtatcga aacagaacgc
     3661 ggcgaacaga atcgtctgat taacaggctt aacctggaaa tacagatttc ccgcaccgaa
     3721 ctcaggaacg gagggctgac agagcctgcc cgccgtgtgg ctgaccttct gaatatcggg
     3781 cttcctgaca acgcaacttc gtacacacta cgtgacatta tcgaagcgtt gccgaaggac
     3841 agtaactcgg catggaaact caccagtaat gccatgtcga tgatggcaga tatgcaggcg
     3901 acgcgcagac gctgggaaga gctgaacacc gaacgaaagg aagcgatgag ccatgctgcc
     3961 agcctgaaaa aatcatcgcc gttttcgtcc ggtttttccc gtattcccct gatgcaatgg
     4021 gcggcaccgg aataccgtag tgagcaggga aaaatccagt
//
