LOCUS       NZ_CP101239             5284 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-137 plasmid pEC21Z137-5K, complete
            sequence.
ACCESSION   NZ_CP101239
VERSION     NZ_CP101239.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593412
            Assembly: GCF_024298785.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:39:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,062
            CDSs (total)                      :: 4,939
            Genes (coding)                    :: 4,694
            CDSs (with protein)               :: 4,694
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 60 of 245
            Pseudo Genes (incomplete)         :: 170 of 245
            Pseudo Genes (internal stop)      :: 53 of 245
            Pseudo Genes (multiple problems)  :: 36 of 245
            Pseudo Genes (short protein)      :: 3 of 245
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101239.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-137"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z137-5K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-05-31"
     gene            complement(join(4779..5284,1..192))
                     /locus_tag="NML24_RS25170"
                     /old_locus_tag="NML24_25130"
     CDS             complement(join(4779..5227,5227..5284,1..192))
                     /locus_tag="NML24_RS25170"
                     /old_locus_tag="NML24_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_235147823.1"
                     /translation="MASISIRCPSCSTTEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(838..1053)
                     /locus_tag="NML24_RS25175"
                     /old_locus_tag="NML24_25135"
     CDS             complement(838..1053)
                     /locus_tag="NML24_RS25175"
                     /old_locus_tag="NML24_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072754451.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENV
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1060..1551)
                     /locus_tag="NML24_RS25180"
                     /old_locus_tag="NML24_25140"
     CDS             complement(1060..1551)
                     /locus_tag="NML24_RS25180"
                     /old_locus_tag="NML24_25140"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_072739202.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGRQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<1586..2020)
                     /locus_tag="NML24_RS25185"
                     /old_locus_tag="NML24_25145"
                     /pseudo=""
     CDS             complement(<1586..2020)
                     /locus_tag="NML24_RS25185"
                     /old_locus_tag="NML24_25145"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2229..2552)
                     /locus_tag="NML24_RS25190"
                     /old_locus_tag="NML24_25150"
     CDS             complement(2229..2552)
                     /locus_tag="NML24_RS25190"
                     /old_locus_tag="NML24_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007899679.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTAKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGVGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3202..3348
                     /locus_tag="NML24_RS25195"
                     /old_locus_tag="NML24_25155"
     CDS             3202..3348
                     /locus_tag="NML24_RS25195"
                     /old_locus_tag="NML24_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063922569.1"
                     /translation="MHEPPVQSDRCASSGNFQLDSNPERHDKAPLAAATGKRVYKRLLI
                     QSS"
                     /name="hypothetical protein"
     gene            3774..4436
                     /locus_tag="NML24_RS25200"
                     /old_locus_tag="NML24_25160"
     CDS             3774..4436
                     /locus_tag="NML24_RS25200"
                     /old_locus_tag="NML24_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="WP_072661022.1"
                     /translation="MEKNSSDIDVIELFLFLKNKIMSIILFVIFSLILSSIFLLINKNK
                     ISVKYELNMITNSPGMIINCGDDFYCKANMIQSIIKNKSNSITSNIDERGKKINLSWAG
                     DDRYKPSVTAEVNAIHKAINDWYIQDYKTYKSIVNNQDSNYINGTETYAKIALLTKTNT
                     SGERNFISINNEIVNKKYKPALIMTLTLIMAIIFSSSYHIIKRSVLDYKNKSNRSFY"
                     /name="GNVR domain-containing protein"
     gene            4588..>4764
                     /locus_tag="NML24_RS25205"
                     /old_locus_tag="NML24_25165"
                     /pseudo=""
     CDS             4588..>4764
                     /locus_tag="NML24_RS25205"
                     /old_locus_tag="NML24_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     Typing          complement(2668..2840)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="1.98e-82"
     Typing          2765..3371
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.447"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(747..2239)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2236..2552)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="3.04e-59"
ORIGIN
        1 gacgccattc atggccatat caatgatttt ctggtgcgta ccgggctgag aggcggtgta
       61 agtgaactgt agttgccatg ttttacggca atgagagcag agatagcgct gatgtccggc
      121 agtgcttttg ccgttacgca ccacgccttc agtagtggag caggaaggac atctgatgga
      181 aatggaagcc acgcaagcac cttaaaatca ccatcataca ctaaatcagt aagttggcag
      241 cattacctgt atttcagctt catcaatccc gtttttttta ctgaatccat ttaccaatat
      301 cctgtccata cgttactaaa gaaaataata tatacggaat gagttatcat gcgcctaatt
      361 taccataaca tccgttgcgt gcaccgctga aacggggttg gctgcgtttc cggtgccaga
      421 cgcgagaaag tggcgacaga cataacgaaa aaacaccaga aacgcagccg ctgaatatta
      481 cactggtgca tagtcatgca gccagatgca tggatgttaa agtcacacta aaatccgcgc
      541 caatgcctta aaaaccgaca aatgcttctc cagccagtcc cacagctccg ccgccagata
      601 caggcagtta agcaatgatt caataagatt taacagcttc atttcccgct cctgaataag
      661 tcgtgacctt tttgatcacg aacctattta gcgggagaaa taagagcccc tcttcgtgcg
      721 ggttgtcagc tgcctggatg gtttaaagtg tcggcccgcg gtaggttttc tgcggctggc
      781 gctccagctc cagtgttttc tgatactcat tctccctctg ccggagctcg atggcattca
      841 gttcccggac aagcggcgca gctgctcgct taaactcagc acctgcgtgc tcaagtccga
      901 cacgcgctcg ttcagcacca cgttctcccg ttgcataagc ccacacatgc tccgccattc
      961 cgcgaaggcg ctctcccatt cgtccagcct ttgcgagtag tcctgctgta gctgctctaa
     1021 tgcgctcagc aactgctttt ccagctctgt cattttccgc tactcctgtt tcaggcggat
     1081 ccatgtcgtc ccgtcgtagg ggatggcttc ggtttgcatc cccggcggca ggataagaaa
     1141 cctccggccg tcgctgctct cctgataacg cactccccac gtccgggcgg tcattttcgc
     1201 cagggtatct tcctgctgac gaagatgtgt gtaattgtcg gttatctgcc gtccctgcca
     1261 ccacagaatg ctcccgctcg tggcgatcag cagcgctgac accatcagga tggtgagcca
     1321 tgtcctcccg gccgtcagca acacgctccg ccggtttttc tccatcgcct cgctcatact
     1381 ctgctcgtgg gcgctgatgg cgtcgctgat tctcctcgcg cttgcgttca gttccgctct
     1441 gacagactgc tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac tcttctgctg
     1501 cgctttcgat tgctgctcta agtccttcgc cagcgttaaa agactgctca tagatggctc
     1561 ccttcagtcg gatgtttcgc cccccgtcag ggtcggcaat gctgatactg cttttcgttg
     1621 tacgcaccac ctcaaaacct gcaccttcaa gagcctcagt gacgtcctga cggcttttca
     1681 gctccccgga cgaggctaag ctcagcagtc cacgggtgat ggcttctgcg gcttcctgct
     1741 tcgttttcgg cagtccggcc ggcgtgacca gcgcccggcg gttttccggc gcgttcggat
     1801 cgtgcagccc cagtctgccg ttcacgatgg tctgccaggc atcgatacgc ggccggtcgg
     1861 cgcggtcgta gtacggctgg agccgcctgc cggtcagcag ctcggtattg gggatcagaa
     1921 agttcagctc cagacgcccc ttgtcctcgt gctccaccca cagaatgctg tactgatctt
     1981 tatcaagtcc gggcatcaga acccgctcga agctggccat cagtttttcc cgctggccgg
     2041 gaggcagatc ggcctccgca aacgacagga cgccggaggt gtatttcttc acgtaaggcg
     2101 acgcgtcgat cagctcccgg acttcttccg gcttcccctg caggacgctg gcgccttcac
     2161 gctgccggtc tttacccagc agatagtcca ccggcccggc gccaccgccg cgaccgcgtg
     2221 gatgaaattt aacgatcatc gtccgttcct ttctcaagaa cggcgtgccg cagccgctca
     2281 agcccggcat cgatggccat cagcgccgcc acaatctgca cccggtcatg accggtaccg
     2341 acgccggcgt taacacggcg ggctatctgg ttcaggttgt tgcccatacc ggcaagctgg
     2401 cgcagcagcg ccggcgagat cgaaggaagt tttgcggtac gagccggctt ttcgtccagg
     2461 caggtctgcc gcatccacgc cgccagctgc ctgccgtcgc agcgctcaag tagccgcctg
     2521 tgctcatcct ccgtcaccca catggtgagc atcttgttcc gtttctctga caaaacagtc
     2581 tcctgataac cccgggacgg gcgggaaaac ctttcccggt cggcgcgggg ttggacaagc
     2641 cgcaggcgcg tcagtggctg ttagcgggtt tcggggcgca gccctgaacc agtcacgtag
     2701 cgctagcgga gtgtatactg gcttactatg ttggcacgga tgcgaatatc agtgaagtgc
     2761 ttcatatggc aggagaaaaa tgcagcagcg gagcgtcagc agaatatgca atacaggata
     2821 tattccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     2881 ccggcttaca ggcggggcgg aaatttcctg ggagatgcca gaaagaagct taacagggaa
     2941 gcgataaggc cgcggcgaag ccgtttttcc ataggctccg cccccctgac aagcatcacg
     3001 aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt
     3061 ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     3121 ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggaaggca
     3181 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctcc
     3241 tccggtaact ttcaacttga ttccaacccg gaaaggcacg acaaagcgcc actggcagca
     3301 gccactggta agagggtgta caagagatta ctgatacaga gttcttgaag tcagagccaa
     3361 actacggcta aactggaagg acgaatttgg tggcagcggt cttgtacacc ctgttaccac
     3421 ggttcagaag ttccccaact tactgaacct tcgaaaaacc acctccccag gtggtttttt
     3481 cgttttcaga gcaagagatt acggctgtaa cgcaaggatc tcaagaagat cttaaaatat
     3541 gttgaaagga tacaggctaa caacaactcc ctccatagac atgaccgaac aaaaaagaaa
     3601 caaaaaaaat gattttagga gttttttcac acctaaatag taggtaaaat gttcttttat
     3661 tcgattggct tttaaaatga aatacactaa atccgcaaaa atattcatca cagccttttt
     3721 aattttattt atttatttaa cccatacttt aataaatatt attggtatat aaaatggaaa
     3781 aaaattcatc tgatattgat gttatcgagt tatttctctt tctcaaaaac aaaataatga
     3841 gtattatttt gtttgttatt ttctcattga ttttgtcatc tatttttttg ttaataaaca
     3901 aaaacaaaat aagtgtaaag tacgaattaa atatgatcac taattcgcca ggtatgatta
     3961 ttaactgtgg ggatgacttt tattgcaaag cgaacatgat acaatcaata ataaaaaaca
     4021 aaagcaattc tataacatca aatatagatg aaagaggaaa aaaaataaac ctgtcatggg
     4081 ctggtgatga ccgttacaaa ccctcggtta ccgctgaagt aaacgctatc cataaagcta
     4141 ttaatgactg gtacatccaa gattataaaa cttataaaag catagttaac aaccaggata
     4201 gcaactatat aaatggcact gaaacatatg caaaaattgc attattaact aaaacaaaca
     4261 cttccggaga aagaaacttt atttcaatca ataatgaaat agtaaataaa aagtataaac
     4321 cagctttaat tatgactcta acgttaatta tggctattat cttttcctcc tcgtaccata
     4381 taataaaaag atctgtactt gactataaaa ataagtcaaa taggtctttt tattagcctg
     4441 taacgattaa taaccgtatt ctagcaacat ccgttacacg cctgatgcag aaaagcccgc
     4501 taatagcggg cctcccgaca aatctcactg ggtgatgcct ctaattagtt gaatctgatg
     4561 tataatacgg gcttttgagg ttatctcata gccagcgtta atattcattg tccccgttgt
     4621 tagtcagctc gggtttaccg ccatggtcag aaccctaaag gccgtgacag atttcgctgc
     4681 cgtgactgcc accgtgtatt tcagctcact tatacttatc aagcacgtaa gccgggtatg
     4741 aaagagctga tcactgaaat ggcggtaatg actccaactt actgatagtg ttttatgttc
     4801 agataatgcc cgatgacctt gtcatgcagc tccaccgatt ttgagaacga cagtgacttc
     4861 cgtcccagcc ttgccagatg ttgtctcaga ttcagattat gtcgctcaat gcgctgagtg
     4921 taacgcttgc tgataacgtg cagctttccc ttcaggcgtg attcatacag cggccagcca
     4981 tccgtcatcc ataccacgac ctcaaaggcc gacagcaggc tcagaagacg ctccagtgtg
     5041 gccagagtgc gttcaccgaa gacgtgcgcc acaaccgtcc tccgtatcct gtcatacgcg
     5101 taaaacagcc agcgctgacg tgatttagca ccgacgtagc cccactgttc gtccatttca
     5161 gcgcagacaa tcacatcact gcccggttgt atgcgcgagg ttaccgactg cggcctgagt
     5221 tttttaagtg acgtaaaacc gtgttgaggc caacgcccat aatgcgtgca ctggcgcgac
     5281 atcc
//
