LOCUS       NZ_CP101231             2089 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-144 plasmid pEC21Z144-2K-1, complete
            sequence.
ACCESSION   NZ_CP101231
VERSION     NZ_CP101231.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593413
            Assembly: GCF_024298485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:27:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,464
            CDSs (total)                      :: 5,340
            Genes (coding)                    :: 5,114
            CDSs (with protein)               :: 5,114
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 79 of 226
            Pseudo Genes (incomplete)         :: 163 of 226
            Pseudo Genes (internal stop)      :: 45 of 226
            Pseudo Genes (multiple problems)  :: 53 of 226
            Pseudo Genes (short protein)      :: 3 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101231.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-144"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z144-2K-1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            224..1189
                     /locus_tag="NML25_RS27270"
                     /old_locus_tag="NML25_27265"
     CDS             224..1189
                     /locus_tag="NML25_RS27270"
                     /old_locus_tag="NML25_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1193..1459
                     /locus_tag="NML25_RS27275"
                     /old_locus_tag="NML25_27270"
     CDS             1193..1459
                     /locus_tag="NML25_RS27275"
                     /old_locus_tag="NML25_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          618..849
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gcccagtaat gacgaggctt tgagtgggtt ttgacaggtc aaagaaaatg gagcagaatt
       61 gaggcgtttt taatcggcgt tggggagtgc gtcaacactc cccaacattt cgaatgtgtc
      121 acctcagcgg caaactctgg tgacatgtac tggctcgcaa tgcacaggta cgtgatgaat
      181 ataccacatc aaatcacagc ctgcccagat cggagcaggc ttaatgtcag aagataaatt
      241 cctttcggac tacagccccc gtgatgcagt ttgggatacc cagcgcacgc ttaccgattc
      301 tgtcgggggt atctaccaga ctgctgctga attcgagcgc tatgcactcc gtatggcctc
      361 ctgtagcggt ttgttacgtt ttggttggtc taccatcatg gaaaccggag aaacgcgcct
      421 acggcttcgt agtgcgcaat tttgccgtgt ccgtcattgc cctgtctgcc agtggagaag
      481 aaccctcatg tggcaagccc gtttttatca ggctctaccg aaaatcgttg tggattaccc
      541 gtcttcccga tggttgtttc tgacgttaac tgtcaggaac tgcgagatag gtgaacttgg
      601 aacagtcctt acagcaatga atgcggcgtt taagcgaatg gaaaagcgaa aggagctatc
      661 acctgttcag gggtggatca gggctacgga ggtgacgcga ggtaaggatg gcagcgcaca
      721 tccgcatttt cactgtctgc tgatggtgca accttcttgg tttaaaggga agaactacgt
      781 taagcacgaa cgttgggtag aactctggcg cgattgcttg cgggtgaact atgagccgaa
      841 tatcgatatt cgggcagtaa aaactaagac aggtgaggtt gtggccaacg ttgccgagca
      901 actgcaaagc gcggttgctg aaacgctgaa atactccgtt aaaccggaag atatggcaaa
      961 cgatcctgag tggtttcttg agctgacgcg gcagcttcac aagcgccgtt ttatctcgac
     1021 cggtggggcg ctaaaaaacg tcctccagtt ggatcgagaa accaatgagg atcttgtcat
     1081 tgccgacgat gtaggggatg gcactgatga cgggaagcgg acggcgtttg tctgggattc
     1141 aggtaaacgg cgttacaaac gcgcccctga gaaggataaa tcggattaac gtatgaatat
     1201 taatattgaa tacctgaatg gaaataagac tattggttta ttttttttaa gaagtgaagc
     1261 ggtgattcct gacaggttta aaaaccttat tttgcttatt gatggattaa gttttggcac
     1321 atttggtttt catccgcacg aaggttttga ggatgaatta attttatata ttcagaaaac
     1381 aaacgagagg gtaaaaactc tttttgtgaa aatagattta aacggtatca agtttggatt
     1441 tttaagaacg cattcttagt tctggaaaag agccagcggc aggctgaggt gataggtacg
     1501 agattgcatg caatctctag tgctctgtct atcctgcatt atcctcagca ttatcctcag
     1561 ccttgccaac tcgacaccaa tgcaggatag acaatccgat gtcaaatgtt aacactctgc
     1621 gagtggtaca ttttccccgg attatcgtcc tgagcctgcc gctggctctc tttctaccgc
     1681 ctcgctttgc tcgttgctca acgcctcaca gacacggatt aaaatccgca tccgttcacc
     1741 gttttttaaa gtccgttaaa agcatgatgc catctccgag agttaatctc gtcaaatgct
     1801 aaatcgtggg ggtccccttt ggggttccga tttagtgatt gacgacacca ccgattaaaa
     1861 aacttatgcg gggtggatgg tttcacgaag tgaggccatc cacctgtaag acagggtttt
     1921 gtttttattc cctgttttag tgatcgggtg tgtggaaaag gttggggtaa gccgttcggg
     1981 ggtgcttgtt ttggggggtt aaaattgtgg ttattttttg cgcaattctc gcgcgtgatc
     2041 cttgtattta tacttaaggg ataaatggcg gatatgaaat agtggttta
//
