LOCUS       NZ_CP101230             4072 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-144 plasmid pEC21Z144-4K, complete
            sequence.
ACCESSION   NZ_CP101230
VERSION     NZ_CP101230.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593413
            Assembly: GCF_024298485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:27:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,464
            CDSs (total)                      :: 5,340
            Genes (coding)                    :: 5,114
            CDSs (with protein)               :: 5,114
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 79 of 226
            Pseudo Genes (incomplete)         :: 163 of 226
            Pseudo Genes (internal stop)      :: 45 of 226
            Pseudo Genes (multiple problems)  :: 53 of 226
            Pseudo Genes (short protein)      :: 3 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101230.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-144"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z144-4K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            join(3841..4072,1..461)
                     /locus_tag="NML25_RS27255"
                     /old_locus_tag="NML25_27250"
     CDS             join(3841..4072,1..461)
                     /locus_tag="NML25_RS27255"
                     /old_locus_tag="NML25_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(717..2876)
                     /gene="mobQ"
                     /locus_tag="NML25_RS27260"
                     /old_locus_tag="NML25_27255"
     CDS             complement(717..2876)
                     /gene="mobQ"
                     /locus_tag="NML25_RS27260"
                     /old_locus_tag="NML25_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3055..3309
                     /gene="mobC"
                     /locus_tag="NML25_RS27265"
                     /old_locus_tag="NML25_27260"
     CDS             3055..3309
                     /gene="mobC"
                     /locus_tag="NML25_RS27265"
                     /old_locus_tag="NML25_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2519..2877)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          2878..3054
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          118..461
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.74e-180"
     Typing          3842..4072
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.414"
                     /coverage="100.0"
                     /evalue="2.4300000000000002e-107"
     Typing          complement(721..2963)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaggtcgat cattctcaag aatgacgaga agcgtgagga gttccgctgg atccagtatc
       61 gggcacagta cgctaaaggt gaggccaagg ctcgaataac cttttctgac gcggtaatgc
      121 cttatctgac gcaactgaag gggcaattca cgcgcgttgt tataaaaaac atatcaggtt
      181 tatcaagtag ttacagtata agaatatacg agttcctcca gcaattccgc agtacgggag
      241 agagaattat tgctcttaat gactttcgtt caatgttagg gattgaaaat aaatacaaac
      301 aattcagaga cttaaataaa atactaataa aaccctgtgt tgacgaactg aacaaaaaaa
      361 gcgacctggc tgtaacggtt gagacgatca aaaaggggcg cacggttgta gccctgcatt
      421 ttcgcttcaa ggaagacaaa cagatcaaga tgacgatcta aaatcgattt taaggcacct
      481 aaaacgctcc ctgctgcgtt ttttttgtgt tagtagtgcg aaggtactat tgaagtgaaa
      541 aattgcgtca aaagcgctct aatgcagcga aaaaagaacc cccaattttt gggggcggaa
      601 aaacaaaact atgcttgcat tatgtgtgtg cttcttgcat tcatccctgt cgatgaacat
      661 agaagaacat cgacgaacat aacacgatgt taaacaaaac aaaatattgt tcacattcac
      721 tgtgaccaac cgcgatcatg tctcctctcg gtggtgtgtg ttcttgcatc ttcctcgcgc
      781 cgccattcat cccaggtacg attgaaaccc agagagcgga acagatgatc cggctgtttt
      841 tctggtgtgt tgacggcagc aaaaagcggt gcctcacggg ttttcagatg aaactccata
      901 agttccggcg caggtgatat gtggctgtct gcatcgtgat ttacggcaac accataccct
      961 gcataccgta accccttatc ggtcagtgca ctgatgacca gctggctgtt atcggctgag
     1021 ggaacggcaa acgcgggata cagcgtacca ttaagccgga caacaccgcc cagagagtca
     1081 tccacgccca tgaccagcaa aagctgaagc tggcgggttt cccggtcgac agaactcatt
     1141 accggcatca gcgtcggtag cagacgtccc tgcgtaaaat ccaccttcct tgctgccagt
     1201 gatggcatgg ctggctggcg gtcttctcca agacagaaag actccggcca tgtcttcggc
     1261 tggcggagat tgcgggtata ttgcggccat gtctggcaaa cctgcgtcag aagttgctca
     1321 aaggtcgcag aggaataaac acgttgcagt ggtggatgtt gaccgatttc gcttcggtca
     1381 cagatataac cactcctgtc acgcattaaa tcaacacgct caaagattct ggtgcggtat
     1441 ttctccagct cgttccccca gagcgtgtat tctccgttcc ggttgtgaag cagaaggtaa
     1501 caggtcattt ccccgttacc aggcatgggg gcctcgccat accccgtaac gactgcgctc
     1561 agtacccctg aatccataac ggcattgtca tttttcctga gctgttccgc tcgtcgctcc
     1621 gcgcgactac gttctgtatc ctcctgctcc ttccggcgca attcctcttc acgtttaagc
     1681 aactggactc tgagagctgc aaggccggat gccccccact gctgccactg tttttcaaat
     1741 gccgtctgac tcgcgggaat gtcctgtttc tccaccttcc ggtaatgctg tcgcaatttt
     1801 tccatttgct ggctcagcga ctggattttt ccctgttcgc tacggtattc cggtgccgcc
     1861 cattgcatca ggggaatacg ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg
     1921 gcagcatggc tcatcgcttc ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc
     1981 gcctgcatat ctgccatcat cgacatggca ttactggtga gtttccatgc cgcattactg
     2041 tccttcggca acgcttcgat gatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc
     2101 acgatattca gaaggtcagc cacacggcgg gcaggctctg tcagcccgct gttcctgagt
     2161 tcagtgcggg aaatctgtat ttccaggtta agcctgttaa tcagacgatt ctgttcgccg
     2221 cgttctgttt cgataccgcg tgtttccatc gcacaggcag actttccgag gtgaatacca
     2281 ggcgttttct caagcccctg tcgctcgtat gaacgatggt ctatctcttc ctgataaccg
     2341 gcgttcgcca gtgctctgtt tgcatggtca gcccatgatg cacgccacgt ctcggcatgt
     2401 gtccggtcgt tccagtccct gacctttccg ccgaacccag caggtccgac agccctcgtg
     2461 gtcagcatga tgtgcgcatg gggatttgtt ttatccatgt gatggaacgc cacatcagca
     2521 atcatgccct gactgacaaa gttttctctg acaaaggcga gaacggtttc ccgtgcggca
     2581 tcctgcggca gttcacgggg gatagcgagt tcaatttccc gcgcaagttg tgagttcttc
     2641 cggcattcgg ccttctcaac ggcattccag agttcggcac ggttcgtaca ccaggcaggg
     2701 gctgtcgcgg gggtcagaat cacagcttca gccacaccgc ttttgcgggt gtagtcgtga
     2761 gtcagtccgg tacgctcatc aagcatcagt gaaccggaac gataggcagc ggaagctacg
     2821 gcgctgtagc cgctgcttct ggagataatt ttcatgctga gatgataaat cgccatttct
     2881 gccgtaatgc ctttttgtag ttttctttca gccccgcagg gcgcacacaa tcgaaacgga
     2941 gtggagattg tataagtgcg cactttgtga tttgtcgcgc attatgccag ctcagttttt
     3001 aaactaccag tgacgcgcta aaatgcgatt tttcatcgcg cacggagtta catcatgagc
     3061 agaacgctcg aacagaagat tgctgatgct gaggcgagat tacagcgcct gaaggcgaag
     3121 agccggagcc tggacacagc gcagaaggtt gttgtgggtg cagcattgct ggcaaaggtc
     3181 agaaagccgg aggaggtgca gttgcgtgcc tggttactcc agttcctgaa ggcagaggtg
     3241 acacgacagg ctgatgtgac gcgcatactg ccgctgatta acgagctgga ggcgctgcca
     3301 ggacagtgat tgtatcgtcc gctcgccgca gccccagcga ccgagcgtta gcgagcgagt
     3361 gggcgaggaa gcggaagtga atccgtcgcc aggttgagca catacgcacg gtttttcggt
     3421 aggtgatatg cgctgaaccc gcatgaatac tggatttcca cagggttgga aggggagtat
     3481 tcggaacttg caattctgct ttcttgcaac acattgtttt tattggtttt ttgttttcag
     3541 cgccccaagt tatccacagg ctcgccgtta agtttttaga tcttgttttt aagatcttgt
     3601 ttttaatact tgttttaggg tcaaaattca ttaataaaat caatatgtta tagaggtata
     3661 tgtggaggaa tttaaggtaa aggtggagga atttaaggtg tttaggtgga ggaatttaag
     3721 gtaaaggtgg aggaatttaa ggtgttattc acagaaatgt gaggatataa aaaaaaatat
     3781 tccacatttc ttatttgtgg ggcacattaa agcatcacca catttgaacg ggatctttgt
     3841 atgtcggact tgattgccta caagtcgaat gctttggttg aggccagcta caagttgacc
     3901 ctacaggaac aacgttttct gttgctgtgt atcagtcgat tgaaatctgg tgctgatgct
     3961 gaattgcaaa agacaatgac cattaccgct gcggaatatt ttgattcatt ccctgacatg
     4021 gggcggaaaa atgccgaggt tcagttacag gaagcgatcg atcgtttatg gg
//
