LOCUS       NZ_CP101228            24263 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-144 plasmid pEC21Z144-24K, complete
            sequence.
ACCESSION   NZ_CP101228
VERSION     NZ_CP101228.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593413
            Assembly: GCF_024298485.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24263)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24263)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:27:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,464
            CDSs (total)                      :: 5,340
            Genes (coding)                    :: 5,114
            CDSs (with protein)               :: 5,114
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 226
            CDSs (without protein)            :: 226
            Pseudo Genes (ambiguous residues) :: 0 of 226
            Pseudo Genes (frameshifted)       :: 79 of 226
            Pseudo Genes (incomplete)         :: 163 of 226
            Pseudo Genes (internal stop)      :: 45 of 226
            Pseudo Genes (multiple problems)  :: 53 of 226
            Pseudo Genes (short protein)      :: 3 of 226
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101228.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-144"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z144-24K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            complement(301..606)
                     /locus_tag="NML25_RS27050"
                     /old_locus_tag="NML25_27045"
     CDS             complement(301..606)
                     /locus_tag="NML25_RS27050"
                     /old_locus_tag="NML25_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dpoa decarboxylase"
                     /protein_id="WP_094283554.1"
                     /translation="MLKEDMFINNIHSRNQDRIHVKRVFDDISNKANRGCGLYYEIYQA
                     RLMRGLIEHTVKLKSSDANKLISYAASQGYEITEDRYNAVIKAEDECLTEITRAQV"
                     /name="dpoa decarboxylase"
     gene            complement(634..849)
                     /locus_tag="NML25_RS27055"
                     /old_locus_tag="NML25_27050"
     CDS             complement(634..849)
                     /locus_tag="NML25_RS27055"
                     /old_locus_tag="NML25_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283555.1"
                     /translation="MNTDENMVFYLTNSDLVYSYAHKIVHSIPSGMKGKFITSIGETIE
                     DLKKRHPDIILITESEALALQSERKN"
                     /name="hypothetical protein"
     gene            complement(852..1151)
                     /locus_tag="NML25_RS27060"
                     /old_locus_tag="NML25_27055"
     CDS             complement(852..1151)
                     /locus_tag="NML25_RS27060"
                     /old_locus_tag="NML25_27055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_000716758.1"
                     /translation="MKKIAAITMFVLTTATPVYSYAADPCEVVLCMYGKATGNSGGGEC
                     RSAEKAFFNILKKKKGSIRWSKTFDARKAFLNQCSSADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(1181..1579)
                     /locus_tag="NML25_RS27065"
                     /old_locus_tag="NML25_27060"
     CDS             complement(1181..1579)
                     /locus_tag="NML25_RS27065"
                     /old_locus_tag="NML25_27060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_094283556.1"
                     /translation="MIKHILVTVFVTLITGCSSPPPPVPVEWNKKPVSLNDKLPEWKPN
                     SLIIKSPVINDKWTLSISSQDFNKEDWMPSVFYAIAHSTQITVATKSGADYFSTKNWLY
                     HHGANGVIIYHPIINCLTCNETTIYFSR"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(1576..2601)
                     /gene="virB11"
                     /locus_tag="NML25_RS27070"
                     /old_locus_tag="NML25_27065"
     CDS             complement(1576..2601)
                     /gene="virB11"
                     /locus_tag="NML25_RS27070"
                     /old_locus_tag="NML25_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125556.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_044862971.1"
                     /translation="MTVKNISLDRYKQRFFSEYLELPGLTEIAVNRPGELWTKIDGVWG
                     QHTVDLKFDDCYNFARCLAKHHNDNIEDIKPGLSATLESGERCQVVVPPACERDTISIT
                     IRKPSKIQIPHQAYIDSGFYNRVTGIEKASTHNEELIELYKSKNIPLFMEKCVEYGKTL
                     AVAGETSTGKTTYMKMLIQYIPVHLRITTIEDNPEIIFYKHKNYVHLFYPSESSDEKGS
                     VVTPAALIRWNYRMNPDRILLTEVRGGEAWDFLKITGSGHEGSMTSIHAGSAHEAIDGF
                     ITRCTENPQCAQLPYSFLLRKVLDSLDVVVSIDLDGNIRRMNDIYFRPLHRNQYYEEMK
                     V"
                     /name="virB11"
     gene            complement(2605..3855)
                     /locus_tag="NML25_RS27075"
                     /old_locus_tag="NML25_27070"
     CDS             complement(2605..3855)
                     /locus_tag="NML25_RS27075"
                     /old_locus_tag="NML25_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="WP_146999495.1"
                     /translation="MTDENKSPSTEEEKPVTTPDQDDIATLEREARAKREAELQALQDD
                     EKENSLQPAVNKLKKRPKGKATAFLAICAVALIGLTWGGNWVYQNFLTQEDGEKTKEVT
                     PPKKTNDYRKRTDLGMATNEPENSTEGEQTVNQDNTGNVAGTAGQMKTFEPPQLNKASF
                     LVRRNNTASPQNQVKTRQQEMILTAENNATQPTDSTATTTQHGTASQAISPATVRRIPY
                     NPDLYIPENTVIPCSMDYRFVSDRAGRIRCTVAKDIWSASGNTKLIEKGTTATGIYQTG
                     ITQGQGRAFIMMTKLRTRQRPYLDIPLIDTNAAGELGESGVDGWIDTHFWERFGGALMV
                     GMIPDIGAWASNNAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQGEIINL
                     ITGQDIDFSNIYTLRMK"
                     /name="TrbI/VirB10 family protein"
     gene            complement(3897..4802)
                     /gene="virB9"
                     /locus_tag="NML25_RS27080"
                     /old_locus_tag="NML25_27075"
     CDS             complement(3897..4802)
                     /gene="virB9"
                     /locus_tag="NML25_RS27080"
                     /old_locus_tag="NML25_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_094283540.1"
                     /translation="MRKLTLITLIIMLNSQAWAAATPQASRYDARIQQVIYNPQNVTVV
                     NTKPGFMTTLVFDNDEAVISASPGFDEAWEARADANRVNIRPVALTQGAPGEDGNTTQV
                     VIPPNSRDWHTNMLIVTNKRLYNVELNVIDEKSPQQPAYLVSYRYPGDERNRANKEAAA
                     RQKEWEQKQEQAQIQKALRTAQTPRNWDYTKYPGKDSYSIEPDFAYDDGRFTFIGFSPN
                     KSVPSVTKEQNGQEYVVNSSIQRKGNFTVLVIQEITPRLVLRSGDAVVGLKNNGFGKVH
                     ATDNSTVSDKVERVEKTDNI"
                     /name="virB9"
     gene            complement(4802..5485)
                     /locus_tag="NML25_RS27085"
                     /old_locus_tag="NML25_27080"
     CDS             complement(4802..5485)
                     /locus_tag="NML25_RS27085"
                     /old_locus_tag="NML25_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000139289.1"
                     /translation="MTEQKLIADSRSFEQQMIDRDKRATKAGFLVGGAGLLIAILSLIA
                     IVIMLPLKQTTVELYTVDNHTGRVEHVTRASKTALTAEEAYQKAMAANYVKLRERYVYP
                     ALQDDYETVQIYNSPQVNDDYLALYAGKNAPDKVYNNAAHTVKIEIISEQITNATAPDK
                     VATVRYKKIIRRMADNSIRNEYWDARFTFHSDPEKEMSDVEREINPFGFTVTSWQTDRE
                     IRGGE"
                     /name="type IV secretion system protein"
     gene            complement(5478..5621)
                     /locus_tag="NML25_RS27090"
                     /old_locus_tag="NML25_27085"
     CDS             complement(5478..5621)
                     /locus_tag="NML25_RS27090"
                     /old_locus_tag="NML25_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000825481.1"
                     /translation="MKRLIALALLCVLSGCAQKSKSLPPAFSGQGSPVNTPQIIAELTT
                     HD"
                     /name="hypothetical protein"
     gene            complement(5669..6739)
                     /locus_tag="NML25_RS27095"
                     /old_locus_tag="NML25_27090"
     CDS             complement(5669..6739)
                     /locus_tag="NML25_RS27095"
                     /old_locus_tag="NML25_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_024187435.1"
                     /translation="MMAGIFSSVEKNIIGGITDATNGLMMSYSSMMMGLAATSATIYIM
                     WRGYQTLAGKLSTPMEDVMWDIMRMAIILSFVANINGYLDGVIDAVNGIKEGFSSSTSI
                     WQLLDTLWNSARVLGKTLHDLDNSEFIKEEGTIAELFVWGGVFVLMIITAFVSMIAEIM
                     ILLLGITAPIFIFCLMYGFLRPMFNNWLQNIFAAILTIMFSALALRIVIGFLTSRMSEA
                     VTQASKANIVELGAQVCLAGVCAAILVYMSAKLAGALAGASATATMQGAAALGIGAAAY
                     GAGKMLKEAGKVLPKEVASPSNKGLGDPQSRIVFNAADARKHAIEQMIARNEARMLTDQ
                     SAPKLPPSIKRLPPPR"
                     /name="type IV secretion system protein"
     gene            complement(6749..6976)
                     /locus_tag="NML25_RS27100"
                     /old_locus_tag="NML25_27095"
     CDS             complement(6749..6976)
                     /locus_tag="NML25_RS27100"
                     /old_locus_tag="NML25_27095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010617327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000720509.1"
                     /translation="MKKIILFTIISGLMALTSGCKEEAKTTKWYREHPDELKVVYAECQ
                     KTGSASENCKHANEAHWQIQQLNAPEISFN"
                     /name="EexN family lipoprotein"
     gene            complement(6988..7692)
                     /locus_tag="NML25_RS27105"
                     /old_locus_tag="NML25_27100"
     CDS             complement(6988..7692)
                     /locus_tag="NML25_RS27105"
                     /old_locus_tag="NML25_27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_032173316.1"
                     /translation="MKRFRPLILAVLIASPIVNAAGIPVFDATARLENIRQWTKEANQW
                     LQTVQHYKDQLQAYQKQLETVTGIRDIQAFVNQAKSLKADLDKLRKPGQALNDLLLSGG
                     SSGQFDELYNKYKIFDNCNAEQSKSYANTCRQEVINKAIQIEQTNEVQTQIGEMLGEIN
                     ELSDRVANAKDMKEAQDLANSIQLKSVMLNTLTTQWEMNVKAAEKREKMLAEERRKKWN
                     QQQLNAPEIMFN"
                     /name="type IV secretion system protein"
     gene            complement(7707..10451)
                     /locus_tag="NML25_RS27110"
                     /old_locus_tag="NML25_27105"
     CDS             complement(7707..10451)
                     /locus_tag="NML25_RS27110"
                     /old_locus_tag="NML25_27105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="WP_001096401.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLTVVGVTIIVAIWTSIALLLFLP
                     IEFLVMKSLTRNEPLRFNLIAVWLRVRGNPVANRLFGATAFMPVEHNAVDIQEFIDAMK
                     LNQCATIKKYIPYSSHIHPNVVRSLNSDFFCTWELTGTPFDCESDETLQFGTNQLHGLI
                     RSFEGFPVTFYVHNSREQFTDSLHKVSGNPYADTVSERYYASLNKRPFRRNRLFVTICY
                     RPFASLDKANRKRMKDGQKLKEIDSALLEVLEIKSTLDTALSRYGAHALGTFTENEAVF
                     SSQLAFYEYLLTHQWRKVRVTRTPAYNVMGNVALYFSAESGQINHVNGTQYFRGLEVKE
                     FSEETATGMTDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLMMSADDDAVSQRLDL
                     DVALDLLTSGKIAYGKHHLSIMVFSPSLESLIADTNEISNALNNIGITPVPAEISLSAA
                     YMAQLPGNYNLRPRAGELSSLNFVELASLHNFYPGKRDKAPWGDAMALVRTPSGDGYYL
                     NLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFLQINQQKYNRDDSFSPDARTKRL
                     TTVYLDKDRGAEINIRALGGKYYRVFSGEPTGWNPFALPATKRNINFIKQLMKILCTRN
                     GATITPRDERRLNDAVNAVMSDEPQYRVYGITRMLENLPEPATREAQENGLSIRLSQWA
                     RGGEFGWVFDNEHDTFDISNCDNFGIDGTEFLDDPNVCAPISFYLLYRITSLLDGRRLV
                     IFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDEIAPAVIEQCGTQ
                     ILAANPSADRSHYVDGMKFEPEVFDVIKGLDPQARQYVVVKNQFRRGDTKRFAARITLD
                     LSGIGRYTKVMSGDAPNLEIFESLYQEGMKPSDWLDSYLAKAL"
                     /name="VirB3 family type IV secretion system protein"
     gene            complement(10464..10760)
                     /locus_tag="NML25_RS27115"
                     /old_locus_tag="NML25_27110"
     CDS             complement(10464..10760)
                     /locus_tag="NML25_RS27115"
                     /old_locus_tag="NML25_27110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_029397047.1"
                     /translation="MFNKLKAICSRASAYILAPLVSVVSPVFAADGFSRANTIMEKVSN
                     GLHGLAAVTITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
                     /name="TrbC/VirB2 family protein"
     gene            complement(10764..11468)
                     /locus_tag="NML25_RS27120"
                     /old_locus_tag="NML25_27115"
     CDS             complement(10764..11468)
                     /locus_tag="NML25_RS27120"
                     /old_locus_tag="NML25_27115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_094283541.1"
                     /translation="MLSTVSFMAVAMQCAATVHPSTSLDVARVESGFNPYAIAEIIPKR
                     ERQPGDKGFISHMPKTKEDALSIVKQIEAKGRRYSVGLMQITSTNFNSYAVTAADLFNP
                     CTNLSVFEKIITDCYQRGGTLKRALSCYYSGNFTTGQQPEAALSRTSYIQRIGYSPEKP
                     RYVVPGTKDDIATQSAILNATPVEAPARPRVVWPGAIVRGVPAQLRQKKADTVYYPAQV
                     IRGNRFSTEKED"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(11484..11690)
                     /locus_tag="NML25_RS27125"
                     /old_locus_tag="NML25_27120"
     CDS             complement(11484..11690)
                     /locus_tag="NML25_RS27125"
                     /old_locus_tag="NML25_27120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_029397048.1"
                     /translation="MPKPTYNVYVTQEVPTDENGGKNTYWTKVGAAFAHNGKPGLNIML
                     VPGIAVSGKLVLLEPKEDTPQGN"
                     /name="hypothetical protein"
     gene            complement(11691..11903)
                     /locus_tag="NML25_RS27130"
                     /old_locus_tag="NML25_27125"
     CDS             complement(11691..11903)
                     /locus_tag="NML25_RS27130"
                     /old_locus_tag="NML25_27125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001133545.1"
                     /translation="MPNPIGQAKSTAVIYIGKKRYETLAKHARDISYVAEVNIRPSAFL
                     QYMIDKLSNEAHNEMLKELMSKEDK"
                     /name="hypothetical protein"
     gene            complement(11980..12429)
                     /locus_tag="NML25_RS27135"
                     /old_locus_tag="NML25_27130"
     CDS             complement(11980..12429)
                     /locus_tag="NML25_RS27135"
                     /old_locus_tag="NML25_27130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998906.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="WP_249540331.1"
                     /translation="MNAWYLLYCNNNELDRIAYRCKTMNVVAFCPRYIKITPRKDCNAV
                     RKEEKVLFPNYLFLHFDVNVTHTSAITSIPGAIGFVSFGSTICTVPEKVVIAIKCAKIL
                     ALNPDDDSIECRNIGSDLLAEIQRISSIISPLERQTAFSRLLQNQ"
                     /name="transcription termination/antitermination NusG
                     family protein"
     gene            12683..13051
                     /locus_tag="NML25_RS27140"
                     /old_locus_tag="NML25_27135"
     CDS             12683..13051
                     /locus_tag="NML25_RS27140"
                     /old_locus_tag="NML25_27135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248966.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_029397050.1"
                     /translation="MVPKRLKEAREAAGLSQEKLSELVGIEGTSTRSRMSNYEAGRFTP
                     PFKFICRVADVLGYPEYYFYIVNDVTAKKLLQMYKGEKNSIENIELEASRMADQLDDAR
                     KLVSQLSDCLRELKRSLG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            13700..14335
                     /locus_tag="NML25_RS27145"
                     /old_locus_tag="NML25_27140"
     CDS             13700..14335
                     /locus_tag="NML25_RS27145"
                     /old_locus_tag="NML25_27140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371526.1"
                     /GO_process="GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="WP_000088100.1"
                     /translation="MSRKRRRFLNRWIKSLTTVAGRIKIKNELRRRIRHNRYWVNEANK
                     FGIEALCELMLAILDDLDLRSWETRHNLETLAERAGLVTRSDAGHKSISRASRGCDRLV
                     WLNAIITEKAPFNPYDARCACKHIEVTEDFFAILGIPLKLVYRERARLLKADPDEVIYS
                     GDARLIAIRVQNWTRKAAAGLARMKAKRDAARQRKKEYYAHSPTPIPA"
                     /name="RepA family replication protein"
     gene            15294..15566
                     /locus_tag="NML25_RS27150"
                     /old_locus_tag="NML25_27145"
     CDS             15294..15566
                     /locus_tag="NML25_RS27150"
                     /old_locus_tag="NML25_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283543.1"
                     /translation="MSAFIFRTTFKATGDQQKDAITQIKVMQEQAVKAFVSWMTNQEGT
                     EFLSTLQEITTELDALIDAVESHSPVFRKKATELEALLAHYKTLL"
                     /name="hypothetical protein"
     gene            15640..15939
                     /locus_tag="NML25_RS27155"
                     /old_locus_tag="NML25_27150"
     CDS             15640..15939
                     /locus_tag="NML25_RS27155"
                     /old_locus_tag="NML25_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001615573.1"
                     /translation="MARLPYIESTTQQIFIFNTLTLCTIGLIHIETGKKYIVVFTDNNK
                     IRDYKNGVIEELGEITQEDIELIEYYAKKYEKYFDDLKIGEEPLNLKEYIECMQ"
                     /name="hypothetical protein"
     gene            16310..16843
                     /locus_tag="NML25_RS27160"
                     /old_locus_tag="NML25_27155"
     CDS             16310..16843
                     /locus_tag="NML25_RS27160"
                     /old_locus_tag="NML25_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025389.1"
                     /translation="MNIQEALNIFGLSGQLTEKDIKNAFKKAALKYHPDRNPVGAEMMK
                     AVNSAFDFLMANLDKINQYQSPEQSAIYNYGDELENVLKALYGLSGLVYEVIGNWIWIS
                     GNTIEHKEALKEIGCKWAAKKKQWFYRPEEHKSYWNREEHTIEEIRAKYGTSGKFQAGG
                     WQQVEGRARVEARA"
                     /name="J domain-containing protein"
     gene            16971..17513
                     /locus_tag="NML25_RS27165"
                     /old_locus_tag="NML25_27160"
     CDS             16971..17513
                     /locus_tag="NML25_RS27165"
                     /old_locus_tag="NML25_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283544.1"
                     /translation="MTTFSEKYQPVFEVVCRILGNGWRVNKLDNYQHRIKLTSTEIRKY
                     SIIIRLERERFFIVGSVDSLVGHGNPSVCTVSLTRNPVGIANDISRKILANALDEIELL
                     REHESALQEKQDKVRIIKGMLSQLFSISPWYGALTGFNADNGLAGKILQNGDDYEMVIR
                     GLNVDQLIKVAGFIKKL"
                     /name="hypothetical protein"
     gene            17550..17807
                     /locus_tag="NML25_RS27170"
                     /old_locus_tag="NML25_27165"
     CDS             17550..17807
                     /locus_tag="NML25_RS27170"
                     /old_locus_tag="NML25_27165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024192529.1"
                     /translation="MKTDNVFFKSKGSYSYVVTVDMDNQVYMVRKYVKTYVKSCDKEQV
                     KGKKTVKKLVLDTNLTLPELLSFDNGILADLFTRGNNNAN"
                     /name="hypothetical protein"
     gene            17797..18036
                     /locus_tag="NML25_RS27175"
                     /old_locus_tag="NML25_27170"
     CDS             17797..18036
                     /locus_tag="NML25_RS27175"
                     /old_locus_tag="NML25_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283545.1"
                     /translation="MQTKPTPADVKEARVSAGLTLKEAAEIFGYQLNTWQMKESAGKAT
                     RSLSVGEYNYLLLLAGKHPEYVLCKRDTVGTLNN"
                     /name="hypothetical protein"
     gene            complement(<18111..18221)
                     /locus_tag="NML25_RS27180"
                     /old_locus_tag="NML25_27175"
                     /pseudo=""
     CDS             complement(<18111..18221)
                     /locus_tag="NML25_RS27180"
                     /old_locus_tag="NML25_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /name="transcriptional regulator"
     gene            complement(18228..18527)
                     /locus_tag="NML25_RS27185"
                     /old_locus_tag="NML25_27180"
     CDS             complement(18228..18527)
                     /locus_tag="NML25_RS27185"
                     /old_locus_tag="NML25_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806992.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_094283546.1"
                     /translation="MGTYRELLAKESPEMQARVAERVEKASVQIALSALRDELNISQTE
                     LATMMGVKQPSVARMEQTDNDPRISTLKRYVKALGGELSLDVTLPTGKRVAFHL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(18529..18879)
                     /locus_tag="NML25_RS27190"
                     /old_locus_tag="NML25_27185"
     CDS             complement(18529..18879)
                     /locus_tag="NML25_RS27190"
                     /old_locus_tag="NML25_27185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006705960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094283547.1"
                     /translation="MWNVVTTDIFDAWFLTQNEDLRESVYEAMGLLEKFGPTLGRPYVD
                     TLNGSDFANMKELRVQYKGSPVRAFFAFDPTRNAIVLCAGDKTGLNEKRFYKDMIKLAD
                     SEYRKHLAKLEK"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            19191..19889
                     /locus_tag="NML25_RS27195"
                     /old_locus_tag="NML25_27190"
     CDS             19191..19889
                     /locus_tag="NML25_RS27195"
                     /old_locus_tag="NML25_27190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214917.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_249540332.1"
                     /translation="MDTIIIFFYKNIFIGGRMTVAIALVNGKGGVKKSSMSRAIATEYT
                     KADWKVLGLDMDVDQGSFLDWGQRRLRYKVEPPVTIQGCGTVSQAKKHIESGIWDLVVI
                     DSAAYATKSLLSLAEIVDLLVLPSGFSVDDLRTTVTTVNALRNKGHSTNKMAVVFVGVS
                     ESESEYRTALEYLKPCGVQVINGAIPFMTSFSQAQDKGLALTESPFPGPRQKADEVVQG
                     VIDLLTKLSQ"
                     /name="ParA family protein"
     gene            19929..20162
                     /locus_tag="NML25_RS27200"
                     /old_locus_tag="NML25_27195"
     CDS             19929..20162
                     /locus_tag="NML25_RS27200"
                     /old_locus_tag="NML25_27195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011998900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000024515.1"
                     /translation="MANVPPKPRNTKGEPPSLEDTQHGFNPTRKNADGRKAQLNLYVDM
                     EVKREFRSIAAANDMKHGELLEAYQEFWKKHH"
                     /name="hypothetical protein"
     gene            complement(20194..20457)
                     /locus_tag="NML25_RS27205"
                     /old_locus_tag="NML25_27200"
     CDS             complement(20194..20457)
                     /locus_tag="NML25_RS27205"
                     /old_locus_tag="NML25_27200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="WP_094283549.1"
                     /translation="MTIKILVVSNDGHEKLIDLSPVNDLAKITKSLRTSENRMVCVIQD
                     NNRILRWDRNYASRAKNHWRKVAPDRFEILGTIEHIHYVGKC"
                     /name="PilI type IV pilus biogenesis protein"
     gene            complement(20426..20803)
                     /locus_tag="NML25_RS27210"
                     /old_locus_tag="NML25_27205"
     CDS             complement(20426..20803)
                     /locus_tag="NML25_RS27210"
                     /old_locus_tag="NML25_27205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094283550.1"
                     /translation="MNDCFGCMHPYPAGHWFYSLQDLLTPLLENPLFYVFSFFFAGMIG
                     AIISRITKLIKTMKHSNNNNNKISIDNYDQIEIERYMTNEEVGRLVINFMKRTNGEPLL
                     IVFPEYDNGDDNDDKNTRCIK"
                     /name="hypothetical protein"
     gene            complement(20800..21279)
                     /locus_tag="NML25_RS27215"
                     /old_locus_tag="NML25_27210"
     CDS             complement(20800..21279)
                     /locus_tag="NML25_RS27215"
                     /old_locus_tag="NML25_27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="WP_094283551.1"
                     /translation="MKILIIFALYILSPSLYANAADIRGKVIRVLDGDTIEILQNKKPV
                     RIRLANIDAPEKKQAYGRWSTDQLKSLIAGQSVRVTYSHNDRYGRIIGRVFTTGGSEAN
                     RFMVQSGAAWVYEHYNTDNALADLQQEARSQKRGLWADPNPVPPWEWRHKYKVTK"
                     /name="thermonuclease family protein"
     gene            complement(21283..22017)
                     /gene="mobC"
                     /locus_tag="NML25_RS27220"
                     /old_locus_tag="NML25_27215"
     CDS             complement(21283..22017)
                     /gene="mobC"
                     /locus_tag="NML25_RS27220"
                     /old_locus_tag="NML25_27215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094283552.1"
                     /translation="MLIHDFYERKRISANNMLCLLNFLKDETYSDFHIIKRKLGFTKKS
                     RNPVYKLLNKALKEGFIIKHEIPSSKRKLTIWGITMQGLAMVVKADDITFPSYFEPSKL
                     KYWTLEHRLLNQRVRISLESKGATEWINGDSAAFIARYSGCSHRPDGVIKQPTGAIMAI
                     ETERTMKTRARYISIINSHLTARDKGNWHYALYVMPDTKSKESLIRLFDSIKTVMRNNT
                     PVPFDEKNREMFIFRTIDELEQ"
                     /name="mobC"
     gene            complement(22027..23754)
                     /locus_tag="NML25_RS27225"
                     /old_locus_tag="NML25_27220"
     CDS             complement(22027..23754)
                     /locus_tag="NML25_RS27225"
                     /old_locus_tag="NML25_27220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615560.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA transfer
                     family protein"
                     /protein_id="WP_094283553.1"
                     /translation="MNPHLMQKLNGIADAFSIFLLWLQNSPVILSLLAGLTLPFIFNLP
                     REERKNAPFWLKSVACVSIFFFIFGTMSPLSIHGLSYLFDVLDPNTLLRIPLWIITILF
                     TLAGLAFHLTARRLLAGEIDSLKHRMIKKSKLERNTRTDVRKVKELLPDSIEYNPLDYI
                     DLKKGVFIGLDKDDQPQYITIKEFQKQHAAIIGTTGSGKGITASVLLYQAILLGEAVFV
                     EDPKDDEWAVHVLREACKKAGKKFVVINLNRTNHQLDLLADISHEQLEELFNAGFSLSK
                     KGEAADFYRIADRRAARNVAAIHKTGMTLYDLFNDDNVKSIGEDAPAFLGELEEIALVN
                     SVNAKKGFSLKEIFDNGGCCYIIGSTRNQKIISAQRMILTRLIQIAETRDRINTTPRVV
                     AIFLDELKYHISRPTMEGLGTARDKSVHIFMAFQAIDDLRDCPSDLNGDSVIGAVIENA
                     KFKLIYKIQNPETAEWAAKMTGSILVDDEMRKVRTDLSLTEKVDTDRMIRQAESYYVDS
                     NMFLNLPERVGFVFTSKDLPKATKMSHIPANKETIELLSFETEQIEQHNNNDVPSSGL"
                     /name="type IV secretory system conjugative DNA transfer
                     family protein"
     Typing          complement(21287..22017)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.361"
                     /coverage="100.0"
                     /evalue="3.71e-162"
     Typing          complement(4806..5485)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="85.903"
                     /coverage="100.0"
                     /evalue="3.85e-119"
     Typing          complement(7711..10451)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="87.527"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcacgtggg cgtgcctttt ttctcgctgg ggcggaaaaa tttttgcggc tccccccggc
       61 ccgcttcaat tcgcttgggg cgaatgttcg cgggtgggcg tggtgcctga aaattaacaa
      121 tttcttcgaa attgaatttt ctgtccacgc accactttct ttattcctgt cttaacaatt
      181 tcttcgaaat tgaagccaga aataaagaaa gcctgaactg caccacgccc tgcgtcggac
      241 tcgcccaaat tcgcaaagcg aatttgcccc gcccttcggg ttgctacgca atcctctgcc
      301 tcagacttgt gcccgtgtta tttcagtcag acattcatcc tcggctttta ttacagcatt
      361 atatctatct tctgttattt cataaccttg tgaagcagcg taactaatca gcttattggc
      421 atcagaactt ttcagtttta ctgtgtgctc aattaaccct cgcattaacc tcgcttgata
      481 tatttcataa tatagtccgc atcccctgtt tgctttatta ctaatatcat caaagactct
      541 ttttacatgg attctgtcct gatttcttga atgaatatta ttaatgaaca tatcttcttt
      601 taacatagga ttctccagat ctgaaagaat atgttaattt ttcctctccg attgcaatgc
      661 taatgcttca gattctgtaa tgagaataat atcaggatgt cgtttcttta aatcttcaat
      721 agtttcacca atagaagtaa tgaattttcc tttcattcct gatggaatgg agtgtactat
      781 cttgtgtgca tagctataaa caagatcgga gtttgtcaga taaaatacca tgttttcatc
      841 tgtattcata tttatcctcg gactctacca aacttactca ttattttaga tatggctgcg
      901 ggatcagctg atgaacactg atttaaaaat gctttacgtg catcaaatgt tttactccat
      961 cgtattgaac ctttcttttt cttaagaatg ttgaagaatg ctttttctgc tgatctgcat
     1021 tcgccacctc cgctgtttcc tgtagctttt ccgtacatac ataaaacaac ctcgcatggg
     1081 tctgcggcat aagagtaaac aggtgtggcg gttgttaaaa caaacattgt tattgctgca
     1141 atttttttca tgtcagaacc ctttgtttaa tatatttgtg ttaacgcgaa aagtagatgg
     1201 ttgtttcatt gcacgtaaga caatttatga tggggtgata tataattacc ccgtttgcgc
     1261 catgatgata caaccagttt tttgtgctga aataatcagc gcctgacttt gttgctactg
     1321 tgatttgtgt tgagtgagca atggcataaa agacagatgg catccaatct tctttattga
     1381 aatcttgtga gctaatggat aacgtccatt tatcattaat aacaggagat tttattatta
     1441 agctattggg tttccattca ggaagtttat cgttaagcga taccggcttt ttattccatt
     1501 ccacgggtac tggtggaggc ggtgaggaac atccggtaat tagtgttaca aacacagtaa
     1561 ccagtatatg ctttatcata ctttcatttc ctcgtaatat tgattgcggt gtaacggtcg
     1621 gaaataaata tcattcatac ggcgtatgtt tccatctaaa tcaatgctca caacaacatc
     1681 cagagagtcg agaactttac gtagcaagaa tgaatatggt aattgcgcac actgtgggtt
     1741 ttcagtacag cgggtaataa aaccgtcgat tgcttcatgt gctgaaccag catgaatgga
     1801 ggtcatactt ccttcatggc ctgaccccgt tattttcaga aaatcccatg cctctccacc
     1861 cctcacttct gttaataaaa ttctgtctgg attcatccgg taattccagc gtataagcgc
     1921 tgctggtgtt acaacagatc ctttttcatc ggatgattca gaaggataaa acagatgaac
     1981 atagttctta tgtttgtaga atattatctc tgggttgtct tctatcgtag ttatgcgtaa
     2041 atggacaggt atatattgta tcaacatttt catgtatgtt gttttacccg tactggtttc
     2101 acctgcgacg gccaatgttt ttccatactc cacgcatttt tccataaaca aaggaatgtt
     2161 ttttgatttg tataattcaa tcagttcttc gttatgtgtg gatgcttttt caatgcccgt
     2221 gacgcggtta taaaaaccgc tatcaatata tgcctgatgc ggaatttgaa tttttgacgg
     2281 tttacggatc gtgattgaga tcgtgtcacg ttcgcacgcc ggaggaacga caacctgaca
     2341 tcgttctcct gattcaaggg ttgcagaaag tcctggtttg atatcctcga tattatcgtt
     2401 atggtgtttt gcaagacacc gtgcaaagtt atagcaatcg tcaaatttca gatcaacggt
     2461 atgttgcccc cagacgccat ctatctttgt ccagagttca cctggacgat taacagctat
     2521 ttctgtcagt cctggtaatt ccaggtattc actaaaaaaa cgttgtttat agcgatcaag
     2581 agatatgttt ttaaccgtca tgttctattt catcctcagc gtgtagatat ttgagaagtc
     2641 aatatcctgc cctgtaatta ggttgattat ttctccctga ttcttgtaaa gcgttggtgg
     2701 aatattaatg ctgttttcca gcgtggttcg agccatatcc gccatagcct ggcggctgtt
     2761 ctctgtatag tctgtattgc ggtctttttt acccgcattg tttgatgccc atgcgccaat
     2821 atctgggatc atgccgacca ttaatgcgcc gccaaacctt tcccagaagt gggtatcaat
     2881 ccatccatca acacctgatt ctccaagctc gccagcggcg ttggtatcaa tcagcggaat
     2941 atccagatat ggtcgctgtc gggtacgtaa ttttgtcatc atgataaaag cgcgcccttg
     3001 cccttgcgta attcccgtct ggtatattcc tgttgccgtg gttcccttct cgataagttt
     3061 tgtattgccg ctggcactcc agatatcttt agctaccgta catcgtattc ttccagcgcg
     3121 atcggaaaca aagcggtaat ccatagagca tggaatgact gtgttttccg gtatgtacaa
     3181 atcagggttg taggggattc ttcgcactgt cgcgggggaa attgcctgtg atgcagtgcc
     3241 atgttgtgtt gtggttgctg tactatctgt aggttgtgtg gcgttgtttt ccgcagtcag
     3301 tatcatttcc tgttgtcttg ttttgacctg attttggggg ctggccgtat tattacgacg
     3361 aacaaggaaa ctggccttat tcagttgtgg tggctcaaat gttttcatct gtccggcagt
     3421 accggctaca ttgcccgtgt tatcctggtt aacagtttgc tctccctcag tcgaattttc
     3481 cggctcattg gtggccatgc ctaaatcggt tcgtttccgg tagtcattcg tttttttagg
     3541 tggcgtaacc tcctttgttt tctctccatc ttcttgcgtc aggaagtttt gatataccca
     3601 gttaccgccc catgtcaggc caatcagggc tactgcacaa attgccagaa aggccgttgc
     3661 tttccccttc ggacgttttt tcagtttgtt tactgctggt tgtaatgagt tctccttttc
     3721 atcgtcctgt aatgcttgta attcagcctc acgttttgct ctggcttctc gttccagcgt
     3781 ggcgatatca tcctgatcag gtgtcgtcac tggcttttct tcttccgtag agggggattt
     3841 attttcgtca gtcatatatg agtccttata taaaaatata attatatttt tatagattag
     3901 atgttgtctg ttttttcaac gcgctccacc ttgtcagata cggttgaatt atcagtcgcg
     3961 tgtacttttc caaagccgtt atttttcagc cccactacgg catcacccga acgtagtaca
     4021 agccgtggtg tgatttcctg gataacgaga acggtaaaat tgcccttacg ctgtatactg
     4081 ctgttaacca cgtattcttg gccgttctgt tctttggtaa ctgatggaac tgatttgttg
     4141 ggtgagaatc cgataaacgt aaagcgaccg tcgtcgtagg caaaatcagg ttcaatgctg
     4201 taactgtctt ttcctggata tttcgtgtag tcccagttac gtggggtttg tgcggttctc
     4261 agtgctttct gaatctgggc ttgttcctgc ttttgttccc attctttctg acgtgctgct
     4321 gcttccttat tggctctgtt acgttcgtcc cctggatagc ggtaactcac caggtatgcg
     4381 ggctgttgtg gcgatttttc atcaatgaca ttcagttcaa cgttgtacag gcgcttattt
     4441 gtcacaatca gcatattggt atgccagtca cggctgttcg gcgggataac aacctgtgtt
     4501 gtattaccgt cttcaccagg tgcgccctgt gtgagcgcaa ccggacgaat gttaaccctg
     4561 tttgcatctg ctctggcttc ccatgcttca tcaaagccag gactggcact gataacagct
     4621 tcatcgttat caaaaaccag agtcgtcata aatccaggtt ttgtgtttac aacggtaaca
     4681 ttctgcgggt tataaatgac ctgctggata cgtgcatcat aacggctcgc ctgtggcgta
     4741 gctgcggccc atgcctggct gttcagcatt attatcaggg taattaacgt cagttttctc
     4801 attactcgcc tcccctgatt tcgcggtcag tctgccagct tgttacggtg aatccgaacg
     4861 ggtttatttc ccgctcaaca tctgacattt ctttttcagg gtctgaatgg aaggtgaaac
     4921 gtgcatccca gtattcattg cgaatactgt tatcagccat gcgacgaatt atttttttat
     4981 aacgaacggt ggctaccttg tctggtgctg ttgcattcgt tatctgctca gaaataattt
     5041 caattttcac ggtgtgagcc gcgttattat aaactttgtc tggtgcgttt tttccggcgt
     5101 acagtgccag gtagtcgtcg tttacctgtg gtgagttgta gatctgtact gtctcgtaat
     5161 cgtcctgtaa tgcaggataa acgtaccgtt cacgtaattt aacgtaattg gcggccatag
     5221 ctttttggta ggcttcttcc gctgtcaggg ctgtttttga tgcacgggta acgtgttcca
     5281 ctcgtccggt atgattatct acggtataga gttcaactgt tgtttgttta agtggcaaca
     5341 taatcactat cgcgatcagt gacaggatag cgattaataa ccctgctcca ccgaccagaa
     5401 agcctgcctt tgtggcgcgt ttatccctgt caatcatttg ctgctcaaac gagcgggaat
     5461 cagcaattaa tttctgttca gtcatgtgtg gttagctccg cgataatttg gggtgtgttt
     5521 acagggctac cctgaccgct gaatgcgggc ggcagagatt tagatttttg tgcgcagcca
     5581 gaaagcacgc acagcaaagc cagcgcgatt aagcgtttca ttttatgtac ctttttgttt
     5641 ctataaaaat ataattatat ttttatagtt atcttggagg gggtaaacgt ttaattgatg
     5701 gcggtagttt tggtgctgat tgatctgtca gcatccgcgc ttcattacgc gctatcattt
     5761 gttctatagc gtgtttacgt gcatcagcag cgttaaatac tatgcgcgat tgtggatctc
     5821 ctaacccttt attacttggg cttgctactt ctttaggtaa tacttttcct gcctccttta
     5881 acatctttcc agcgccgtat gcagcagcgc caatgccgag agccgccgca ccttgcatag
     5941 tggctgtagc acttgctcct gcaagtgccc cagccagttt tgcggacatg tacaccagta
     6001 ttgcagcgca tactccggca agacaaactt gagcaccaag ttctacaatg tttgctttgc
     6061 tggcctgggt cactgcttct gacattctgg atgtaagaaa accaattaca attcgcagag
     6121 ctaaggctga aaacataatt gttagtattg cagcaaatat attttgaagc cagttattaa
     6181 acattggccg taaaaagcca tacatcaggc aaaatataaa tattggtgcg gtaataccta
     6241 acaataagat catgatctcc gcaatcatag atacgaatgc tgtgataatc attaagacaa
     6301 agacaccacc ccatacaaat aattcagcaa ttgtgccttc ctctttgata aattcggagt
     6361 tgtctagatc atgtaacgtt ttacctaaaa ctcttgcgct gttccataga gtatcaagta
     6421 attgccagat gcttgtgcta ctggaaaaac cttcttttat tccatttaca gcatcaatga
     6481 caccatcaag atatccgtta atgtttgcta caaaggataa aatgatagcc atcctcatta
     6541 tatcccacat aacatcttcc attggtgtag acagcttccc cgctaaggtt tgatatcctc
     6601 gccacataat gtagatagtg gctgacgtag ccgccagccc catcatcatt gacgaataag
     6661 acatcatcaa accattagtc gcgtcagtta tcccaccgat gatatttttt tcgacagaag
     6721 aaaatatacc ggccatgata aacgcccttt agttgaaact tatttcaggt gcatttaatt
     6781 gttgaatttg ccagtgagct tcattggcat gtttgcagtt ttcagatgcg ctgcctgttt
     6841 tctggcattc agcataaaca actttgagtt catcaggatg ttcacgatac cattttgttg
     6901 ttttagcttc ttccttgcat cctgatgtta acgccatcag gcctgaaata attgtaaata
     6961 aaatgatttt cttcattaag tcacctgcta attaaacatt atttctggtg cgtttaattg
     7021 ctgctgattc catttcttac ggcgttcctc agcaagcatc ttttcacgct tctccgccgc
     7081 ttttacgttc atttcccatt gggtcgtgag cgtatttagc atcacagatt ttaactgaat
     7141 actgtttgct aagtcttggg cctctttcat atctttggca tttgctacac ggtctgataa
     7201 ttcgttgatt tcaccaagca tctcaccaat ctgggtttgt acttcattgg tttgttcgat
     7261 ttgtattgct ttgttgatga cctcttgtct gcatgtattg gcataacttt ttgattgttc
     7321 agcattgcag ttatcgaaaa tcttgtattt gttgtacagt tcatcaaatt ggccagaaga
     7381 accgccggat aagagcaaat cgttcagggc ttgccctggc ttacgtagtt tatccaggtc
     7441 agctttaagg cttttagcct gattaacgaa tgcctgaatg tcacggatgc ctgtaactgt
     7501 ttccagttgt ttttgatacg cctgtagctg gtctttatag tgttgtaccg tctgtagcca
     7561 ttgattggct tcttttgtcc actggcgaat attctccagt ctggctgttg catcaaacac
     7621 gggtatacca gcagcgttta ctatcgggct ggcaataaga accgccagaa taagggggcg
     7681 aaatcttttc atcgatatct ccttggttat agtgctttag ccaggtagga atccagccag
     7741 tcgctgggct tcattccctc ctgatagagt gattcgaata tttccaggtt gggtgcatcg
     7801 ccgctcatta cttttgtgta tctgccgata ccagacaggt ccagcgtgat acgggctgca
     7861 aagcgttttg tatctccacg gcggaactga tttttgacaa cgacatactg ccgcgcctgt
     7921 ggatcaagtc ctttaatgac atcaaacact tctggttcaa atttcattcc atccacgtaa
     7981 tgagagcgat ctgcgcttgg gttggcggcc agtatttgag taccacattg ctcaataact
     8041 gccggagcta tctcatcctt gattatttcc gctggcgatt gcgtgccaac aaccagcata
     8101 ccgttcagtt ttcggatagt tttcagcttg ttatacgcaa aatctttaaa tacagggtcg
     8161 cgtaaccact tccagaattc atccatgaaa ataacaagtc gtctgccatc cagcaggctg
     8221 gttatccggt acagcagata aaacgagata ggagcgcaga cattcgggtc atccagaaat
     8281 tcagttccgt caattccgaa attatcgcag ttgctgatat cgaaagtgtc gtgttcgtta
     8341 tcaaacaccc aaccaaattc accaccgcgc gcccactgtg aaagacggat actcaggccg
     8401 ttttcttgcg cttcacgcgt tgccggttcc ggtaggttct ccagcattcg ggttatgccg
     8461 tagacgcgat attgaggttc atcactcatg acggcattta cagcatcatt gaggcgacgt
     8521 tcatcacgtg gcgttatggt tgcaccgttg cgggtgcaaa ggattttcat cagttgctta
     8581 atgaaattga tgtttcgctt agtggctggc agagcaaacg ggttccagcc tgttggctca
     8641 ccgctgaata ccctgtagta ctttcctcca agtgcgcgaa tattgatttc agcaccacgg
     8701 tctttatcga ggtaaactgt ggttagtcgt ttagtcctgg catccggtga aaagctgtca
     8761 tcacggttat atttttgctg attgatttgt aaaaacgtca tcagcattgt tttacctgaa
     8821 ccgtttgtac ccagaatgca ggtacttgca gggttttttt cgttgaactc gtctttatct
     8881 gccagagtgt tatgcagatt cagatagtag ccatcacctg acggggttcg caccagtgcc
     8941 atcgcgtcac cccacggggc tttgtcgcgt tttcctggat agaaattatg aagtgatgcc
     9001 agttcaacaa agttcaggct actcagctct ccggctcgtg gccgcagatt gtaattgcct
     9061 ggtaattgag ccatataggc cgcagacaat gagatttcag caggaacagg ggttatcccg
     9121 atattgttaa gtgcattgct gatctcgttt gtgtctgcaa tcaggctttc cagtgacggc
     9181 gaaaacacca taattgataa atgatgttta ccgtaggcga ttttaccgga ggtgagcagg
     9241 tcaagcgcca catcgagatc caaacgttga gatacagcat catcatcagc actcatcatc
     9301 agccgtcggg tgcgcttaat cgcctttttc gcttcctcac gggacatgca ggtataggat
     9361 tgtgtaatca cataatcgca cggcgcgtac agtaaggagt ctgtcatgcc cgttgcggtt
     9421 tcttccgaaa actctttaac ctccagacca cggaaatatt gagtgccatt aacatgattt
     9481 atttgccctg attcggcgga gaagtacagc gcaacatttc ccattacgtt gtaagcgggg
     9541 gtacgtgtaa ctctgacttt tcgccattga tgcgtgagaa ggtattcata aaatgccagt
     9601 tgggacgaga atacagcctc gttttcggtg aaagtaccga gcgcgtgagc accataacgg
     9661 gataaggctg tatccagcgt acttttgatt tccagcactt caagtaatgc gctgtctatc
     9721 tcttttagct tctgaccgtc tttcatacgt ttacggtttg ccttatccag actggcaaat
     9781 ggacgatagc agatggtcac aaacaggcgg ttacgtcgga atggcctttt attcagtgat
     9841 gcgtaatagc gttcgctcac ggtatccgcg taagggtttc ctgaaacctt atgcagtgag
     9901 tccgtaaact gttcacggct gttatgtaca taaaatgtaa ccggaaagcc ttcaaatgag
     9961 cgtattagcc cgtgcaactg gtttgtaccg aactgtagtg tctcgtcaga ttcacaatca
    10021 aacggcgttc ctgtcagctc ccaggtacag aaaaaatcgc tattcagtga acggacaaca
    10081 ttagggtgaa tatgtgacga gtaaggaatg tattttttta tggtggcaca ttggtttagc
    10141 ttcatcgcat cgataaactc ctggatatca acagcattat gttcaacagg cataaatgcg
    10201 gttgcgccaa ataaacggtt tgctaccgga ttacccctta cccttagcca gacagctatc
    10261 aggttaaatc tcagaggttc atttctggtt agtgatttca taaccagaaa ctcaataggg
    10321 aggaagagaa gtagggctat ggaagtccag atagcaacaa taattgtcac gcccacgaca
    10381 gtcagcaggg gcaccattgg caccccccac agcgccgccg gacgtttcat cgccttaagc
    10441 agctttgcca tatgttcggc ctcttagctc attagcagag cgccaatttc actgcctccg
    10501 ccaattaaaa tgccgccaat gatgatgtaa ccgcactgcg acaggctttc gcctttccac
    10561 agagtcttat agccaatcca gatcacagcg acggtgatgg tgacggctgc cagaccatgc
    10621 agaccattcg aaaccttttc catgatggta tttgctcggc tgaaaccgtc agccgcgaaa
    10681 actggactga caacacttac taacggagcc agaatataag cagatgcgcg gctgcaaatg
    10741 gcctttaatt tgttaaacat cggttaatcc tctttttcag ttgaaaatct gtttccgcgt
    10801 ataacttgtg cggggtaata aacggtatct gcctttttct ggcgcagttg agcaggaacc
    10861 ccgcgcacga ttgcgccagg ccagacaacg cgagggcgtg ccggagcttc aacgggtgtt
    10921 gcattcagaa tggccgattg agttgcgata tcatccttgg ttccaggaac gacatagcgg
    10981 ggcttttcag gtgagtaacc aattcgctgt atgtaactgg ttctggataa cgcagcttct
    11041 ggttgttgtc ctgtagtaaa atttccagag taataacaac tcagcgcccg tttcagtgtg
    11101 ccgcctcgct ggtagcaatc agtgatgatc ttttcaaaaa cagaaagatt tgtgcacggg
    11161 ttgaacaggt cagcagccgt tacggcgtaa ctattaaaat ttgtacttgt gatttgcatc
    11221 agaccaacag aatagcggcg accttttgcc tctatctgtt tgacgatgct taatgcatcc
    11281 tccttagttt ttggcatatg tgagataaag cccttgtcgc ctggctggcg ttcacgtttg
    11341 gggatgatct cagcaatagc gtagggatta aaaccagact cgacccgcgc aacatccagt
    11401 gatgtggagg ggtgaactgt ggcggcacac tgcatagcca ccgccataaa tgaaacggtg
    11461 gaaagcatag taccctcgct aaattagttt ccttgtgggg tgtcttcttt aggttcgagt
    11521 agtaccagct tgccggaaac agcaatacca ggaacgagca taatattcag tcctggtttt
    11581 ccgttatgcg cgaatgccgc gccaaccttg gtccagtagg tatttttacc gccgttttcg
    11641 tcagtgggaa cttcctgtgt gacgtatacg ttataggtgg gtttgggcat ttatttatcc
    11701 tcttttgaca ttagctcttt caacatttcg ttgtgagctt cattacttaa tttgtcgatc
    11761 atgtactgca aaaaggcact tggacgaata tttacctccg caacataact tatatctctg
    11821 gcgtgttttg ccagtgtttc atagcgtttt ttgccgatgt aaataacggc tgtagattta
    11881 gcctggccta tagggttcgg catgagttta tagctcctgt atgaaatatc cgtgtgcaaa
    11941 agcacagcgg tacgactgga gaaggaatga aggtctggtt tactgattct gtaaaagcct
    12001 ggagaaggcc gtttgacgtt ctaacggtga gatgattgaa gatatgcgtt gaatttctgc
    12061 cagtaggtca ctaccgatgt tacggcactc tatggaatca tcatcaggat ttagcgccag
    12121 tatcttggcg catttaatag cgataacgac tttttccggt acagtacata ttgtggaacc
    12181 gaaacttacg aagccaattg cacctggaat agaggttatt gctgatgtgt gcgtgacatt
    12241 cacatcaaaa tggaggaaga ggtagttagg aaataaaact ttttcttcct tgcgtactgc
    12301 gttgcaatct ttccgaggtg tgatctttat gtatctcgga cagaaagcca caacgttcat
    12361 agttttacaa cgatatgcaa tgcggtctaa ctcgttgtta ttacaatata aaagatacca
    12421 tgcattcata agcgtcacct tacggacaca agcccccatt ttccgccgtc ctaagttgcc
    12481 gggcttagga cggcgtactc attaagtgtt aactgtaaaa atacacagtt gtgattttat
    12541 acagctattc ttgcgtgtca atatcaagaa tctaaagaaa taataacaag tgtgttcatt
    12601 aagtcaaagg ttttggatgt atgaacattc aaaaattggt gccgatgtat gttttggaaa
    12661 acaaaacaag gggatcgggt caatggtgcc aaaacgattg aaagaagcgc gggaagctgc
    12721 tggtttatct caggaaaagc tatcagagtt agtggggata gaagggacta gcacaagatc
    12781 taggatgtct aattatgagg cgggacggtt tacgccacca tttaaattta tctgtcgggt
    12841 ggccgatgtc ttaggatatc cagaatatta tttctatata gtgaatgatg taactgctaa
    12901 aaagttactt cagatgtaca agggagaaaa aaacagcatt gaaaacattg agttggaggc
    12961 aagtagaatg gctgatcaac ttgatgatgc tagaaaacta gtatctcaac ttagcgactg
    13021 tttgagagag cttaaacgtt cgttagggta agtttcgggc atcagtgcgt atgtgctcca
    13081 tttggtcaag tcgcttttcc gcttcctcac ccactcgctc actaacgttc ggtcgctagg
    13141 gttgtggcga gcggtaaata ccctattaaa ttccgttatt tttcggtttg ttatcgccga
    13201 tttcttttcg atattaatgc taatgtatgc gtacattagc attaatatcg tgagttatat
    13261 gctgtacggt atttaatctc aattttgtta agaattatat aaccagatga agtttaaaga
    13321 aaaataggaa ggtaatcggc gggatctttt gtggatgaat tgatcttcga atgggttcat
    13381 gctattttcg tgcggttttt acggattttt gggtgggacg ttcccgtaac agcgtccaag
    13441 ggaagggggg ggaagcattt tagccccccc ttgaatgcaa caaccccctg atatctctct
    13501 tcgattttgc cggtccaaga agataatcag ggggtccaaa gcaggctggg tgcctgcaaa
    13561 ggattatagc aaatgcattt ctcaatacaa gacctctgtc cggcaataag tgcgggcggt
    13621 ggtggctgaa aagaacaact ccttaatccc ccgcatatcg ccgtcagggg tggcaaaaca
    13681 aagtccatgc tataacgttg tgtcgcgtaa gcgacgccgt ttcctcaatc gctggattaa
    13741 atcgctgacg acggtcgcag ggcgtatcaa aatcaaaaac gaactccgcc gccgtatccg
    13801 gcataaccgc tattgggtta atgaggctaa caagtttggc atagaggctc tttgcgaatt
    13861 gatgttagct attctggacg atctcgattt acgctcgtgg gaaaccaggc ataaccttga
    13921 aacgcttgcc gaacgtgccg gactggttac acgcagcgat gcgggacata aatccatttc
    13981 tcgcgccagt cgtggttgtg atcgcctggt ttggttgaat gccatcatta cagagaaggc
    14041 tccgtttaac ccgtatgatg cacggtgtgc ctgtaaacat attgaagtaa ccgaggattt
    14101 ctttgccatc cttggcattc cgttaaaact ggtttatcgg gaacgagcca ggctgttaaa
    14161 ggcagatcct gatgaagtga tttactccgg tgatgccaga cttatagcta tcagagtcca
    14221 gaactggacg cggaaagcgg cagccggatt agccaggatg aaagcaaaac gcgacgcagc
    14281 tcgacaacgc aaaaaagaat attacgcaca ttcacctacc cccattccgg cctgatgctg
    14341 gctacttacc gccttatggg cggtatatcg tcacgtccat aataaatctc tgaacaaaaa
    14401 atagtcttag cgtgagatat tttccgtttt ccaagcggct ccctgcttag gaaagagcag
    14461 ccataggtta tggccagagg gtaaacgatg tacagcttgt ggataatcat aagtgaagtg
    14521 caaacgatat gacgcaatta ctcttttgct tctcttttag ttacttacgt actaaaagag
    14581 aagcaaaaag aaaaacatta ctgttatccc cttttacaag tggataactg aaaagcatcg
    14641 gcgcaagcgc ctcaaaggta gcacccctcg caagcatcgg ggcattagag acaccatatc
    14701 cggtcattaa aaacgaacaa tcattcacct atcatcgctg taacatcgac ggtctacgac
    14761 cacctaccac ctaagtagct gtaagttccg tgcaattttg gcttagcagg gggctgacgc
    14821 ggttcctggc gacatgaacg tccttgatta ggggtatcgg cgcgccttag catttgtgat
    14881 tctatggaac tgtatttcgc gccttacatg ccgctaacgc ggcatcttcc caccatcgag
    14941 cataacttaa ctaactgcta acaatatgat cgagaaagat cacaaaaagg ttcctgttgg
    15001 aggtcagttt ggaaatcgga aatttcggcg tttccggtgt acaaccctag gaaagtagat
    15061 ccgctcgccg caaccctagc gaccgaacgt tagtgagcga gtgggtgagg aagcggaata
    15121 gtgcccttgc tgaattgagc acatacgcga aggaattaaa ggtgagtggt ggaaaatttt
    15181 tcagttcgaa tagtgaaaaa tagttgcaat acctgcatat gcaggtataa taaaatcatc
    15241 gaaagggaaa taggttccct ttccacttag ccccataggg gcgggagatt aaaatgagtg
    15301 catttatttt tagaaccaca tttaaagcaa ctggcgatca gcaaaaagac gctattaccc
    15361 aaattaaagt tatgcaggaa caagcagtaa aagcctttgt ttcctggatg accaaccaag
    15421 agggtacaga atttctaagc actttgcagg aaatcacaac agaacttgac gcactgattg
    15481 atgcggtaga gtcccattcc cctgttttca gaaagaaagc gactgaactg gaagcgcttt
    15541 tagcccatta caaaacgttg ctgtaagtcc tcaacggggc aattgccccg ttatatggct
    15601 ctttatttaa tccaaatcta aagcaagtgg agtttaaaaa tggctcgctt accttatatt
    15661 gaatcaacca cgcaacagat ttttattttt aatactttga cactttgtac tattggatta
    15721 attcatatag agacaggtaa aaaatatatt gtagtattta cagataacaa taagattaga
    15781 gattataaaa atggtgtaat agaagaatta ggggaaatta cgcaagaaga tattgaactg
    15841 attgagtatt atgctaaaaa atatgaaaag tattttgatg atttaaaaat aggtgaggag
    15901 cctttgaatc ttaaagaata tatagaatgt atgcaatagc catatattct ataaaggtgt
    15961 gatcaggttg gtcgagcaac ctgatcactt tatcgacact caaaacaata gagaggtgtt
    16021 caaatgtcaa caggtcagat cgtacaactt gcggattcac ttgtcaaagg atgggaaacg
    16081 agaatcccag caatgaaatt tacggattgg gatcaattca ggtggtggtt aaattattta
    16141 cagggatatg aaatgtttta agcaatgttg atgtcctgac agtaattata aattttttca
    16201 aaaagagagc taaaaaactt gcaatacctg caattgaagg tataattaaa tcatcgagag
    16261 ggaaatggtt tccctctcat cttagcccca caggggcggg agctacaaaa tgaatatcca
    16321 agaagcatta aatatttttg gcttgtctgg ccagttgact gaaaaagaca tcaaaaacgc
    16381 ctttaagaaa gctgcattaa aatatcaccc agaccgtaat ccggtaggtg ctgaaatgat
    16441 gaaggcggtt aattcagcat tcgatttttt aatggcgaat cttgataaga ttaatcagta
    16501 ccaaagtcct gaacaaagtg caatatataa ctatggtgat gaactagaaa atgttttaaa
    16561 ggctctttac ggtttgtctg gtttagttta tgaggttatt ggtaactgga tctggattag
    16621 tggaaatact atcgagcaca aagaagcatt aaaagaaata gggtgtaaat gggcggcaaa
    16681 gaaaaaacaa tggttttatc gtccagaaga acataaaagt tactggaacc gtgaagagca
    16741 cacgatagag gaaatccgcg caaaatatgg cacaagcgga aaattccagg ccggaggatg
    16801 gcaacaagtt gaaggcaggg caagagttga agccagagca taacgaaacg ggggcgtaag
    16861 ccccctatat cctcgccagc aatccttttc gggcatcgaa cccgaaaagg attgagtagc
    16921 tgacactctg cgattgctgg ctacgttagc gcagtaaagg atgtgaaagc atgacaacat
    16981 tttcagaaaa atatcagcct gtctttgaag ttgtatgccg tattcttggg aacggctggc
    17041 gtgttaacaa actggataat tatcagcatc gtattaaact gacttctaca gaaattcgta
    17101 aatactccat cattattcgt ctggagcgtg aaagattttt tatagttggt agcgttgata
    17161 gtcttgtagg gcacggtaat ccttccgtct gtactgtatc tcttacgcgc aatcctgttg
    17221 gtattgctaa tgatatatct cggaaaatcc ttgctaatgc actggatgaa attgaattgc
    17281 tcagggagca tgagagcgca ttacaggaaa aacaagataa agtgcgaata atcaaaggga
    17341 tgttatcaca gttgttttct atatctccct ggtatggggc attaacggga tttaatgcgg
    17401 ataatggact tgcaggaaaa atactacaaa atggtgatga ttatgaaatg gttatcagag
    17461 ggcttaatgt tgatcaactt atcaaagtgg caggttttat aaaaaaacta taacaaagtg
    17521 ttgatgtgaa tttttatatg gggttgttta tgaaaactga taatgttttc tttaaaagta
    17581 agggtagtta ctcttatgtt gttacagtag atatggataa tcaggtgtat atggtcagaa
    17641 agtatgttaa gacatatgtg aaatcttgtg ataaagagca agttaaaggg aaaaagacag
    17701 ttaaaaaatt ggtgcttgat acgaatctta cattgccaga attattgagc tttgataatg
    17761 gtattctggc tgatttattt acaagaggta ataacaatgc aaactaaacc aactccggca
    17821 gatgttaaag aagcgagagt ttccgcaggt ttaacgctta aggaagccgc cgaaattttc
    17881 ggctatcagt taaatacctg gcaaatgaaa gaaagcgcag gtaaagctac aaggtcttta
    17941 tctgtaggtg aatataacta cttattgttg ttggctggaa aacaccctga atatgttctt
    18001 tgtaaaaggg atactgtggg tactttaaat aattaaggat aatacaatta attcgccgaa
    18061 aatgaacttt tcgcccattt tcggctttca ctcctaaaaa gctgcaatta ttcacgcatc
    18121 atttgcagtc ctgtttccag aatcatctca ttagccatgg ctttgatgat agcctgactt
    18181 tcaggtgatt gagccgcaat tacttcttct aatgttctca tctgttttca caggtggaaa
    18241 gcaactcgct ttcctgtagg caatgttacg tccagactta actcgccgcc taacgctttc
    18301 acataacgtt taagcgttga tattctcgga tcgttgtcag tttgctccat cctggcaact
    18361 gatggttgtt ttactcccat cattgtagcc agttcggttt gggatatgtt cagttcatca
    18421 cgtaaagcac taagtgcgat ctgaacgctg gctttttcaa cccgttcggc aacgcgcgcc
    18481 tgcatttcag gactctcttt agccagtagt tctctatagg tgcccatgtt atttctccag
    18541 ttttgcaaga tgtttacggt attcggaatc agccagtttt atcatgtcct tatagaagcg
    18601 cttctcgttc aatcctgttt tatctcctgc acaaagcact atggcgttac gggtaggatc
    18661 aaatgcaaaa aatgctctga cagggctacc tttatactga acgcggagtt ccttcatatt
    18721 ggcaaaatca gaaccattca atgtatctac atatggcctt cccagcgttg gaccaaattt
    18781 ttccagtaat cccatagcct catagactga ttcacgaaga tcttcatttt gcgtcaaaaa
    18841 ccacgcatca aagatatctg tagttactac gttccacata aaaaacctat agcttattca
    18901 ttatatctaa tattataacg aatacactat attttcaata aaaacgaggc tatttaacat
    18961 aatggacgtt atcggcacca tcggatcacc gttcgttgtc ggatctgcaa aatccgctca
    19021 aaagatcatg ttttcctggg cgttttccac cgttttttgt gttgcgtatt tgttaataaa
    19081 acgtcatttt ttgttttgcc tgaatttggt tgtgaaagcc ttatattcac aagttatctc
    19141 ttcacttgcc gcctgtagtt caaatagttg ttgcaattct tggcaccaat atggatacta
    19201 taattatatt tttctataaa aatattttta tagggggtcg tatgacggta gctattgcgc
    19261 tggttaacgg taaaggcggg gtgaaaaagt cctcaatgag cagggctatt gccacagagt
    19321 acacgaaggc agactggaag gtattagggc ttgatatgga tgttgatcag gggtcattcc
    19381 tggattgggg acagcggcga ttgagatata aagttgaacc acctgtcact attcagggat
    19441 gcggaacggt atcacaggct aaaaaacata ttgaatcagg gatatgggat ttagtcgtta
    19501 tcgatagtgc tgcttatgca actaaatcgc tgttgagcct ggcagaaatt gttgatttgc
    19561 tcgtgttacc gtctggtttt agtgtggacg atttgagaac gacggttaca accgtgaatg
    19621 cattgcgtaa caagggacac tcaacaaata agatggctgt agtttttgtc ggagtcagtg
    19681 aaagcgaaag tgagtatcgt accgcgcttg aatatcttaa accgtgtggt gttcaggtta
    19741 tcaatggcgc aatacctttt atgacctcgt tcagccaggc acaggataaa ggactggcat
    19801 tgacggaatc cccttttcca ggtccacggc agaaagctga cgaagttgtc cagggagtta
    19861 ttgacttgct gactaaactc tcacagtgat atataattct ataattatat ttttatagag
    19921 gtgataagat ggctaatgta ccaccaaaac cgcgtaacac caaaggcgag ccgccaagtc
    19981 tggaggatac acaacacgga tttaatccga cgcgtaaaaa cgctgatggg agaaaagctc
    20041 agcttaacct ttatgtggat atggaagtta aacgggagtt ccgctcgatt gcagcggcta
    20101 atgatatgaa gcacggggag ttgctggagg cgtaccagga attctggaaa aagcatcatt
    20161 aaaatggcat aggggacgaa ctgtcccctg atattaacac ttaccaacat aatgaatatg
    20221 ttcaatggtt cctaaaatct caaaacggtc gggagctacc tttcgccagt gattttttgc
    20281 acgagaggca tagtttctgt cccagcggag aatacgatta ttgtcctgga taacacatac
    20341 catcctgttt tctgatgtac gcaatgattt tgtaatttta gctaaatcat taactggact
    20401 tagatcaatt agtttttcat ggccgtcatt tgatacaacg agtattttta tcgtcattat
    20461 cgtctccatt gtcatattct gggaacacta ttagtaacgg ttctccgttg gtgcgtttca
    20521 taaaattgat tactaatctg ccaacctctt catttgtcat atagcgttct atttcaattt
    20581 ggtcataatt atctattgat attttattgt tattgttatt gctatgtttc atcgttttaa
    20641 ttaatttggt tattcttgat ataattgcac ctatcatacc cgcgaaaaaa aaggaaaaga
    20701 cgtagaaaag aggattttcc agtagtggag taagtaaatc ctgtaaagaa taaaaccagt
    20761 gaccggcggg atatggatgc atacaaccga aacaatcatt cattttgtca ccttatactt
    20821 atgtctccat tcccacggtg gaacgggatt ggggtcagcc cacaaacctc gtttttgtga
    20881 gcgagcttcc tgttgtagat ctgccagagc gttatctgtg ttgtaatgct catacaccca
    20941 tgcagcaccc gattgcacca taaagcggtt agcctcacta ccgcctgttg tgaatacacg
    21001 tccgataatg cgaccgtaac ggtcgttgtg tgaatatgtc accctgacgg attgtccggc
    21061 aatcaggctt ttaagttggt cagttgacca gcgtccgtaa gcctgtttct tttccggtgc
    21121 atcaatattg gccagacgaa tccttactgg ctttttattc tgtaaaattt cgatggtatc
    21181 gccgtcaagt acacgaataa cttttccccg tatatccgct gcattggcat atagtgaagg
    21241 cgacaagata tataatgcga atatgattaa tattttcatc aactattgtt ccagttcatc
    21301 aattgttcta aaaataaaca tctctctgtt tttttcatca aaaggcacag gtgtattatt
    21361 cctcattaca gttttgattg agtcgaacag tctgattaat gattctttac ttttggtgtc
    21421 aggcatgacg tacaaagcat aatgccagtt tcctttatct cttgccgtaa gatgactgtt
    21481 tataatgctt atatagcgag cacgagtttt cattgttcgt tctgtttcaa tagccattat
    21541 tgcgccagtt ggttgtttta ttactccatc agggcgatgg gaacagccgg aataacgtgc
    21601 gatgaaggct gcactatcac cgttaatcca ttcagtagcg cctttacttt ccagagatat
    21661 tctcactcgc tgatttaaaa gacgatgctc aagagtccag tattttagtt tgctgggttc
    21721 gaaataacta gggaacgtaa tgtcatcggc ttttacaacc attgctaatc cttgcatagt
    21781 aataccccaa atagttaatt ttctttttga tgatggtatt tcatgtttga taataaaacc
    21841 ttctttaaga gccttgttta gtaatttata tactgggttt ctgctttttt ttgtgaatcc
    21901 taattttctt tttataatgt gaaagtctga atacgtttca tccttaagga aatttaaaag
    21961 acaaagcatg ttgttagctg atattctttt tctttcgtaa aaatcatgaa ttaacatagt
    22021 aaaacctcat aaaccactgg aaggcacatc gttgttattg tgttgttcta tttgttctgt
    22081 ctcaaaagat agcaattcaa tggtttcttt gtttgctggt atatgagaca ttttagttgc
    22141 ttttggtaaa tcttttgatg tgaacacaaa tcctactctt tcaggtagat ttaaaaacat
    22201 attactgtct acataataac tttctgcctg tcttatcatg cggtcagtat caactttttc
    22261 tgttaatgat aagtcggttc tcaccttcct catttcatcg tcaactaata ttgaaccagt
    22321 cattttagct gcccactctg ctgtttctgg attctggatt ttatagatta acttgaattt
    22381 tgcgttttca ataacagcac caattacaga atcaccatta agatcagaag ggcaatctct
    22441 taaatcatct attgcttgga aggccataaa tatatgcaca cttttatctc gtgctgtacc
    22501 taacccttcc attgtcgggc gtgaaatatg atatttcaac tcatcaagaa aaattgcaac
    22561 aacacgtgga gttgtgttta ttctgtctct ggtttcagct atctgaatca aacgcgtaag
    22621 tatcatgcgt tgtgcagaaa taattttttg atttcgggtt gacccaatga tatagcagca
    22681 tcctccatta tcaaaaatct ctttaagaga aaatcctttt tttgcattga cggaatttac
    22741 aagagcaatt tcttctagtt cacctaaaaa tgctggggca tcttctccta ttgattttac
    22801 gttgtcatcg ttgaataaat catacagtgt cattccggtt ttatgaattg cggctacatt
    22861 tctggctgcg cggcgatcag ctatacgata aaaatctgcg gcctctcctt tttttgatag
    22921 actaaatcct gcattaaata attcttctaa ttgttcatgt gatatgtcag caagtaaatc
    22981 aagttgatgg ttggttctgt ttaaattaat tacaacaaat ttctttcctg cttttttaca
    23041 ggcttcccta agaacatgca cagcccattc atcatctttt ggatcttcaa caaaaacagc
    23101 ctcgcctaac agaattgcct gataaagcaa aaccgatgcg gtaatgccct taccggaacc
    23161 tgttgtacct atgattgctg catgttgttt ctgaaattct tttattgtta tatattgcgg
    23221 ttggtcgtct ttgtctaagc caatgaaaac cccttttttc aaatcaatgt aatctagggg
    23281 attgtattct atgctgtctg gcaacaattc ttttactttg cgaacatcgg ttcttgtatt
    23341 tctctcaagt ttgcttttct ttatcattct gtgtttcagg ctgtctattt cacctgctaa
    23401 taatcgtcgc gcggtcaaat gaaaagctaa tccggctagt gtaaagagaa ttgtgattat
    23461 ccataatggt attctaagta gtgtattggg atcgagaacg tcaaataggt agctcagacc
    23521 atgaatacta agtggtgaca ttgtgccgaa tatgaaaaag aaaatggaca cgcacgcaac
    23581 agattttaac cagaagggag cgttttttct ttcctctcgt ggtaggttga atataaatgg
    23641 tagtgttagt cctgccagta atgacaatat gacagggctg ttctgtagcc ataacagaaa
    23701 tattgaaaag gcatcagcaa taccattcag cttttgcatt agatgtggat tcatatttgt
    23761 tttccccatt cataaagtga atctatgggg tacaacaatg gggtacagta atgggggata
    23821 tgatgtaccc catagctgta ccccattgtt gtaccccaat aaaagccaaa aacattgtga
    23881 tcgccttagg cgatcatgcc cgttatccgt tctttcagaa tccagccgat aagttatcgt
    23941 ctggctcctg aaagcccgaa taacgggcaa cggcgtgtgc cgtttcggcc tggctggaac
    24001 gccagtcctt cacgtcacat gggggtaatg gcccgtctgg acgccggacc aaaaccccca
    24061 aggtcaaagg cggcacaccg tcgcgcgcta cgcgcgacca ccccctttaa gacgcgcgtt
    24121 tagcctattt tttcttcgca agctcgaaaa aattggaacg ctgctttaaa gggggtggtc
    24181 gcgcctaatc ggtaatatcc cccgctgggg cgggaaatat tacctggccc gacggtgtgc
    24241 ggggcaagcc ccccacaaaa acc
//
