LOCUS       NZ_CP101220             2337 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-147 plasmid pEC21Z147-2K-2, complete
            sequence.
ACCESSION   NZ_CP101220
VERSION     NZ_CP101220.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593414
            Assembly: GCF_024298765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 104 of 260
            Pseudo Genes (incomplete)         :: 177 of 260
            Pseudo Genes (internal stop)      :: 53 of 260
            Pseudo Genes (multiple problems)  :: 66 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101220.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-147"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z147-2K-2"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            134..559
                     /gene="mobA"
                     /locus_tag="NML26_RS27320"
                     /old_locus_tag="NML26_27325"
     CDS             134..559
                     /gene="mobA"
                     /locus_tag="NML26_RS27320"
                     /old_locus_tag="NML26_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_039023338.1"
                     /translation="MTKNKRSGSEVRQRTKVITLRVNNRIANEIRRRAKSEGLTISEYI
                     RTTSLNNEIKQRVPSRYLDELIQLGRMQKKLFDKGKRPKDKEYLDVMHKIISLCAEMKE
                     VTQRISDIYKKMDLIKEEIKAIKKIHKNKYPGSNLFN"
                     /name="mobA"
     gene            complement(649..1062)
                     /locus_tag="NML26_RS27325"
                     /old_locus_tag="NML26_27330"
     CDS             complement(649..1062)
                     /locus_tag="NML26_RS27325"
                     /old_locus_tag="NML26_27330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_028132811.1"
                     /translation="MGAKFLDQKTGVILEEPEFVKLYIRDLCRVKGLSATQYKIFTFML
                     ANMNTDNVVSFGPRTKEKFLSEHSIKPQTFNNNVSQLIESCLISRIARNEFLVNKKYAV
                     KVDWAKVQKIVWTTTYSNEGIDSTVTIAPKKTD"
                     /name="hypothetical protein"
     Typing          1430..1688
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.151"
                     /coverage="100.0"
                     /evalue="6.999999999999999e-64"
ORIGIN
        1 cgtcgaaaag ttgcagcgga aaatgggaca ggatgtgtat tcgtcatacc gcgaatacat
       61 tcgcttgcat gagaatacct cctgtcaggg caagccccga caccccaaac gtgcaaaatg
      121 gagaatcatt gcgatgacta aaaataagcg tagcggttca gaagtacggc agaggactaa
      181 ggttatcacc cttagagtaa ataacagaat cgcaaacgaa atcagacggc gagcgaagag
      241 cgaagggctg actatttcag aatatataag gacaacaagc ttaaataatg aaataaaaca
      301 gcgcgttcct tccagatatt tagatgaatt aatccaactt ggtcggatgc agaagaaatt
      361 atttgataaa gggaagagac cgaaggacaa agagtatctg gatgtgatgc ataaaataat
      421 ttcactttgt gctgaaatga aagaagtaac acagcgaatt tctgatatat ataaaaaaat
      481 ggatttaata aaagaagaaa taaaagcaat aaaaaaaata cataaaaata aatacccagg
      541 ttccaactta tttaattgag cagaacatag gtcttcataa aaatttaaag acctataata
      601 gaattagcga cattttgttt tatcgaaatc ttttacaatc ataagaaatt aatccgtttt
      661 tttcggggct atcgtcactg ttgaatcaat cccttcatta ctgtaagttg ttgtccatac
      721 tatcttttgt actttcgccc aatcaacttt aactgcgtat tttttattaa ccaaaaattc
      781 atttctggct attctagaaa ttaaacatga ctcaatcagt tgagacacat tattattaaa
      841 tgtttgtggt ttaattgaat gttctgataa aaacttttct ttggttcttg gtccaaatga
      901 aacaacatta tcagtattca tattagcaag catgaacgta aaaattttgt attgcgttgc
      961 tgatagccct ttaacacggc ataaatcacg gatatacagc ttcacgaact ccggctcttc
     1021 caaaattacg ccagtttttt gatctaaaaa tttcgctccc attctcacct cgcaggtaat
     1081 ttagttaccg ttttatcggt atcttatgta tgttccacaa ccgagtaaat actttttttc
     1141 cggtatttga ttagcaacaa aaacagtatt ccggtatttg aaataccgct gagcggtatt
     1201 tcaacctccg ctcagcggta aaaactttcc cgctgagcgg tatatctatt cccgctgagc
     1261 ggtatttagt ttgcctctag gccttgtcac acctgatttt cctatctgtc tgtgaatcta
     1321 tcttagatct cgtgcgtaag tgcactatgc caatactggt tcccttccgc ttcctcgccc
     1381 actcgcgagc ctagcggctc ggtcgctggg gctgcggcga gcggtgccac ctctcgcata
     1441 tcgttgacga tcccgatttg atctcaggcg atcgagtcgc tggccaccgg ttaaacgcgc
     1501 tcaaagcctc ccagacgcac aaaacgcggg ttaaatgccc gtcagggcat ggaaggcgcg
     1561 aaaaacgctt acagaggttt tcagaaggtt aggcgcgccg atacccctaa ccaggggctt
     1621 tcaggtcgct aggaaccgcg ccagccccct gcaaggccca tttgcacgga acagacagcc
     1681 actcggttgg taggtggtcg tagaccgtcg gtgttacagc gatgggggtc tggggttctc
     1741 cccagacggt ttccggttgc gctcaaggcg ctgccggaac cgtggcggct tcgatggtga
     1801 agggtgagcg cgtttccaag aggttttcca ggcaagctat gactgaactg aacagattga
     1861 acgaggagga aacgcgctca gacgctttgt agcgcgcttt tctgtgtcag gcaagtttgt
     1921 tgtgtctttt gcctttcagg ttgttagcag ctggcggagc actgcacggg gattaatgct
     1981 cccattgcta ggatgaaata aagattagga gcgaagttaa gttatatttt taatatataa
     2041 acgcgatatc aataactcac atagttatat tttatgcata tgctaccatc ttgctctttt
     2101 gcctcctgct ctctggacga atcccgcatt accaacaggc aatggattat ataaccaaga
     2161 agatctaatc tttattcctc taataatcca caaacgacag acaacaaaga catcaattaa
     2221 ttcggtgtta tttattaaat aacaagcaat aatttttaaa atctgtgtaa ataatattga
     2281 atgaagcatc atttttttgt atcagaaata ccaggttacg acgcggtatt ttatttt
//
