LOCUS       NZ_CP101219             2353 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-147 plasmid pEC21Z147-2K-1, complete
            sequence.
ACCESSION   NZ_CP101219
VERSION     NZ_CP101219.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593414
            Assembly: GCF_024298765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 104 of 260
            Pseudo Genes (incomplete)         :: 177 of 260
            Pseudo Genes (internal stop)      :: 53 of 260
            Pseudo Genes (multiple problems)  :: 66 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101219.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-147"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z147-2K-1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            complement(join(2048..2353,1..120))
                     /gene="mobA"
                     /locus_tag="NML26_RS27310"
                     /old_locus_tag="NML26_27315"
     CDS             complement(join(2048..2353,1..120))
                     /gene="mobA"
                     /locus_tag="NML26_RS27310"
                     /old_locus_tag="NML26_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_072652687.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRLASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLYELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDIYNQMDLLKQEIKIIKRHHKNKYPGSNTFS"
                     /name="mobA"
     gene            1435..1968
                     /locus_tag="NML26_RS27315"
                     /old_locus_tag="NML26_27320"
     CDS             1435..1968
                     /locus_tag="NML26_RS27315"
                     /old_locus_tag="NML26_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209811.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_072652688.1"
                     /translation="MSKALNKRFSENPFIKDLIIPVKNKQVRISRLGEDNNIMINQSTG
                     EVLGGTSVVTYKKVDSDNFIKLFTQNIKLAFELTQAGQKALFVLFWAVQNKTNNDLVLL
                     DQYTLNDFINENKGLTMSLPTFKRGLGELVKSQILAMNKRKGFYYINPNFVFNGDRVAF
                     TTVLEMNKSSHKDK"
                     /name="replication/maintenance protein RepL"
     Typing          complement(767..1025)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.502"
                     /coverage="100.0"
                     /evalue="6.949999999999996e-69"
ORIGIN
        1 tgtcagtcct tcgttcttcg ctcgccgtct gatttcgctt gcgagtctgt tatttactct
       61 aagggtgata accttagttc tctgccgtac ttctgaacca ctacgtttat ttttagccat
      121 cgcaatgact ctccattttg cacgtttggg gtgtcggggc ttgccctgac aggaggtatt
      181 ctcatgcaag cgaatgtatt cgcggtatga cgaatacaca tcctgtccca ttttccgcta
      241 caacttttcg acgaaaataa aataccgcgt cgtaacttgg tatttctgat acaaaaaaag
      301 atgctttatt caatattatt tacacagatt ttaaaagtta ttgtttgtta tttaataaat
      361 aacgcaaaat taatttaatc ttttgttctt tgtcgttttt tgattattga ctaaataaag
      421 attatgtctt cctgattata taatctattg cctgttagtg atacggagtg cgtccagaga
      481 acaggaggca gaagagcaag atagtagcat gtgcataaaa tacaaaattg tgagttattg
      541 atatcgcgat tatatattaa aaatatgact taatattgct cttaatattt atttttccct
      601 cgcaatggga gcattaatca ccgtacagtg ctccgccaga tgctggcggc ctcaaatcca
      661 ccaagccgcc acggttccgg cagcgccttg agcgcaaccg gaaaccgact ggggagaacc
      721 ccagaccccc accgctgcaa catcatcggt ctgcgaccac ctaccaaccg agtggctgcc
      781 tgtaccgtgc aaacgggctt tgcagggggc tggcgcggtt cctggcgacc tgaaagcccc
      841 tggttagggg tatcggcgcg cctggcttcc tgaaaccctc tgtaagcgtt tttcgcgcct
      901 tccatgccct gacgggcatt tagaccccgt tttgtgcgtt tgggaagttc tgagcgcgtt
      961 taacagatgg ccagcgactc gatcgcctag aatcaaatcg ggatcatcaa cgatatgcga
     1021 gaggtggcac cgctcgccgc agccccagcg accgagccgc caggctcgcg agtgggcgag
     1081 gaagcggaag ggaatctgat tcaacatagt gcacttacgc acagaatttg gcctacgcgc
     1141 ccgcgtccgt gcacacgttt aataagaatt aagaagggaa atcgcgataa accgcgccaa
     1201 tcctagaatc taatgatcag gatattgatc taacgtatca gaatagagca tctaacatat
     1261 caatctcttg atacttaaaa tattaattgt ttgttttttg ctcacctttg atgtgcttta
     1321 attgatattt aaggtatcag aattgtgata ccagtcgatt cggtttattg acatctggag
     1381 attttttctt gatagtcgag gtatcagttt attgatacct gagggtgtaa aataatgagc
     1441 aaggctttaa acaaacgatt tagtgaaaat cctttcatca aagatttgat aattcctgta
     1501 aaaaacaaac aagtaagaat atcgcgatta ggagaagata ataacataat gattaaccaa
     1561 tcaactggag aggttttagg gggaacttcg gtagtaactt ataaaaaagt tgatagtgat
     1621 aattttatta aattgtttac ccaaaatata aaattggctt tcgagttaac acaagctggt
     1681 caaaaagcac tatttgtttt attttgggca gtacaaaata aaacaaataa tgatctagtc
     1741 ttattagatc aatacacgct aaatgatttt ataaatgaaa acaaaggttt aactatgtcc
     1801 cttccaacat tcaaacgtgg gcttggggag ttggtaaaat cacaaatatt agcaatgaat
     1861 aagcgaaagg gattttatta tataaatcct aattttgttt ttaatggtga tagagttgca
     1921 ttcacaacag ttcttgaaat gaataaatcg agtcataaag ataaatgaca atgtattaaa
     1981 acccgccaaa ttggaatttc aaatttattg gcgggtttaa tattcgtgac ggctagcgcc
     2041 gaagatttta gctaaatgtg tttgagccag gatatttatt tttatgatgc cgttttatta
     2101 tttttatttc ctgttttaat aaatccattt gattatatat atcagaaatt cgcttcgtta
     2161 ctattttcat ttcatcgcaa agtaaaatta ttttatgcat cacctcgagg tactctttat
     2221 ccttcggccg ctttccctta tcaaataatt tcttctgcat ccgaccaagt cggattaatt
     2281 catataaata cctggaaggt acgcgctgtt ttatttcatt atttaaactt gccgtcctga
     2341 tatattctga aat
//
