LOCUS       NZ_CP101218             2543 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-147 plasmid pEC21Z147-3K-3, complete
            sequence.
ACCESSION   NZ_CP101218
VERSION     NZ_CP101218.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593414
            Assembly: GCF_024298765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 104 of 260
            Pseudo Genes (incomplete)         :: 177 of 260
            Pseudo Genes (internal stop)      :: 53 of 260
            Pseudo Genes (multiple problems)  :: 66 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101218.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-147"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z147-3K-3"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            134..559
                     /gene="mobA"
                     /locus_tag="NML26_RS27300"
                     /old_locus_tag="NML26_27305"
     CDS             134..559
                     /gene="mobA"
                     /locus_tag="NML26_RS27300"
                     /old_locus_tag="NML26_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000164720.1"
                     /translation="MTKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEYI
                     RTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEMKI
                     VTKRISDVYNEMDLIKDEIKIIKRIHKDKYPGSDLFK"
                     /name="mobA"
     gene            complement(756..1307)
                     /locus_tag="NML26_RS27305"
                     /old_locus_tag="NML26_27310"
     CDS             complement(756..1307)
                     /locus_tag="NML26_RS27305"
                     /old_locus_tag="NML26_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007848.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000007848.1"
                     /translation="MSFGRYKENPFLEGMVVPVKGKQVRLSRLGRDDNILVNQNTGEVQ
                     GTHVTTYKRVDSEQFVKLFTANIGLTFELSSAGIKAFGVLVWSVQNSALSKDEVDLDTL
                     VLEAFLQCQVSASKQPLRLSLATFKRGLNELEKAKIIAKTMRQGRYFINPNFVFNGDRI
                     AFTTVIERKNTIEKQEDKSS"
                     /name="replication/maintenance protein RepL"
ORIGIN
        1 tgtcgaaaag ttgcagcgga aaatgggaca ggatgtgtat tcgtcatacc gcgaatacat
       61 tcgcttgcat gagaatacct cctgtcaggg caagccccga caccccaaac gtgcaaaatg
      121 gagagtcatt gcgatgacta aaaataaacg tagtggttca gaagtacggc agagaactaa
      181 ggttatcacc cttagagtaa ataacagagt cgcaagcgaa atcagacggc gagcgaagaa
      241 cgaaggactg acaatttcag aatatatcag gacggcaagt ttaaataatg aaataaaaca
      301 gcgcgtacct tccaggtatt tagatgaatt aatccgactt ggtcggatgc agaagaaatt
      361 atttgataag ggaaagcggc cgaaggataa ggagtacctc gaggtgatgc ataaaataat
      421 tttactttgc gatgaaatga aaatagtaac gaagcgaatt tctgatgtat ataatgaaat
      481 ggatttaata aaggatgaaa taaaaataat aaaaaggata cataaagata aatatccagg
      541 ttcggattta ttcaaataaa caaataaggt cttttaaaaa agaccttatt tagatgtgca
      601 ttattttatt tcttcggttg ttataatagt ttcaccgtta ggtgaatatt taattctcat
      661 ttcaaactct cgacgctgtt caattatttt tttcattcac cacgagcaaa taaattaggg
      721 ttcgcctgag cggacaccgg ataacccact tattcttaac tagatttatc ctcttgtttt
      781 tcaattgtat tttttctctc aatcacagtt gtaaacgcga ttctgtcacc attaaaaaca
      841 aagttagggt taatgaaata ccttccttgt cgcattgttt ttgcaattat tttcgctttt
      901 tccaattcat taagacctcg cttaaaggtt gcaagactta gtcgtaatgg ttgtttactc
      961 gcagaaactt ggcactgaag aaacgcttct aaaactaagg tatccaaatc aacttcgtct
     1021 tttgagagtg cagagttctg aacggaccaa actaaaacac cgaaggcttt aattcccgca
     1081 gaagatagct caaaagttaa gccaatattt gctgtaaaaa gttttacaaa ctgttcgcta
     1141 tcaacacgtt tatatgttgt tacgtgagta ccttgaacct cacctgtgtt ctgattgacc
     1201 aggatgttat catcacgacc tagacgcgaa agcctaactt gcttcccctt gacaggtaca
     1261 accattcctt ctaaaaatgg gttttcttta tagcgaccga agctcatttt tcacccctaa
     1321 aatgatacgc agaagctaat catagatgcg gggtagctca aaaacaagca taattttgag
     1381 ctacggtagc aacaggattg agctaccgta gtacagatcc tgaaaacacg atccccattt
     1441 catgcgggtt gcgagagatt cccttcttag ttcttaacgg tagtgcgtac gtgctcaatt
     1501 tagctaccgg ttcttttccg cttcctcgtc gattcgctca ctacgttcgg tcaccgactt
     1561 gcggcgagcg gtaacaacac tcgcgcattg ccgtcgatcc cgtttttgat ctgcgacgat
     1621 cgcgccgttt gaaggcctca ggagcaccag aacgcgtttt tgagggtctg tcaggggttt
     1681 gggcgccttt ggcccttaaa cgcgctcaga agctcccaga cgcgatcaaa tgccaacaga
     1741 tgcccgtcag ggcatgggag gcgcgaaaaa tgcttacaga ggctctcagg aggtcaggcg
     1801 cgccgatacc cctaaccagg ggctttcagg tcgccaggaa ccgcgccagc ctcctgcaaa
     1861 gccgattagc acggaacagg aagctactcg gttgggagga ggtcgtagac cgtcggtgtt
     1921 gcagcgatgg gggtctgggg tactccccag acggtttccg gttgcgctca aggcgctgcc
     1981 ggaaccgtgg cggcttcggt ggtgaagggt gagcgcgttt ccaagaggtt ctcgagacaa
     2041 cggcatgact gaactgaaca gatggaacga ggaagaaacg cgctcagacg ctttgtagcg
     2101 cgcttttctg tgtcaggcaa gtttgttgtg tcttttgcct ttcaggttgt cagcagctgg
     2161 cggagcactg cacggggatt aatgctccca ttgccaggat gaaataaaga ttaggagcga
     2221 cgttaagtta tatttttaat atataaacgc gatatcaata actcacatag ttatatttta
     2281 tgcatatgct accatcttgc tcttttgcct cctgctctct ggacgaatcc cgcattacca
     2341 acaggcaatg gattatataa ccaagaagat ctaatcttta ttcctctaat aatccacaaa
     2401 cgacagacaa caaagacatc aattaattcg gtgttattta ttaaataaca agcaataatt
     2461 tttaaaatct gtgtaaataa tattgaatga agcatcattt ttttgtatca gaaataccag
     2521 gttacgacgc ggtattttat ttt
//
