LOCUS       NZ_CP101217             3174 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-147 plasmid pEC21Z147-3K-2, complete
            sequence.
ACCESSION   NZ_CP101217
VERSION     NZ_CP101217.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593414
            Assembly: GCF_024298765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 104 of 260
            Pseudo Genes (incomplete)         :: 177 of 260
            Pseudo Genes (internal stop)      :: 53 of 260
            Pseudo Genes (multiple problems)  :: 66 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101217.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-147"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z147-3K-2"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            complement(380..775)
                     /locus_tag="NML26_RS27285"
                     /old_locus_tag="NML26_27290"
     CDS             complement(380..775)
                     /locus_tag="NML26_RS27285"
                     /old_locus_tag="NML26_27290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222910.1"
                     /translation="MRISNIDWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNELQAQENARKQANQKRMEGKTQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELRYPKDNILQKLKAIRLDLETSPDHH"
                     /name="hypothetical protein"
     gene            955..1908
                     /locus_tag="NML26_RS27290"
                     /old_locus_tag="NML26_27295"
     CDS             955..1908
                     /locus_tag="NML26_RS27290"
                     /old_locus_tag="NML26_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001360358.1"
                     /translation="MATYHLNVKFGGKGQAGNHADYIERKGEYEKRKDLEYSEHGNMPG
                     WARDNPSHFWQAADQFERANGSTYREIEIALPRELTPDQRIELVQDFVRQVAGERHAYS
                     FAIHNPKASIDGGEQPHAHIMMSQRINDGMERSPEQYFRRYNAKNPERGGAKKDSGNLT
                     PTQQKEQLRTLRASWSALQNEHLARHGHRVRVDHRSLEERGIKRTPERHFGFAASSRLD
                     HAQRREILQNRAQPLEHFLEKAHSVRHQFDAQVQQKELVKTLLQDVARERGHALTARIN
                     KTGEVEFERAPTRTRSNTRGHGMGIRDNSRGYDYER"
                     /name="MobA/MobL family protein"
     gene            1920..2450
                     /locus_tag="NML26_RS27295"
                     /old_locus_tag="NML26_27300"
     CDS             1920..2450
                     /locus_tag="NML26_RS27295"
                     /old_locus_tag="NML26_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168766.1"
                     /translation="MASTEEGLLTLLLSVEHLKNTAIEQQKAVADTLAVARETLRGYRD
                     ISDSVVKQVSDEVRKGVQKTDIAGLMGERMTQDFQRLEETVAKMRANAEALNKDIATIH
                     HNVVKEYNTLNKYSLKWFIGVTVSFLVMFSLLGWFTKTLIDRNYTATEVVYQKIVDIEK
                     KLDKPATGKSRKN"
                     /name="hypothetical protein"
     Typing          956..1905
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.685"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgacgcgccc cgtgatcgat ggccttggct ccgtccggca tatcgagagc ggcaaactac
       61 ccgtcccttc actgggaggg gtgagttgtt gccgttccct ccgaccttcc accatccagc
      121 atatcgcacg atcccatgag caccgcaaca actgataact ccagagcaaa aaaaaaaaaa
      181 ggatctgcat catgtgagag tgatcccacc gctcgccgca gccccagcga ccgaacgtag
      241 tgagcgagtg ggcgaggaag cggaagtgaa ccggaaacca cattgagcac gtacgcacct
      301 atgccagatc cgcgcgtagc gcgtcgcgaa atcgaaaaca ttgaacaccg gcaggcaaac
      361 gcaccaccac gcagcggaat taatgatgat ctgggcttgt ttcaagatcg agcctgattg
      421 ccttaagttt ctgtaatata ttgtcttttg ggtatctcag ttcgcctgtt tttgtgtcca
      481 ctagtccggc ctcgatcagt aaccccgccg caagaaacag gcggtgattg cgttcccgtc
      541 gttgtgtttt cccctccatc cgtttttggt tggcctgttt gcgtgcgttc tcctgtgcct
      601 gtagctcgtt tttttctgcg gttgccagga cgtggagtag tttgcgctcc tgttctgtta
      661 gtccggcttc gttatcgagc aggtcaatga tctgcctctg tcgtgcggtc tgttctccga
      721 gcttcctgat aaagccgatt cgtttcttca accagtcgat gttgctgatg cgcatgtttg
      781 ggatgctccc tggtgttttt tgcggtgtgt tggggttgtg cagtatatcg cttgcggtgg
      841 gtgggtgcag gggggtatct tccgtgctaa ttcgtaaaat tagcaggaag cgcacttata
      901 tgtttcttcg ccgttggctc agaaacatgt gcgctcctgc ggagggctaa caccgtggca
      961 acctatcacc tcaatgtaaa atttggcggc aagggacagg ccggaaacca tgccgattac
     1021 atcgaacgca aaggcgagta cgagaaacga aaagatctgg aatactcaga gcacgggaat
     1081 atgccaggct gggcgcgaga taatccctcc catttctggc aagcggctga tcagtttgaa
     1141 cgtgcgaatg gttcgactta tcgggagatc gaaatcgcct tgccgcgtga actgacaccg
     1201 gatcagcgca ttgaactggt gcaggatttt gtgcgtcagg tagctggaga acggcacgcc
     1261 tatagctttg ccattcacaa tccaaaagcg agcattgacg gaggggaaca gcctcatgcg
     1321 catatcatga tgagccagcg tattaacgat ggtatggaac gttcgccgga acagtatttc
     1381 cggcgttata acgccaagaa tcccgaacgg ggaggcgcaa aaaaagattc aggcaatctg
     1441 acaccgactc agcaaaaaga gcaattgcgg acgttacggg cgagctggtc agcgttacag
     1501 aacgagcatt tagcgcgtca tggtcacagg gtgcgcgttg accatcgctc actggaagaa
     1561 cgcggaatta aacgcacgcc agaacggcat ttcggatttg ccgcctcatc acggcttgat
     1621 catgcgcagc gccgcgaaat cctgcaaaac cgagcgcagc cgctcgaaca ttttctagag
     1681 aaagcgcatt cggttcgcca tcaatttgat gcacaagttc agcaaaaaga gttagttaaa
     1741 acgcttttac aggacgtcgc acgggagcga gggcacgcgc ttacggcaag aataaataaa
     1801 acaggtgaag ttgaatttga acgtgcgcca acgcggacgc ggagtaacac gcgagggcac
     1861 ggcatgggca ttcgtgacaa ctcacgcggg tacgactacg aacgttaaaa ggggactgta
     1921 tggcgagtac agaagagggg ttgctcacgc tcttgttgtc cgttgagcat ttgaaaaaca
     1981 ctgcgataga acaacagaaa gcggttgcag acacgctggc ggtcgcaagg gaaacactgc
     2041 gggggtatcg ggatatttct gacagtgttg taaaacaggt tagcgatgaa gtcagaaaag
     2101 gggtacagaa aacggacatt gcggggctga tgggtgagcg catgacccag gattttcagc
     2161 gtcttgaaga gactgtggca aagatgagag ccaacgcgga ggcgcttaac aaggacattg
     2221 cgacaattca tcacaatgtt gttaaagaat acaacacgct caataaatac agtctgaaat
     2281 ggtttatcgg tgttacggtc agttttctgg tcatgttctc gttgcttggc tggtttacca
     2341 aaacgttgat cgacaggaac tacaccgcga cggaggtcgt gtatcagaaa attgttgata
     2401 ttgaaaagaa actggacaaa ccagcaacag ggaagagccg gaaaaactga cgcgcttcgc
     2461 ttgtccaacg cctcacggga cgcgaaaaaa ttccgcgtcc cgttcaccgt tttctgatga
     2521 gaaagtttgc cgttttgacc cgttcggtgc tcagatttgg cgaaattgat tccgaatcac
     2581 ttttgtgaat agttatgcaa gcccctggaa acgtttctga cgcgtttttt gaggtagggg
     2641 tgggggaaag catggttttt tgatgttttg agcgtaaaag cgtgaaaaat cgcgttattg
     2701 tgcttgcgat ctttcgtcag ggagcgtaga acgaaaaaag cccgccgtta ggcgagcttc
     2761 ttcgggttca ttgaggtttt cgacctcctc aatgttcctg tggagtctgt tgcggcgaca
     2821 acggaaacca gttcattcag tttgcatgaa ccttctgtat tcttcaagca ttaccgaccg
     2881 cacagcctcc ccagaatccc gaaaaaatcc tgtttttgca gatgattgcg tgtttgcaaa
     2941 gaccgtttgc ggttggggga gctgtgacga cggcaaccgc acgcgtgaga gttccgctac
     3001 cggcgcagag agtttcatcg acagcggaca cctgatgagt tgccacaaaa accgtatgta
     3061 gcgcggctag attggttgag ttagaccttg gcggcagttc ccggtatgtg gtggcgtgac
     3121 gggtgagatg gtgcggcgaa gacgcatacg tcacgcaggc gcgggttctt ccac
//
