LOCUS       NZ_CP101216             3375 bp    DNA     circular CON 26-JUL-2023
DEFINITION  Escherichia coli strain EC21Z-147 plasmid pEC21Z147-3K-1, complete
            sequence.
ACCESSION   NZ_CP101216
VERSION     NZ_CP101216.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29593414
            Assembly: GCF_024298765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Prevalence and genetic characterization of carbapenem- and colistin-
            resistant foodborne pathogen Escherichia coli from patients in a
            hospital in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Ma,J., Tang,B., Lin,J., Wu,J., Dong,Y., Yang,H. and Yue,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2022) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, No. 198
            Shiqiao Road, Hangzhou, China 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. June-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/26/2023 05:44:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,481
            CDSs (total)                      :: 5,363
            Genes (coding)                    :: 5,103
            CDSs (with protein)               :: 5,103
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 104 of 260
            Pseudo Genes (incomplete)         :: 177 of 260
            Pseudo Genes (internal stop)      :: 53 of 260
            Pseudo Genes (multiple problems)  :: 66 of 260
            Pseudo Genes (short protein)      :: 4 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP101216.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC21Z-147"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC21Z147-3K-1"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-06-08"
     gene            complement(563..754)
                     /locus_tag="NML26_RS27260"
                     /old_locus_tag="NML26_27265"
     CDS             complement(563..754)
                     /locus_tag="NML26_RS27260"
                     /old_locus_tag="NML26_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            818..1141
                     /locus_tag="NML26_RS27265"
                     /old_locus_tag="NML26_27270"
     CDS             818..1141
                     /locus_tag="NML26_RS27265"
                     /old_locus_tag="NML26_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024132211.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1350..>1790
                     /locus_tag="NML26_RS27270"
                     /old_locus_tag="NML26_27275"
                     /pseudo=""
     CDS             1350..>1790
                     /locus_tag="NML26_RS27270"
                     /old_locus_tag="NML26_27275"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1819..2379
                     /locus_tag="NML26_RS27275"
                     /old_locus_tag="NML26_27280"
     CDS             1819..2379
                     /locus_tag="NML26_RS27275"
                     /old_locus_tag="NML26_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032329292.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISGHTTSVKQQLDTLSATVSTQFSTTEAELSRQQKKLLWQVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2386..2619
                     /locus_tag="NML26_RS27280"
                     /old_locus_tag="NML26_27285"
     CDS             2386..2619
                     /locus_tag="NML26_RS27280"
                     /old_locus_tag="NML26_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          399..482
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.059"
                     /coverage="95.0"
                     /evalue="8.83e-18"
     Typing          1132..2702
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.817"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgacgcgccc cgtgatcgat ggccttggct ccgtccggca tatcgagagc ggcaaactac
       61 ccgtcccttc actgggaggg gtgagttgtt gccgttccct ccaacctttc accatccggc
      121 atatcgcacg atcccatgtg caccgcaact cctgataact ccagtgcaaa aaaaaaaaaa
      181 acggatctgc atcacaacga gagtgatccc accgctcgcc gcagccccag cgaccgaacg
      241 tagtgagcga gtgggcgagg aagcggaaga gtcacagatg caagcattgt gcacttacgc
      301 gcctgtgcca gtgtccgcgt gtgtgctgtt tatcgggtgc gctgttttta catctgtgcg
      361 gctttttact cctgacatgg tgcacttccc atccatgtct gcaccgatgc aaaatgagat
      421 aagccagtat acacatcgct atcgctctgt gaactgggtc agggctgcgc cccgacaccc
      481 gcactgacgc gccctggcgg gcttgtctgc tcccggcatc cgctcacagg caagctgtga
      541 acgtctccgg gagctgcatg tttcagttgc tttcaccgtc atcgccgaag cgtgcgaggc
      601 aactgcggta gagttcgtcg gcgtggtcgt gaagtgattc gcagatgtct gcctgttcgt
      661 ccgcgtccag atcgttgagt ttttccagca gggttagcgt ctggcttctg ataaagcgag
      721 ccatgttaag ggctgttttt tcctgtttgg tcatggtctt aaggctcctt gtatggggga
      781 tttgttaggg ggaatgatac cgatgaaacg cgagaagatg ctcacgatac gggttaccga
      841 tgaggagcac gcccggttac tggaacgctg cgagggtaaa cgactggcgg aatggatgcg
      901 gcgggtctgt ctgggggagc cggttgcacg cacaggaaaa ctgccgacac tatcgccgcc
      961 attgctgcgg catctggctg ccatcgggaa caacctgaac cagacagccc ggaaggtgaa
     1021 cagcggccag tggtcttcga ttgaccgggt tcatgtggtg gccgcactga tggccatcga
     1081 gggtgaattg cgccagttgc ggcaggctgt cagggaacag ggggtacggg atgatagtta
     1141 aatttcatgc cagggggaaa ggcggtggaa gtggcccggt cgattacctg ctggggcggg
     1201 agcgtaaccg cgaaggcgca acggtgctcc ggggtaaccc ggaagaagtg cgggaactca
     1261 ttgattccac gccattctcg aagaaataca cttcaggcgt tctgtcgttc gcggagaagg
     1321 agttaccgcc gggtgaacgc gaaagagtga tgacgagctt tgagcgtgtt cttatgcccg
     1381 gtctcgaaaa agaccagtac agcattctct gggtggaaca ccaggataag gggcggctcg
     1441 aactgaattt tgtcatcccg aacatggagc tacagagcgg taagcgcctc cagccgtact
     1501 acgaccgcgc ggacaggccg aggataaatg cctggcagac gctggttaat catcattacg
     1561 ggctgcgtga cccgaacgca ccggagaaca gacggacgct tgtcacgcca aataatctgc
     1621 caaaagcgaa acaggaggcc gcagaagcga ttacacgggg tcttgaggcg ctttaccatg
     1681 ccggagcaat aaaaacgcgt caggatgtca ctggggcgct cacagcggca ggttttgagg
     1741 tggtgaggac aaccagaagc agtatcagta ttgccgaccc ggagggtggg cggaatctca
     1801 ggctaaaggg ggcgctgtat gagcaatctt ttacaaatgg gaacagactt cgagaagaaa
     1861 ctgaaagagc gagccgcttc taccgagaac atgctgaaca gcgagttcag gaagctcgaa
     1921 gagtctgtag acaaggcact gagcttaaac aggcagaaaa tcagagacgc tatcagcgga
     1981 catacaacat ccgtgaaaca gcaactggat acactgagcg ccaccgtgag cacgcagttc
     2041 agcaccacag aggccgaact gtcccggcag cagaaaaaac tgctctggca ggtgatcaaa
     2101 gggcggatac tgtttccggc gctgaccgcg ctcagcgtca cgggcggcat tttcctgggg
     2161 tgctgggggc tgatggagtg gcaggaaagc aagattgcga aaaatatcct gactatcaga
     2221 gagcaggaaa acacgctggc gaaactcgag gcaaaaacct ggggggtgac gttcgtcaat
     2281 ggcgagaacg ggaaatttct ggtgttgccg gacggggtga aaggggaaaa cacctggacg
     2341 gtgggcgaca agaacgccgt gaggctggtc agggagtaaa cgagtatgac ggaactggaa
     2401 gggcatttgc tgaacgcctt agagcatttg caacaggact atatgcggcg gctgaacgag
     2461 tgggagagcg ccttcgcgga attgcagaag atgcacgagg ttacgcaacg gaacaacgcg
     2521 atactgaacg agcgcgtcgt gatattgagt cagcaggtgc agagattagc cgggcaagtg
     2581 gatcgcttga gtcggttatt tatcgcgaac aacagctaag ggatgaacag gagcgtgccc
     2641 ggtacagaca tgaggtacag gaacgcgcgg cacgggttcg gagtgggcca tcaatggaga
     2701 tgtgaccggg gaagtgacga aatcttgcca ttttgaccag ttcgacggct ggggcgagcg
     2761 aaacaaatcg tgtgccgtat ttgtgcatag ctatgcaaca tcctgaaaac gcatttcacg
     2821 cgtttttggg gattcagcca ggggaaaaca tggttttttt actttttgag cgtaaagacg
     2881 tgaaaaatct cgttattgca cttgcgatct ttcgtcggcg ggcgtagaac gaaaacagct
     2941 cgccaggaag gcgagctgtt tcgggttcat tgaggtctct gacctcctca atgttcctgt
     3001 ggaaactgtt gagatccaca acagaaacca gttcattcag tttgcatgag ccttctgtat
     3061 tcttcaagaa tttttcaaca gcacagtctc ccaggaaccc cgaaaaaaac ctgtttttgc
     3121 agatacttgc gtatttgcga gaatcgtttg cggttggggg agctgtgacg acggcgaccg
     3181 cacgcgtgag agttccgcta ccggcgcaga gagtttcatc gacagcggac acctgatgag
     3241 ttgccacaaa aaccgtatgt agcgcggcta gattggttga gttagacctt ggcggcagtt
     3301 cccggtatgt ggtggcgtga cgggtgagat ggtgcggcga agacgcatac gtcacgcagg
     3361 cgcgggttct tccac
//
